OBJECTIVE: To investigate the expression of fibroblast growth factor-2 (FGF-2) and osteopontin (OPN) in non-small cell lung cancer (NSCLC) tissues and analyze the correlation between FGF-2 and OPN. METHODS: Immunohistochemical SP method was used to detect the expression of FGF-2 and OPN in 76 patients with NSCLC and 15 normal lung tissues. The effect of FGF-2 on OPN expression at mRNA and protein level in A549 cell was examined by RT-PCR and Western blot. RESULTS: The positive expression of FGF-2 (65.8%) and OPN (60.5%) in the NSCLC tissues was significantly higher than that in the normal lung tissues (13.3% and 0, respectively ) (P<0.01). The expression of FGF-2 and OPN was closely related to TNM stages and the lymph node metastasis (all Ps<0.01), but not to histological types, sex, and age of NSCLC patients (all Ps>0.05).A positive correlation was found between the expression of FGF-2 and OPN in NSCLC (r=0.552,P<0.01). The expression of OPN protein and mRNA was up-regulated by FGF-2 in A549 cells. CONCLUSION The overexpression of FGF-2 and OPN is related to the metastasis and invasion of NSCLC.FGF-2 may promote the metastasis and invasion of NSCLC depending on the upregulation of OPN expression.
Adult ; Aged ; Carcinoma, Non-Small-Cell Lung ; metabolism ; pathology ; Female ; Fibroblast Growth Factor 2 ; genetics ; metabolism ; Humans ; Lung Neoplasms ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Metastasis ; Osteopontin ; genetics ; metabolism ; RNA, Messenger ; genetics ; metabolism

Objective:To explore the multiple mediation effects of the positive and negative affect between emotional intelligence and life satisfaction in military personnel.Methods:Totally 967 male military personnel aged 17 to 34 years were investigated with the Schutte's Emotional Intelligence Scale (SSEIS),the Positive Affect and Negative Affect Scale (PANAS) and Life Satisfaction Index A (LSIA).The relationship among these variables was analyzed by using path analysis and Bootstrap test.Results:Totally 68.7％ military personnel were satisfied with their lives.Path analysis showed that emotional intelligence had indirect effects on life satisfaction through the mediation effects of positive affect and negative affect (x2/df=8.88,GFI =0.98,AGFI =0.94,NFI =0.98,IFI =0.98,TLI =0.95,CFI =0.98,RMSEA =0.09).Bootstrap test showed that the mediational path through positive affect and negative affect with the effect size were 31.5％ and 14.8％,and the total mediational effect size was 46.3％.Conclusion:It suggests that positive affect and negative affect may have mediating effects on the relationship between emotional intelligence and life satisfaction among military personnel.