With the population aging, diet changing and incveasing risk factors on vascular disease, the lower extremity ischemic disease has become a frequently occurring disease of older person, and it is the main reason for amputation disability. In the 21st century the stem cells transplantation is one of the most advanced technologies and has been applied quickly to clinical therapy, regarded as a radical treatment of lower extremity ischemic disease. Endothelial progenitor cells have gradually become a new direction and a new research focus because of its unique biological characteristics in the treatment of this disease. This article focuses on endothelial progenitor cells in treating extremity ischemia lesions on the theoretical basis and research developments.

Objective To observe the clinical effect of Angelica injection combine with psychological intervention in treatment of patients with lower limb fracture postoperative analgesia.Methods 60 cases underwent lower limb fracture surgery from February 2013 to April 2015, and were randomly divided into experimental group and control group, 30 cases in each group.Control group were treated with conventional medicine epidural injection morphine for postoperative analgesia, experimental groups were injected with Angelica injection treatment in control group on the basis of conventional therapy, recorded and analyzed two groups of patients improved postoperative pain scores, postoperative stress and pain after treatment. Results After treatment, 4, 8, 24, 48h pain scores of experimental group were lower than of control group (P<0.05); After 4h, 48h post-operation heart rate and mean arterial blood pressure was better than of control group (P<0.05).Conclusion Effect of conventional western medicine combined with Angelica injection postoperative analgesia is superior to conventional medicine alone , with no obvious side effects.

Concussion injuries of peripheral nerves are frequently encountered in gunshot wounds of extremities. With the aid of high speed X-ray imaging technic, the sciatic nerves of dogs were visualized continuously during the formation of the temporary cavitation when a high-velocity of bullet was shot into the thigh.It was concluded that the concussional injury of the nerve was the result of a combination of stretching, compression, and concussion. There was a exponential correlation among cavity volume, absorption of energy and nerve displacement. The pathological changes included extensive microvascular injury and partial breakage of nerve fibres. Extensiveness and lack of uniformity in pathological changes may have some clinical implications.

Objective To evaluate the effectiveness of wireless capsule endoscopies in patients with suspected CD of the small bowel.Methods From May 2002 through April 2003, we prospectively examined 20 suspected CD patients by capsule endoscopies with normal results in other conventional examinations. It includes the presence of the following symptoms and signs: abdominal pain, weight loss, positive fecal occult blood test, iron deficiency anemia, diarrhea and fever. Mean duration of symptoms before diagnosis was 6 5 years.Results Of the 20 participants, 13 (65%) were diagnosed as having CD of the small bowel according to the findings of the M2A Given Capsule. Among the findings detected by the capsule were mucosal erosions (2 patients), aphthae (5 patients), granulomatous nodularity (1 patient), large ulcers (2 patients), and ulceration with incomplete obstruction (3 patients). All of the 13 patients who received medications showed good clinical improvement.Conclusion Wireless capsule endoscopies were the effective device for diagnosing patients with suspected CD, undetected by conventional diagnostic measures.It may be more likely to detect early lesions in the small bowel of patients with CD.

Objective After partial hepatectomy, hepatopoietin(HPO) increase rapidly. The present study was designed to investigate how rhHPO and partial hepatectomy induce the expression of immediate early gene. Methods The Wister rats were divided into hepatectomy group and control group. The different gene expression within 1 h after 2/3 partial hepatectomy and in primary cultured hepatocytes system were examined by using representational difference analysis. Results In the expressed sequence tag(EST) library, most of these genes were identified as immediate early genes, and one new gene Tec that might be associated with liver regeneration in the EST library was found. Moreover, the Tec gene was rapidly induced by 2/3 partial hepatectomy. The expression peak achieved within 1 2 h after operation. HPO can rapidly induce the expression of these genes(c fos, LRF 1 and Tec, etc.) in primarily cultured rat hepatocytes. Conclusion rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. This is the first report that Tec gene is an immediate early gene related to liver regeneration.

Objective To assess the changes of coagulation and fibinolysis in patients undergoing thoracotomy. Methods Forty patients undergoing thoracotomy were enrolled into this trial. The venous blood were collected for normal coagulable function test at the following time points: before surgery, at 3 h after the beginning of surgery, at 1 d and 3 d after surgery. Results PT, APTT levels had no obvious change. Compared with before and during surgery, FG levels decreased obviously at 1 d and 3 d after surgery. TEG showed the value of R and K increased during and after surgery and value of MA decreased during and 1 d after surgery, but they had no significance in statistics. CI decreased significantly during and after surgery as compared with before surgery(P