Objective:To investigate the regulatory effect of cimetidine on proliferation and apoptosis of colon cancer cells LoVo in vitro.Methods:Colon cancer cell line LoVo originated from human colon carcinoma was cultured in vitro.Cell proliferation was measured by MTT colorimetric assay.Cell cycle and apoptotic percentage were detected by flow cytometry (FCM),morphological changes of apoptotic cell were observed by light and electron microscopy,and apoptotic cells were quantitatively determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).In addition,expression of intracellular bcl-2 protein was analysed by flow cytometric indirect immunofluorescent technique.Results:Cimetidine (1?10 -2 mol/L～3?10 -2 mol/L) inhibited LoVo cells growth significantly in a dose-dependent manner.After 48 hours of exposure to cimetidine(1?10 -2 mol/L),the enhanced degree of apoptotic percentage was 32.7%.There were many morphologic features of apoptosis,including cell shrinkage,nuclear condensation,DNA fragmentation and formation of apoptotic bodies;apoptotic cells were also statistically significant than those of control group( P

BACKGROUND:In recent years, delayed protection of drug pretreatment has become a hot spot in the field of pretreatment, and sufentanil is a non-selective opioid receptor agonist that plays a significant myocardial protective role.
OBJECTIVE:To investigate the protective effect of sufentanil combined with umbilical cord blood stem cel transplantation on injured myocardium after myocardial infarction.
METHODS:Myocardial infarction model by ligating the left anterior descending coronary artery (ischemia 30 minutes and reperfusion 180 minutes) was made in 90 healthy adult Wistar rats, and then these rat models were randomly divided into ischemia-reperfusion (IR) group, cel transplantation group, combined treatment group (n=30/group). Rats in the IR group were given intraperitoneal y intravenous injection of 1 mL normal saline 5 minutes before IR;rats in the cel transplantation group were given intraperitoneal y intravenous injection of 1 mL umbilical cord blood stem cel suspension 5 minutes prior to IR;and rats in the combined treatment group were given intraperitoneal y intravenous injection of 10μg/kg sufentanil 10 minutes before IR and 1 mL umbilical cord blood stem cel suspension 5 minutes prior to IR. Two weeks later, related indicators were detected.
RESULTS AND CONCLUSION:Compared with the IR group, myocardial infarct size was reduced significantly in the combined treatment group, fol owed by the cel transplantation group (P<0.05). Lowest levels of serum creatine kinase, lactate dehydrogenase and troponin I were obtained in the combined treatment group fol owed by the cel transplantation group and IR group, while highest level of nitric oxide was observed in the combined treatment group fol owed by the cel transplantation group and IR group (P<0.05). Highest expression of Caspase-3 protein was recorded in the combined treatment group, fol owed by the cel transplantation group and IR group (P<0.05). Significantly increased left ventricular diastolic blood pressure and decreased left ventricular end diastolic pressure were found in the two cel transplantation groups compared with the IR group (P<0.05). Moreover, pathological injury of the myocardial tissues was mitigated in the cel transplantation group and significantly relived in the combined treatment group compared with the IR group. In conclusion, the combined use of sufentanil and umbilical cord blood mesenchymal stem cel transplantation can reduce the degree of myocardial infarction and protect against myocardial injury.

alyzing allergen of Alternaria alternata.

asthma is a kind of chronic mucosal inflammation and characterized by eosinophilia,hypertrophy of goblet cell,increment of mucous secretion,reversible airway obstruction and hyperresponsiveness to external and endogenous stimuli.Of them,the recruitment and activation of eosinophil have an important role in the pathogenesis of asthma;the aim of this paper is to review the advancement of recruitment of eosinophil in the pathogenesis of asthma,and make a new method for the treatment of asthma.

Objective To investigate ICU nurses' perceptions towards incontinence-associated dermatitis.Methods Totally 300 ICU nurses in Suzhou were investigated with a self-designed questionnaire.Results College education was considered to be their main source of knowledge about incontinence-associated dermatitis in 53.2 percent of the nurses.99.8 percent of nurses thought college education was far enough for clinical practice.68.5 percent of nurses thought it was very important to train for knowledge of incontinence-associated dermatitis.Conclusions ICU nurses in Suzhou have less knowledge on incontinence associated dermatitis.It can not meet clinical needs.It is recommended that more incontinence-associated dermatitis knowledge should be added in the school education and continuing education in order to improve nurses' professional competency and to reduce the incidence rate of incontinence-associated dermatitis

BACKGROUND: Fetal microchimerism is a kind of stem cells which comes from fetus in pluripara. The research on fetal microchimerism is a hotspot because it may affect the health condition of pluripara.OBJECTIVE: To introduce the methods of how to detect fetal microchimerism, and the function of fetal microchimerism during disease and tissue repair of the mother.METHODS: Fetal microchimerism was used as a key word, and papers published before 2011-05-20 were retrieved in PubMed. Repetitive papers were eliminated.RESULTS AND CONCLUSION: Totally 655 papers were retrieved, and finally 26 were reviewed. There are several methods used for fetal microchimerism detecting, and fetal microchimerism may pay an important role in the pathomechanism of pluripara autoimmune diseases, cancer and tissue repair.

Objective To evaluate the validity and predictive value of CAP specific IgE test system.Methods Five allergen-specific IgEs,i.e.,specific IgE to mites,mugwort pollens,hop pollens,cat epithelium and dander,and Alternaria alternate,were tested in selected randomly allergic and non-allergic patients by using CAP system.Results In 957 tests the sensitivity of specific IgE to the 5 allergens was 99.0%,97.9%,91.7%,93.5% and 85.5%,and the specificity was 96.2%,95.8%,93.0%,97.0% and 91.7%.The positive and negative predictive values were greater than 94% and 80% respectively.Conclusion CAP system is of great potential value for diagnosis of allergens.

Objective To find the best methods to make the extraction of alternaria alternate. Methods Alternaria alternate was harvested from air and identified, cultured at 26 ℃ for four weeks. Protein was extracted by different methods and its concentration was determined using the Bradford assay, its bioactivity was tested by RAST inhibition, and residual proteins were analyzed by SDS-PAGE.Results The extraction methods like grinder(a), grinder plus ultrasonic(b), ground with liquid nitrogen(c) and liquid nitrogen plus ultrasonic(d) were applied in the extraction procedure. The concentrations of protein extractions were as follows (g/mL): a was 0.44?0.04;b was 0.75?0.03;c was 0.72?0.03 and d; 1.29?0.05. From SDS-PAGE, we found that the most component in the extraction was that obtained by liquid nitrogen plus ultrasonic. From RAST, 50% inhibition was as follows(g/mL): a was 8.5, b was 9.4, c was 7.0,d was 3.7. Conclusion Ground with liquid nitrogen plus disintegration by ultrasonic was the most effective method of extraction.

The requirement for HCWs grows with the transfer of medical care model and increasing expectation of social population.This paper reviews the Psycho-social education in PUMC for 90 years and creates potential strategies for the Psycho-social education in long-term medical education program in China.

Objective To explore the relationship between pituitary tumor transforming gene(PTIG) and Ki67 expression and their significance in multiple adolescent breast fibroadenomas (fibroadenomatosis)and single persons(fibroadenomas) and their clinical value.Methods A total of 53 fibroadenomatosis and 160 fibroadenomas specimens in our hospital were collected.All the samples were fixed with 10％ formalin and then embedded with paraffin.The protein expression of PTTG and Ki67 was detected by immunohistochemical staining.The relation between expression of PTTG and Ki67 in fibroadenomatosis was analyzed.Results The positive rate of PTTG and Ki67 was higher in fibroadenomatosis than in fibroadenomas (P ＜ 0.005).Conclusions High expression of PTTG and Ki67 in fibroadenomatosis leads to the proliferation of tumor cell and upregulates tumor angiogenesis.High expression of PTTG and Ki67 may play important roles in tumorigenesis and pathological process of fibroadenomatosis.