This article puts forwards the main measures to reasonably select the teaching materials and promote its construction,according to the characteristic and task of teaching reform during up-to-date period.

Medical simulation education is a developing trend for expanding clinical teaching.It is a systematic engineering including teaching design,content,method and teaching arrangement.The paper introduced the experience of introducing clinical simulation model to improving clinic teaching level in the common medical college.

Objective To reviewing the developmental history and to make an overall inquiry of the status quo of critical care medicine(CCM)in China,in order to make suggestions regarding the future development of this subspecialty in this country,particularly in the army forces.Methods Relevant articles regarding this subspecialty were retrieved from the published literature,and important events as well as advances in this field were reviewed to illustrate the history of establishment of intensive care unit(ICU),important events in the development,and academic advances in this subspecialty in this country and especially in the army forces.Results The review illustrated that this subspecialty of medical science had made a big stride in the past thirty years,and it has become one of the major forces in the medical arena in this country.The change in stratification of patients as a whole,further fragmentation of medical discipline,experiences obtained from participation in the care of mass casualties in multiple disasters,concerted efforts made by specialists devoted to this field of medicine,as well as strong support from the administration had given tremendous impetus to an upsurge of this subspecialty. In this army forces,this subspecialty had advanced in pace with that in the whole country.The inauguration of Military Association of CCM had further pushed forward the science and arts of CCM in the armed forces.Nevertheless,there are still many important problems in this field to be investigated.Conclusions The administration should give more support and advices for development of this important medical subspecialty in the armed forces,in order to further raise the standard of the arts and science of this branch of medical science to face any challenge in the care of serious illnesses and injuries encountered both in peace time and in combats.It is also important to propagate the perception of CCM in the medical establishments of grassroots level.

BACKGROUND:Studies have shown that as a regulator of bone marrow functionerythropoietinis a glycoprotein that controls the development of the central nervous system and has neurotrophic and neuroprotective potential. Therefore, transplantation of human amniotic mesenchymal stem cels geneticaly modified by human erythropoietin is a new choice for brain injury treatment.
OBJECTIVE:To observe the effect of transplantation of human amniotic mesenchymal stem cels geneticaly modified byhuman erythropoietin on the functional recovery from brain injury in rats.
METHODS:Eukaryotic expression plasmid pcDNA3.1 carrying erythropoietin was successfuly constructed and transferred into amniotic mesenchymal stem cels culturedin vitro. Expression of erythropoietin was detected using western blot assay before and after transfection. Rat models of middle cerebral arterial occlusion was made and given transplantation of transfected amniotic mesenchymal stem celsviathe tail vein (transfection group). Additionaly, model and simple cel transplantation groups were set in a comparative study.
RESULTS AND CONCLUSION:Findings from western blot detection showed that transfected cels could express human erythropoietin. Compared with the other groups, modified neurologic severity scores, growth-associated protein 43 and aquaporin 9 at mRNA and protein levels were al decreased significantly in the transfection group. Furthermore, the number of cels positive for CM-Dil was highest in the transfectiongroup, folowed by simple cel transplantation group, and lowest in the model group (alP<0.05). Overal findings from this study show that human erythropoietin-modified human amniotic mesenchymal stem cel transplantation promotes neurologic recoveryfrom brain injury through eliciting a reduction in growth-associated protein 43 and aquaporin 9 at mRNA and protein levels as wel as inhibiting cel apoptosis.

AIM: To detect the expression of WNT5B in normal breast epithelial cells and different breast cancer cell lines, and to investigate the effects of WNT5B over-expression on the viability and apoptosis of human breast cell line MCF-7.METHODS: The mRNA expression of WNT5B was detected by RT-PCR in different breast cancer cells. MCF-7 cells were transfected with plasmid pcDNA3.1 /WNT5B or pcDNA3.1, and the expression of WNT5B at mRNA and protein levels was examined in the 2 groups by real-time PCR and Western blotting, respectively.Subsequently, the chan-ges of cell viability and cell apoptosis were analyzed by CCK-8 assay and flow cytometry, respectively.RESULTS: The ex-pression of WNT5B in the breast cancer cell lines was lower than that in MCF10A cells.The WNT5B expression in the MCF-7 cells in experimental group was significantly higher than that in vector group (P <0.05).However, the cell viabili-ty in experimental group decreased significantly as compared with vector group (P <0.05).The number of the cells in S-phase obviously increased, while the percentage of the cells in G1-phase and G2 /M-phase decreased compared with vector group.The number of apoptotic cells in WNT5B group was significantly higher than that in vector group.CONCLUSION:The expression of WNT5B is decreased in breast cancer cells.WNT5B over-expression significantly inhibits the cell growth and promotes the cell apoptosis in breast cancer MCF7 cells.

In recent years,hepatocellular carcinoma (HCC)has become the second leading cause of cancer-related death worldwide.Due to the insidious onset of this disease,only 20%-30%of the patients with HCC have the opportunity for surgical resection or liver transplan-tation.At present,interventional therapy has become the first choice for patients with intermediate-stage HCC and some patients with late-stage HCC,and more and more scholars have realized the importance and necessity of comprehensive interventional therapy for HCC.The comprehensive interventional therapy of transcatheter arterial chemoembolization (TACE)in combination with various methods (TACE com-bined with local treatment,TACE sequential surgery,or TACE combined with systemic treatment)provides many therapeutic approaches and achieves good therapeutic effects.However,the indications,timing,and recurrence and metastasis after treatment for such combination ther-apies await further investigation.

Cervical carcinoma is a serious threat to the health of women around the world ,and its inci-dence ranks at the second position after breast cancer in female reproductive system .In addition to oncogene acti-vation and inactivation of tumor suppressor gene ,endogenous and exogenous factors also affect the development of cervical cancer .

Objective To understand hot-spot of preventive medicine research at the beginning of the 21st century.Methods To analyze all the key words in the articles from four highly influential comprehensive preventive medical journals from 2001 to 2006 through word frequency analysis.Results In research population,children have always been the focus of attention,and more attention was paid to young people and college students in 2006.In research methods,the epidemiological and the molecular biological methods have been the main preventive medicine research ones.As regards research content,risk factors have been mainly studied;hypertension and obesity has been focused on,and diabetes and AIDS have been paid more attention to since 2003;Lead on trace elements has received more attention gradually.Conclusions Close attention should be given to the research of preventive medicine in the rural areas.

The influence of sodium selenite on the replication of Coxsackie B5 virus in monkey kidney cells was studied. When the host cells were cultured in a medium containing 0.05 or 0.5 ug/ml sodium selenite and reproduced continually to 3-10 generations, the titer of Coxsackie B5 virus in cell suspensions was markedly reduced and accompanied with the obvious increase of the surviving rate of the host cells (p

The effect of selenium on the synthesis of nucleic acids in human am-nion cells and monkey kidey cells was studied by the techniques of cell culture and radioisotope assay. The cells were cultured in a medium containing varied dosages of sodium selenite for 24 hours at 37℃ in a CO2 incubator (5% CO2), then 3H-labeled precursors of DNA and RNA were added. The synthetic activity of DNA and RNA was determined by assaying the incorporation amount of radioisotope. The result suggested that selenium can promote intracellular DNA and RNA synthesis and may be essential to cell growth.