Acupuncture Therapy ; instrumentation ; methods ; Aged ; Female ; Humans ; Male ; Middle Aged ; Periarthritis ; physiopathology ; therapy ; Range of Motion, Articular ; Shoulder Joint ; physiopathology ; Treatment Outcome

Objective To study the effect of Nurrl gene on the differentiation of rats' marrow stromal cells(MSCs) into neurous under the co-inducement of total panax notoginserg saponins(tPNS) and all-trans-retineic acid(ATRA) by coloning the Nurrl gene and transfecting it into MSCs. Methods Expressing plasmids pcDNA3.1-hygro-Nurrl were cloned,then transfected into MSCs with lipofectamine 2000.To begin with,MSCs were subcultured into 6-wells cultured plate at about 5?10~5 cells/well density and the wells were divided into four groups randomly which were Nurrl+tPNS/ATRA group,tPNS/ATRA group,Nurrl group and control group.Secondly,the plasmids were introduced to the MSCs in Nurrl+tPNS/ATRA group and Nurrl group,then protein expression of Nurrl was identified with immunocytochemistry.Thirdly,after the MSCs and plasmids had been co-cultured for 48 hours,cells in Nurrl+tPNS/ATRA group and tPNS/ATRA group were induced with BME in advance then with tPNS/ATRA in due form.For cells in Nurrl and control group,the only difference was that tPNS/ATRA was replaced with the culture.Finally we compared the different percentage of positive cells in four groups with TH,AChE and GABA antibodies by immunocytochemistry method. Results The immunocytochemical test showed that the MSCs transfected with Nurrl gene expressed Nurrl protein.The percentage of positive cells of TH antibody in Nurrl+tPNS/ATRA group was(38.4?4.6)% distinctly higher than that of tPNS/ATRA group,which was(5.9?3.4)%.Conclusion With tPNS/ATRA induced and immunocytochemistry of TH,positive cells percentage in Nurrl+tPNS/ATRA group was higher than that in tPNS/ATRA group,which showed a statistic difference.And the inducing function of tPNS and ATRA in MSCs differentiating into neurons was definite.

With the illustration of the L-Arg-NO-cGMP pathway in penile erection,the researches on nitric oxide synthases(NOSs),which produce nitric oxide(NO)as the main messenger to control the process,has stayed in the spotlight.nNOS locates in neuroal tissues and eNOS is binding on the membrane of cavernous sinus endothelium with caveolin.The nNOS & eNOS are expressed constitutively.The activation of nNOS & eNOS is more dependent on Ca2+ concentration than iNOS,which is seldom expressed except in aged or pathological conditions.It is now clear that nNOS may initiate cavernosal tissue relaxation under the regulation of PDZ zone when transcripted,while phosphorylated eNOS may facilitate attainment and maintenance of full erection.iNOS expression stimulated by the cytokines may be beneficial(cytoprotective or anti-fibrotic)or deleterious(cytotoxic)according to the tissue environment.Gene therapies based on NOSs have attained a great deal of achievements in animal experiments and will promise a wonderful future.

Objective To investigate the effect of tonic pain stimulation on the attention network test (ANT) . Methods Twenty healthy males recruited from university were enrolled in this study, and laboratory pain was induced by cuff pressure stimulation on the upper arm. All subjects performed the task separately under pain, no-pain and pressure conditions. The reaction time and accuracy rate of the ANT were quantified. The subjective pain perception was assessed by the short-form McGill Pain Questionnaire ( SF-MPQ) . Results Across three conditions, the executive network showed significant pain effect (F = 76.31 , P< 0.001) : no-pain condition at (92.51 ±35.71 )ms, pressure condition at (94.06 ±25. 28) ms, pain condition at ( 120.24 ± 18.21 )ms. Furthermore, the average reaction time and the A-scores (i. e. affective words) in SF-MPQ caused by pain was positively correlated ( r = 0.11, P = 0.03) . Conclusion Pain can result in deficit in the executive network for attention regulation. The negative affections caused by pain can interfere with attention.

Objective To evaluate the effect and safety of transobturator inside-out tension-free urethral suspension (TVT-O) as the surgical treatment on post-operational recurrence of female stress urinary incontinence (SUI). Methods Eleven women with SUI recurrence who underwent TVT-O were selected in the research. Their incontinence recurred from 1 months to 8 years after the previous surgical treatment.Results The operative time was (28 ±9) min, the bleeding volume was (15 ±5) ml. Postoperative complications: urinary retention in 1 case, vaginal erosion in 1 case. Follow-up was enforced for 6-36months, median time was 16 months. Grouts-Blaivas analogue scale:among the 11 patients, 9 patients were cured, 2 patients were improved. ICI-Q-SF score: preoperative 13-21 ( 15.36 ± 2.34) scores compared to postoperative 0-9 (1.46 ±3.27) scores (t =5.850,P ＜0.01 ). I-QOL score: preoperative 23.33-36.67(29.85 ± 4.74) scores compared to postoperative 66.67-100.00 (95.15 ± 10.12) scores (t = 17.753,P ＜0.01 ). PISQ-12 score of 10 patients: preoperative 16-25 (20.10 ± 3.07) scores compared to postoperative 11-20( 14.00 ± 2.83 ) scores (t = 11.600, P ＜ 0.01 ). Conclusion If conservative treatments fail, TVT-O is highly effective and safe for the postoperative recurrent SUI.

SUMO (small ubiquitin-related modifier) is involved in the post-translational modifications of proteins,and this process is referred to as SUMOylation.SUMOylation plays an important role in the regulation of cellular activities such as strengthening the stability of the protein,nucleocytoplasmic transport,DNA repair,DNA replication,mitotic and meiotic chromosome behavior,et al.SUMOylation is a dynamic process and can be reversed by SUMO-specific proteases (SENP).Once the balance between SUMOylation and de-SUMOylation is broken,there will be an aberrant expression of SUMO or SENPs in cells to happen,which may lead to the tumor occturtrence.

AIM: To study the effects of oxidized high-density lipoprotein (oxHDL) on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) and intracellular free calcium concentration （［Ca2＋］i） level in cultured human umbilical venous endothelial cells(HUVECs). METHODS: The MCP-1 protein content in the medium of conditioned HUVEC was measured by ELISA, and the ICAM-1 on HUVECs was detected by indirect immunofluorescence, and ［Ca2＋］i was determined by Fluo-3/AM, the injury of cells was observed by scanning electron microscopy (SEM).RESULTS: oxHDL could induce the expression of MCP-1 and ICAM-1 in HUVECs. In oxHDL group (HUVECs were incubated with 100 mg protein/L oxHDL for 24 h), the levels of MCP-1, ICAM-1 and ［Ca2＋］i increased by 160%, 60% and 70% respectively compared with the control group (P＜0.01). When HUVECs were incubated with 300 mg protein/L oxHDL for 24 h, cells were injured obviously. CONCLUSION: By inducing the expression of ICAM-1 and MCP-1 in endothelial cells, oxHDL may promote monocyte-endothelium adhesion and monocyte migration to intima, it may promote atherosclerosis as oxidized low-density lipoprotein (oxLDL).

[Summary] Long noncoding RNA( lncRNA) plays an important role in regulating the life activities, and its disordered expression is closely related to many diseases. Several studies have shown that lncRNA could modulate the endocrine metabolic processes at transcription, post-transcription, and the epigenetic modification levels, but the exact mechanism is still unclear. Further research needs to be done. This review will briefly describe the research progress of lncRNAs in endocrine and metabolic respects.

OBJECTIVE:To discuss the effects of Rujieping capsule on estrogen receptor(ER) and progesteron receptor(PR) of pathological tissues in rats with hyperplasia of mammary glands.METHODS:The rats were injected i.m with Estradiol benzoate injection for 25 days followed by the injection of Progesterone injection i.m for 5 days to establish the model of hyperplasia of mammary glands.Which were treated with high and low doses of Rujieping capsule respectively for 30 days with tamoxifen as positive control.RESULTS:Both tamoxifen and high and low doses of Rujieping capsules could lessen the symptoms of red swelling of nipples or hyperplasia of mammary glands,lower the levels of E2 and PRL,enhance the level of progestone,lower the index of uterus,markedly reduce the number of layers of the ductal epithelial cells,reduce the levels of ER and PR in mammary glands,and suppress mammoplasia.CONCLUSION:Rujieping capsules is effective in the treatment of hyperplasia of mammary glands in rats by regulating the level of estrogen and improve pathomorphologic change of hyperplastic mammary glands.

OBJECTIVE:To study the immunoregularoty effect of Lappaconitine in S180 tumor-bearing mice.METHODS: Each mouse was inoculated hypodermically with S180 tumor cells and normal sodium suspension,24 hours later,all the mice were injected i.m.with Lappaconitine q.d for 10 consecutive days.The immune function of the S180 tumor-bearing mice was detected.RESULTS: In Lappaconitine-treated mice,serum IgG level was heightened significantly;the 2,4-DNFB-induced delayed type hypersensitivity was enhanced;the induction of transformation of lymphocytes into lymphoblast was achieved;the phagocytic function of reticuloendothelial system was enhanced significantly,and the immune function of red cells was promoted.CONCLUSION: Lappaconitine could inhibit the growth of S180 tumor cells and significantly enhance the cellular immune function of S180 tumor-bearing mice.