Micrometastasis is an independable prognostic factor for the cancer patients.Furthermore,free-disease survival and overall survival of the cancer patients are correlated with micrometastasis.In addition, detection of micrometastasis can be used to monitor the efficacy of adjuvant therapy,predict outcome and im.prove the stage of patients with carcinoma.Micrometastasis has great significance for clinical comprehensivetreatment.The limitations of this study include the lackness of retrospective analysis,the small number of pa-tients studied,and the short follow-up time for the cancer patients.

Objective To investigate the therapeutic effect of transplantation of limbal epithelial cells and decellularized porcine cornea construct in rabbits with corneal alkali burn.Methods Allogeneic rabbit limbal epithelial cells were cultured and identified.Porcine cornea scaffold was fabricated by decellularization.The LECs-DPC construct was cultured and transplanted to the alkali-burned corneal surface of rabbits(4month follow-up).Results Immunocytochemistry and RT-PCR analyses revealed the presence of ABCG2 positive limbal stem cells.A general view showed the corneas were clear and vessel-free postoperatively.HE and Masson Trichrome staining results indicated the epithelial stratification,fewer cellular infiltration,DPC' integration into host corneal tissues,and more regular collagen configuration.Transmission electron microscopy exhibited formation of desmosomes and microvilli.Conclusion Allogeneic limbal epithelial cells transplantation using DPC as a carrier can restore corneal clarity after alkali burn in a rabbit model.

OBJECTIVE:To provide behavioral intervention strategies for the reduction of health risks involved in Chinese inhabitants' self-medication.METHODS:The health risks and the behavioral patterns involved in Chinese inhabitants' self-medication were analyzed.RESULTS &CONCLUSION:The self-medication behaviors of Chinese inhabitants can only be intervened through strengthening the health education,improving Chinese inhabitants' self-medication ability,standardizing the management of packaging and descriptions of OTC,promoting and enforcing GPP,launching consumer medication counseling services extensively,enhancing pharmacists' quality,reinforcing supervision and reporting of adverse drug reactions,etc.

Objective To investigate the effects of sevoflurane on the systemic inflammatory response and cardiopulmonary function in septic shock rats. Methods Thirty-two SD rats, 8-10 months old, weighing 250-300 g, were randomly divided into 4 groups (n = 8 each): sham operation group (group S), cecal ligation and puncture (CLP) induced septic shock group (group CLP) , sevoflurane I group (group SEV, ) and sevoflurane II group (group SEV,). The abdomen was opened but CLP was not performed in group S. The septic shock was induced by CLP as described by Baker et al. Group SEV, and SEV, inhaled 2.4% sevoflurane for 30 min at 1 h and 3 h after the successful establishment of the model respectively. At 1, 3 and 5 h after septic shock, MAP and HR were recorded and arterial blood samples were taken for blood gas analysis and determination of plasma concentrations of TNF-α, IL-1, MDA and NO. The left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular fractional shortening (LVFS) and cardiac output (CO) were also detected 5 h after septic shock. The animals were killed after the detection of cardiac function. The lungs were removed for determination of W/D lung weight ratio and Evans blue (EB) content. The tissues from the heart, lung, liver and kidney were taken for detection of NF-kB activity by electrophoretic mobility shift assay (EMSA) ResultsMAP was significantly lower, HR higher, LVEDD, LVESD, LVFS, CO, pH value, PaO2 and PaCO2 lower, and W/D lung weight ratio, EB content, plasma concentrations of TNF-α, IL-1, MDA and NO, and NF-kB activity in the heart, lung, liver and kidney tissues higher in group CLP, SEV, and SEV2 than in group S (P < 0.05). NF-kB activity in the heart, lung, liver and kidney tissues and plasma concentrations of TNF-α, IL-1, MDA and NO were significantly lower in group SEV, than in group CLP and SEV2 ( P < 0.05 ), but no significant differences were found in the other indices between group SEV, and CLP and between group SEV1 and SEV2 ( P > 0.05). Conclusion Inhalation of 2.4% sevoflurane for 30 min 1 h after septic shock can inhibit the systemic inflammatory response slightly, but can not improve the cardiopulmonary function in rats with CLP-induced septic shock.

Objective To study the effect of endoscopic treatment in acute severe pancreafitis. Methods EST(endoscopic sphincterepapiilotomy) and ENBD ( endoscopic naso-billary dralnage) or ENPD ( endoscopic naso-pancreatic drainage) were used to treat the acute severe pancreatitis(ASP). Results The recover time of blood dia-stase of the endoscopic therapy group and the contrast group was (3.2±1.5) days, (6.6±1.2) days, respectively (P<0.01) ; the incidence of needing to be operated was 0.7% (1/140), 6.5% (8/124), respectively (P<0.05) ;mortality rate was 0:7% (1/140) ,7. 3% (9/124) ,respectively(P <0. 01 ) ;the incidence of complication was 3.6% (5/140) ,41.9% (52/124), respecfivly(P<0.01). Conclusion EST + ENBD(or ENPD) in treating ASP has cer-tain effect and is the primary therapy to the ASP.

Objective To evaluate the clinical value of bronchoscopy lung biopsy Combined with brush cytology .Methods Collected 113 cases of diffuse or clinical cases of peripheral pulmonary disease examined by bronchoscopy lung biopsy Combined with brush cytology with no X-ray guiding during January 2011 to December 2012 .And the inspection results and the corresponding clini-cal data were retrospectively analyzed.Results In 113 cases 73 cases made a definite diagnosis, diagnostic positive rate was 64.60% in total, include 96.02% of diffuse patchy shadows, 72.13% of diffuse millet or nodular, 54.12% of local infiltration fo-cal, 32.11% of diffuse grid or ground glass honeycomb lesion, 28.26% of local tumor nodules.Conclusion There is significantly clinical diagnosis value of bronchoscopy lung biopsy Combined with brush cytology to examine diffuse and peripheral pulmonary dis-ease.

OBJECTIVE:To observe therapeutic efficacy and safety of low molecular heparin calcium (fraxiparine) combined with vapour-pressure type blood circulation driver for the prevention and treatment of postoperative lower limb deep vein thrombo-sis(DVT)in elderly fracture patients. METHODS:80 elderly patients receiving fracture surgery were randomly divided into con-trol group and observation group,with 40 cases in each group. Control group only received preventive measures for lower limb DVT under the action of vapour-pressure type blood circulation driver,while observation group was additionally given Low molecu-lar heparin calcium injection on the basis of control group,Preoperative 2 h,postoperative 12 h and 24 h,0.4 ml was administrat-ed;Postoperative d2-d3,0.4 ml was subcutaneously injected;postoperative d4,0.6 ml was administrated and lasted for 10 d. The incidence of lower limb venous thrombosis,therapeutic efficacy,the changes of blood rheology indexes and the cross-section diam-eter difference of uninjured side limb were observed in 2 groups;the incidence of ADR was compared between 2 groups. RE-SULTS:The observation group;total effective rate (95.00%) was higher than control group (72.50%),with statistical signifi-cance (P<0.05). After treatment,the cross-section diameter difference of side and uninjured side limb in observation group de-creased to(1.10±0.87)cm,which was significantly lower than control group(2.59±1.55)cm,with statistical significance(P<0.05). The blood viscosity at both high and low shear rates,plasma viscosity and fibrinogen of observation group were all better than those of control group,with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:In the prevention of lower limb DVT in elderly fracture patients,Low molecular heparin calcium injection combined with vapour-pres-sure type blood circulation driver show high effective rate,significantly improve blood rheological index and lower the incidence of lower limb DVT with good safety.

The International Society on Thrombosis and Haemostasis has proposed to hold World Thrombosis Day on October 13 annually from 2014 to improve awareness and education of thrombosis. Thrombotic diseases are multidisciplinary disorders involving multiple systems.Thus, multidisciplinary team treatment for thrombotic diseases should be developed in China.A comprehensive study of genetic factors for thrombotic diseases is expected to achieve early diagnosis and risk prediction for this type of disease, and thus precision diagnosis and individualized treatment can be achieved.

Medical consumables material is essential supplies to carry out medical work, which has a wide range of varieties and a large amount of usage. How to manage it feasibly and efficiently that has been a topic of concern to everyone. This article discussed about how to design a medical consumable material management information system that has a set of standardized processes, bring together medical supplies administrator, suppliers and clinical departments. Advanced management mode, enterprise resource planning (ERP) applied to the whole system design process.
Disposable Equipment ; Management Information Systems ; Materials Management, Hospital ; Software Design

Medical equipments are essential supplies to carry out medical work. How to ensure the safety and reliability of the medical equipments in diagnosis, and reduce procurement and maintenance costs is a topic of concern to everyone. In this paper, product lifecycle management (PLM) and enterprise resource planning (ERP) are cited to establish a lifecycle management information system. Through integrative and analysis of the various stages of the relevant data in life-cycle, it can ensure safety and reliability of medical equipments in the operation and provide the convincing data for meticulous management.
Equipment and Supplies ; Management Information Systems ; Reproducibility of Results