Objective:To investigate the regulatory effect of cimetidine on proliferation and apoptosis of colon cancer cells LoVo in vitro.Methods:Colon cancer cell line LoVo originated from human colon carcinoma was cultured in vitro.Cell proliferation was measured by MTT colorimetric assay.Cell cycle and apoptotic percentage were detected by flow cytometry (FCM),morphological changes of apoptotic cell were observed by light and electron microscopy,and apoptotic cells were quantitatively determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).In addition,expression of intracellular bcl-2 protein was analysed by flow cytometric indirect immunofluorescent technique.Results:Cimetidine (1?10 -2 mol/L～3?10 -2 mol/L) inhibited LoVo cells growth significantly in a dose-dependent manner.After 48 hours of exposure to cimetidine(1?10 -2 mol/L),the enhanced degree of apoptotic percentage was 32.7%.There were many morphologic features of apoptosis,including cell shrinkage,nuclear condensation,DNA fragmentation and formation of apoptotic bodies;apoptotic cells were also statistically significant than those of control group( P

BACKGROUND:In recent years, delayed protection of drug pretreatment has become a hot spot in the field of pretreatment, and sufentanil is a non-selective opioid receptor agonist that plays a significant myocardial protective role.
OBJECTIVE:To investigate the protective effect of sufentanil combined with umbilical cord blood stem cel transplantation on injured myocardium after myocardial infarction.
METHODS:Myocardial infarction model by ligating the left anterior descending coronary artery (ischemia 30 minutes and reperfusion 180 minutes) was made in 90 healthy adult Wistar rats, and then these rat models were randomly divided into ischemia-reperfusion (IR) group, cel transplantation group, combined treatment group (n=30/group). Rats in the IR group were given intraperitoneal y intravenous injection of 1 mL normal saline 5 minutes before IR;rats in the cel transplantation group were given intraperitoneal y intravenous injection of 1 mL umbilical cord blood stem cel suspension 5 minutes prior to IR;and rats in the combined treatment group were given intraperitoneal y intravenous injection of 10μg/kg sufentanil 10 minutes before IR and 1 mL umbilical cord blood stem cel suspension 5 minutes prior to IR. Two weeks later, related indicators were detected.
RESULTS AND CONCLUSION:Compared with the IR group, myocardial infarct size was reduced significantly in the combined treatment group, fol owed by the cel transplantation group (P<0.05). Lowest levels of serum creatine kinase, lactate dehydrogenase and troponin I were obtained in the combined treatment group fol owed by the cel transplantation group and IR group, while highest level of nitric oxide was observed in the combined treatment group fol owed by the cel transplantation group and IR group (P<0.05). Highest expression of Caspase-3 protein was recorded in the combined treatment group, fol owed by the cel transplantation group and IR group (P<0.05). Significantly increased left ventricular diastolic blood pressure and decreased left ventricular end diastolic pressure were found in the two cel transplantation groups compared with the IR group (P<0.05). Moreover, pathological injury of the myocardial tissues was mitigated in the cel transplantation group and significantly relived in the combined treatment group compared with the IR group. In conclusion, the combined use of sufentanil and umbilical cord blood mesenchymal stem cel transplantation can reduce the degree of myocardial infarction and protect against myocardial injury.

Unit discharges of neurons of the hypothalamic ventromedial nucleus region (HVM) and its surrounding structures including paraventricular nucleus, anterior hypothalamic area,and hypolhalamic dorsomcdial nucleus, were recorded in the ureth-ane-anesthetized and flaxidel-immobilized rats. In HVM,8 of 82 units showed periodical fluctuations in discharge rate, which could be categorized into two types: (1) With alternating active and silent phases 5 (2) with gradually waxing and waning phases. The duration of cach cycle varied from 1 to 5 minutes in different neurons. The periodicity disappeared after intraperitoneal injection of nembutal, while the average discharge rate was raised somewhat. This result suggested that the depressing pha-s of periodical activity of the HVM neuron might probably result from the inhibitory action in certain neuronal circuit, rather than from the intrinsic property of the recorded neurons. The effect of picrotoxin was contradictory. No periodical activity was noted in the 47 recorded neurons outside of the HVM.

alyzing allergen of Alternaria alternata.

asthma is a kind of chronic mucosal inflammation and characterized by eosinophilia,hypertrophy of goblet cell,increment of mucous secretion,reversible airway obstruction and hyperresponsiveness to external and endogenous stimuli.Of them,the recruitment and activation of eosinophil have an important role in the pathogenesis of asthma;the aim of this paper is to review the advancement of recruitment of eosinophil in the pathogenesis of asthma,and make a new method for the treatment of asthma.

Objective To evaluate the validity and predictive value of CAP specific IgE test system.Methods Five allergen-specific IgEs,i.e.,specific IgE to mites,mugwort pollens,hop pollens,cat epithelium and dander,and Alternaria alternate,were tested in selected randomly allergic and non-allergic patients by using CAP system.Results In 957 tests the sensitivity of specific IgE to the 5 allergens was 99.0%,97.9%,91.7%,93.5% and 85.5%,and the specificity was 96.2%,95.8%,93.0%,97.0% and 91.7%.The positive and negative predictive values were greater than 94% and 80% respectively.Conclusion CAP system is of great potential value for diagnosis of allergens.

Objective To find the best methods to make the extraction of alternaria alternate. Methods Alternaria alternate was harvested from air and identified, cultured at 26 ℃ for four weeks. Protein was extracted by different methods and its concentration was determined using the Bradford assay, its bioactivity was tested by RAST inhibition, and residual proteins were analyzed by SDS-PAGE.Results The extraction methods like grinder(a), grinder plus ultrasonic(b), ground with liquid nitrogen(c) and liquid nitrogen plus ultrasonic(d) were applied in the extraction procedure. The concentrations of protein extractions were as follows (g/mL): a was 0.44?0.04;b was 0.75?0.03;c was 0.72?0.03 and d; 1.29?0.05. From SDS-PAGE, we found that the most component in the extraction was that obtained by liquid nitrogen plus ultrasonic. From RAST, 50% inhibition was as follows(g/mL): a was 8.5, b was 9.4, c was 7.0,d was 3.7. Conclusion Ground with liquid nitrogen plus disintegration by ultrasonic was the most effective method of extraction.

The requirement for HCWs grows with the transfer of medical care model and increasing expectation of social population.This paper reviews the Psycho-social education in PUMC for 90 years and creates potential strategies for the Psycho-social education in long-term medical education program in China.

Objective To evaluate the use of tryptase in diagnosing allergic diseases.Methods Serum tryptase was measured by Unicap 100 in 35 patients with different types and states of allergic diseases, and 30 healthy volunteers as controls ;Serum tryptases were compared with IgE level.Results Among the 35 allergic patients, 18 cases were with chronic urticaria(4.74?2.54)?g/L; 10 cases had past histories of anaphylaxis shocks(4.86?2.55)?g/L ;7 cases were in acute phase of anaphylaxis(17.4?7.87)?g/L ;30 healthy volunteers(4.36?1.67)?g/L. A statistically significant increase in tryptase concentration was found in patients with acute phase of anaphylaxis compared with other groups.No correlation was found between tryptase concentration and total IgE level.Conclusions Measurement of Serum tryptase level could be an additional tool for diagnosis of anaphylaxis but not chronic urticaria.

Objective: To prepare the processing solution of realgar by bacteria and to explore the change of pharmacodynamic action and toxicity. Methods: The processing solutions of realgar were prepared using the strain thiobacillus ferroxidans, the realgar powder processed by normal method was selected as control. The effects on RPMI8226 cell proliferation of them were investigated by methyl thiazolyl terazolium (MTT),cell cycle distribution was determined by FCM in RPMI8226 cell. The distribution of arsenic concentration in blood and tissues including heart, liver, spleen, lung, and kidney in the samples were determined by Inductively Coupled Plasma-atomic Emission Spectrometry (ICP-AES). Results: Compared with control group, the solubility rate and the induced apoptotic rate increased, and dosage significantly reduced, while arsenic was hardly found in the tissue in processing group by bacteria. Conclusions: The processing group by bacteria may be enhanced medicinal efficacy and inhibited the toxicity in realgar. It’s intending to provide new approaches for scientific processing realgar.