Objective To prepare everolimus self-microemulsifying drug delivery systems (Everolimus-SMEDDS) and evaluate its physicochemical properties.Methods Everolimus-SMEDDS were developed by using rational blends of components with good solubilizing ability for everolimus which were selected based on solubility investigation.Then, pseudo-ternary phase diagram was constructed to determine the self-emulsifying region.The appearance, morphology, particle size distribution, Zeta potential and in vitro drug release behavior of Everolimus-SMEDDS were investigated after being diluted by water.Results An optimal Everolimus-SMEDDS consisting of ethylis oleas, Solutol HS 15 and propylene glycol were identified, and the optimum proportion was 8∶1∶1.Upon mixing with water, Everolimus-SMEDDS formed a clear and transparent microemulsion solution with homogeneous small spherical as seen in transmission electron microscopy.Particle size and Zeta potential of Everolimus-SMEDDS were found to be (48.7±25.1) nm and (-14.2±2.5) mV, respectively.The in vitro release profile indicated that the accumulated release of everolimus in SMEDDS reached up to nearly 100% within 30 min.Conclusion The in vitro dissolution of everolimus was remarkably improved by SMEDDS.It can provide reference for the new dosage form of everolimus.

Objective To observe the clinical effects of Shenqi Fuzheng Injection in treatment of sepsis in children and its effect on the serum levels of high mobility group protein-1 and tumor necrosis factor-α, lactic acid, C reactive protein, procalcitonin levels.Methods 140 patients with sepsis were chosen, and randomly divided into two groups, the observation group and the control group.Two groups were given treatment by the national standard of sepsis, while the control group only received basic treatment, the observation group on the basis of conventional treatment combined with Shenqi Fuzheng injection.Two groups of clinical curative effect and the levels of serum HMG-1, TNF-α, LA, CRP, PCT level were observed. Results After treatment, the observation group total effective rate (95.71%) was significantly higher than control group (70.00%),χ2 =16.29, P<0.01.Two groups before treatment, TNF-αand HMG-1 had no statistically significant difference.After treatment, 2 groups of HMG -1, TNF-αsignificantly lower than before treatment (P<0.01),which HMG-1,and TNF-αreduced significantly in observation group (P<0.01).Serum HMG-1 were positively correlated with TNF-αbefore and after treatment (P<0.05).After treatment, LA, CRP and PCT in 2 groups were significantly different compared with before treatment (P<0.01), and the observation group decreased significantly than control group (P<0.01).Conclusion The effect of Shenqi Fuzheng Injection in the treatment of children with sepsis is significant, which is worthy of clinical application.

With deeper understanding for intervertebral disc degeneration,the development and clinical application of protocols in molecular biology,biological intervention in initial stage of intervertebral disc degeneration can retard even reverse the degeneration process. Gene therapy of intervertebral disc degeneration refers to the selection of animal models and gene carriers,application of target genes,etc. It is the key for gene therapy to build human-like animal model,select proper gene carrier and apply ideal target genes. Prior to clinical application,the safety and efficacy of gene therapy should be proved,thus many problems deserve further study.

BACKGROUND: Studies on substitute cells of bladder smooth muscle cells are in the early period at present. Mesenchymal stem cell transplantation is an ideal method. Human amniotic mesenchymal cells can differentiate into cardiomyocytes and nerve growth factor and promote local structure repair. OBJECTIVE: To investigate the differentiation of human amniotic mesenchymal cells into smooth muscle cells following transplantation, and the effects on bladder muscle layer. DESIGN, TIME AND SETTING: The cytology in vitro study was performed at the Japan Xinzhou University from October 2006 to April 2007. MATERIALS: Human amnion was obtained from healthy full-term puerperal. A total of 18 clean female Sprague Dawley rats were randomly and equally assigned into normal bladder cell transplantation group, frostbite bladder control group, and frostbite bladder cell transplantation group. METHODS: Amnion was cut and mixed in DMEM containing trypsin. Epithelial cells were removed prior to tissues were incubated in DMEM, supplemented with collagenase and DNA enzyme. Human amniotic mesenchymal cells were harvested for use. Posterior vertex of urinary bladder was frozen using a -70 ℃ iron rod in rats of the frostbite bladder control group and frostbite bladder cell transplantation group. Hematoma appeared at the frostbite region of the bladder three days later. 100 ?L DMEM was injected into the hematoma of the rats of the frostbite bladder control group, while an equal volume of human amniotic mesenchymal cell suspension (105 cells) was injected into the hematoma of the rats of the frostbite bladder cell transplantation group. Human amniotic mesenchymal cell suspension was implanted into the normal rat bladder in the normal bladder cell transplantation group. After three weeks, the bladder tissue including partial urethra was used for bladder sample preparation. MAIN OUTCOME MEASURES: Bladder smooth muscle repair was observed using hematoxylin-eosin staining. The differentiation of human amniotic mesenchymal cells in the wall of urinary bladder was detected using fluorescence immunohistochemistry. RESULTS: Compared with the normal bladder cell transplantation group, normal wall structure of urinary bladder, few fibrosis and good proliferation of smooth muscle cells were detected in the frostbite bladder cell transplantation group, while the wall of urinary bladder was slowly repaired, and disorder muscle structure, fibrosis, scar-like shape, and inflammatory cells were found in the frostbite bladder control group. Three weeks later, human amniotic mesenchymal cells were not seen in the normal bladder tissue. A large number of human amniotic mesenchymal cells was lived and some of them had differentiated into the smooth muscle cells in the frostbite bladder cell transplantation group. CONCLUSION: Human amniotic mesenchymal cells had the potential to differentiate into bladder smooth muscle cells and promoted self-repair of the wall of urinary bladder.

Objective To evaluate the effectiveness of UV-B radiat io n exclusively on a variety type of leukoderma .Methods In open trail, 45 cases of a variety of leukoderma were randomly selected including preg nant striates (20 cases), stretch marks (1 case), postoperative petechia (4 case s), traumatic petechia (10 cases), radiation petechia (2 cases), generalized vit iligo (6 cases) and leukoderma with unknown cause (2 cases) were treated with Re lume UV-B re-pigmentation generator (photo range 290-320 mm), once per week with an adjusting dose for 3-20 treatments. Results 42 cases c ompleted with followed-up after 14 months of UV-B radiation microphototherapy. 64.3% (27 cases) achieved excellent results, including 10 cases of pregnant str iates, 8 cases of traumatic petechia, 4 cases of postoperative petechia and 5 c ases of peneralized vitiligo. 28.6% patients were satisfied with results (12 ca ses), including 7 cases of pregnant striates, 1 case of traumatic petechia, 1 c ase of generalized vitiligo, 1 case of stretch mark and 2 cases of leukoderma wi th unknown causes. 7.1% (3 cases) had no effect. 33.3% (14 cases) required suppl emental treatment after 2-5 months of the initial therapy. Conclusion The UV-B radiation microphototherapy is effective in restoring pigmenta tion in those affected by a variety of leukoderma except radiation petechia. The result of repigmentation is related to the type of leukoderma.

Zebrafish have been emerging as a valuable model for kidney research , including podocyte biology , nephrogenesis and kidney disease .The zebrafish kidney has similar structure , common function and the same cell types with human kidney .Rapid development and feasible genetic editing approaches in zebrafish provide an ideal model for high -throughput gene screening involved in kidney disease and developmental process .Loss or injury of podocytes is the key mechanism , which can lead to proteinuria , glomerulo-sclerosis and renal failure .Here, we introduce structure and function of zebrafish kidney , filtration estimating assays , podocytes injury model and the highlights of recent zebrafish podocytes studies .

Colorectal cancer is a common malignant tumor in digestive system ,with an increasing inci-dence rate and case fatality rate all over the world in recent decades .At present,chemotherapy still plays a very important role in the treatment of advanced colorectal cancer .Targeted therapy brings new hope to patients ,and new chemotherapy drugs and targeted drugs have been widely used in clinics .Patient′s life quality has been great-ly improved,survival time has been significantly prolonged ,the combination of chemotherapy and targeted therapy has also become a hot research area in the treatment of metastatic colorectal cancer .XELOX ( capecitabine plus oxaliplatin)as a first-line chemotherapy regimen in treating metastatic colorectal cancer (mCRC)can obtain good therapeutic effect ,the side effects of XELOX are light and well tolerated;Bevacizumab ,as a new type of targeted anti-tumor drugs ,shows a good effect in inhibiting tumor growth and reducing its hematogenous spread risk .Bev-acizumab in combination with XELOX regimen for treating mCRC has been widely investigated and reported .This review gives a brief summary on the efficacy and safety of the combined administration of Bevacizumab and XE -LOX.

Since 1980s, medical clinical practice turns towards evidence-based medicine model from experimental medical model, traditional TCM diagnosis of tumor difficultly link up with diagnostic methods of modern medicine, and syndrome differentiation typing is difficultly qualified and unified. Clinical studies often lack strict and reasonable design and rigorous manipulation standardization, and do not rapidly adapt changes of modern medicine model. Therefore. in the present paper it is put forward that standardization of clinical diagnosis and assessment of therapeutic effects should be further completed. First establishing and adopting randomized control test of small scale and single center, and using systematical assessing techniques to scientifically evaluate results of relative studies; and scientifically and systematically systematize and strictly re-test and verify rich clinical experiences of famous and older physicians of TCM. In brief, principles of evidence-based medicine should be adopt to guide and standard TCM diagnosis and treatment and scientific studies of malignant tumor.

Diabetes mellitus is particularly prevalent among aging people in recent years.Compared with diabetes in young and middle aged,geriatric diabetes has specific pathophysiology.Macrovascular complications of Type 2 diabetes including coronary heart disease,myocardial infarction,heart failure,stroke and peripheral artery disease,cause increased disability and mortality in aged populations.In order to prevent macrovascular complications,management strategies should be taken in treating hyperglycemia,hypertension,dyslipidemia,and hypercoagulability.

BACKGROUND:Clinical practice verified that percutaneous vertebroplasty in the treatment of osteoporotic vertebral compression fractures obtained obvious curative effects.
OBJECTIVE:To compare the therapeutic effect of percutaneous vertebroplasty for treatment for osteoporotic vertebral compression fractures with or without intravertebral cleft, in order to evaluate the efficacy and safety of percutaneous vertebroplasty for treatment of osteoporotic vertebral compression fractures.
METHODS:A total of 76 patients with osteoporotic vertebral compression fractures were selected and divided into two groups. Experimental group consisted of 27 patients with intravertebral cleft. Control group comprised 49 patients without intravertebral cleft. After vertebroplasty, visual analogue scale and Oswestry disability index, changes of anterior vertebral height, and types and characteristics of bone cement leakage were compared between the two groups.
RESULTS AND CONCLUSION:Visual analogue scale and Oswestry disability index were significantly decreased after treatment in the two groups (P<0.05). No significant differences in visual analogue scale, Oswestry disability index and incidence of bone cement leakage were detected between the two groups after treatment (P>0.05). No significant difference in the height of injured vertebra was detectable between pretreatment and posttreatment in the two groups (P>0.05). These results suggested that vertebroplasty is an effective method for lessening the pain in osteoporotic vertebral compression fractures in patients with or without intravertebral cleft, improving spinal activity function and elevating social activities. However, percutaneous vertebroplasty cannot effectively recover the height of the vertebra.