1.Effect of Ischemic Preconditioning on Catecholamine Release from the Isolated, Ischemic Reperfused Hearts of Rats.
Jong Wan PARK ; Young Hoon KIM ; Myung Suk KIM
Korean Circulation Journal 1995;25(6):1217-1224
BACKGROUND: Ischemic preconditioning reduces the infarct size and the severity of arrhythmia in a post-ischemic reperfused heart although the detailed mechanism is unknown. In the ischemic heart, a large amount of catecholamine is released from the adrenergic nerve terminal and this aggravates cell destruction and arrhythmia. In this study, the possibility for ischemic preconditioning to inhibit the release of endogenous catecholamine from the ischemic heart was tested to investigate the probable cardioprotective mechanism of ischemic preconditioning. METHODS: In the isolated, Langendorff perfused rat hearts, we observed the protective effect of ischemic preconditioning against post-ischemic reperfusion injury, and measured the amount of catecholamine released into coronary effuent. In addition, we observed the effect of catecholamine depletion on reperfusion injury in non-preconditioned and preconditioned hearts. RESULTS: During the reperfusion(20min) after ischemia(30min), the cardiac function was markedly depressed with the development of severe contracture. In the heart preconditioned by three sequential episodes of 5min ischemia and 5min reperfusion, the reperfusion contracture decreased significantly and the cardiac function was almost recovered to normal after 20min reperfusion. The release of lactate dehydrogenase was also decreased in the preconditioned heart. The release of endogenous catecholamine was abruptly increased immediately after the reperfusion and the release was exponentially decreased throughout the reperfusion period. THe pattern of catecholamine release was much different from that of lactate dehydrogenase release. In the preconditioned heart, the release was significantly decreased to about half of that in non-preconditioned t\heart. Endogenous catecholamine depletion by reserpine treatment did not affect the post-ischemic functional recovery in both non-preconditioned and preconditioned hearts. CONCLUSION: It is suggested from these results that ischemic preconditioning inhibis the release of endogenous catecholamine during ischemic period, which may be partly related to cardioporotective effect of preconditioning in ischemic and reperfused heart.
Animals
;
Arrhythmias, Cardiac
;
Contracture
;
Heart*
;
Ischemia
;
Ischemic Preconditioning*
;
L-Lactate Dehydrogenase
;
Rats*
;
Reperfusion
;
Reperfusion Injury
;
Reserpine
2.A Case of Eosinophilic Gastroenteritis Associsted with Protein - losing Enteropathy.
Jong Jae PARK ; Hoon Ki PARK ; Suk Kee PAIK ; Jung Lyae HYUN ; Tong Jhin KIM
Korean Journal of Gastrointestinal Endoscopy 1994;14(1):74-81
Eosinophilic gastroenteritis is characterized by peripheral eosinophilia, eosinophilic infiltration of the bowel and gastrointestinal symptoms. The disease may affect any area of the gastrointestinal tract. Various manifestations are present, corresponding to the predominant layer of the eosinophilic infiltration. We experienced a case of eosinophilic gastroenteritis presenting as protein-losing enteropathy in a 36-year old male patient with acute upper abdominal pain and watery diarrhea. He showed peripheral eosinophilia, hypoalbuminemia, penile., thigh and ankle edema. The patient was treated with oral corticosteroid, and supportive care such as parenteral albumin, fluid and electrolytes. Almost all symptoms resloved within 1 week. The present report concerns a case of eosinophilic gastroenteritis associated with protein loss. Cases, of eosinophilic gastroenteritis with protein-losing enteropathy are not common. To our knowledge, no case has reported in Korea. So we report this case with brief review of the literature.
Abdominal Pain
;
Adult
;
Ankle
;
Diarrhea
;
Edema
;
Electrolytes
;
Eosinophilia
;
Eosinophils*
;
Gastroenteritis*
;
Gastrointestinal Tract
;
Humans
;
Hypoalbuminemia
;
Korea
;
Male
;
Protein-Losing Enteropathies
;
Thigh
3.Expression of Osteopontin in Osteoclast.
Jae Suk CHANG ; Jong Hoon PARK ; Yong Gu PARK ; Jeong Hwa KIM
Journal of Korean Orthopaedic Research Society 1999;2(2):132-138
PURPOSE: This study was aimed to determine the relationship between osteopontin(OPN) and osteoclast, especially focused on whether ostecolast could produce osteopontin or not. MATERIALS AND METHODS: Osteoclasts were isolated from the giant cell tumor of proximal tibia and seeded on the 13 mm round cover slip resided in 24 multi-well plates for culture. After 2 days, osteclasts on the cover slip were fixed with cold acetone for 3 minutes and immunocytochemistry was done with rabbit osteopontin antibody. For in situ RT-PCR, osteoclasts on the cover-slips were fixed with 4% paraformaldehyde for 4 hours and were treated to pepsin. PR-PCR was done and the PCR producst were stained with anti-digoxigenin-AP. RESULTS: Osteopontins were found on the surface of the osteoclast by immunocytochemistry, and intense osteopontin mRNAs were found by in situ RT-PCR. CONCLUSION: We have identified that osteoclast could synthesize the osteopontin, and confirmed that in situ RT-PCR was a very useful method in expressing small amount of mRNA in case of mixed cell culture. Further study was needed to identify the action of the osteopontin produced by the osteoclast.
Acetone
;
Cell Culture Techniques
;
Giant Cell Tumors
;
Immunohistochemistry
;
Osteoclasts*
;
Osteopontin*
;
Pepsin A
;
Polymerase Chain Reaction
;
RNA, Messenger
;
Tibia
5.DNA Quantitation by Image Cytometry in Bone Tumor
Kwang Suk LEE ; Sang Won PARK ; In Sun KIM ; Jong Hoon PARK ; Gyou Hyouk LEE
The Journal of the Korean Orthopaedic Association 1995;30(6):1573-1578
There are substantial evidence suggesting that DNA content of tumors may provide the prognostic information with independent significances. With the advent of computer and video technology, image analysis becomes a practical method of measuring DNA that also makes it possible to classify cells. Among the patients who were operated and diagnosed as bone tumor at Department of Orthopedic Surgery, Korea University Hospital, from March 1992 to March 1995, we evaluated 19 cases by image cytometry and studied them. Among 19 case, 4 cases were fibrous dysplasia, 2 cases chondroma, 3 cases osteosarcoma, 2 cases chondrosarcoma, and 8 cases were metastatic bone tumors. Total benign tumors were 6 cases and malignant tumors were 13 cases. All benign tumors were diploid and all malignant tumors but one metastatic tumor were aneuploid. As a result, DNA quantitation by image analysis is effective in the differential diagnosis of malignancy in bone tumor. It seems that DNA quantitation will be used on the evaluation of tumor staging and prognosis by further clinical study.
Aneuploidy
;
Chondroma
;
Chondrosarcoma
;
Clinical Study
;
Diagnosis, Differential
;
Diploidy
;
DNA
;
Humans
;
Image Cytometry
;
Korea
;
Methods
;
Neoplasm Staging
;
Orthopedics
;
Osteosarcoma
;
Prognosis
6.Comparative study of the Characteristics of Collagen Synthesis by Cultured Fibroblasts in the Fetus and Adult.
Dong Kyun RAH ; Tai Suk ROH ; Beyoung Yun PARK ; Kwang Hoon LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1999;26(3):377-382
With the development of antenatal diagnostic tools such as ultrasonography, some congenital anomalies or diseases can be detected in early fetal life. Routine serial antenatal check-ups have made it possible to predict the prognosis of these problems, and a few life-threatening single anatomic malformations have been treated by open fetal surgery. The experience of fetal surgery revealed that the human fetus appears to heal without any scarring. In contrast to adult animals, the response to tissue injury in the fetus is conspiciously devoid of acute inflammation. Indeed, the absence of neutrophils is perhaps the most consistent observation in fetal wounds and seems to be followed by absent or scanty fibroblast infiltration, which results in healing with sparse and well organized collagen deposition. Actually, the amount and quality of the collagen deposition were decided by the fibroblasts which infiltrated the wound. It is well known that fetal wounds have sparse collagen deposition, however, the mechanisms are still unclear. This study was designed to evaluate the role of fibroblast activity in the differences of the scar formation between the fetus and neonate. Fibroblast activity such as the cell growth rate, the amount of collagen synthesis and the synthesized collagen types of fetus(IUP 18-22 weeks) was compared with that of neonate. The amount of collagen synthesis was measured by H-proline uptake and the amount of collagen type III was measured by Western blot using antihuman procollagen type III. The cell growth rate as determined by cell proliferation from the initial cell count of 5x10(5) to cell confluence was 3.6 x 10(6) in the fetal fibroblasts compared to 2.5x10(6) in neonatal fibroblasts. Fetal fibroblast synthesize 16.9 x 10(4) cpm of collagen and neonatal fibroblasts synthesize 2.7 x 10(4) cpm of collagen. The synthesized amount of type III collagen was 2.1x10(4) ug/ml, and 1.5x10(4) ug/ml by fetal and neonatal fibroblasts, respectively. In conclusion, fetal fibroblasts grow faster and synthesize a smaller amount of collagen, but produce more type III collagen than neonatal fibroblasts.
Adult*
;
Animals
;
Blotting, Western
;
Cell Count
;
Cell Proliferation
;
Cicatrix
;
Collagen Type III
;
Collagen*
;
Fetus*
;
Fibroblasts*
;
Humans
;
Infant, Newborn
;
Inflammation
;
Neutrophils
;
Prognosis
;
Ultrasonography
;
Wounds and Injuries
7.Experimental Reproduction of Skin Lesions on Polymorphous Light Eruption.
Kyung Jeh SUNG ; Jeung Hoon LEE ; Kyung Chan PARK ; Won Suk KIM
Korean Journal of Dermatology 1982;20(1):29-33
It has been proposed that action spectrum in polymorphous light eruption lies predominantly in the sunburn range, but reaches through the long-wave ultraviolet (UV) into visible part of the spectrum. We examined the efficacy of UUB, UVA and visible light in reproducing skin lesions, and found that the UVA was most suitable in provoking the typical skin lesions in our 3 cases with polymorphous light eruption, Plewig and his associates reproduced the clinical lesions with megadoses of UVA,(60J/cm2); in our experiment, 5 to 10 J/cm2 was sufficient. The reason for this discrepancy is not clear but the method of phototest, light source and energy, the different susceptibility in different races and other factors may influence the phototest results.
8.Immunogenicity from polio/hepatitis B chimeric virus.
Tae Wook HAN ; Ree Ann YOO ; Suk Hoon HA ; Wan Je PARK ; Hyun Su KIM
Journal of the Korean Society of Virology 1992;22(2):111-117
No abstract available.
9.Reconstruction with free flap in electrical hand injury.
Byung Sun PARK ; Chul Hoon CHUNG ; Dong Lark LEE ; Suk Joon OH
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1993;20(2):417-422
No abstract available.
Free Tissue Flaps*
;
Hand Injuries*
;
Hand*
10.Chlamydial infection in women with tubal pregnancy.
Woo Sik LEE ; Dong Hoon HWANG ; Byung Suk LEE ; Ki Hyun PARK ; Chan Ho SONG
Korean Journal of Obstetrics and Gynecology 1993;36(4):534-542
No abstract available.
Female
;
Humans
;
Pregnancy
;
Pregnancy, Tubal*