Objective To study the effects of oxidative stress on apoptosis of bovine retinal capillary pericytes(BRPs) under high glucose condition.Methods BRPs were cultmred for 6 days respectively in control group(5.5 mmol/L)and in high glucose conditions (15,25,35 mmol/L).The sub-cellular structure was observed under electron microscope.Contents of malondialdehyde ( MDA ) and superoxide dismutase ( SOD ) activities were detected respectively.Results The BRPs showed typical apoptotic morphograpy in the high glucose conditions with the apoptosis rates of(28.35 ±0.84)％,(40.43 ±0.93)％ and(50.16 ±0.60)％ in 15 mmol/L,25 mmol/L and 35 mmol/L glucose condition,much higher than that of control group (4.41 ± 0.66) ％ ( F =21.97,P ＜ 0.01 ).The MDA content/SOD activity of BRPs in high glucose groups were higher than those in the control group(P ＜ 0.01 ).The MDA content/ SOD activity was positively correlated with the apoptosis ratc of BRPs( r =0.893,P ＜ 0.01 ).Conclusion Oxidative stress may participated in the apoptosis of bovine retinal capillary pericytes under high glucose condition.

Objective Compared with povidone iodine solution to clean,glutaraldehyde immersion,highpressure steam sterilization of three disinfection methods for dental handpiece sterilization effect.Methods Select the 20 dental clinical used over phone,were randomly divided into A group,B group,C group,D group 5,A group for the control group,only cleaning method did not use any disinfection,B group was 1% Polyvinylpyrrolidone iodine solution,wipe,C group were soaked in 2% glutaraldehyde soluton,D group were treated with high-pressure steam sterilization,after each phone were inoculated with bacteria sample dish,the more monitoring of four groups of bacterial culture.Results A group of bacterial culture for the intensive growth of bacteria,B group had bacterial growth for the(+ ～+ + +),bacterial growth;C group had bacterial culturegrowth was(+ ～ + +),bacterial growth;D group of bacterial culture Growth of(-),no bacterial growth.Conclusion High-pressure steam sterilization was the disinfection of dental handpieces most effective way.

Objective To study the lung protection of ambroxol in general thoracic surgery perioperation for the elderly.Methods Totally 130 hospitalized patients aged (65.1±4.3) years in our hospital during general thoracic surgery were randomly divided into two groups:ambroxol intervention(2 mg/kg,intravenous drip,once/d) and control groups (n=65 for each).The incidenceof pulmonary complications,changes of blood gas analysis before and after surgery and time of hospitalization were compared between the groups.Results The incidence of pulmonary complications was significantly lower in ambroxol intervention group than in control group (9.23％ vs.24.62％,P＜0.05).Blood gas analysis results before and after surgery were improved in ambroxol intervention group[PaO2:(61.32±8.65) mm Hg,(95.07±13.62)mm Hg; PaCO2:(44.15±5.52)mm Hg,(37.28±4.06)mm Hg] as compared with control group[PaO2:(62.11 ± 8.83) mm Hg,(80.37±10.46)mm Hg; PaCO2:(44.39±5.17) mm Hg,(40.73±4.51)mm Hg] (t=3.61,3.75,both P＜0.05).The time of hospitalization was shorter in ambroxol intervention group than in control group [(12.14±0.29) d vs.(15.85±0.62)d,t=3.41,P=0.036].Conclusions Perioperative use of ambroxol in general thoracic surgery in the elderly is worthy of clinical application because of reduction of pulmonary complications and shortness of hospital stay.

Objective To discuss the correlation of the form stigmata of maximus thenar dermatoglyphic patterns of palm and allergic dermatitis. Methods The allergic dermatitis patients were rardanly selected as observation group and normal person or non-suffering allergic dermatitis as control group. The maximus thenar dermatoglyphic patterns of palm were classified into three grades on the basis of its tend towards, distance, depth and character of cleavage lines, and the degree of moistening, dryness, softness, smoothness of surface skin, etc. Results There are 82 examples in observation group, grade Ⅰ is 11 examples, degree Ⅰ of grade Ⅱ is 17 examples, degree Ⅱ of grade Ⅱ is 31 examples, grade Ⅲ is 23 examples. There are 86 examples in control group, grade Ⅰ is 71 examples, degree Ⅰ of grade Ⅱ is 5 examples, degree Ⅱ of grade Ⅱ is 6 examples, grade Ⅲ is 4 examples. There is significant difference between the observation group and control group (P

Objective To investigate the impact of different irrigation time on oxygenation in-dex and acid-base balance during percutaneous nephrolithotomy(PCNL).Methods Forty patients un-dergoing selective PCNL were included in this study,were divided into two groups:group S,irrigation time<120 min(n=22);group L,irrigation time≥120 min(n=18).PaO2/FiO2 ,pH and BE were determined before and after irrigation,CVP were recorded before irrigation and every 20 min during irrigation. Results The CVP maximum value were higher in group L than that in group S during irrigation (P <0.05).The PaO2/FiO2 were lower after irrigation than that before irrigation in group L(P<0.05).The pH and BE were lower in group L than that in group S after irrigation(P<0.05).Conclusion Irrigation fluid absorption is observed during PCNL.That can provoke oxygenation function decline and metabolic ac-idosis with irrigation time extend,the operation time properly shortened.

Objective To observe the effects of dexmedetomidine on inflammatory responses and insulin resistance during cardiac operations with cardiopulmonary bypass(CPB).Methods Fifty patients scheduled for elective cardiac valve replacement with CPB were equally and randomly divided into two groups using a random number table:observation group and control group(n =25).A loading dose of dexmedetomidine 1.0 μg/kg was injected intravenously over 1 5 min after induction,followed by continuous infusion at 0.4 μg·kg-1 ·h-1 until the end of CPB in doservation group.While the e-qual volume of normal saline was given in control group.After induction(T1 ),at 30 min after the be-ginning of CPB(T2 ),at the end of CPB(T3 ),and at 2 h after the end of CPB(T4 ),the jugular venous blood samples were taken for determination of serum concentrations of TNF-a,IL-6,insulin and blood glucose.The insulin sensitivity index (ISI ) = 1/(glucose × insulin )was obtained. Results Compared with control group,the serum TNF-a and IL-6 concentrations at T2-T4 were sig-nificantly decreased in observation group(P <0.05),the serum insulin and blood glucose concentra-tions at T2 ,T3 were significantly decreased in observation group(P <0.05 ),the ISI at T2 ,T3 were significantly increased in observation group(P <0.05).Conclusion Dexmedetomidine can reduce in-flammatory responses,thus reducing insulin resistance and blood glucose during cardiac operations with CPB.

Objective:To investigate the effect and application feasibility of low tube voltage, low concentration of contrast agent volume to CTA in the diagnosis of coronary artery stenosis and its effect.Methods: Seventy two cases of suspected patients with coronary atherosclerosis selected as the object Using random number table method, all patients were divided into group A and group B 36 cases in each group, two groups of patients underwent 320 slice CTA examination, in group A the tube voltage is 120kV, the contrast agent was 350mgI/mL iodixanol group, B tube voltage is set to 100kV, the contrast agent was 270mgI/mL iodixanol. According to the examination results of group A, group B and catheter coronary angiography (CCA), evaluated group A, group B of acquiring the image quality and radiation dose of patients, and analyzed the sensitivity of A, B and CCA examination of the extent of coronary artery stenosis degree and deliberately.Results: The quartering method showed no significant difference between the group A and the group B of image quality. There was significant difference between the two groups of patients with the image noise and enhance the effect (t=-4.736, 11.927;P<0.05); Group B of coronary artery stenoses (more than 50%) and severe stenosis (more than 75%) sensitivity, specially degree and coincidence rate compared with the results of CCA showed no significant difference. Kappa consistency analysis showed that the two consistency is good(kappa=1.320,P<0.001).Conclusion: In the premise of ensuring the image quality with low tube voltage, low concentration of contrast agent volume coronary CTA examination had safety and reliability of inspection.

Radiation therapy is one of the main means of treatment for non-small cell lung cancer (NSCLC). With the rapid development of modern technology, three dimensional conformal radiotherapy, intensity modulated radiotherapy, stereotactic radiotherapy play a very important role in this field. The use of the state-of-art radiation modality can definitely improves the accuracy of the treatment, which results in improving the local control rate of tumor and reducing the adverse reaction of normal tissue. Advances in modern imaging device and its clinical application is the premise of precise radiotherapy. Positron emission tomography and computed tomography (PET-CT) can effectively provide anatomical and functional information; it plays a more and more important role in the diagnosis of NSCLC before radiotherapy, the process of precise target delineation and also the evaluation of therapeutic efficacy after radiotherapy.

Objective:To investigate the 256 slice spiral CT pulmonary angiography (CTPA) application value by using multiple stage double mixed injection velocity tracking method in the diagnosis of pulmonary embolism.Methods: Fifty patients with pulmonary embolism from 2014 January to 2015 January were treated in our hospital patients as the research object. Using a random number table method, all patients were randomly divided into observation group and control group, 25 cases each group of patients, two groups of patients in our hospital by imaging with 256 slice spiral CT pulmonary artery (CTPA), the control group were treated by routine pre injection time density curve method, was used in the observation group than in mixed injection agent tracking method by two physicians, CT, using the double-blind assessment of two groups of patients with image quality and quantification of pulmonary artery trunk.Results: in the control group, 25 patients were satisfied with the time - density curve, the results of 5 conventional image quality scores showed no significant difference between the two imaging methods, was not statistically significant; two groups of patients with pulmonary artery trunk, branch clear imaging score and endovascular CT value had no significant difference(t=1.892,t=1.952,t=1.832;P>0.05), but the observation group patients with CTPA were examined by contrast agent dose was significantly lower than the control group, the patient''s heart rate, blood pressure was significantly lower than the control group(t=2.315,t=1.958,t=2.982;P<0.05), with statistical significance. Conclusion: CTPA with multi period mixed double flow injection method can guarantee the tracking based on image quality and diagnostic value can effectively reduce the contrast agent dosage and application effects of cardiac function, and the examination is relatively simple, safe, has clinical value of application and popularization.

Objective To discuss the opportunity of transurethral resection of prostate (TURP) after transrectal prostate biopsy.Methods We analyzed 60 cases of benign prostatic hyperplasia(BPH) who underwent TURP after transrectal prostate biopsy from September 2013 to September 2015.All the patients were divided into either group A or group B in chronological order, with 30 cases in each group.There were no significant differences in age, prostate specific antigen (PSA), prostate volume, hemoglobin level, and international prostate symptom score (IPSS) between the two groups.The group A and group B were treated by TURP at 1 week and 4 weeks after transrectal prostate biopsy, respectively.The parameters including operation time, excised prostate weight, intraoperative total blood loss, bladder irrigation time, and IPSS at 3 months after operation were recorded.Results The operation time, intraoperative total blood loss, bladder irrigation time, and IPSS in the group B were significantly lower than those in the group A [(58.3±6.0) min vs.(62.0±3.3) min, t=2.952, P=0.005;(154.1±15.8) ml vs.(167.4±29.5) ml, t=2.181, P=0.035;(19.2±0.8) h vs.(20.6±2.3) h, t=3.034, P=0.004;(18.3±2.5) points vs.(20.3±2.0) points, t=3.419, P=0.001].The excised prostate weight in the group B was significantly higher than that in the group A [(37.1±4.0) g vs.(33.3±7.8) g, t=-2.341, P=0.024].Conclusions TURP performed at 4 weeks after transrectal prostate biopsy can significantly increase the excised prostate weight, reduce intraoperative total blood loss volume, shorten the operation time and postoperative bladder irrigation time, and improve urinary symptoms.In brief, we recommend that TURP be executed at 4 weeks after transrectal prostate biopsy.