OBJECTIVE: To prepare Berberine hydrochloride solid lipid nanoparticles(BH-SLN). METHODS: BH-SL-N was prepared by conventional rotary-evaporated film-ultrasonication method. Based on the single-factor experiment, BH-SLN was prepared using orthogonal design for optimization of formulation and technology. The quality of the obtained BH-SLN was evaluated. RESULTS:BH-SLN had even and regular appearance with a mean diameter of 60.5 nm,Zeta potential of —29.7 mV,drug loading of 8.69%, and entrapment ratio of 97.58%. CONCLUSION: The entrapment ratio of BH-SLN is high and the stability is good for BH-SLN prepared by conventional rotary-evaporated film-ultrasonication method, and the method is reliable.

Objective To prepare the glycyrrhizic acid solid lipid nanoparticles(GL-SLN).Methods GL-SLN was prepared by conventional rotary-evaporated film-ultrasonication method.Based on the single-factor experiment,GL-SLN was prepared using orthogonal design for optimization of formulation and technology.The obtained GL-SLN was also evaluated.Results GL-SLN had even and regular appearance and the average diameter was 75.8 nm.Zeta potential was-19.7 mV.The drug loading was 8.27% and the entrapment ratio was 91.76%.Conclusion The entrapment ratio of GL-SLN and the stability of GL-SLN prepared by conventional rotary-evaporated film-ultrasonication method are high and good.

The abilities of self-study,thinking and communication are much more emphasized in the problem based learning(PBL)than in the conventional curriculum.From the view of the inherent relationship between the ability training and PBL,this paper probes into the characteristics of PBL to find the way of fitting the situation of our country.It is essential to the successful teaching and training more high quality students.

Humans ; Multiple Myeloma ; therapy

Combined Modality Therapy ; Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Genetic Therapy ; Hepatitis B ; complications ; drug therapy ; Humans ; Liver Cirrhosis ; drug therapy ; etiology ; Medicine, Chinese Traditional ; Phytotherapy

ObjectiveTo investigate the association between serum level of osteopotin(OPN) and disease activity of rheumatoid arthritis (RA) patients, and explore the importance of OPN in the pathogenesis of interstitial lung disease (ILD) in RA. MethodsSixty-five RA patients and 20 healthy controls were pros-pectively enrolled. RA patients were divided into active group(n=43) and inactive group(n=22), and ILD groups (n=24) and non-ILD group (n=41). Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of OPN in patients with RA and healthy controls, and the relationship between OPN and other clinical and laboratory findings were analyzed.Results① Serum OPN tended to be significantly higher in RA patients (median, 18.0 ng/ml) than in the healthy controls (median, 14.3 ng/ml), P<0.01; ②The serum level of OPN in RA patients showed a significant positive correlation with the course of disease, numbers of tender joints , ESR and CRP, but no positive relationship was found in number of swollen joints; ③ The serum level of OPN was significantly higher in RA-ILD patients(median, 20.0 ng/ml) than that in non-lLD (median, 17.0 ng/ml, P<0.05). And there was remarkable negative correlation between the concentration of serum OPN and the value of PaO2, but no association was found with pulmonary function %VC and %DLCO. ④ Compared with the non-ILD group, the ILD group had more active disease in terms of tender joint counts and swollen joint counts, ESR, CRP(P<0.01) and the serum titer of RF-IgM,(P<0.05). ConclusionOPN plays a role in the pathogenesis of RA and is related to the disease activity. It may serve as an active disease inflammatory marker of RA . OPN may be involved in the pathogenesis of RA related ILD and is associated with the severity of pulmonary damage.

Antineoplastic Agents ; administration & dosage ; therapeutic use ; Hematologic Neoplasms ; drug therapy ; Humans

Humans ; Immunoglobulin D ; Male ; Middle Aged ; Multiple Myeloma ; classification ; complications ; Testicular Diseases ; etiology

Aged ; Amyloidosis ; complications ; Factor X Deficiency ; complications ; Humans ; Immunoglobulin Light-chain Amyloidosis ; Male

Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Multiple Myeloma ; genetics ; metabolism ; pathology ; Polycomb Repressive Complex 1 ; genetics ; metabolism ; Prognosis ; RNA, Messenger ; genetics