1.The safety situation of the digital hospital and its countermeasures.
Min WANG ; Yi GE ; Hou-bing DENG ; Xiang-jun LI
Chinese Journal of Medical Instrumentation 2006;30(3):213-214
This article introduces briefly the content of the disaster backup technology which should be attached same great importance to, as the construction and application of a information system.
Automatic Data Processing
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instrumentation
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Computer Security
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Hospital Information Systems
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organization & administration
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Humans
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Risk Adjustment
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methods
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Security Measures
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Software
2.Anatomic investigation of the pedicle fat grafts with the third lumbar segmental artery and its application in reoperation for lumbar disc herniation.
Chun-Zhen WANG ; Deng-Lu LI ; Shi-Xiang MU ; Bing-Zhu HOU ; Xin LIU
China Journal of Orthopaedics and Traumatology 2014;27(5):401-404
OBJECTIVETo investigate the blood supply of the pedicle fat grafts with the third lumbar segmental artery and its clinical effects on reoperation for lumbar disc herniation.
METHODSTwelve sides of 6 adult cadaver examples were contributed to investigate the courser of lumbar segmental vessels and the distribution of hypodermic capillary net of the dorsal branch of the third lumbar segmental artery. From January 2000 to January 2007,49 patients needed reoperation to treat lumbar disc herniation,including 26 males and 23 females with an average age of 55.6 years (ranged from 39 to 70 years). Duration between two operations ranged from 8 months to 15 years with an average of 6.9 years. Reoperative reasons included recurrent lumbar disc protrusion(30 cases)postoperative epidural scar formation (17 cases), postoperative epidural cyst formation (2 cases). Of them,9 patients underwent posterior lumbar interbody fusion at the second operation. The pedicle fat grafts with the third lumbar segmental artery were covered on the sites of the laminectomy in these patients. After negative pressure drainage tube were pulled out, 2 ml Chitsan were injected to the sites of the laminectomy and around epidural nerve root through epidural catheter. VAS score and the Oswestry Disability Index (ODI) were used to assess clinical outcomes before and after operation.
RESULTSThe courser of third lumbar segmental vessels were invariant at the lateral face of the lumbar vertebral body. The dorsal branch of the third lumbar segmental artery penetrated thoracolumbar fascia and formed rich hypodermic capillary net in the region. All patients were followed up from 5 to 8 years with an average of 5.6 years. VAS score of low back pain and leg pain decreased respectively from preoperative 7.6 +/- 1.2, 8.9 +/- 0.9 to 3.6 +/- 0.5, 3.0 +/- 0.4 at final follow-up (P < 0.01); and ODI score decreased from preoperative 44.1 +/- 6.2 to 13.9 +/- 3.6 at final follow-up (P < 0.01). According to ODI score to evaluate the clinical outcomes, 29 cases got excellent results, 11 good, 7 fair, 2 poor.
CONCLUSIONThe pedicle fat grafts with the third lumbar segmental artery and Chitsan can reduce epidural scar formation and prevent peridural fibrosis and adhesion and improve clinical effects of reoperation for lumbar disc herniation.
Adipose Tissue ; pathology ; Adult ; Aged ; Arteries ; pathology ; physiopathology ; Female ; Follow-Up Studies ; Humans ; Intervertebral Disc Displacement ; pathology ; physiopathology ; surgery ; Lumbar Vertebrae ; blood supply ; pathology ; surgery ; Male ; Middle Aged ; Reoperation ; Transplantation ; Treatment Outcome
3.High expression and characterization of human parathyroid hormone in Escherichia coli.
Hong-Qing FANG ; Hong-Mei DAI ; Yan-Ying LI ; Hong-Liang ZHAO ; Bing-Bing DENG ; Chong XUE ; Zhi-Min LIU ; Hou-Chu ZHU ; Qing-Jun MA ; Hui-Peng CHEN
Chinese Journal of Biotechnology 2003;19(1):102-106
Human parathyroid hormone (hPTH) was highly expressed in Escherichia coli by inserted the synthesized whole hPTH cDNA into the vectors pBV220 and pET22b. After expression and disruption, the purified product was acquired through cation exchange chromatography and reverse phase chromatography. From the results of N-terminal sequencing and MALDI-TOF-MS analysis the recombiant prtein was indentified as intact hPTH. In in vitro Bioassays the recombinant hPTH stimulated adenylate cyclase as the standard did. In ovariectomized rats the recombinant hPTH markedly increased the femoral bone mass and bone mineral density.
Amino Acid Sequence
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Animals
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Base Sequence
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Bone Density
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drug effects
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Chromatography, Ion Exchange
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli
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genetics
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metabolism
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Female
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Humans
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Molecular Sequence Data
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Ovariectomy
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Parathyroid Hormone
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chemistry
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genetics
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metabolism
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pharmacology
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Rats
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Rats, Wistar
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Sequence Alignment
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
4.Role of mitochondria pathway in signal transduction of chronic myeloid leukemia.
Zhen-Chuan SHANG ; Ting-Ting SONG ; Yang-Tang HOU ; Li FU ; Tao DENG ; Hai YI ; Tao ZHANG ; Bing-Zhong SUN
Journal of Experimental Hematology 2009;17(2):277-280
This study was aimed to investigate the role of mitochondria pathway in signal transduction of chronic myeloid leukemia (CML). After bcr3/abl2 antisense oligodeoxynucleotide (ASO) was introduced into CML cell line K562 cells by liposomal transfection, the cell viability was detected by MTT assay, the cell apoptosis was determined by flow cytometry (FCM), the mitochondrial membrane potential (DeltaPsi) was labeled by Rhodamine 123 and examined by FCM, and the expression of mitochondrial apoptosis signal transduction pathway related proteins cytochrome C was analyzed by Western blot. The results showed that after K562 cells were exposed to 2 micromol/L of bcr3/abl2 ASO for 24 hours, bcr3/abl2 ASO significantly inhibited cell viability with inhibitory rate of 65.7%, induced the apoptosis of K562 cell line with apoptotic rate of 16.9%, and decreased mitochondrial Deltapsi of K562 cells with the reducing rate of 38.33%, enhanced the expression of cytochrome C with increase of optical density value from 2.33 +/- 0.3 to 4.78 +/- 0.1 by laser photometric scanning. It is concluded that mitochondria pathway plays an important role in signal transduction of chronic myeloid leukemia by directing apoptotic signal transduction.
Apoptosis
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Humans
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K562 Cells
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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metabolism
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Membrane Potential, Mitochondrial
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Membrane Potentials
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Mitochondria
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metabolism
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Oligonucleotides, Antisense
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Signal Transduction
5.Genetic polymorphisms of four STR loci on chromosome X and their forensic applications in a Chinese Han population.
Mei-sen SHI ; Jian-qiang DENG ; Li-bing YUN ; Jing YAN ; Yi-ping HOU
Chinese Journal of Medical Genetics 2005;22(3):268-271
OBJECTIVETo add DXS7133, GATA198A10, DXS9896 and DXS6797 to the panel of forensically validated X chromosome markers, and apply the multiplex amplification system to a population study and forensic analysis on the Hans of Chengdu.
METHODSThe PCR products were detected by the polyacrylamide gel electrophoresis and silver staining method. Hardy-Weinberg equilibrium of females was tested and every forensically interested value was calculated.
RESULTSSequencing revealed that their common sequence motifs were tetranucleotide repeats. Population genetic data were obtained by analyzing 120 unrelated females and 100 males from Chengdu Han ethnic group. In this population, DXS7133, GATA198A10, DXS9896 and DXS6797 exhibited 6, 6, 11, 8 distinguishable alleles respectively. Chi-square test demonstrated that genotype frequencies in females did not depart from Hardy-Weinberg equilibrium. Power of discrimination for female samples for the four loci were 0.7962, 0.8021, 0.9675, and 0.9444. The parentage testing in 32 cases revealed a typical X-linked inheritance and no mutations.
CONCLUSIONDXS7133, GATA198A10, DXS9896 and DXS6797, which are highly polymorphic in Chengdu Han population, are appropriate for individual identification and paternity testing involving a female child.
Asian Continental Ancestry Group ; genetics ; Chi-Square Distribution ; China ; Chromosomes, Human, X ; genetics ; Female ; Gene Frequency ; Genotype ; Humans ; Male ; Microsatellite Repeats ; genetics ; Polymerase Chain Reaction ; Polymorphism, Genetic ; genetics
6.Constructing standard allelic ladders for four short tandem repeat loci and employing them in a population study on Han Nationality of Chengdu in China.
Jian-qiang DENG ; Bing-wu YING ; Mei-sen SHI ; Jing YAN ; Zheng-jun JIA ; Ying-bi LI ; Jin WU ; Ji ZHANG ; Yi-ping HOU
Chinese Journal of Medical Genetics 2005;22(1):49-53
OBJECTIVETo solve the problems in the accuracy and standardization of short tandem repeats-polymerase chain reaction (STR-PCR) typing, the authors adopted the molecular clone technology in producing the standard allelic ladders of D1S1676, D2S2735, D11S1977 and D22S444 loci and applied them in a population study on the Hans in Chengdu, China.
METHODSPCR was used to produce several different allelic fragments of these loci. PCR products were eluted from the gel and re-amplified by PCR. The purified allelic fragments were then blunt-end subcloned individually into the pGEMR-T plasmid vectors and the recombinant were transfected into competent E.coli DH5alpha TM cells. The results of sequencing confirmed that the size and the construction of the inserts were correct. The recombinant plasmids DNA with the inserts were then used as template for re-amplification to generate the four loci standard ladders.
RESULTSThe authors succeeded in producing large quantity of standard allelic ladder of these four loci, with which the genetic polymorphisms of these loci in Chengdu Han population of China were studied.
CONCLUSIONThis method is of high value for forensic DNA typing to construct standard ladders. D1S1676, D2S2735 loci are robust for forensic analysis in Chinese Han population, whereas the value of D11S1977 and D22S444 loci is limited.
Alleles ; Asian Continental Ancestry Group ; genetics ; China ; Genetics, Population ; methods ; Humans ; Microsatellite Repeats ; genetics ; Polymerase Chain Reaction ; Polymorphism, Genetic
7.Preventive and therapeutic effects of recombinant IFN-alpha2b nasal spray on SARS-CoV infection in Macaca mulata.
Hong GAO ; Li-lan ZHANG ; Qiang WEI ; Zhao-jun DUAN ; Xin-ming TU ; Zhi-ai YU ; Wei DENG ; Li-ping ZHANG ; Lin-lin BAO ; Bin ZHANG ; Wei TONG ; Yun-de HOU ; Bing-lin ZHANG ; Lan HUANG ; Chuan QIN
Chinese Journal of Experimental and Clinical Virology 2005;19(3):207-210
OBJECTIVETo study the preventive and therapeutic effects of recombinant IFN-alpha2b for nasal spray on SARS-CoV infection in Macaca mulata (rhesus monkey).
METHODSTen rhesus monkeys were divided into two groups, 5 in interferon group, and 5 in control group. Before and after SARS-CoV attack, the virus was detected in samples such as pharyngeal swab in all the two groups by Real-time PCR (RT-PCR) and virus isolation was performed.
RESULTSAfter virus attack, the level of SARS-CoV-specific IgG and neutralizing antibody were induced by SARS-CoV in the interferon group was weaker than in control group. Hematology items showed no apparent changes after virus attack in treated group. Through pathological examination, the morphology of the lung tissues of two Macaques in the treated group was normal, while the other three displayed the interstitial pneumonia with the thickened septum and infiltration with mononuclear cells. Among which, one monkey showed part of thickened septum fused with each other. These lesions in the interferon treated animals were similar to those seen in the animals in control group, but with smaller scope of pathological changes. No significant abnormity was detected in other organs.
CONCLUSIONRecombinant IFN-alpha2b could effectively interdict or weaken SARS-CoV injury in monkeys.
Animals ; Antiviral Agents ; therapeutic use ; Cercopithecus aethiops ; Disease Models, Animal ; Female ; Interferon-alpha ; therapeutic use ; Lung ; drug effects ; pathology ; virology ; Macaca mulatta ; Male ; Monkey Diseases ; drug therapy ; prevention & control ; virology ; Random Allocation ; Recombinant Proteins ; SARS Virus ; drug effects ; isolation & purification ; Severe Acute Respiratory Syndrome ; drug therapy ; prevention & control ; virology ; Vero Cells
8.YAF2 promotes tumor cell proliferation via targeting cyclin D1 expression
Cong-Cong HOU ; Xiao-Li MA ; Hong CHEN ; Bing-Ren HUANG ; Deng CHEN
Basic & Clinical Medicine 2018;38(6):764-770
Objective To explore the molecular mechanism by which YY1 associated factor 2(YAF2) up-regulates cyclin D1 expression in tumor cells as well as the effect of YAF2-cyclin D1 regulatory loop on tumor cell prolifera-tion. Methods Overexpression and knockdown experiments combined with Western blot and real-time quantitative PCR were used to detect the expression of YAF2 and cyclin D1;Dual luciferase reporter assay was performed to in-vestigate the effect of YAF2 on cyclin D1 promoter activity;Flow cytometry analysis was carried out to elucidate the effect of YAF2 on cell cycle progression through targeting cyclin D1;Colony formation assay was employed to deter-mine cell proliferation under different YAF2 and cyclin D1 expression level. Results YAF2 upregulated the ex-pression of cyclin D1 at both the mRNA(P<0.05) and protein level;YAF2 activated the promoter activity of cyc-lin D1 (P<0.05);YAF2 silencing increased significantly the proportion of cells in G0/G1phase(P<0.0001) and reduced the proportion of cells in S phase by regulating cyclin D1 (P<0.002); YAF2 facilitated the cell colony formation via targeting cyclin D1(P<0.05). Conclusions In tumor cells,YAF2 promotes the expression of cyclin D1,and enhances cell cycle progression and cell proliferation.
9.Preliminary application of CPC/PMMA composite bone cement in kyphoplasty for the elderly.
Xuan-Geng DENG ; Xiao-Ming XIONG ; Wei CUI ; Tao GU ; Dun WAN ; Hua-Gang SHI ; Xing CHEN ; Si-Mao SONG ; Wei HOU ; Guo-Long MEI ; Wen-Bing JIANG
China Journal of Orthopaedics and Traumatology 2020;33(9):831-836
OBJECTIVE:
From the perspective of clinical application to analyze the effectiveness and reliability of CPC/PMMA bone cement in percutaneous kyphoplasty (PKP) for the treatment of elderly patients with osteoporotic thoracolumbar fractures.
METHODS:
A retrospective analysis was performed on 62 patients with osteoporotic compression fracture of single-vertebral thoracic or lumbar segment who underwent PKP surgery and had a bone density less than or equal to -3.0 SD from February 2016 to December 2016. Among them, 23 patients were in CPC/PMMA group, with an average age of (77.6±2.2) years old, 39 patients in PMMA group, with an average age of (77.1±1.1) years old. The indexes between two groups were compared, including the visual analogue scale (VAS), height ratio of anterior vertebra (AVHR), local Cobb angle, cement leakage, new adjacent vertebral fracture(NAVF).
RESULTS:
There were no significant difference in gender, age, follow-up time and preoperative VAS, AVHR, local Cobb angle between two groups (>0.05), at the 1 day after operation, VAS, AVHR, local Cobb angle in all patients got obvious improvement (<0.05), which was no significant difference at 1 day after operation and final follow-up (>0.05). At the same time, there was no statistically significant difference in the incidence of new adjacent vertebral fracture and cement leakage (>0.05). The pain in both groups continued to improve at follow up after operation (<0.05), the local Cobb angle increased (<0.05) and AVHR decreased slightly (<0.05). However, the images of conventional methods (X-ray or CT) could not find signs about CPC degeneration and new bone ingrowth.
CONCLUSION
CPC/PMMA composite bone cement is safe and reliablein PKP for treatment of elderly patients with osteoporotic thoracolumbar fractures, which can effectively relieve pain and maintain vertebral body stability. It has the same curative effect as PMMA bone cement. It was worthy to research more in future, although no direct evidences support the CPC/PMMA composite bone cement can reduce the incidence of adjacent vertebral fracture, CPC degeneration or new bone ingrowth.
Aged
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Bone Cements
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Dinucleoside Phosphates
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Fractures, Compression
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Humans
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Kyphoplasty
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Osteoporotic Fractures
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Polymethyl Methacrylate
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Reproducibility of Results
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Retrospective Studies
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Spinal Fractures
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Treatment Outcome
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Vertebroplasty
10.Application of Dynamic Monitoring of Regional Cerebral Oxygen Saturation for Severe Traumatic Brain Injury
Wei-xin LU ; Bo-ru HOU ; Deng-feng WANG ; Gang WANG ; Ruo-bing BAI ; Gui-zhong YAN ; Dong WANG ; Rui-hao LI ; Xin XUE ; Hai-jun REN
Chinese Journal of Rehabilitation Theory and Practice 2020;26(1):106-109
Objective:To investigate the value of clinical monitoring of regional cerebral oxygen saturation (SctO2) for severe traumatic brain injury (sTBI). Methods:From December, 2017 to January, 2019, 33 patients with sTBI within 24 hours were monitored SctO2, intracranial pressure (ICP) and cerebral perfusion pressure (CPP) with near-infrared spectroscopyonce per six hours for seven days. They were assessed with Glasgow Coma Score (GCS) at admission and Glasgow Outcome Score (GOS) six months after injury. Results:SctO2 was the lowest on the third day of monitoring, and then increased gradually. SctO2 negatevely correlated with ICP (