Objective To investigate the characteristics of clinical application of skin flap supplied by sural nerve nutrition blood vessel. Methods The flaps were used in 17 cases to repair the soft tissue defect in heel,mallelous,anterior tibial and achilles tendon. Results 14 cases of 17 survived satisfactory. 2 cases presented edema and partially necrosis,they finally survived after open decompression and change of dressing. 1 case presented chronic sinus because of osteomyelitis of calcaneus. 6 months later, when the fracture healed, we performed debridement, removal of internal fixation, curettage and irrigation. It finally healed. Conclusion The sural nerve nutrition blood vessel flap was easy to elevate and safe, which was an ideal flap for repairing the soft tissue defect around the heel, malleolus, achilles tendon and the anterior tibial.

Objective To observe the effects of red blood cell lysis buffer on the isolation and culture of human bone marrow mesenchymal stem cells (hMSCs) in vitro.Methods Twenty-two bone marrow samples were randomly divided into 2 groups,including 11 samples of crushed red blood cell prepared with Tris-NH4Cl red blood cell lysis agent and 11 samples prepared without red blood cell lysis agent.The intervals from primary generation to 1,2 and 3 passages and the time of expansion to l07 cells were compared between the two groups.Results The times for P1,P2 and P3 passage was ( 9.3 ± 4.9 ) days vs.( 7.2 ± 1.0 ) days,( 14.4 ±4.7) days vs.( 14.5 ± 3.5 ) days,and ( 18.5 ± 5.0 ) days vs,( 20.1 ± 4.4 ) days,respectively,in crushed red blood cell group and non-crushed red blood cell group.The differences were not significant ( t =1.39,t =0.06,t =0.80,P ＞ O.05 ).The time for expansion to 107 cells in two groups was not significantly different ( t =0.80,P ＞0.05).Conclusion Tris-NH4Cl agent in red blood cell lysis has no significant effects on hMSCs isolation,culture and cell proliferation,which indicates erythrocyte lysing may be not an independent step for hMSCs isolation and expansion in vitro.