This article puts forwards the main measures to reasonably select the teaching materials and promote its construction,according to the characteristic and task of teaching reform during up-to-date period.

Objective To reviewing the developmental history and to make an overall inquiry of the status quo of critical care medicine(CCM)in China,in order to make suggestions regarding the future development of this subspecialty in this country,particularly in the army forces.Methods Relevant articles regarding this subspecialty were retrieved from the published literature,and important events as well as advances in this field were reviewed to illustrate the history of establishment of intensive care unit(ICU),important events in the development,and academic advances in this subspecialty in this country and especially in the army forces.Results The review illustrated that this subspecialty of medical science had made a big stride in the past thirty years,and it has become one of the major forces in the medical arena in this country.The change in stratification of patients as a whole,further fragmentation of medical discipline,experiences obtained from participation in the care of mass casualties in multiple disasters,concerted efforts made by specialists devoted to this field of medicine,as well as strong support from the administration had given tremendous impetus to an upsurge of this subspecialty. In this army forces,this subspecialty had advanced in pace with that in the whole country.The inauguration of Military Association of CCM had further pushed forward the science and arts of CCM in the armed forces.Nevertheless,there are still many important problems in this field to be investigated.Conclusions The administration should give more support and advices for development of this important medical subspecialty in the armed forces,in order to further raise the standard of the arts and science of this branch of medical science to face any challenge in the care of serious illnesses and injuries encountered both in peace time and in combats.It is also important to propagate the perception of CCM in the medical establishments of grassroots level.

Medical simulation education is a developing trend for expanding clinical teaching.It is a systematic engineering including teaching design,content,method and teaching arrangement.The paper introduced the experience of introducing clinical simulation model to improving clinic teaching level in the common medical college.

Objective To compare the clinical values of 16-slice spiral coroputed tomography(CT)and matrix metalloproteinases-9(MMP-9)in diagnosis of invasion and metastasis in human esophageal cancer.Methods Thirty-eight patients with esophageal cancer were detected with spiral CT,and their tumor tissues were detected for expression of MMP-9.Results 12 cases were diagnosed for vicinitic tissues damages by histopathological examination,while 11 cases were regarded to be damaged by CT and 13 cases were done according to MMP-9 determination;as to metastasis,there were 13 casese by histopathological examination,12 cases by CT and 16 cascs by MMP-9 determination.No statistical difference were observed in diagnosis of vicinitic tissues damages and metastasis by the three methods in positive rates,sensitivity,accuracy,specificity and diagnosis accordance rates(P＞0.05).Conclusion It is the important method for spiral CT in diagnosis of esophageal cancer,which could not be replaced by others in judgement of invasion and metastasis.

Objective To understand hot-spot of preventive medicine research at the beginning of the 21st century.Methods To analyze all the key words in the articles from four highly influential comprehensive preventive medical journals from 2001 to 2006 through word frequency analysis.Results In research population,children have always been the focus of attention,and more attention was paid to young people and college students in 2006.In research methods,the epidemiological and the molecular biological methods have been the main preventive medicine research ones.As regards research content,risk factors have been mainly studied;hypertension and obesity has been focused on,and diabetes and AIDS have been paid more attention to since 2003;Lead on trace elements has received more attention gradually.Conclusions Close attention should be given to the research of preventive medicine in the rural areas.

The influence of sodium selenite on the replication of Coxsackie B5 virus in monkey kidney cells was studied. When the host cells were cultured in a medium containing 0.05 or 0.5 ug/ml sodium selenite and reproduced continually to 3-10 generations, the titer of Coxsackie B5 virus in cell suspensions was markedly reduced and accompanied with the obvious increase of the surviving rate of the host cells (p

The effect of selenium on the synthesis of nucleic acids in human am-nion cells and monkey kidey cells was studied by the techniques of cell culture and radioisotope assay. The cells were cultured in a medium containing varied dosages of sodium selenite for 24 hours at 37℃ in a CO2 incubator (5% CO2), then 3H-labeled precursors of DNA and RNA were added. The synthetic activity of DNA and RNA was determined by assaying the incorporation amount of radioisotope. The result suggested that selenium can promote intracellular DNA and RNA synthesis and may be essential to cell growth.

Objective To explore the clinical value of 16-slice spiral CT and its post-processing technics in diagnosis of colorectal carcinoma.Methods CT characteristics,surgery and pathological data of forty-three patients with colorectal carcinoma were analyzed retrospectively.Results The accurate diagnosis rate using 16-slice spiral CT was 100%,a total of forty-five focus were observed by CT and its post-processing pictures.It is safe to detect colorectal carcinoma using spiral CT without wounds.Further more,tumors and its growth towards enteric cavity,wall thichness,damaged surroundding tissues were all observed clearly as well as metastasize.Conclusion 16-slice spiral CT and its post-processing technics is effective in diagnosis of colorectal carcinoma,which should be used in clinical experiences.

Since the emergence of the new syndrome, termed multiple system organ failure (MSOF), about two decades age, it has aroused an outburst of enthusiasm in clinical and experimental studies among many clinicians and investigators both abroad and at home. Nevertheless, it has also created much confusion in the concept as well as the diagnosis of the syndrome, mainly because the present term does not clearly differentiate this syndrome from some other irrelevant clinical conditions, such as senile dysfunction of multiple organs, agonal state etc. As it has been documented to occur almost as a rule after a septic state and also under the intricate interaction of many humoral and cellular mediators, we suggest that the diagnosis of MSOF should at least include two concurrent conditions, i. e. septic response and organ failure.Inasmuch as the present diagnosis of MSOF designates only the terminal state of a series of pat-hophysiological process, namely systemic inflammatory response---sepsis--septic syndrome--MSOF,it is not conducive to an early diagnosis, which is of utmost importance in the prevention and early treatment of this frequently fatal syndrome. In the light of current investigations, we propose that the name of the syndrome be changed to "sepsis and organ dysfunction" or "mediator injury of organs*. Our humble opinion welcomes further comments and discussion.

Objective To investigate the inhibitory effect of the mammalian target of rapamycin (mTOR) inhibitor, sirolimus on expression of hypoxia-inducible factor (HIF)1? protein and growth of ovarian carcinoma in an athymic mouse xenogeneic transplant model of ovarian cancer. Methods Four groups of female nude mice were inoculated subcutaneously with SKOV3 cells. After inoculation, mice were treated with saline, rapamycin alone, paclitaxel alone and sirolimus+paclitaxel. In each tumor protein expressions of HIF-1?,bcl-2 and apoptosis were determined by immunohistochemistry and RT-PCR. Results In sirolimus and sirolimus +paclitaxel groups protein expression of HIF-1? was inhibited. Tumor burden in rapamycin alone, sirolimus +paclitaxel, and paclitaxel alone was reduced by 47.9%( P 0.05) respectively compared with controls. Cell apoptosis inder in sirolimus alone(36), sirolimus +paclitaxel(40), paclitaxel alone(22),increased compared with control(15), while expression of bcl-2 decreased compared with control. Conclusion Sirolimus inhibited protein expression of HIF-1?, increased tumor apoptosis and decreased tumor growth.