MicroRNAs (miRNAs) are a class of small,non-coding,single-stand RNA molecules which are composed of 21-25 ribonucleotides.They regulate gene expression at post-transcription and participate in many important biological processes including cell growth,apoptosis,viral infection and cancer development.At present,the studies on miRNAs in cervical lesion include miRNAs expression characteristic of cervical cancer and precancerous lesion,the association with HPV infection and prognosis of cervical lesion,cervical cancer development,diagnosis and therapy.

Objective To discuss effective nursing measures for patients with congenital hypospadia with the application of urethral stent.Methods 142 patients with congenital hypospadia were selected and used soft stent during and after operation.The urethral orifice was kept smooth after operation and the stent was removed until 2 weeks after operation.Results Two patients complicated with urethral fistula,one was because of the poor blood supply of the skin flap and the other because of the edema of the skin flap and the remove of stent.Other patients received good treatment effect. Conclusion Application of urethral stent in patients with congenital hypospadia could reduce the incidence of infection and increase the success rate of operation.

Objective To analyse the efficiency of postural control after 24h sleep deprivation.Methods To observe the change of the static posturography of 16 healthy male after 24h sleep deprivation.Results After 24h sleep deprivation,in both eyes-open condition,whole path length(0h:564.40±42.07,24h:535.23±33.28),unit-path-length(0h:6.96±3.20,24h:5.49±2.65),deflection average center displacement X (0h:7.01±2.79,24h:5.06±3.73)significantly decreased,but standard deviation Y(0h:3.17±0.83,24h:3.98±1.34)significantly increased.In both eyes-closed condition,whole path length(0h:645.70±80.05,24h:587.27±64.87)significantly decreased.Conclusion After 24h sleep deprivation,there are less spontaneous movements of body and lower efficiency of postural control.

Objective: To explore the characteristics of acculturation and its relationship to school life satisfac-tion for Hani junior and senior middle school students. Methods: The Acculturation Survey of Hani Middle School Students and School Life Satisfaction Rating Questionnaire for Adolescents were used to test 621 students in 3 senior middle schools and 2 junior middle schools from 3 different counties resided mainly by Hani people, in Honghe pre-fecture. Result: (1) The scores of acculturation and its dimensions had statistical significant difference in gender,family residence, and grade (F=3.46, 3.29, Ps<0.05). The total average scores of acculturation was more than median value for Hard students [(3.79±0.65) > median value 3] . The score of acculturation in female students was significantly higher than that in male students [(3.85±0.61)vs.(3.74±0.68), P<0.01] . The score of school educational identification in the students from rural areas was higher than that from town areas [(3.89±0.75)vs.(3.64±0.69), P<0.05]. Meanwhile, the score of Chinese familiarities in students from town was higher than that in ones from rural areas [(3.96±0.98)vs.(3.47±0.82), (3.53±0.90), P <0.05] . (2) As the graph of average data points were seen, with the transference of grades, the score of their ac-culturation appeared the winding changes. (3) The average scores of acculturation and its 3 dimensions were positively correlated with the school life satisfactions (r=0.52, 0.44, 0.51, 0.29, Ps<0.01). Multiple stepwise regression analysis showed that school life satisfaction was related to school educational identification and main-stream cultural identity. Conclusion: It indicates that female and ones lived in town have higher level of accultura-tion in Hani middle school students, and higher level of acculturation be correlated to higher level of school life sat-is faction.

Objective To determine the frequency of the cholesteryl ester transfer protein (CETP)-TaqIB polymorphism and investigate its relationship with plasma lipid levels and coronary hert disease(CHD). Methods Two hundred and thirty-eight patients with CHD (CHD group) and 203 age-matched controls( control group) were selected, the CETp-TaqIB mutation was detected by restriction fragment length polymorphism of the CETP gene. Results In the total subjects, the frequency of B1 and B2 alleles were 59.4%(262/441 ) and 40.6%( 179/441 ) respectively. Compared with that in control group, the frequency of CETP genotype BIBI was higher in CHD group [39.9%(95/238) vs 29.6% ( 60/203 ), P<0.05], and the frequency of B1B2 was lower in CHD group [44.1%(105/238) vs 53.7%(109/203), P< 0.05]. Compared with that in the B2 homozygotes, high density lipoprotein cholesterol (HDL-C) and apolipoprotein (apo)A I level were significantly lower in the B1 homozygotes [(1.19±0.36) mmol/L vs (1.38±0.39) retool/L,( 1.17±0.33 ) g/L vs ( 1.30±0.31 ) g/L, P<0.05]. The B 1 homozygotes was associated with higher degree of cononary stenosis than the B2 carriers (P<0.05 ). There was no significant association between CETP-TaqIB genotype and the risk of CHD (P=0.147). Conclusions CETP-TaqIB polymorphism affects the concentrations of lipaproteins. There are significant associations between the B1 homozygotes and lowerHDL-C and apo A I levels. The B1 allele is not an independent risk factor for CHD.

Reversible posterior leukoencephalopathy syndrome (RPLS) is a cliniconeuroradiological entity mainly characterized by the rapidly progressive increase in blood pressure, headache, vomiting, conscious disturbance and seizure. Neuroimaging showed symmetrical reversible white matter edema in bilateral cerebral hemispheres, particularly in posterior brain.Clinical symptoms and neuroimaging changes can be recovered completely with timely and correct treatment. The two major hypotheses about the pathogenesis of RPLS-cerebral vasospasm and cerebrovascular hyperperfusion-have been in dispute. At preseut, most researchers still agree that the latter is the main cause of cerebral edema. This article expounds the new viewpoints of its pathogenesis and the above two major hypotheses in recent years from the characterizations of etiology, pathology and imaging of RPLS.

BACKGROUND: The incidence of cognition disturbance after cerebral infarction is about 20% -30% and effective drugs for its prevention and treatment are anticipated.OBJECTIVE: To investigate the effect of nicergoline on cognition disturbance after cerebral infarction and explore its mechanism.DESIGN: Randomized controlled trial based on patients.SETTING: Neurological department in a medical university.PARTICIPANTS: A total of 60 patients admitted to the Neurological Department of the Second Hospital Affiliated to Chongqing Medical University for cerebral infarction during October 1999 and April 2001 were recruited in this study, and randomly divided into two groups, nicergoline treatment group and control group with 30 in each.METHODS: Mini-mental state examination(MMSE) score was evaluated and cerebral blood flow was determined with transcranial Doppler' s ultrasonography within one week after admission and three months after admission, respectively, and data were analyzed with SPSS software.MAIN OUTCOME MEASURES: MMSE score and velocity of blood flow in treatment and control groups.RESULTS: MMSE score in memory, calculation and recollection decreased significantly[ (1.2 ± 1.3), (2. 2 ± 2. 1) and(1.0 ± 1.7), respectively] in control group but did not change much[ (3.9 ± 1.4), (4. 4 ± 1.9) and(4.0 ± 1.6) ]in treatment group. The velocity of blood flow in control group decreased apparently, while it increased in treatment group[(58.31 ±10. 15) and(65.79 ±9.74) cm/s in the right middle cerebral artery].CONCLUSION: Nicergoline can prevent and treat vascular cognition disturbance, and improvement of blood supply may be one of the mechanisms.

Objective　To evaluate homology of 53 clinical str ains of M. chelonae abscessus subspecies which caused the nosocomial infecti on. Methods　Large restriction fragment (LRF) pattern analysis of genomic DNA by using pulsed-field gel electrophoresis was performed and differences of patterns were compared. Results　53 clinical strains had two types of LRF pat terns. 49 strains had the same one LRF pattern, the rest 4 had another pattern. Conclusions　The nosocomial postoperative M. Chelonae absc essus subspecies outbreak was mainly caused by the same infectious source. So pulsed-field gel electrophoresis was suitable for evaluating strain homolo gy and looking for infectious sourses.

Objective To evaluate the effect and safety of transobturator inside-out tension-free urethral suspension (TVT-O) as the surgical treatment on post-operational recurrence of female stress urinary incontinence (SUI). Methods Eleven women with SUI recurrence who underwent TVT-O were selected in the research. Their incontinence recurred from 1 months to 8 years after the previous surgical treatment.Results The operative time was (28 ±9) min, the bleeding volume was (15 ±5) ml. Postoperative complications: urinary retention in 1 case, vaginal erosion in 1 case. Follow-up was enforced for 6-36months, median time was 16 months. Grouts-Blaivas analogue scale:among the 11 patients, 9 patients were cured, 2 patients were improved. ICI-Q-SF score: preoperative 13-21 ( 15.36 ± 2.34) scores compared to postoperative 0-9 (1.46 ±3.27) scores (t =5.850,P ＜0.01 ). I-QOL score: preoperative 23.33-36.67(29.85 ± 4.74) scores compared to postoperative 66.67-100.00 (95.15 ± 10.12) scores (t = 17.753,P ＜0.01 ). PISQ-12 score of 10 patients: preoperative 16-25 (20.10 ± 3.07) scores compared to postoperative 11-20( 14.00 ± 2.83 ) scores (t = 11.600, P ＜ 0.01 ). Conclusion If conservative treatments fail, TVT-O is highly effective and safe for the postoperative recurrent SUI.

Vasculogenic mimicry (VM) is a term that describes a novel form of angiogenesis, channels of which are patterned networks of interconnected loops of periodic acid-Schiff (PAS)-positive extracellular matrix forming by highly aggressive tumor cells instead of endothelial cells. VM is closely correlated with the invasiveness, metastasis and prognosis of related tumors. However, traditional anti-vascular therapies aiming at endothelial cells have no remarkable effects on malignant tumor with VM. Therefore, VM should be an important factor to consider in design of anti-angiogenesis therapies of highly aggressive tumors and it is expected to become a potential target for tumor therapy. Some recent progresses of anti- vasculogenic mimicry therapy are reviewed in this article.