1.Comparison of different multivessel revascularization methods in type 2 diabetes mellitus patients accom-panying with coronary artery disease
Hong ZHANG ; Kai HOU ; Hongliang CONG ; Ximing LI ; Zuocheng LI
Journal of Endocrine Surgery 2014;(6):452-455
Objective To compare two different revascularization methods in type 2 diabetes mellitus (T2DM) patients with multivessel coronary artery disease treated by percutaneous coronary intervention (PCI) and coronary artery bypass graft ( CABG) .Methods T2DM patients with multivessel disease undergoing success-ful PCI or CABG were enrolled in the study .They were diagnosed by coronary angiography ( CAG) in Tianjin Chest Hospital from May 2009 to May 2010 whose.The patients were followed up for 3 years.The information of patients including physical performance , clinical features , and laboratory examination results were collected .The major ad-verse cardio cerebral events(MACCE)including death, myocardial infarction(MI), revascularization, angina pecto-ris, heart failure, and stroke were collected.Results During the 3 years of follow-up, MACCE(31.58% vs 17.68%, P<0.01), death(4.82%vs 1.10%, P<0.05), MI(4.39%vs 1.10%, P<0.05), angina pectoris (17.27%vs 10.50%, P<0.05)occurred more frequently in PCI group than in CABG group .Conclusion Evi-dences now tend to support CABG for revascularization in T 2DM patients with multivessel disease .
2.Research progress of traditional Chinese medicine formulas and active compounds in the treatment of non-alcoholic fatty liver disease by regulating gut microbiota
Yan LI ; Wei-ze ZHU ; Hou-kai LI
Acta Pharmaceutica Sinica 2022;57(12):3451-3464
Non-alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases. However, due to its complex pathogenesis, there are no officially approved drugs for NAFLD treatment currently. Therefore, it is extremely urgent to find safe and effective anti-NAFLD drugs. Nowadays, lipid-lowering drugs are the main option for NAFLD therapy, but the clinical efficacy of chemical drugs is also very limited, as well as the frequent side effects or adverse reactions. Traditional Chinese medicine (TCM) has attracted more and more attention in the treatment of NAFLD due to its unique advantages through multiple targets and pathways with few side effects. In recent years, numerous studies have demonstrated that the imbalance of gut microbiota plays an important role in the occurrence and development of NAFLD. This review systematically summarizes the experimental and clinical evidences of TCM active compounds and TCM prescription involved in the regulation of intestinal flora in the treatment of NAFLD in recent years, so as to provide a reference for further exploring the pathogenesis of NAFLD and exploring TCM treatment methods.
3.Study of relationship between depressive symptoms in patients with type 2 diabetes and adiponectin levels
Xiaojing LI ; Ningning HOU ; Jing WANG ; Kai LIU
Chinese Journal of Postgraduates of Medicine 2012;(z2):19-21
Objective To explore the correlation of adiponectin (ADP) and type 2 diabetes mellitus (T2DM) depressive symptoms.Methods Random sample of hospitalized confirmed patients with T2DM were 76 cases,who were divided in to T2DM group (DM group,30 cases) and T2DM with depressive symptoms group (DD group,40 cases) and randomly selected 36 cases in the medical center as control group.The level of height,weight,waist circumference,hip circumference,plasma ADP,fasting plasma glucose (FPG),total cholesterol (TC),triglyceride (TG),high density lipoprotein cholesterol (HDL-C),low-density lipoprotein protein cholesterol (LDL-C),C-reactive protein (CRP),fasting plasma cortisol (COR),fasting insulin (FINS) were measured.Body mass index (BMI),waist to hip ratio (WHR) and insulin resistance index (HOMA-IR) was calculated.Results The plasma ADP level in DD group was lower than that in DM group and control group [(3.05 ± 1.07) g/L vs.(4.06 ± 1.12),(6.97 ± 1.64) g/L],there was significant difference (P < 0.01).The plasma ADP level was negatively correlated with CRP,HOMA-IR,TC,FPG and SDS,but positively correlated with HDL-C.HDL-C and SDS was the independent risk factor influencing the ADP level in DD group.Conclusions T2DM patients with depressive symptoms exist the greater insulin resistance,the level of plasma ADP is specific drop; the lower level of plasma ADP is an independent risk factor for T2DM patients with depressive symptoms,there is independent correlation between them.
4.The changes of brain-gut peptides and immunologic function in the rats before and after relief of biliary obstruction
Xuting ZHI ; Xiangqin HOU ; Tao LI ; Kai SUN ; Fengjun LIU
Chinese Journal of Current Advances in General Surgery 1999;0(04):-
Objective:To investigate the changes and the adjusting effects of brain-gut peptides (BGP) on immunologic function in the rats before and after relief of biliary obstruction.Methods:One hundred Wistar rats were randomly divided into five groups,including sham common bile duct ligation group (group A),common bile duct ligation group (group B),sham relief of biliary obstruction + normal saline group (group C) ,relief of biliary obstruction + normal saline group (group D) and relief of biliary obstruction + Sandostatin (group E).At 1d,7d after the operation of each group,the changes of plasma vasoactive intestional peptide (VIP),substance P(SP),serum interleukin-2(IL-2) and serum T-lymphocytic subsets (CD4 +,CD8 +)were determinded by radioimmunoassay and flowcytometry .Results:Plasma VIP and SP were increased in group B compared with group A(P
5.Sudden cardiac death of incarcerated prisoners: a study of 75 cases.
Lan YU ; Li-Min DONG ; Xian-Jun HOU ; Kai SHI ; Kai XU
Journal of Forensic Medicine 2014;30(2):112-116
OBJECTIVE:
To investigate the characteristics and influencing factors leading to sudden cardiac death (SCD) of incarcerated prisoners.
METHODS:
Seventy-five SCD cases of prisoners between 2000 and 2013 in Henan province were collected, and environment, psychological and physical factors were retrospectively analyzed. Combined with histopathological results, specific factors of SCD were also studied.
RESULTS:
In the 75 cases, 21 cases (28%) had definite chronic past medical histories, and 75 cases (100%) had cardiovascular disease confirmed by autopsy.
CONCLUSION
Due to presence of the potential cardiac diseases, special incarcerated environment, psychological stress, and body-restraint might be the precipitating factors in SCD of those prisoners.
Autopsy
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Cardiovascular Diseases
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Death, Sudden, Cardiac
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Environment
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Humans
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Mental Disorders
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Prisoners
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Retrospective Studies
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Stress, Psychological
6.Expressions of glucose transporter 1 and 3 proteins are changing with blood glucose concentration in diabetic rat brain
Yu-Xin XIAN ; Wei-Kai HOU ; Xin-Guo HOU ; Ming DONG ; Li CHEN ;
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Streptozotocin-induced diabetic model was prepared in Wistar rats and the blood glucose levels were controlled at 3 levels of<10,10-14 mmol/L or>16.7 mmol/L by insulin,and changes of glucose transporter(GLUT)1 and 3 protein expressions of brain were observed in control rats and diabetic rats with different blood glucose levels by immunohistochemistry.The results showed that chronic hyperglycemia could decrease the protein expressions of GLUT 1 and GLUT3.
7.Mechanism study of Si Miao Formula on alleviating insulin resistance by increasing the abundance of Akkermansia muciniphila in mice
Juan YAN ; Li-li SHENG ; Yan LI ; Yi-yang BAO ; Hou-kai LI
Acta Pharmaceutica Sinica 2022;57(12):3502-3512
In our previous study, we found that Si Miao Formula (SMF) had the effect of improving the disorder of glucose metabolism caused by high fat and high sucrose diet, and significantly altered the composition of gut microbiota, especially increasing the level of
8.Extraction and Its Application of the Effective Components from Shrimp in Detection for sIgE
Jie REN ; Lixin SHAN ; Shaoshen LI ; Kai CHEN ; Liying HOU ; Huiqiang LI
Tianjin Medical Journal 2014;(5):462-465
Objective To improve the detecting sensitivity of serum specific IgE (sIgE) by improving the quality of coated antigen in shrimp. Methods The extracts from shrimp protein was prepared. Western blot assay was used to identify the major allergenic protein components. The protein components>55 ku were separated by Sephadex gel chromatography. SDS-PAGE technology was used to analyze proteins. Samples of shrimp protein and proteins>55 ku were used as the coat-ing antigen to coat 96 microplate respectively. Western blot assay and ELISA were used to evaluate preliminary sensitivity of the purified antigen for detecting sIgE. Results Immunoblot experiments showed that the protein>55 ku was the main aller-genic protein component of shrimp. Those >55 ku proteins were separated successfully by Sephadex gel chromatography, showing 10 identifiable bands in SDS-PAGE. Dot-pot immunoassay showed that proteins>55 ku used as coated antigens could improve the spots density of the weak serum. Meanwhile, the result of ELISA showed that sIgE detection value in-creased 92.9%in patients with shrimp allergy after coating effective antigens. Conclusion The detecting sensitivity of sIgE can be improved by using effective protein components of shrimp as coated antigens.
9.Determination of urinary polypeptide patterns of glomerulonephritis by magnetic bead separation and MALDI-TOF MS
Jie WU ; Fan LI ; Yuausheng XIE ; Jie WANG ; Na WANG ; Zhang YIN ; Kai HOU ; Xiangmei CHEN
Chinese Journal of Nephrology 2009;25(8):596-600
Objective To determine the urinary polypeptide patterns of glomerulonephritis by magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology. Methods Urinary samples of 29 healthy volunteers and 34 patients with glomerulonephritis, including 10 cases of IgA nephropathy (IgAN), 10 cases of membranous nephropathy (MN), 9 cases of minimal change disease (MCD) and 5 cases of lupus nephritis (LN), were collected and separated by magnetic bead,and were screened for polypeptide patterns with a novel high throughput method, MALDI-TOF MS. Results Under the relative molecular weights 10 000 Da, 85 protein peaks were detected in healthy controls group and 109 protein peaks were detected in glomerulonephritis group. Six peaks of 3371.5 Da, 4026.35 Da, 4085.32 Da, 4116.96 Da, 4126.32 Da and 9527.31 Da were up-regulated,while 8 peaks of 861.28 Da, 1205.41 Da, 1642.52 Da, 1913.15 Da, 1976.52 Da, 2087.74 Da, 2193.47 Da and 3015.57 Da were down-regulated by more than 2 folds (P<0.01) in glomerulonephritis group as compared to healthy controls. Urinary polypeptide patterns in different diseases differed significantly from each other, indicating specific disease pattern of polypeptide excretion. Conclusions MALDI-TOF MS is a fast, convenient and high throughput analyzing method capable of screening some relative specific, potential biomarkers from the urine of glomerulonephritis patients thus it possesses better clinical value.
10.Stress dynamic CT myocardial perfusion imaging combined with coronary CT angiography for the detection of ischemic coronary artery disease
Yang GAO ; Chengying WANG ; Yanli ZHOU ; Zhihui HOU ; Weihua YIN ; Zhennan LI ; Kai SUN ; Bin LYU
Chinese Journal of Radiology 2017;51(4):246-250
Objective To assess effects of stress dynamic CT myocardial perfusion imaging (CT-MPI) combined with coronary CT angiography (CCTA) on the diagnosis of myocardial perfusion defects in coronary artery disease (CAD). Methods Patients with CAD diagnosed by CCTA underwent ATP stress CT-MPI examination. Single-photon emission computed tomography (SPECT) myocardial perfusion imaging (SPECT-MPI) was performed within one week and set as the reference standard. CT-MPI results were qualitatively analyzed, and myocardial blood flow (MBF), myocardial blood volume (MBV) as well as time to peak (TTP) were quantified according to CT-MPI. Effects of CCTA, CT-MPI, and CT-MPI combined with CCTA on predicting myocardial perfusion defects were assessed in comparison with NMPI. Results Thirty patients [(54.8±8.4)years] were enrolled in our study, 20 were men (68%). MBF [(79.3±18.0) versus (135.1± 35.2) ml·100 ml-1·min-1] and MBV [(8.9±2.9) versus (13.8±8.9) ml/100 ml] were significantly decreased in hypoperfused segments compared with normal segments, while TTP was increased in hypoperfused segments [(13.9 ± 2.5)s] compared with normal segments [(9.1 ± 2.1)s] (t=0.302, 0.866 and 0.024 respectively, all P values<0.01). The sensitivity, specificity of CT-MPI for identifying segments with perfusion defects were 91.3%(147/161), 84.6%(281/332), respectively. On a per-vessel basis, the area under the receiver operating characteristic curve for predicting myocardial perfusion defects were 0.635(95%CI:0.517—0.753) for CCTA, 0.709(95%CI:0.599—0.819)for CT-MPI, and 0.837(95%CI:0.749—0.925)for CT-MPI combined with CCTA, respectively. Conclusions The performance of stress dynamic CT-MPI in the diagnosis of myocardial perfusion defects in CAD was good. One-stop examination of CT-MPI combined with CCTA improves the diagnostic accuracy for identifying flow-obstructing stenosis.