Objective To investigate the lentivirus-mediated RNA interference(RNAi) reduced human epidermal growth factor 2 (HER2) gene expression in ovarian cancer chemosensitivity.Methods Specifically targeted short hairpin RNA HER2 gene ( shRNA) lentiviral expression vector and negative control sequences lentiviral vector were constructed.stable expression of HER2-shRNA in SKOV3 cell line ( KD) and negative control cell line (NC) were established.The uninfected SKOV3cell lineserved as blank control(CON)group, sensitivity to changes in three kinds of cells to cisplatin chemotherapy in the HER2 gene expression was detected by RT-PCR and Western blot technique, CCK-8 assay CON and KD cells strains were established xenograft model in nude mice in each group to detect the expression of the HER2 protein, as well as by cisplatin chemotherapy tumor size. Results Expression results showed that a stable cell line HER2-shRNA and negative control cell lines had been successfully establish by fluorescence and flow cytometry.RT-PCR results showed KD cells relative HER2 mRNA expression levels compared with the NC group, the difference was statistically significant (P<0.01), whereas no significant difference when compared with the CON group, HER2 mRNA KD Group inhibition rate was (95.5 ± 0.12)%.After transduction specific HER2-shRNA ovarian cancer tumor cells, HER2 protein expression was significantly reduced, growth had slowed down significantly after cisplatin treatment ( P<0.01 ) .Conclusion Lentivirus-mediated HER2-shRNA can effectively inhibit the expression of the HER2 gene, increase chemosensitivity to cisplatin in ovarian cancer.

Objective:To study the clinical value of serumsoluhle CD8 antigen(sCD8)inpatients withGraves disease(GD).Methods:The level of serum sCD8 was detected by ELISA in40 healthy controls and 46 GD Patients.Serial measurement were carried out in 28 patientsbefore and after trea treatment with PTU. Results: Serum sCD8 levels were increased signifiantly in untreated GD patients as compared with conrols(21.91 ±9.13 kU/Lvs9.84±3.08kU/L, P ＜0.001).After treatment with PTU for 2 to 7 months,sCD8 levels tetumed to normal when eu-thyroidism was esta established in 22 patients(9.67 ± 5. 14 kU/L) but remained high in 6 unmiproved patients(38.74 ± 9.89 kU/L). No statisticalrelationship was found between sCD8 and T3、T4、TSH、TGA、 TMA.Conchusion: The level of serum sCD8 correlate well with disease activityand may be of value in disease prognosis. PTU show some suppressor effect on immune.

Objective To study the mechanism of depression by explore the expressional differences of synapsin and dopamine transporter(DAT) mRNA of hippocampus in depression model rats. Methods Depression model were replicated by chronic stress. 20 rats were randomly divided into normal control group(n=10) and de-pression model group(n=10). Synapsin and DAT mRNA expressions in hippocampns were detected with RT-PCR technique. Result The values of synapsin and DAT mRNA in hippocampus of normal control group were 0.84±0.08 and 1.24±0.08, respectively, and 1.24±0.12 and 1.85±0.09 of model group, their t values were 8.87 and 16.50 (P<0.01). Conclusion The expressions of synapsin and DAT mRNA in hippecampus of depression model rats were increased significantly.

The separation and purification of beta chains of globm from the inbreeding SMMC/B mice were performed by the dissociation of chains in urea, CMhcellulose column chromatography for the separation of alpha and beta chains, and Sephadex G25 column chromatography for removing salts. The pure beta-chain was digested by trypsin. Digested fragments were resolved by cellulose membrane two-way electrophoresis, thus obtaining the map of spots of beta-chain fragments for B33, B34, Bp(), Bp and Bf. The spots,BTp3, which have the same positions ,were separated and micro-sequences were assayed by DABITC/PITC double coupling technique. According to the genetic code, we might deduce the nucleotide sequence of their mRNA from the known amino acid sequence of BTp3, a piece of beta-chain. By analyzing the altered genetic loci of beta-chain of globin, we might determine the genetic purity of the strain and distinguish objectively the genetic types between species and subspecies.

Objective To investigate the clinical effect of combination of compaction and psychological intervention on osteoporotic thoracolumbar compression fractures. Methods Two groups of patients with osteoporotic thoracolumbar compression fractures were treated with percutaneous kyphoplasty, and the control group was treated with dense and auxiliary treatment. The changes of NRS scale scores before and after treatment in two groups of osteoporotic thoracolumbar compression fractures were recorded. The data were input into SPSS software and analyzed and concluded. Results Two groups of osteoporotic thoracolumbar compression fracture patients before treatment NRS score had no significant difference between; NRS scores in study group is lower than before significantly better than the control group, comparing the data of P<0.05. Conclusion Osteoporotic thoracolumbar compression fractures underwent surgical treatment after Aclasta, combined with psychological intervention can significantly improve clinical efficacy, has positive significance to guarantee the quality of life of patients, life safety.

[Objective] To discuss the pathogenic bacteria distribution and drug resistance of patients of infectious mononucleosis combined with bacterial in-fection. [Method] Choose 38 children patients as research objects who were diagnosed as infectious mononucleosis with positive throat swab culture, and make investigation to the separated pathogenic bacteria distribution and drug resistance.[Result] In the 38 cases of samples, there ’re 44 strains of pathogenic bacteria detected(2 kinds of pathogenic bacteria detected in 6 cases respectively), the front 3 were: 14 strains of staphylococcus aureus(31.8%), 10 acineto-bacter baumannii(22.7%) and 8 klebsiel a pneumaniae(18.2%); the staphylococcus aureus had highest drug resistance to Penicilin G, occupying 85.7%; to ampicil in, cefoxitin and macrodantin, the acinetobacter baumannii had drug resistance 100%; the klebsiel a pneumaniae had drug resistance of 100% to ampicil in.[Conclusion] The pathogenic bacteria distribution and drug resistance of children patients with infectious mononucleosis combined with bacteria infection have definite features, which shal be emphasized in diagnosis and treatment.

OBJECTIVE To improve efficiency of hospital infection administration and take strict precautions against medical malpractice in Chinese level Ⅱ hospital of UN Peacekeeping Army in Lebanon.METHODS The current situations of its hospital infection administration were enumerated,compared and analyzed,and corresponding measures of administration were suggested.RESULTS Under the influence of characters of medical missions during peacekeeping,the hospital infection administration presented the problems that patients and disease spectra were diversified,the rate of hospital infection was influenced by peacekeeping mission,the positive rate of HIV was high,medical workers protection awareness was lagged behind,and the disposition of hospital was illegitimate;many measures should be taken to enhance the administration level.CONCLUSIONS In order to well develop the hospital infection administration in Chinese level Ⅱ hospital of UN Peacekeeping Army in Lebanon,we should reform and improve the system of hospital infection administration,and establish an effective and systematic prevention system of hospital infection.

Objective:To prepare N-trimethyl chitosan (TMC)-coated sparfloxacin (SL) nanoliposomes in situ gels(ISG)and in-vestigate the drug release in vitro.Methods:SL liposomes were prepared by a pH gradient method , and then homogenized to nanolipo-somes by high pressure .TMC was used as the coating material to prepare TMC-coated SL nanoliposomes .Poloxamer 407 was used as the gel base, and the optimal amount was selected according to the gelation temperature .TMC-coated SL nanoliposomes ISG was pre-pared using a cold method , and the morphology , size, zeta potential and entrapment efficiency of TMC-coated SL nanoliposomes were studied.A membraneless model was used to study the drug release in vitro, and the result was compared with that of TMC-coated SL nanoliposomes.Results:The optimal amount of poloxamer 407 in the formula was 25%, and the gelation temperature was 23.6℃in the artificial tears and 33 .5℃in the diluted artificial tears .The morphology of TMC-coated SL nanaoliposomes in the ISG was spherical with the mean diameter of (96.8 ±1.5) nm, zeta potential of (46.2 ±1.4) mV and entrapment efficiency of (76.6 ±2.4) %, and the indices had no significant difference when compared with those of TMC-coated SL nanoliposomes .Both the drug release in vitro and gel dissolution profile of TMC-coated SL nanoliposomes ISG exhibited the characteristics of zero-order kinetics, and compared with that of TMC-coated SL nanoliposomes , the sustained release property of the ISG was more significant .Conclusion:TMC-coated SL nanoli-posomes ISG has promising gelation temperature and notable sustained release property .

Objective To investigate the risk factors of pneumonia in patients with non ST segment elevation-acute cononary syndromes (NSTE-ACS).Methods A total of 183 cases with NSTE-ACS was divided into pneumonia and non-pneumonia groups.The possible risk factors were screened firstly,then nonconditional logistic model multivariate analysis was used to investigate risk factors including age,sex,weight,smoking,chronic obstructive pulmonary disease (COPD),hypertention,diabetes,left ventricular failure,renal failure,low serum albumin,early revascularization and early rehabilitation exercise on the occurrence of pneumonia in NSTE-ACS.Results The differences were statistically significant in factors including age,smoking,COPD,diabetes,left ventricular failure,renal failure,low serum albumin,early revascularization,and early rehabilitation exercise between two groups (P ＜ 0.05).By analysis of logistic,risk factors on the occurrence of pneumonia in NSTE-ACS were age,smoking,COPD,diabetes,left ventricular failure,renal failure,and low serum albumin(P ＜0.05).Early revascularization and early rehabilitation exercise were the protective factors to avoid pneumonia.Conclusions Left ventricular failure,age,smoking,COPD,diabetes,renal failure,and low serum albumin were the risk factors of pneumonia occurred in NSTE-ACS,but early revascularization and early rehabilitation exercise were the protective factors to avoid pneumonia.Actively treating the risk factors and early initiating the protective factors can effectively reduce the incidence of pneumonia.