The usefulness of E-cadherin immunostaining as a marker of malignancy in the body fluids was investigated in the present study. Thirty-three histologically proven cases of cell blocks from the pleural, peritoneal, and pericardial fluids were studied by immunocytochemistry for E-cadherin antibody using LSAB method. These cases were cytologically diagnosed as adenocarcinoma (25 cases) and atypical cells (8 cases). Tumor cells showed strong positive membranous staining for E-cadherin antibody in 21 out of 25 cases (84%) of adenocarcinoma. E-cadherin staining was not found in 6 of 8 cases of suspicious maligancy. The sensitivity and specificity were 84% and 75%, respectively. Reactive mesothelial cells and inflammatory cells scattered were all negative. In conclusion, E-cadherin is an useful adjunctive marker to distinguish reactive mesothelial cells from the carcinoma cells in the body fluids.
Sensitivity and Specificity ; Adenocarcinoma ; Neoplasm Metastasis

No abstract available.
Needles* ; Prostatitis*

PURPOSE: Norepinephrine has modulatory effects on neuronal excitability and, in some cases, has a proconvulsant effect. Intraperitoneal imipramine treatment increases norepinephrine level, and to a lesser extent, dopamine and serotonin in brain dialysate from rats. We sought to determine the effects of imipramine on pilocarpine-induced seizures in the immature rats. METHODS: Right and left cortical and hippocampal electrodes were placed in 10~15 day old Sprague-Dawley rats. The following day 3-hour video EEG recordings were obtained to monitor electrographic seizures and status epilepticus induced by intraperitoneal injection of high dose(200mg/kg : HD) and low dose (75mg/kg : LD) pilocarpine. A first group of rats received HD pilocarpine alone(n=25), or pretreatment with 0.5(n=6). 1(n=6), 2(n=6), 5(n=6), or 10(n=11)mg/kg of imipramine. A second group of rats received LD pilocarpine alone(n=6) or pretreatment with 2mg/kg of imipramine (n=6). Data were analyzed using the Fisher exact test. RESULTS: Treatment with HD pilocarpine alone resulted in electrographic seizures in 76%(n=19) and status epilepticus in 44%(n=11). In the HD pilocarpine group, status epilepticus was seen in 100% of rats pretreated 1, 2, and 5mg/kg imipramine. 90.9% of the rats pretreated with 10mg/kg of imipramine developed seizures and 36.4% developed status epilepticus. Imipramine at 0.5, 1,2, and 5mg/kg increased the incidence of seizures and status epilepticus in the HD pilocarpine group, but the incidence was statistically significant only for status epilepticus(p<0.05). The latency for the occurrence of first status epilepticus was 12.3+/-1.3 min in the HD pilocarpine alone group, and it was 21+/-3.8 min and 25.3+/-5.7 min in the group pretreated with 5 and 10mg/kg imipramine, respectively(p<0.02). Treatment with LD pilocarpine alone resulted in seizures in 50% and status epilepticus in 16.7%. In the LD pilocarpine group with imipramine 2mg/kg pretreatment, 83.3% had seizures and status epilepticus. Imipramine at 2mg/kg increased the incidence of seizures and status epilepticus in the LD pilocarpine group, but the increase was statistically significant only for status epilepticus(p<0.05). CONCLUSION: Imipramine exhibits a proconvulsant effect at low doses and an anticonvulsant effect at high doses in the pilocarpine seizure model in immature rats. There may be a dose-dependent effect on monoaminergic receptors which results in increased neuronal excitability.
Animals ; Brain ; Dopamine ; Electrodes ; Electroencephalography ; Imipramine* ; Incidence ; Injections, Intraperitoneal ; Neurons ; Norepinephrine ; Pilocarpine ; Rats* ; Rats, Sprague-Dawley ; Seizures* ; Serotonin ; Status Epilepticus

No abstract available.
Asthma* ; Immunotherapy*

No abstract available.
Epilepsy*

Osteogenesis imperfecta is a heterogenous group of inherited disorder, which has abnormalities in the synthesis of collagen. Among variable clinical manifestations, orthopaedic clinical features are laxity of ligament and deformities arising from frequent fractures or angulation due to skeletal fragility. We had managed 4 cases of osteogensis imperfecta, which were belonged to type IV-B in two cases, type I-A in one, and type I-B in remained one by Sillence classification and they had suffered from recent fractures or deformities due to previous fractures in the long bones of the lower extremities. These patients had been treated with various types of intramedullary nails, such as Bailey-Dubow extensile rod. Rush rod and Kuntcher nail, and the result of intramedullary nailing lead to improvement of walking ability.
Classification ; Collagen ; Congenital Abnormalities ; Fracture Fixation, Intramedullary ; Humans ; Ligaments ; Lower Extremity ; Osteogenesis Imperfecta ; Osteogenesis ; Walking

No abstract available.
Burns* ; Hand* ; Humans ; Infant*

Generalized eruptive histiocytoma is a papular, nonlipidic, self-healing histiocytosis affecting mainly adults. Clinically the disease is characterized by an asymptomatic, widespread, frequently symmetrical eruption of numerous dark red or bluish red papules. In adults the lesions are symmetrically distributed and may involve mucous membranes, while in children the lesions are irregularly scattered over the entire body and mucous membranes spare. We herein report a case of generalized eruptive histiocytoma developed in a 3-year-old boy. He was seen with brownish, flat papules on the face, trunk and both proximal portions of extremities. Histological and immunohistochemical investigation revealed monomorphous infiltration of S-100, CD1a negative, lysozyme positive histiocytes in the upper dermis. Neither cells with foamy cytoplasm nor giant cells was found. He did not show any disease progression without treatment, so now in regular follow-up for exaggeration.
Adult ; Child ; Child, Preschool ; Cytoplasm ; Dermis ; Disease Progression ; Extremities ; Follow-Up Studies ; Giant Cells ; Histiocytes ; Histiocytoma* ; Histiocytosis ; Humans ; Male ; Mucous Membrane ; Muramidase

No abstract available.

This study was attempted to reveal the clinical and electroencephalographic findings in children who were experienced febrile convulsion and epilepsy. The subjects were 226 cases, under 15 years of age, who visited with convulsive disorders to the St. Mary's hospital between Jan. 1977 and Aug. 1978. Of these, 45 cases were febrile convulsion and 123 cases were epilepsies. The results were as follows : 1. The major cause of convulsions, in order of frequency, were epilepsy (54.4%), febrile convulsion (19.9%), meningitis and encephalitis (11.5%), cerebral palsy (4.0%), diarrhea (3.1%) and tetany (1.3%). 2. The leading cause of convulsions were different according to age : Before the age of 3, febrile convulsion, meningitis and encephalitis were common cause of convulsions and epilepsy was common after that age. 3. 68.8% of first febrile convulsion observed during the first 2 years of age. Male to female ratio was 3:2. 4. The EEG findings, of 24 cases with reccurent febrile convulsions, were normal in 29.9%, borderline in 8.3% and abnormal in 62.5% of them. The incidence of abnormal EEG findings were more frequent in the older children than in the younger ones : Of the 10 cases less than 3 years old, the chance of abnormal EEG findings was noted in 2 cases, compared to 13 cases of 14 cases more than 3 years old. This result suggest that the incidence of abnormal EEG in reccurent febrile convulsion seems to be correlated with the preexisting numbers of convulsive attacks. The findings of abnormal EEG were sporadic spike (40.0%), high voltage slow wave (20.0%), multiple spike (20.0%), slow wave dysrhythmia (13.3%) and spike and wave complex (6.7%). 5. The clinical type of epilepsies, in order of frequency, were grand mal (45.5%), minor motor seizure (17.1%), focal seizure (9.8%), petit mal absence (8.1%) and psychomotor seizure (4.9%). The major abnormal EEG finding in grand mal and focal seizure was asymmetric and focal slow wave, and it was found in 33.9% and 58.3% of their traces respectively. In psychomotor seizure, spike or sharp wave was noted in 66.7%, and in petit mal absence and minor seizure, spike and wave complex were noted 100.0% and 33.3% of them.
Cerebral Palsy ; Child ; Child, Preschool ; Diarrhea ; Electroencephalography ; Encephalitis ; Epilepsy* ; Female ; Humans ; Incidence ; Male ; Meningitis ; Seizures ; Seizures, Febrile* ; Tetany