Repeat prostate biopsy is of great significance for missed diagnosis of prostate cancer. We make a review on the independent risk factors, route selection and new techniques to improve clinicans’ cognition of prostate biopsy indications. MpMRI targeted biopsy is recommended for patients who are eligibile for repeat biopsy for higher detection rate of clinically significant prostate cancer and lower rate of non-clinically significant prostate cancer.

Objective To explore the efficacy of ZPLATE TM anterior fixation systems in the treatment of thoracolumbar fractures and dislocations.Methods 13 cases with old thoracolumbar fractures and dislocations accompanied with spinal cord injury have been treated by anterior decompression,implanting bone union and internal fixation with ZPLATE TM anterior fixation systems.Results After an average of 15 5 months followup,No plate and screw was loose and fragmentized,there is no pseudoarticulation formation,average union time are 3 7 months, and paraplegia showed different degrees of recovery.Conclusion It suggests that anterior approach provids thorough decompression and ZPLATE TM anterior fixation systems is a good spinal fixation system for the treatment of old thoracolumbar fractures and dislocations with the characteristics of strong fixation.

Objective To evaluate the different effects of the treatment of thoracolumbar fractures by pedicle of vertebral arch Tenor system and Harrington device.Methods The clinical data of 35 cases of thoracolumbar fractures were analyzed retrospectively, 23 of them were operated with Harrington device and 12 with Tenor system.Results These cases were followed up for 6 months to 2 years. The fractures treated with Tenor system got satisfied reduction, reached about 96 7% of normal vertebral height. The deformities were completely corrected and complications were significant lower than those derived from Harrington device treatment, and no delayed neurologic damage was found. Most cases got reduction with one to three Frankel grades of neurologic recovery.Conclusion The Tenor pedicle system is more effective in treatment of thoracolumbar fracture than non-pedicle device. It can get satisfied fracture reduction , reliable fixation and less complications.

Pseudomonas aeruginosa. Ziying granule can protect the mice infected by colibacillus and staphylococcus aureus and increase animal survival rate. Conclusion Ziying granule has broad-bacteriostasis effect on common pathogenic bacteria in pelvic cavity.

10.3969/j.issn.1000-8179.2013.12.016

ObjectiveTo evaluate the clinical effects of surgery using scar flap in situ combined with radiotherapy in 24 hours in the restorative treatment of keloids on the auricula and the preventive effects of the therapy in the recurrence of the keloids.MethodsThe scar flap in situ was designed,its size was large enough for covering the wound of the keloid on the auricula.The keloids along the designed lines were excised using local anesthesia,the flap was clipped into the one with even thickness and suitable size which covered the wound tensionlessly to ensure that the scar flap in situ survived well,and then the wound was bandaged with pressure and drained when necessary.18-24 hours after the surgery the wound was perpendicularly irradiated by the 5 MeV high energy electron beam (beta particle) of the Siemens Primus linear accelerator.After the dressing change was performed and the drain was removed; the wound was exposed to the irradiation,3-4 Gy segmentation dose per time,and the wound was then bandaged with pressure.The radiation was performed every two days and four times altogether with a total irradiation dose of 12-16 Gy.Stitches were removed 8-10 days after the surgery.ResultsThere were no avascular necrosis in the 25 scar flaps in situ and the wounds were all primary healing with normal color and fine appearance.All the patients were satisfied with the surgery.There was no recurrence of the 23 patients during the 8 to 42 months' follow-up,but there was a tendency to recur in 2 patients after 4-6 months,and the recurrence was controlled after the beta methasone was locally injected for 2-4 times.ConclusionsIt is not necessary to harvest the flaps on the other sites applying the sear flap in situ in the restorative treatment of keloids on the auricula,and therefore it prevents the formation of the keloids on the donor sites.Furthermore,the surgery is simple and the appearance of the auricula is fine,and it presents satisfactory clinical effects to irradiate the wound in 24 hours after the surgery.

Objective:To investigate the existence of side population cells with the potency of stem cells in human gallbladder carcinoma cell line GBC-SD and the differences in ABCG2,Oct-4 and CD34 expression among SP cells,non-SP cells and GBC-SD cells.Methods:SP and non-SP cells were sorted from GBC-SD cells by fluorescence-activated cell sorting(FACS).The expression of ABCG2,Oct-4 and CD34 in SP cells,non-SP cells,and GBC-SD cells was detected by reverse transcription-polymerase chain reaction(RT-PCR),Western blot,flow cytometry(FCM)and immunofluorescence chemistry.Results:SP cells with stem cell potency were isolated from GBC-SD cells with a proportion of(0.64±0.08)%.The metastatic ability of SP cells was obviously higher than that of non-SP cells and GBC-SD cells(P<0.05).The expression of ABCG2 was significantly higher in SP cells than in non-SP cells and GBC-SD cells[(89.56±3.86)%vs.(1.32±0.49)%and(12.37±1.61)%,P=0.001].The expression of Oct-4 in these cells was(94.87±1.40)%,(88.16±2.34)%and(90.17±1.61)%,respectively(P>0.05).CD34 was neady absent in these cells on protein level[(1.78±0.51)%vs.(0.63±0.21)%and(0.96±0.381)%,P>0.05)],but it was highly expressed in non-SP cells and GBC-SD cells and absent in SP cells off mRNA leve;.Conclusion:SP calls which hava the potency of stem cells,exist in human gallbladder carcinoma GBC-SD cell line and have the phenotype of ABCG2+Oct-4+CD34-.

The paper introduced the general picture of Xiangya Hospital in the past five years in its development of ahigh-level research hospital of international influence.Aiming at this strategic goal, the hospital proceeds by means of discipline development,talent training,research platform building, international academic exchange,performance appraisal and incentive,budget guarantee and system development.All these efforts contribute to the building of a mutual-supportive,efficient and sustainable medical science innovation system tailored to large hospitals.

[Abstract ] Objective At present, few studies are reported on procalciton (PCT) and endotoxin (ET) in the diagnosis of urosepsis after percutaneous nephrolithotomy ( PCNL) .The purpose of this study was to investigate the clinical value of the detection of serum procalcitonin and endotoxin in the early diagnosis of urosepsis after PCNL . Methods We retrospectively analyzed the clinical data about 427 cases of upper urinary tract stones treated by PCNL , among which urosepsis developed postoperatively in 49 ( the urosepsis group ) and the other 378 non-urosepsis cases served as controls .At 1 day and 2 hours before PCNL , we detected the levels of serum ET and PCT and analyzed the PCT and ET levels and the results of combined detection in the two groups of patients using the ROC curve. Results At 2 hours before surgery , both the levels of PCT and ET were significantly higher in the urosepsis group than in the non-urosepsis controls ([ 5.18 ±4.43 ] vs [ 1.38 ±1.01 ] ng/mL, P<0.01;[50.91 ±35.45] vs [17.86 ±10.78] pg/mL, P<0.01).ROC curve analyses manifested that the areas under the curve (AUC) of PCT and ET were 0.841 ±0.038 and 0.786 ± 0.043, their sensitivities were 79.6%and 71.4%, and their specificities were 78.0%and 70.1%, respectively.The combined de-tection showed the AUC, sensitivity, and specificity to be 0.915 ±0.029, 85.7%, and 86.5%, respectively, all significantly higher than either PCT or ET detection alone (P<0.01). Conclusion The combined detection of PCT and ET can significantly increase the sensitivity and specificity of early diagnosis of urosepsis after PCNL and is superior to either PCT or ET detection alone .

AIM: To investigate the expression of ICAM-1 mRNA in hepatic sinusoidal cells (HSCs) during liver ischemia-reperfusion injury and its effects. METHODS: Three groups of rabbit livers experiencing ischemia for 15, 30 and 60 minutes respectively, and reperfusion for 60 minutes were conducted. The hepatic ICAM-1 mRNA expression was observed and leukocytes in hepatic sinus were counted. RESULTS: The lower expression of ICAM-1 mRNA showed in HSCs before reperfusion. Besides, leukocyte counts could no change. However, the more expression of ICAM-1 mRNA was found at the point of 60 minutes reperfusion. Furthermore, the higher expression of ICAM-1 mRNA showed in the more prolonged ischemic liver. Meanwhile, there was significant positive correlation between ICAM-1 mRNA contents and leukocyte counts. CONCLUSION: The high ICAM-1 mRNA expression induced by hepatic ischemia in HSCs increased adherence of HSCs and sequestrated more leukocytes in liver.