Kawasaki disease can affect the coronary arteries,including coronary artery dilation,aneurysm,stenosis and thrombus.Conventional coronary angiography is the gold standard for coronary artery evaluation,but there are risks associated with its invasive nature and with the exposure to contrast agents and radiation.With the rapid development,computed tomography angiography and magnetic resonance angiography become the noninvasive imaging modalities to evaluate the coronary artery.

Objective To investigate the attitudes of medical students regarding to organ donation compensation.Method A self administered questionnaire was distributed to 600 medical students selected randomly from two medical universities in China between January 2013 and January 2014.The questionnaire included demographics and core questions related to medical student's attitudes regarding organ donation compensation.Result A total of 600 questionnaires were distributed and 592 valid responses were received,including 150 doctoral students,143 master students and 299 undergraduate students.In all,69.8％ of medical students believed that compensation for organ donor was essential.Nearly 60.5 ％ believed financial compensation would increase the rate of organ donation but would not tarnish the behavior of organ donors (64.7％).At the same time,over 70％ of medical students believed that financial compensation would increase the number of living organ donation.With regarding to the ways of compensation,most of the students (80.4％) were in favor of priority on waiting list.Reducing or free of hospitalization costs (50.4％) and increasing the reimbursement ratio of medical insurance (41.8％) were also the acceptable compensation ways to medical students.Conclusion Most of medical students believe that it is essential to give compensation to organ donors.Enjoying the priority on waiting list,reducing or free of hospitalization costs and increasing the reimbursement ratio of medical insurance are the reasonable incentive methods.

Objective To translate the English version of Control Attitudes Scale-Revised (CAS-R) into Chinese and to test the reliability and validity of the Chinese version of CAS-R in patients with heart disease.Methods Totally 125 patients with heart disease were recruited and were investigated by the Chinese version of CAS-R.Results The internal consistency coefficient of the Chinese version of CAS-R ranged from 0.735 to 0.862.Factor analysis got three factors,which explained 54.128％ of the total variance.Using BDI as an external criterion,the correlation coefficients of the CAS-R and BDI scores were-0.59.Pearson coefficient with three dimensions of BDI were-0.48、-0.51 and 0.42.Conclusions The Chinese version of CAS-R has been proved to be reliable and valid.It can be used as a valid tool for the measurement of perceived control in patients with heart disease.

Objective:To determine blood magnesium and urinary magnesium in children with asthma exacerbation and discuss the relationship between the magnesium level and children with asthma attack. Methods:The observation group include 90 children who were 0~6 years old with asthma exacerbation that admission to our hospital since May 2008 to May 2012. The control group was 100 children with the same age to the observation group to accept the health examination. Every child in two groups were taking venous blood of 2ml and 24h urine samples. Record the amount of urine at the same time. Using automatic biochemical analyzer to detect the serum magnesium concentration and urinary magnesium concentration. Results: The urinary magnesium concentration of observation group was obviously lower than control group (t=8.52, P<0.001);the serum magnesium concentration of two groups had no significant difference (t=2.21, P>0.05). Conclusion:Children with asthma in acute attack stage with magnesium deficiency. Urinary magnesium concentration determination is simple, and it is a effective method that can be used to judge whether there is magnesium deficiency. Serum magnesium concentration cannot be used as a reliable indicator of magnesium ion balance.

Background and purpose:Backgrounds and Objective: Oxaliplatin is a third-generation platinum compound,there are some distinct differeuces in mechanism between cisplatin and oxaliplatin,and oxaliplatin is less toxic Poorly differentiated cartinoma is the most common histological type,for nasopharyngeal carcinoma,although radiotherapy is the basic therapeutic approach to nasopharyngeal carcinoma,chemotherapy is also important for recurrent and metastatic nasopharyngeal carcinoma,so in this work we analyzed the effects of oxaliplatin on nasopharyngeal carcinoma cell lines in vitro.Methods:CNE2 was incubated with oxaliplatin at various concentrations and times,cell growth inhibition was assessed by MTT-microculture tetrazolium assay,cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopy transmission electron.Results:Growth of CNE2 cells was significantly inhibited in a dose-dependent and time-dependment fashion.The inhibition of cell growth inhibition was(95.6?0.7)% after incubation with 100 ug/ml oxaliplatin for 48 hours,the cell was arrested at G_(2)/M and apoptosis was induced.When CNE2 cells were treated with oxaliplatin at the concentration of 0、0.03、4.0、100 ?g/ml,the rates of CNE2 cell apoptosis were(0.19?0.17)% 、(0.37?0.09)%、(5.50?1.08)%、(9.43?0.09)%,respectively.24 hours after 20 ?g/ml the CNE2 cells were characterized by chromatin condensation,chromatin crescent formation,nucleus fragmentation and apoptosis body by transmission electron microscopy.Conclusions:Oxaliplatin suppresses the growth of CNE2 cells in vitro by causing cell-cycle arrest and cell apoptosis.

Lipoxygenase(LOX) is related to emergence and development of many diseases, such as acute and chronic inflammation, atherosclerosis, hypertension and tumor. Therefore, the inhibition of LOX may play an important role in the prevention and treatment of these diseases. Many kinds of flavonoids, such as chalcones, flavonols, flavones and flavanols, have inhibitory effect on 5-LOX, 12-LOX and 15-LOX probably through inhibiting expression of LOX, bonding to the enzyme or reacting with free radicals generated at the active site of the enzyme. Their inhibitory activities are related to their structures. The inhibitory effects of flavonoids on LOX maybe one of mechanisms of flavonoids' some biological functions, such as anti-inflammatory, anti-tumor and reducing the risk of cardiovascular disease and cerebrovascular disease.

Objective Discussing the rationality of psychological intervening therapy in the treatment of early breast neoplasm. Methods By summarizing and reviewing 84 patients suffering from the early breast cancer between January of 2003 and December of 2005, we procured some information of their clinical manifes-tations, clinical therapy and statistic results of psychological assessment. Results By intervening the psycho-logical therapy, the patients' self-efficiency and living quality can be bettered. Conclusion Patients with early breast neoplasm need to have a comprehensive treatment with general or local therapy and individual psychologi-cal intervention. Nevertheless, it is rather practical to enhance the general level of prevention and therapy of breast neoplasm through the research of psychological problems after the operation.

Objective To explore the effect of c fos antisense gene transfection on the protection of cardiomyocytes following hypoxia and burn serum treatment. Methods Burn serum was collected from Wistar rats with 30% total body surface area(TBSA) of Ⅲ degree burns. The mixture gas containing 1% O 2 was used as hypoxia model. The c fos antisense gene recombinant was constructed by genetic recombination technique. Cardiomyocytes from neonatal Wistar rats were cultured in vitro with hypoxia and burn serum treatment. c fos antisense gene recombinant was transfected into the cultured cardiomyocytes. Expression of c fos mRNA was determined by RT PCR. Expressions of c fos protein, troponin T and ? Tubulin in cardiomyocytes were determined by Western blotting in the transfected and non transfected groups. Results RT PCR results showed that the expression of c fos mRNA increased significantly in the non transfected group. But after transfection of c fos antisense gene recombinant, the expression of c fos mRNA decreased significantly as compared with the non transfected cardiomyocytes. Western blotting results showed that the expression of c fos protein in the transfected group decreased remarkably as compared with the non transfected group, but the expressions of ? Tubulin and troponin T increased significantly in the transfected group. Conclusion Burn serum and hypoxia can cause the injury of cardiomyocytes. c fos antisense gene recombinant transfection has the protective effect on cardiomyocytes exposed to burn serum and hypoxia.

Objective To explore the relation of c jun antisense gene transfection and cardiomyocyte apoptosis following hypoxia and burn serum treatment Methods Burn serum was collected from Wistar rats with 30% total body surface area(TBSA) Ⅲ degree burn Rats inhaling mixed gas containing 1% O 2 was used as hypoxia model The c jun antisense gene recombinant was constructed by genetic recombination technique Cardiomyocytes from neonatal Wistar rats were cultured in vitro with hypoxia and burn serum treatment c jun antisense gene recombinant was transfected into the cultured cardiomyocytes Cardiomyocytes were stained with TUNEL for the examination of cardiomyocyte apoptosis at 12, 24 and 48 h after hypoxia and burn serum treatment In addition, the number of apoptotic cardiomyocytes was counted The results were processed statistically Results In the group with only the addition of burn serum and hypoxia(non transfected group), the numbers of apoptotic cardiomyocytes(mean per high power visual field) were 7 1?0 842, 28 4?1 635 and 13 2?1 525, respectively But after transfection of c jun antisense gene recombinant, numbers of apoptotic cardiomyocytes were 4 1?0 716,12 3?1 455 and 8 5?1 341, respectively There was a significant difference between the transfected group and the non transfected group( P

OBJECTIVE To understand the distribution and antibiotic resistance of the pathogens causing urinary infection among the patients with urinary calculus.METHODS Totally 406 strains were isolated from the 1244 urinary specimens.The antibiotic susceptibility of these strains was detected.RESULTS The most of pathogens were G-bacteria(348,85.7%).G+ bacteria and fungi accounted for 12.1%(49/406),and(2.2%,9/406).The most popular pathogens were Escherichia coli(54.9%),Klebsiella pneumoniae(8.1%),Staphylococcus(7.4%),Enterobacter(4.9%),and Enterococcus(3.7%).The detection rate of ESBLs was 52.5%.Meticillin-resistant Staphylococus accounted for 83.3%.Clindamycin-induced resistance of Staphloccocus accounted for 20.0%.6.7% Vancomycin-resistant Enterococcus were isolated.CONCLUSIONS G-bacteria are the most popular pathogens and show increased resistance to common antibiotics.The results of the paper will be usefal for the doctors to choose antibiotics reasonably.