Objective To evaluate the cause and treatment of complication of percutaneous intra-arterial femoral port-catherter system(PCS) implatation. Methods Two hundreds and two patients with malignant tumors in the thorax, abdomen, pelvis and limbs were treated by using chemotherapy and lipiodol embolization via PCS. The related complications rate was 17.3%(35/202). Results The complication included incision infection delayed healing, and wound dehiscence 17.1%(6/35); local hemorrhage 5.7%(2/35); falling down and detachment of catheter of PCS 5.71%(2/35) and 2.86%(1/35) respectively loosening 2.86%(1/35) and changing direction 2.86%(1/35). The indwelling catheter blockage was 31.4%(11/35) and migrating of catheter tip was 28.6%(10/35). Most of these cases were recovered after appropriate management without any fatal and serious outcomes. Conclusions The Technique of percutaneous intra-arterial femoral port-catheter system implantation is safe and reliable. The related complication is slight and easy for management.

Objective To assess the efficacy of nebulized budesonide and salbutamol in the treatment of asthma by Meta‐analy‐sis .Methods According to the requirements of Cochrane systematic review ,a through literature search was performed among Wan‐fang database ,CNKI database ,VIP database of Chinese Periodicals and Pubmed database .Meta‐analysis was conducted on a total of 4 649 patients involved in 51 papers that met the inclusion criteria by RevMan4 .3 software .Results Compared with control group (RR=1 .27 ,95% CI=1 .23-1 .32) ,budesonide group which was treated by atomization inhales of nebulized budesonide based on routine treatment(RR=1 .31 ,95% CI=1 .16-1 .49) and salbutamol group which was treated by atomization inhales of salbutamol based on routine treatment(RR=1 .23 ,95% CI= 1 .17 -1 .28) ,the combined group which was treated by atomization inhales of nebulized budesonide and salbutamol based on routine treatment exhibited higher rates of total effectiveness with significant inter‐group difference when taking rates of total effectiveness as index(P<0 .01) .When taking hospitalization rate as index ,the result of the combined group was lower ,compared with the salbutamol group (RR=0 .34 ,95% CI=0 .13-0 .90) ,and the intergroup differ‐ence was significant(P<0 .05) .Conclusion The effect of nebulized budesonide and salbutamol in the treatment of asthma is cer‐tain .

Objective To explore the effects of collaboration intervention on self-efficacy and self-perceived burden in stroke patients. Methods 92 stroke patients hospitalized in the neurology department from January to June, 2014 were divided into control group (n=46) and intervention group (n=46). The control group received routine nursing and the intervention group received collaboration intervention in addi-tion. They were assessed with Self-Efficacy for Managing Chronic Diseases and Self-perceived Burden Scale before and after intervention. Results There was no difference in the scores of self-efficacy and self-perceived burden between groups before intervention (t<0.585, P>0.05). The score of self-efficacy was more in the intervention group than in the control group (t=3.483, P<0.001) after intervention, and was less of self-perceived burden (t=-5.698, P<0.001). Conclusion The collaboration intervention can improve self-efficacy and relieve self-per-ceived burden in stroke patients.

ObjectiveTo investigate different personality traits and psychological interventions'effect on the patients' anxiety in fiberbronchoscope examination.MethodsThe eysenck personality questionnaire(EPQ)scale and Zung self-rating anxiety scale(SAS) were used to assess the patients.The patients were randomly divided into experimental and control groups,the experimental group,respectively,the implementation of cognitive therapy and relaxation treatment of psychological intervention,psychological evaluation was investigated again before fiber bronchoscopy.ResultsThe scale showed that it had significant difference(P＜0.05 ) between before and after the psychological intervention( before intervention:55.8 ± 1.50,54.9 ± 1.62,57.84 ± 2.13 ; after intervention:46.2 ± 1.38,45.3 ± 1.47,59.26 ± 2.41 ).The patients'personality trait was correlated with their anxiety(P＜0.01 ).ConclusionsThe psychological intervention can be effective in reducing patients'anxiety,and it can help patients take the initiative to meet the inspection,improve the success rate of inspection.And different personality traits can effect on patients' anxiety level.

In this study,we presented data that K562,YAC-1 and LAC-1 cells or tumor-membrane-associated protein could stimulate BCG-treated macrophages from BALB/c mice to produce cy-totoxic factor (M?-CF). CF-inducing activity of K562 membrane or tumor cells remainde afterheat-treated at 100℃ for 20 min, but decreased by trypsin digestion or NaIO_4 oxidixa-tion. Thisactivity was also hibited by ConA or D-Man, but not by Gal. and Giu. The results suggested thatgiycoprotein containing D-Man on tumor cell surface was responsible for traggering M?s toproduce CF.

OBJECTIVE:To develop a capillary electrophoresis method for determing the enantiomer of ceftriaxone Na.METHODS:A chiral resolving agent,?-cyclodextrin,was employed as chiral additive for ceftriaxone Na enantiomeric separation by capillary electrophoresis.In different electrophoresis polarity mode,the effect of pH of background electrolyte and the concentration of ?-cyclodextrin were investigated.RESULTS:The optimal conditions for enantiomeric separation were as follows:separation voltage:28kV,buffer solution:NaH2 PO4 50mmol/L,?-CD 0.04mmol/L,Tirs 3.0mmol/L,pH7.15.CONC_LUSION:The method is simple,sensitive,rapid and accurate,and can be used for the quality control of ceftriaxone Na enantiomers.There was significant difference in contents of enantiomers of ceftriaxone Na between products of two factories.We suggest that a quality control method for ceftriaxone Na enantiomer should be established,which will provide scientific basis for quality control of drug and clinical choice of effective antibioties.

As a wide field of internet industry, the internet medical is sprouting prosperous with many advanta-ges still, namely promoting the medical resources integration and earlier disease prevention, providing more conven-ient health care services, etc. , but there are still many limitations. By analyzing the role of internet in the promotion of medical services, this article focused on the internet limitations. Ultimately, we put forward we should calmly oper-ate internet and make it as the auxiliary tool for medical care services instead of relying too much on it in order to pro-vide a reference and a basis for the prosperous development of internet medical.

Objective To study the method for determination of nipacide PX and triclosan in disinfectant by high performance liquid chromatography. Methods High performance liquid chromatography, based on the mobile phase (CH3CN:H2O=60:40, pH=3.0) and diode array detector(DAD, ?= 280 nm), was chosed to determine nipacide PX and triclosan in disinfectant. Results The detection limit of nipacide PX was 2.48 ng and triclosan was 2.08 ng. The rate of recovery was 95.4%-103.3%,the relative standard deviations(RSD) was 0.6%-3.4%. Conclusion The method is simple, precise and suitable for the determination of nipacide PX and triclosan in disinfectant.

Objective To evaluate the clinical diagnostic value of coronary artery calcification score(CACS)of multi-slice spiral computed tomography(MSCT)combined with carotid intima-media thickness(IMT)measure in elderly patients with coronary artery disease(CAD). Methods　CACS of MSCT,carotid IMT measure,and coronary angiography were performed in 68 patients,including 36 cases with CAD(CAD group)diagnosed by coronary angiography and 32 cases(control group)with coronary arterial stenosis(＜50% stenosis).CACS and carotid IMT were compared between two groups. Results　The coronary artery calcification score was significantly increased in CAD group compared with the control group[(349.5±86.3)vs.(74.7±25.2),t=13.670,P＜0.01],and it was increased with the severity of coronary arterial stenosis.The carotid intima-media thickness in CAD group showed significant difference with that in control group[(1.11±0.05)mm vs(0.69±0.13)mm,t=13.587,P＜0.01].In CAD group,CACS exhibited a significant positive correlation with carotid IMT(r=0.950,P＜0.01).The positive rates of CACS and carotid IMT were both 77.8% (28 cases)in CAD group and both 12.5%(4 cases)in control group,which showed significant difference between two groups(X2=28.976,P＜0.01). Conclusions　CACS of MSCT combined with carotid IMT have high sensitivity and specificity in evaluating coronary arteriaI stenosis.It can be used as a non-invasive examination to diagnose CAD in the elderly.

Objective To observe the influence of N-acetylcysteine(NAC)on the formation of Pseudomonas aeruginosa biofilm(BF),and to study the synergism of bactericidal activities of NAC combined with levofloxacinon to the mature Pseudomonas aeruginosa biofilm.Methods The experiment was done in experiment centre of Xiangya Hospital.Scanning electronic microcopy(SEM)were utilized to demonstrate the effect of NAC on the formation of biofilm.Then biofilm cultured without NAC were detected by optical microscopy after being stained with AgNO3.The minimal inhibitory concentration were detected by double test tube diluted method.The mature biofilm was treated by NAC and/or different concentrations of levofloxacin for 24 hours.The influence of levofloxacin combined with NAC were examined by MTT method on the bacterial quantity in biofilms.Results Biofilm was achieved on the silicon slides in all groups 7 days after the incubation of Pseudomonas aeruginosa.Scanning electronic microcopy showed that the mucoid materials among bacteria was significantly reduced and the thickness of biofilm was decreased in NAC groups.Levofloxacin beyond 1 MIC could significantly decline the viable cells on biofilms(P