Proteomics is an emerging discipline which studies the composition of proteins and their transformation rules at the cellular level and the overall level.Recently,proteomics has been used in a variety of studies of tumors.And it has made some progresses in explaining the mechanisms of tumors' genesis and development,discovering new specific markers for tumors and new targets for drug treatment,and forecasting tumors' biological behaviors.

Objective To explore nurses' dispositional optimism and mental health and disscuss the impact of dispositional optimism on nurses' mental health in order to provide evidences for promoting the level of nurses' mental health. Methods Adopting Revised Life Orientation Test (LOT-R) and Symptom Checklist 90 (SCL-90) ,the researchers carried on the questionaire investigation to a total of 240 nurses. Results Unmarried nurses were more optimistic than married ones(15.53 ± 3.48,14.39 ± 2.88) ,and the difference had statistic significance(P ＜ 0.01). The positive detection rate of nurses' psychological symptoms was 6.7% ～ 26.5%. Compulsion, interpersonal sensitivity and depression were nurses' main psychological symptoms. Compared to the national female norm, the somatization, depression, anxiety, psychosis factor scores, total score and the number of positive items of SCL-90(1.49±0.45,1.70±0.42, 1.55 ±0.43,1.46±0.38,1.40±0.37,1.50±0.35,37.23±22.02)were higher, and the difference had statistic significance(P＜ 0.05). Dispositional optimism postively correlated with the level of nurses' mental health(P＜ 0. 001). Conclusions The general condition of nurses' mental health is not good enough, and dispositional optimism has good forecast function to nurse's mental healthy. Therefore, maintaining positive and optimistic attitude will be helpful to nurses' mental health.

Acute kidney injury (AKI) represents a common and devastating problem in clinical medicine. The lack of early biomarkers for AKI has led to a delay in initiating potentially effective therapies.Identification of novel biomarkers for AKI has progressed to the clinical phase of the biomarker discovery process. The most promising AKI biomarkers include neutrophil gelatinase-associated lipocalin, IL-18, kidney injury molecule-1 and liver-type fatty acid binding protein. In this article, we review the study of neutrophil gelatinase-associated lipocalin,IL-18, kidney injury molecule-1 and liver-type fatty acid binding protein in recent years,in order to provide the theory basis for the clinical application of the early biomarkers for AKI.

Objective To establish a method for quality control of Tangui Weining granules. Methods TLC was used for the identification of Lignum Santali Albi and Cortex Cinnamomi. HPLC was used for the determination of Glycyrrhizin. The chromatographic conditions were as follows: Diamonsil C18(4. 6 mm ×250 mm, 5 μm)was used as a stationary phase, the mobile phase consisted of methanol-O. 2 mol/L ammonium acetate solution-acetic acid (60 : 40 : 1), the ducible, and the negative controll did not show interference. Glycyrrhizin was separated completely by HPLC and showed a good linearity in the range of 0. 1568 to 1. 568μg, r=0. 999 8, the average recovery was 99.69 %, and RSD was 0. 58 %. Conclusion TLC is simple, convenient and accurate. The method of HPLC is proved to be simple and accu-rate with a good reproducibility and can be used for the inspection of Glycyrrhizin in Tangui Weining Granules. This study provides a foundation for quality control of Tangui Weining granules.

Cytokeratins can maintain the morphous and function of normal cells. Clinically, cytokera-tins are used to diagnose several tumors as tumor markers. Recently, numerous studies indicate that the abnor-mal expression, mutation in the site of phosphorylation and hyperphosphorylation have relationship with the mechnism of tumorigenesis. Reseach of the relationship is important for the diagnosis, therapy and prognosis of tumor.

Objective To explore diagnostic basis for early renal lnjuryo Methods Enzyme-linked immunosorbent assay wag used,urine protein icroalbumin,Micoalbuminl,retinol binding protein.Urinary N-acetyle B-D-glucosaminidase was determineby chromatometry.Urinary creatinine was determined with enzymic method.Results The levels of urinary transferrin,miroalbumin and NAG in the controls were Alb/Cr(1.93±0.42(mg/mmol),UPI/Cr 47.61±5.86(mg/mmol),RBP/cr:24.41±3.55(mg/mmol),NAG/Cr.0.96±0.58(u/mmol).The levels of urirary transferrin,microalbumin and NAG in urinary albumin negative groups(diabetes,hypertension,lupus erythematosus,Wethra infection)were higher than those in the controls(P＜0.01),positive rates of determining one or two of the four items were lowerthan that of determining Alb UPl RBP and NAG.together.Determing for items together would raise the positive rate to 92% in diabetes,80%in hypertension,85%in lupus erytematosus,76%in urethra infection respectively.Conclusion It is sensitive and reliable tO detect Alb,UPI,RBP and NAG together for diagnosis of early renal injury.

A disintegrin and metalloproteinase 10 is a zinc dependent transmembrane type Ⅰ multi-domain of protein which is highly conserved in evolution and mediates protein maturation and ectodomain shedding, with protein hydrolysis, cellular adhesion, cellular fusion and signal transmission.This article reviews its structure and function, the substrate and relative signaling pathways, the factors influencing its expression and catalytic activity,the research progress in urinary tract diseases.

Objective To evaluate the efficacy and safety for duloxetine alone and combined with repetitive transcranial magnetic stimulation in the treatment of generalized anxiety disorder (GAD).Methods 60 GAD patients were randomly divided into two groups using number table.30 cases in the control group were given duloxetine treat-ment,and 30 patients in the observation group were given duloxetine combined with repetitive transcranial magnetic stimulation treatment.The clinical efficacy and adverse reactions were compared.Results The total effective rate of the control group was 70.0 %,which of the observation group was 76.7%,the difference between the two groups was not significant (χ2 =2.44,P >0.05).When treatment 1,2,4,6 weeks,Hamilton Anxiety Scale (HAMA)score were significantly lower than before treatment(t =6.23,7.44,7.73,7.78,8.42,8.53,9.60,9.81,all P <0.05).1 week after treatment,HAMA score of the observation group was significantly lower than the control group (t =6.77,P <0.05).2,4,6 weeks after treatment,HAMA score between the two groups had no significant differences (t =2.20, 1.87,2.41,all P >0.05).The incidence rate of adverse reactions of the observation group (23.3%)was significantly lower than the control group (36.7%),the difference was statistically significant (χ2 =7.44,P <0.05 ). Conclusion The efficacy of duloxetine alone and combined with repetitive transcranial magnetic stimulation in treat-ment of GAD is fairly,but combination therapy has faster onset,better security,it is possible to shorten the time of onset of drug and improve patients'treatment compliance,which is more suitable for clinical application.

Objective To explore the correlation of high-sensitivity C-reactive protein(hs-CRP) with the severity of vascular dementia(VaD) patients Methods All participants underwent MMSE and ADAS-cog and had their blood samples taken for hs-CRP measurement before the initiation of treatment.Patients in the Alzheimer's disease (AD)group and the VaD group received treatments according to the recommended guidelines for dementia.Patients were monitored for six months,during which each course of treatment lasted one month.Participants received assessment on the severity of dementia and underwent blood tests for hs-CRP at the conclusion of each course of treatment.Results Patients with VaD had higher hs-CRP levels than those in the control group.As the severity of dementia improved,hs-CRP levels declined accordingly.There was a statistically significant difference in hs-CRP levels between patients with moderate to severe VaD and patients with mild VaD(P＜ 0.05).Conclusions The ha-CRP level shows a higher correlation with VaD than with AD.Adjustment of treatment plans may be based on blood hs-CRP levels measured at the end of each course of treatment.

Objective To prepare everolimus self-microemulsifying drug delivery systems (Everolimus-SMEDDS) and evaluate its physicochemical properties.Methods Everolimus-SMEDDS were developed by using rational blends of components with good solubilizing ability for everolimus which were selected based on solubility investigation.Then, pseudo-ternary phase diagram was constructed to determine the self-emulsifying region.The appearance, morphology, particle size distribution, Zeta potential and in vitro drug release behavior of Everolimus-SMEDDS were investigated after being diluted by water.Results An optimal Everolimus-SMEDDS consisting of ethylis oleas, Solutol HS 15 and propylene glycol were identified, and the optimum proportion was 8∶1∶1.Upon mixing with water, Everolimus-SMEDDS formed a clear and transparent microemulsion solution with homogeneous small spherical as seen in transmission electron microscopy.Particle size and Zeta potential of Everolimus-SMEDDS were found to be (48.7±25.1) nm and (-14.2±2.5) mV, respectively.The in vitro release profile indicated that the accumulated release of everolimus in SMEDDS reached up to nearly 100% within 30 min.Conclusion The in vitro dissolution of everolimus was remarkably improved by SMEDDS.It can provide reference for the new dosage form of everolimus.