1.The relationship between the apolipoprotein M-sphingosine-1-phosphate axis and atherosclerosis
Chinese Journal of Laboratory Medicine 2016;39(12):983-987
Apolipoprotein M-sphingosine-1-phosphate axis ( apoM-S1P axis ) signaling pathway consists of apolipoproteinM (apoM), sphingosine-1-phosphate (S1P) and sphingosine-1-phosphate receptor (S1PR).Plasma apoM belongs to lipocalinsuperfamily members , and is mainly associated to high density lipoprotein( HDL), whereas HDL-cholesterol correlates inversely with cardiovascular risk .The ability of apoM to bind S1P is due to a lipophilic binding pocket within the lipocalin structure of the apoM molecule . S1P, a bioactive mediator of phospholipid metabolism , predominantly abound in HDL among all lipoproteins.S1P can not only be used as intracellular second messengers , but also as intercellular signal molecules, activating of G protein-coupled receptors (S1PR) to mediate various physiological functions.It′s clear that apoM protects human beings from atherosclerosis .Furthermore, recent studies showed that S1P has a significant impact on atherosclerosis , and ApoM-S1P axis may play a important role in the pathogenesis or progression of atherosclerosis .
2.Effects of insulin-like growth factor-1 at varied levels on pregnancy outcome in pregnancy maternal serum and cord serum of patients with PCOS
The Journal of Practical Medicine 2016;32(1):73-75
Objective To detect the effect of insulin-like growth factor-1 (IGF-1) at varied levels in pregnancy maternal serum and cord serum of patients with the polycystic ovary syndrome (PCOS) on pregnancy outcomes, and explore whether IGF-1 could be used as a diagnostic marker for PCOS. Methods From January 2012 to December 2013, pregnancy maternal and cord serums were collected from 120 PCOS patients and 120 normal pregnant women in the Department of Obstetrics and Gynecology of our hospital. ELISA was used to detect the levels of IGF-1, and analyze the levels of IGF-1 in different pregnancy outcomes between normal pregnant women and PCOS patients. Results Compared with the control group, the levels of IGF-1 in pregnancy maternal and cord blood were increased significantly in patients with PCOS (P< 0.05). The incidence of stillbirth, premature delivery and macrosomia was significantly higher in PCOS patients. The levels of IGF-1 in pregnancy maternal and cord serum were significantly higher in all PCOS patients with adverse pregnancy outcomes compared with the control group (P < 0.05). Conclusion IGF-1 could be used as a risk prediction marker for pregnancy outcome in patients with PCOS.
3.Surveillance of minimal residual disease in acute B-lineage lymphoblastic leukemia
Journal of Leukemia & Lymphoma 2013;22(4):248-250
Minimal residual disease (MRD) is the cause of relapse in leukemia,and its dynamic monitoring has been a hotspot yet proven to be difficult.MRD in acute B-lineage lymphoblastic leukemia has its own characteristics,normal bone marrow produces a large number of B precursor cells,easily mistaken for leukemia cells,and there were no specific gene alterations that can be conveniently detected.This paper summarizes the antibody combinations in flow cytometry,timing for surveillance and polymerase chain reaction for gene detection,and provide a reference for B lymphocytic leukemia MRD monitoring.
4.Teh immediate-early gene c-fos and opilepsy
Journal of Medical Postgraduates 2001;14(2):169-172
C-fos,one of the immediate-early genes,encodes Fos,which appears to modulate the express of more “downstream” genes and attribute to long-term changes in cellular function.Recently,c-fos has been researched on extensively and progress has been acquired greatly.This article summarizes c-fos on structure,function and the relationship to epilepsy.
5.Advances of PRDM genes and DNA methylation in cancer
Yongli TAN ; Shuangxiang TAN ; Ruicheng HU
Journal of International Oncology 2013;(3):174-177
PRDI-BFI and RIZ homology domain containing proteins (PRDM) play a key role in cell differentiation and proliferation.Most members of the PRDM gene family are tumor suppressor genes which involved in tumorigenesis and abnormal expression in a variety of tumors.Aberrant DNA methylation often silences these genes,which may occur in the early stage of tumor,Cancer can be reversed by demethylation,which provides a new way for cancer treatment.
7.Current research of the role of Sox2 in Glioblastoma Multiforme
Jinman GUO ; Chao TAN ; Huojun HU ; Yuan TAN
Tianjin Medical Journal 2015;(12):1467-1469
Glioblastoma (GBM) is one of the WHO gradeⅣmalignancies, which is an acentral nervous system cancer with poor prognosis unless the lesion is in the brain stem. The incidence of GBM accounts for 80%of human primary malignant tumors in brain. Only 5%GBM can survive up to 5-years. Many researches showed that Sox2 is a pluripotent regulator, and muta?tion or abnormal function of Sox2 are closely related to the development of GBM. There are studies demonstrated the possibil?ity of using Sox2 gene as apotential target for GBM therapy. This paper reviewed recent progress in GBM.
8.Research progress of MicroRNAs involved in the tumor microenvironment regulation in non-cell-autonomous mechanisms
Jinman GUO ; Chao TAN ; Huojun HU ; Yuan TAN
Tianjin Medical Journal 2016;44(2):247-250
As an internal environment of tumor occurrence, tumor microenvironment is composed of a variety of cells and extracellular matrix, and plays a crucial role in tumor formation, transfer and resistance to drugs. The regulation of tumor microenvironment will be a potential target to control the cancer. MicroRNAs (miRNAs) are a kind of 21 to 25 nucleotides single-stranded RNA, and are mainly involved in regulating gene expression. Recently, with the suggestion of cellular auton-omous tumor inhibition mechanism, the regulation of tumor microenvironment by miRNAs has received great attention. This review summarizes recent findings on the non-cell-autonomous mechanisms of miRNAs-mediated regulation of tumor micro-environments, which provides foundations and perspective on the design of therapeutic interventions.
9.Effects of TNF alone and Incombination with Antitumor Chemicals on Carcinosarcoma in vitro
Hongwei GUO ; Wenxiang TAN ; Yi HU
Chinese Journal of Cancer Biotherapy 1994;0(01):-
Objective: To investigate the effects of rhTNF alone and in combination with antitumor chemicals on Walker-256 cell line in vitro. Methods: the cytotoxic activity in vitro were be examined by MTT method and the flow cytometry(FCM) analysis and alteration of every phase of cell cycle for rhTNF or (and) Adriamycin(ADM) against Walker-256 cell line. Results: rhTNF had a strong activity of antitumor in vitro and good dose-effect relationship(r=0.9811). Compared with negative control group, the effect of rhTNF was significant (P
10.New progress inhypoglycemic mechanism and clinical application of Berberine
Xueying TAN ; Linshuang ZHAO ; Jingbo HU
Chinese Journal of Diabetes 2015;(12):1131-1133
[Summary] Berberine is akind of alkaloids extracted from Chinese herb medicine in cludingphellod endron ,coptis and radix berberidis .In recent years ,the pharmacological effects of berberinewas investigated extensively including anti-infection ,anti-arrhythmia ,protection of ischemic myocardium ,anti-hypertension , anti-tumor ,anti-HIV .And there are increasing reports about its hypoglycemic effect ,but its mechanism remains unclear .Here we summarize the possible mechanism of hypoglycemic effect and clinical efficacy of berberine .