Objective To explore the pathologic changes of the opening blood-brain barrier(BBB) in the early stages of 50% TBSA scalded rabbits.Methods Ten male rabbits inflicted with 50% TBSA of Ⅲ degree were randomized into five groups: control,postburn 1 h,2 h,3 h,4 h.Brain tissues were isolated at different phases and observed in gross,by microscope and transmission electron microscope.Results Endothelial cells swelled and BBB began to open in 2 h after scald.The tight junction of endothelial cells became vague and the opening of BBB increased as lesion time prolonged.Conclusion As the lesion time prolonged,the injury of endothelial cells became more serious and the opening of BBB progressed,which resulted in the formation of brain edema.

To investigate the features of CT, ultrasonography and fundus fluorescein angiography (FFA) of morning glory syndrome, the data on CT, A/B-scan ultrasonography and fundus fluorescein angiography (FFA) were retrospectively analyzed in 8 cases of morning glory syndrome (MGS). Among those cases, 6 were examined with CT, 4 with FFA and 8 with A/B-scan ultrasonography. Results showed that the characteristics of CT, A/B-scan ultrasonography and FFA in MGS included: (1) The attachment spot of optic nerve became thin and vitreous body protruded to the posterior wall of eyeball with a spherical shape on CT image; (2) in the early period of FFA, hypofluorescence appeared on the optic, the abnormal arteriae and veins around the optic papilla were displayed clearly and in the late period, optic disc was stained with fluorescein; (3) on B-scan ultrasonogram, the vitreous cavity extended to the posterior pole and optic papilla, and projected to the basal part of muscle cones and thus the posterior part of vitreous cavity looked like an upside-down bottleneck. Sometimes the echogenic band of retinal detachment could also be seen. On A-scan ultrasonogram, both vitreous cavity and bottleneck showed no ultrasonic echoes and presented a base line without any evident wave crest. It is concluded that CT, A /B-scan ultrasonography and FFA could show the imageological features of MGS from different aspects, which helps clinicians to differentiate it from other diseases such as optic disc coloboma. CT and A /B-scan ultrasonography, in particular, are considered to be reliable imageological methods for the accurate diagnosis of MGS and are superior to the traditional techniques.

In recent years, there is increasing evidence that the pathogenesis of Parkinson's disease (PD) may involve the more generation of reactive oxygen species, and investigations on patients have shown that PD is under a status of oxidative stress. The defense against the toxic effects of reactive oxygen species is an essential task within the brain. An important component of the cellular detoxification of reactive oxygen species is the antioxidant glutathione. Consequently, it seems reasonable to propose that increase of brain concentrations of glutathione including glutathione analogs or precursors could be very effective in diminishing the cumulative effects of oxidative damage, and have been investigated as potential therapeutic targets in the treatment and prevention of PD or other neurodegenerative disease.

[Objective] To investigate the clinical efficacy of tacalcitol combined Halometasone sequential therapy in the patients of plaque psoriasis. [Methods] 117 cases of plaque psoriasis col ected between 2011.01 and 2013.02 were randomly divided into treatment group and control group. The treatment group were treated with tacalcitol and Halometasone therapy, and required topical use Halometasone(1 time/day) in the morning ,tacalcitol use in the evening(1 time/day) before the first 2 weeks. Continuous two weeks treatment from Monday to Friday with tacalcitol(2 times/day), Saturday and Sunday with Halometasone(2 times/day), final y the transition to be used alone tacalcitol(2 times/day) in the next two weeks of continuous treatment. Control group of pa-tients given alone Halometasone(2 times/day) for 6 consecutive weeks. Observe the adverse reactions PASI scores during and after completion of treatment within the two groups. Al patients were fol owed for three months, observed recurrence. [Results] The PASI score between two groups of patients declined during treatment, especial y at the 4th and 6th week within the treatment group. Both groups had no serious adverse reactions. In treatment group, 54 patients had effective responses, the total effective rate was 90%, significantly better than the control group(41 cases,71.9%). In the fol ow-up of 3 months, the recur-rence rate was 11.1%in the treatment group, significantly lower than the control group(31.6%). The difference of total efficiency and the recurrence rate with-in the two groups was statistical y significant. [Conclusion] Tacalcitol combined Halometasone sequential therapy of plaque psoriasis has significant clinical effi-cacy and low recurrence rate.

To investigate the effects of pyrrolidine dithiocarbamate (PDTC) on the proliferation and PCNA (proliferating cell nuclear antigen) expression of cultured human retinal pigment epithelium cells, human retinal pigment epithelium cells (RPE) were cultured from normal adults who, died accidentally. The effects of PDTC on the proliferation of RPE cells were examined by using methyl thiazlyl tetrazolium (MTT) assay. The effects of PDTC on the PCNA expression of RPE cells were immunohistochemically examined by employing biological image analysis system (BIAS). After treatment with PDTC of various of concentration ranging from 0.062 to 1 g/L for 24 h, or concentrations ranging from 0.031 to 1 g/L, the proliferation of RPE cells decreased in a dose-dependent manner. After treatment with PDTC of concentration varying from 0.062 to 1 g/L for 24 h, the PCNA expression was also suppressed in a dose-dependent manner. It is concluded that PDTC can inhibit the proliferation of RPE cells in vitro in a dose-and time-dependent manner, at least in part, by down-regulating the expression of PCNA. PDTC may be used to prevent and treat the proliferative vitreoretinopathy (PVR).

Objective To prepare the specific monoclonal antibodies(mAb) of Mycoplasma pneumonia(MP) and to establish a colloidal gold rapid detection method of MP by using mAb .Methods The nasopharyngeal swab samples were collected from chil‐dren patients with acute respiratory tract infection ,separated and cultured and performed the MP identification ,MP antigen was pu‐rified ,mAb of MP was prepared ,then the MP colloidal gold test strip was established by using MP mAb .The throat swab sample was collected from the patients with suspected MP infection and detected by using the fluorescent quantitative PCR and colloidal gold test strip .The detection results were observed and statistically analyzed .Results MP strain was successfully isolated and inac‐tivated ,the MP antigen was purified .The mouse was immunized by using this antigen ,19 strians of mAb against MP were prepared by using the hybridoma technique .Among them ,2 strains of mAb with high titer and good specificity(MP‐5 and MP‐19) were se‐lected as the raw materials for preparing the MP colloidal gold rapid test strip .The lowest detection limit was 20 ng .The clinical samples were detected by using the MP colloidal gold rapid test strip and fluorescent quantitative PCR .The results showed that the sensitivity of MP colloidal gold rapid test strip by using this established method was 88 .2% and its specificity was 82 .6% .Conclu‐sion specific mAbs against MP is prepared and the colloidal gold rapid detection method is preliminarily established which provides the help for rapid diagnosis in the patients with MP infection .

Objective To investigate the risk factors for postoperative anastomotic leakage in geriatric patients with esophageal carcinoma and provide new insights into clinical prevention and treatment.Method The fifty-six patients with anastomotic fistula were chosen from 636 esophageal carcinoma patients who underwent esophagectomy in Department of Thoracic Surgery from January 2011 to January 2016.In the 56 patients,the ratio of male (39 cases) to female (17 cases) was 2.3 ∶ 1.0,with the age range of 60-81 years,average of (66.4±8.6) years.The retrospective analysis was performed for obtaining the risk factors for anastomotic leakage.Results Fifty six cases who suffered from anastomotic fistula showed 10 cases (17.9％)with hormone administration,17 cases (30.4％) with preoperative chemo-radiotherapy,32 cases (57.1％) with the basic diseases,27 (48.2％) with preoperative dystrophia.In addition to the old age and tumor,the other risk factors for anastomotic leakage included the hormone use,preoperative malnutrition,and preoperative chemoradiotherapy.Conclusion High attentions have to be paid to the prevention and treatment of anastomotic fistula patients with chronic diseases,hormone use,preoperative malnutrition and preoperative chemo-radiotherapy.

Objective To investigate the pulmonary imaging findings and the clinical characteristics of ANCA-associated vasculitis.Methods 16 patients with ANCA-associated vasculitis confirmed by pathology in our hospital were analyzed retrospectively,all patients underwent 1 6-sclice high resolution chest CT scans.The clinical manifestations and lung CT findings of the patients were analyzed.Results The mean age of 1 6 patients (7 male and 9 female)was 62.7 (ranged from 56 to 83).The onset time of the disease was ranged for 1 weeks to 2 years,and it was characterized as multiple organs involvement accompanied by kidney damage with different degrees.The clinical respiratory symptoms inluded fever and cough.The lung HRCT features of ANCA-associated vasculitis were as follows:patchy shadow in 14 cases (87.5%),streak one in 12 (75%),nodular one in 3 (18.8%),cavities in 4 (25%),emphysema and pulmonary bullae in 6 (37.5%),tree-in-bud in 6 (37.5%),pleural effusion in 4 (25%),mediastinal lymph nodes enlargement in 3 (18.8%), pleural thickening in 6 (37.5%),cellular-like in 5 (31.3%),bronchiectasis in 6 (37.5%),round-glass opacity in 10 (62.5%)and reticulate changes in 8 (50%).Some patients were treated by the glucocorticoid (a hormone steroid)or by it in combination with immunosuppressant, and the clinical symptoms were improved and some lesions on X-ray images were significantly reduced or disappeared.Conclusion ANCA-associated vasculitis usually occurs in the majority of middle or old age without gender difference.Multiple organs are easily involved and the lesions in lung may be misdiagnosed as infection.Early CT diagnosis and timely treatment may improve the prognosis.

When a series of omics technologies such as genomics, epigenomics and proteomics are proposed, the concept of metabolomics occurs. Metabolomics is a top-down system biology approach, which analyzes endogenous metabolites by using high throughput, high resolution and high sensitivity based on metabolic analysis platform. By identifying characteristic biomarkers and analyzing the biomarkers of metabolic pathway, it will provide a new way for the diagnosis and treatment of diseases. Therefore, it is more and more widely used in bronchial asthma, chronic obstructive pulmonary disease, pulmonary cystic fibrosis, acute respiratory distress syndrome, pulmonary tuberculosis, lung cancer and other respiratory diseases. In this paper, the application of metabolome analysis in respiratory disease of recent years has been briefly reviewed.