Objective:To investigate the effect of cilostazol on erectile function in diabetic rat model.Methods:Diabetes were induced in 50 male adult sprague-dawley(SD) rats by an intraperitoneal injection of streptozotocin.The DM rats were randomly divided into diabetic group(DM group )and cilostazol therapy group(CI group) 8 weeks after the injection(another 10 normal SD rats as control,C group).The rats in each group were treated with cilostazol20 mg/(kg?d) or NS through a gastric perfusion for 8 weeks.Then the erectile function of all these rats was evaluated by apomorphine.Motor nerve conduction velocity of sciatic nerve(MNCV) was evaluated by electrophysiology method.Their penis tissues were harvested to measure the level of nitric oxide synthase(NOS) by colorimetric method,and the penile cell apoptosis of smooth muscle was analyzed by TUNEL assay.Results:The erectile function,MNCV and NOS level in CI group significantly increased,while the apoptosis ratio significantly decreased(P

Aim To study the effects of ginsenoside-Ro on cell proliferation and cytokine production in murine splenocytes. Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3 H] thymidine incorporation assay. Effects of ginsenoside-Ro on the production of cytokines interleukin-2 (IL-2), interferon-γ (IFN-γ) and interleukin-4 (IL-4) from murine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of Th1 cytokine IFN-γ and Th2cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis.Results Ginsenoside-Ro showed no mitogenic effect on unstimulated murine splenocytes. It enhanced the proliferation of Con A-induced murine splenocytes and the production of IL-2 at concentrations of 1 - 10decreased the production and expression of Th1 cytokine IFN-γ in Con A-induced murine splenocytes at regulating the production and expression of Th1/Th2 cytokines in murine splenocytes.