Objective To reviewing the developmental history and to make an overall inquiry of the status quo of critical care medicine(CCM)in China,in order to make suggestions regarding the future development of this subspecialty in this country,particularly in the army forces.Methods Relevant articles regarding this subspecialty were retrieved from the published literature,and important events as well as advances in this field were reviewed to illustrate the history of establishment of intensive care unit(ICU),important events in the development,and academic advances in this subspecialty in this country and especially in the army forces.Results The review illustrated that this subspecialty of medical science had made a big stride in the past thirty years,and it has become one of the major forces in the medical arena in this country.The change in stratification of patients as a whole,further fragmentation of medical discipline,experiences obtained from participation in the care of mass casualties in multiple disasters,concerted efforts made by specialists devoted to this field of medicine,as well as strong support from the administration had given tremendous impetus to an upsurge of this subspecialty. In this army forces,this subspecialty had advanced in pace with that in the whole country.The inauguration of Military Association of CCM had further pushed forward the science and arts of CCM in the armed forces.Nevertheless,there are still many important problems in this field to be investigated.Conclusions The administration should give more support and advices for development of this important medical subspecialty in the armed forces,in order to further raise the standard of the arts and science of this branch of medical science to face any challenge in the care of serious illnesses and injuries encountered both in peace time and in combats.It is also important to propagate the perception of CCM in the medical establishments of grassroots level.

Medical simulation education is a developing trend for expanding clinical teaching.It is a systematic engineering including teaching design,content,method and teaching arrangement.The paper introduced the experience of introducing clinical simulation model to improving clinic teaching level in the common medical college.

This article puts forwards the main measures to reasonably select the teaching materials and promote its construction,according to the characteristic and task of teaching reform during up-to-date period.

Objective To explore the clinical value of 16-slice spiral CT and its post-processing technics in diagnosis of colorectal carcinoma.Methods CT characteristics,surgery and pathological data of forty-three patients with colorectal carcinoma were analyzed retrospectively.Results The accurate diagnosis rate using 16-slice spiral CT was 100%,a total of forty-five focus were observed by CT and its post-processing pictures.It is safe to detect colorectal carcinoma using spiral CT without wounds.Further more,tumors and its growth towards enteric cavity,wall thichness,damaged surroundding tissues were all observed clearly as well as metastasize.Conclusion 16-slice spiral CT and its post-processing technics is effective in diagnosis of colorectal carcinoma,which should be used in clinical experiences.

Objective To compare the clinical values of 16-slice spiral coroputed tomography(CT)and matrix metalloproteinases-9(MMP-9)in diagnosis of invasion and metastasis in human esophageal cancer.Methods Thirty-eight patients with esophageal cancer were detected with spiral CT,and their tumor tissues were detected for expression of MMP-9.Results 12 cases were diagnosed for vicinitic tissues damages by histopathological examination,while 11 cases were regarded to be damaged by CT and 13 cases were done according to MMP-9 determination;as to metastasis,there were 13 casese by histopathological examination,12 cases by CT and 16 cascs by MMP-9 determination.No statistical difference were observed in diagnosis of vicinitic tissues damages and metastasis by the three methods in positive rates,sensitivity,accuracy,specificity and diagnosis accordance rates(P＞0.05).Conclusion It is the important method for spiral CT in diagnosis of esophageal cancer,which could not be replaced by others in judgement of invasion and metastasis.

OBJECTIVE:To establish the UPLC fingerprint for Qinghou liyan granule,and provide reference for its quality control by combining with principal component analysis (PCA). METHODS:UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile(A)-0.1% aqueous phosphoric acid solution(B)(gradient elution)at a flow rate of 0.4 mL/min,the detection wavelength was 280 nm,column temperature was 30℃,and injection volume was 2μL. Using ellag-ic acid as a reference,12 batches of samples were analyzed,Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicinewas used for the similarity analysis and identification of the common peaks,and the PCA was used for common peaks. RESULTS:There were 18 common peaks in the fingerprints of 12 batches of samples,and 6 principal peaks (gallic acid,ellagic acid,naringin,hesperidin,neohesperidin and baicalin) were identified;the similarity degree of 12 batches and reference fingerprints were no less than 0.984. According to PCA,the 18 peaks can be integrated into 3 principal components, with cumulative contribution rate of principal component of 78.277%;baicalin and 16 peaks were the discriminating factors of the fingerprint of Qinghou liyan granule. CONCLUSIONS:The method can provide reference for the quality control of Qinghou liyangranule.

AIM: To detect the expression of WNT5B in normal breast epithelial cells and different breast cancer cell lines, and to investigate the effects of WNT5B over-expression on the viability and apoptosis of human breast cell line MCF-7.METHODS: The mRNA expression of WNT5B was detected by RT-PCR in different breast cancer cells. MCF-7 cells were transfected with plasmid pcDNA3.1 /WNT5B or pcDNA3.1, and the expression of WNT5B at mRNA and protein levels was examined in the 2 groups by real-time PCR and Western blotting, respectively.Subsequently, the chan-ges of cell viability and cell apoptosis were analyzed by CCK-8 assay and flow cytometry, respectively.RESULTS: The ex-pression of WNT5B in the breast cancer cell lines was lower than that in MCF10A cells.The WNT5B expression in the MCF-7 cells in experimental group was significantly higher than that in vector group (P <0.05).However, the cell viabili-ty in experimental group decreased significantly as compared with vector group (P <0.05).The number of the cells in S-phase obviously increased, while the percentage of the cells in G1-phase and G2 /M-phase decreased compared with vector group.The number of apoptotic cells in WNT5B group was significantly higher than that in vector group.CONCLUSION:The expression of WNT5B is decreased in breast cancer cells.WNT5B over-expression significantly inhibits the cell growth and promotes the cell apoptosis in breast cancer MCF7 cells.

In recent years,hepatocellular carcinoma (HCC)has become the second leading cause of cancer-related death worldwide.Due to the insidious onset of this disease,only 20%-30%of the patients with HCC have the opportunity for surgical resection or liver transplan-tation.At present,interventional therapy has become the first choice for patients with intermediate-stage HCC and some patients with late-stage HCC,and more and more scholars have realized the importance and necessity of comprehensive interventional therapy for HCC.The comprehensive interventional therapy of transcatheter arterial chemoembolization (TACE)in combination with various methods (TACE com-bined with local treatment,TACE sequential surgery,or TACE combined with systemic treatment)provides many therapeutic approaches and achieves good therapeutic effects.However,the indications,timing,and recurrence and metastasis after treatment for such combination ther-apies await further investigation.

Cervical carcinoma is a serious threat to the health of women around the world ,and its inci-dence ranks at the second position after breast cancer in female reproductive system .In addition to oncogene acti-vation and inactivation of tumor suppressor gene ,endogenous and exogenous factors also affect the development of cervical cancer .

BACKGROUND:Studies have shown that as a regulator of bone marrow functionerythropoietinis a glycoprotein that controls the development of the central nervous system and has neurotrophic and neuroprotective potential. Therefore, transplantation of human amniotic mesenchymal stem cels geneticaly modified by human erythropoietin is a new choice for brain injury treatment.
OBJECTIVE:To observe the effect of transplantation of human amniotic mesenchymal stem cels geneticaly modified byhuman erythropoietin on the functional recovery from brain injury in rats.
METHODS:Eukaryotic expression plasmid pcDNA3.1 carrying erythropoietin was successfuly constructed and transferred into amniotic mesenchymal stem cels culturedin vitro. Expression of erythropoietin was detected using western blot assay before and after transfection. Rat models of middle cerebral arterial occlusion was made and given transplantation of transfected amniotic mesenchymal stem celsviathe tail vein (transfection group). Additionaly, model and simple cel transplantation groups were set in a comparative study.
RESULTS AND CONCLUSION:Findings from western blot detection showed that transfected cels could express human erythropoietin. Compared with the other groups, modified neurologic severity scores, growth-associated protein 43 and aquaporin 9 at mRNA and protein levels were al decreased significantly in the transfection group. Furthermore, the number of cels positive for CM-Dil was highest in the transfectiongroup, folowed by simple cel transplantation group, and lowest in the model group (alP<0.05). Overal findings from this study show that human erythropoietin-modified human amniotic mesenchymal stem cel transplantation promotes neurologic recoveryfrom brain injury through eliciting a reduction in growth-associated protein 43 and aquaporin 9 at mRNA and protein levels as wel as inhibiting cel apoptosis.