Objective To study the significance of serum ischemia modified albumin(IMA) and brain natriuretic peptide(BNP) in patients with acute coronary syndrome. Methods Patients with acute coronary syndrome in six hours were divided into ST elevation myocardial infarction(STEM) of forty one cases, non-ST elevation myocardial infarction( NSTEM)of forty four cases and unstable angina of forty three cases. Twenty healthy people were selected as the control group. The serum IMA and BNP were detected. Results The levels of IMA and BNP in STEM group [(89. 6 ± 21.4) ABSU/ml, ( 324. 6 ± 39. 1 ) ng/L] were significantly higher than those in NSTEM group [( 79. 3 ±20. 5) ABSU/ml, (259. 7 ± 30. 5) ng/L]. The levels of IMA and BNP in NSTEM group were significantly higher than those in UA group[(67. 1 ± 18. 3)ABSU/ml,(124. 1 ±24. 3)ng/L].The levels of IMA and BNP in UA group were significantly higher than those in control group [( 56. 2 ± 15. 1 ) ABSU/ml, ( 15.9 ± 6. 7 ) ng/L]. Conclusion The levels of IMA and BNP in serum could forecast severity in patients with acute coronary syndrome.

According to clinical epidemiological researches, the usage of antibiotics in infant increases the risk of childhood asthma morbidity. The mechanism of this link is not entirely clariifed, and it is assumed of being related to side effects of antibiotics, which changes the colonization of normal intestinal lfora and reduces the diversity. It is clear that normal intestinal lfora of infant help to construct innate immune system and adaptive immune system, and forge immune tolerance. Therefore, normal intestinal lfora might be helpful in reducing allergies. The usage of antibiotics in early life changes the quantity and the composition of intestinal lfora, and breaks the balance of immune homeostasis, which might increase the risk of asthma morbidity. The abuse of antibiotics in children is a general phenomenon in China which needs attention from pediatricians.

BACKGROUND:The research for mesenchymal stem cels in the treatment of liver cirrhosis has made great progress. However,t-test analysis is often misused in clinical practice. OBJECTIVE:To analyze the efficacy and safety of umbilical cord mesenchymal stem cel therapy for liver cirrhosis by repeated measurement method. METHODS: A total of 27 patients with decompensated liver cirrhosis underwent conventional medical treatments, including liver protection treatment and symptomatic treatment. At 1 week after hospitalization, patients were given intravenous transplantation of umbilical cord mesenchymal stem cels (passages 2-4), cel viability≥ 90%, stem cel counting≥ 2×107 cels, four times with an interval of 5-7 days. Analysis of variance based on repeated measurement data was performed to analyze the liver function changes at different time after umbilical cord mesenchymal stem cel transplantation. RESULTS AND CONCLUSION: The results of univariate repeated measurement showed that at 2 and 3 months after treatment, the serum albumin level was increased and the total bilirubin level in serum was decreased significantly (P < 0.05); at 3 months after treatment, the content of aspartate aminotransferase decreased, and the content of cholinesterase increased significantly (P < 0.05). No patient appeared to have liver and other organ tumors during the observation period. These findings indicate that umbilical cord mesenchymal stem cel transplantation is effective and safe in the treatment of liver cirrhosis, which can significantly improve patient's liver functions.

Objective To investigate the selection and curative effect of surgical approach for thoracolumbar fractures. Methods A retrospective analysis was conducted on 212 patients with thoracolumbar fractures treated surgically from January 1995 to January 2009 to observe the spinal column and the neurological function recovery. Results Anterior surgery was performed in 73 patients and posterior surgery in 139. The patients were followed up for average 23.7 months ( range, 12-52 months). The height of injury vertebral, the Cobb' s angle and the volume of vertebral canal were improved significantly (P <0.01 ). The patients with incomplete paralysis all got nerve function recovery at different extents ( from one grade to three grades). Conclusions Surgical treatment for thoracolumbar fractures can effectively recover the spine structure and promote the nerve function recovery. The surgical approach should be selected based on the fracture type.

[ABSTRACT]AIM:TodeterminetheeffectsofShensongyangxincapsule(SSYX)ontheventricularelectrical properties, structural remodeling and cardiac function in the rats with diabetes mellitus (DM).METHODS:Male SD rats (n=45) were randomly divided into control group (n=15), DM group (n=15) and SSYX group (n=15).The rats in DM group and SSYX group were injected with streptozotocin (60 mg/kg, ip), while the rats in control group were given normal saline (1 mL/kg, ip).The blood samples were collected 72 h after treatment for determining the blood glucose lev-els in DM group and SSYX group .The model rats in SSYX group were administered with SSYX (1 g· kg-1 · d-1 , ig) for 6 weeks, while the other rats received normal saline (2 mL· kg-1· d-1, ig).The echocardiography was used to assess the cardiac function , and the lead II electrocardiogram was also recorded in all the animals .The radioimmunoassay and Masson trichrome staining were used to measure the plasma levels of endothelin -1 (ET-1) and the collagen deposition in the ventricles, respectively.A whole Langendorff-perfused heart model was used to conduct the electrophysiologic study .The monophasic action potential ( MAP) and the ventricular effective refractory period ( VERP) were recorded in the left anteri-or free wall ( LAF) , and the burst pacing was used to induce ventricular arrhythmia ( VA) .RESULTS: Compared with control group, the VERP, action potential duration (APD), QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were increased , while the cardiac function was declined in DM group .Compared with DM group , the VERP, APD, QT interval, incidence of VA, degree of myocardial fibrosis and plasma level of ET-1 were all de-creased, while the cardiac function was improved in SSYX group .CONCLUSION: SSYX attenuates the electrical and structural remodeling and improves the cardiac function in DM rats .

Objective To further analyze the chaos characteristics of heart rate variability(HRV) signals.Methods Analyzed HRV was obtained from 40 healthy subjects(20 olds,20 youngs) by means of Cao algorithm;Stability criteria was proposed to determine minimum embedding dimension;Meanwhile,we advanced a new quantative analysis method of studying HRV deterministic component,and applied the method to the old and young groups.Results Each subject's HRV was composed of both stochastic and deterministic components;Stability criteria could solve the subjective problem when determine minimum embedding dimension by Cao algorithm;Deterministic component in healthy old subjects was higher than the young.Conclusion In this paper,we have proved that HRV is deterministice chaos;Stability criteria works well for determining minimum embedding dimension;HRV decreases as age increases.

Objective To investigate the feasibility of minimally invasive muscle-sparing thoracotomy for gastric cardia tumor. Methods Minimally invasive muscle-sparing thoracotomy through the left 7th intercostal space was performed in 25 patients with gastric cardia tumor. Results Proximal gastrectomy and lower esophagectomy combined with esophagogastroanastomosis was performed in 20 patients with adenocarcinoma of gastric cardia and 4 patients with squamous cell carcinoma. Gastric fundus incision and tumor resection was conducted in 1 patient with hyperplastic polyps of gastric cardia. No perioperative mortality or serious complications were seen. Conclusions Minimally invasive muscle-sparing thoracotomy is simple and offers good exposure, being an alternative for carcinoma of gastric cardia in relatively early stages.

AIM: To explore if metabotropic glutamate receptors (mGluRs) i nduces neuroprotection against 6-hydroxydopamine (6-OHDA) neurotoxicity in PC1 2 cells. METHODS: The alteration of the glutamate in extracellul ar fluid of PC12 cells was detected by high performance liquid chromatography (H PLC) with a fluorescent detector. The cytotoxic activity of PC12 cells was assay ed by means of MTT colorimetric method. RESULTS: 6-OHDA dose-d ependently increased glutamate release and decreased cell activity of PC12 cells , and the mGluRs ligands had no significant effect on that. CONCLUSION: The mGluR3 ligands has no protective effect on neurotoxicity of PC12 cel ls induced by 6-OHDA.

Objective To evaluate the operative treatment of pediatric ureteropelvic junction (UPJ)obstruction. Methods A total of 34 cases of UPJ obstruction in children had double J tubes placed intraoperatively during Anderson Hynes pyeloplasty.Urethral catheterization was maintained for 5～7 days,the double J tube being removed on cystoscopy 4～6 weeks after operation.The outcome was evaluated with ultrasonography or intravenous pelviureterography 3～6 months postoperatively. Results The obstruction was successfully relieved in all the patients and no complications such as urinary infection and leakage of urine were noted on follow up. Conclusions Anderson Hynes pyeloplasty is an effective procedure for the treatment of pediatric UPJ obstruction. The routine use of double J tubes placed intraoperatively can reduce the chance of UPJ restricture and shorten the hospital stay.It is safe and dependable.