【Objective】 To study the effects of docosahexaenoic ac id (DHA) and arachidonic acid (AA) intake on the visual and cognitive function o f preterm infants. 【Methods】 32 preterm infants (gestational age<37 weeks) wer e divided into three groups which were fed with different formula respectively: group A, breast milk(n=11); group B, a conventional formula lacking DHA and AA (n=10); group C, DHA and AA enriched formula (n=11). Group C was stop ped to fed enriched formula when infant's weight reached (2.50±0.10) kg. Cognitiv e function was evaluated by NBNA test when the corrected age of each preterm inf ant was 42 week±7 day, ERG of both eyes was tested at three-months old. The ex periment lasted for three months. 【Results】 Cognitive and visual function of g roup C were similar to group A, however, some indexes of group B were significan tly lower than group A and C (P<0.05). 【Conclusion】 Adding DHA and AA to f ormulas similar to breast milk in amounts, can improve cognitive and visual func tion of preterm infants, and help these infants to achieve similar cognitive and visual function to those breast-milk-fed preterm infants.

Objective To study the effect of laparoscopic surgery on the quality of life in patients with middle and low rectal cancer.Methods 108 cases with middle and low rectal cancer in accordance with the number table,were randomly divided into two groups.54 patients in the observation group were operated through laparoscopic surgery and 54 patients in the control group received traditional open surgery.The patients were followed up for 6 months,the patients' quality of life were assesed.Results The operative time,blood loss and length of hospital stay in the observation group were less than the control group (P ＜ 0.01).The lymph node dissection between the two groups had no significant difference(P ＞0.05).47 patients in the observation group were rectal sphincter preservation,the rate was 87.0％,37 patients in the control group with rectal sphincter preservation,the rate was 68.5 ％,the difference between the two groups was significant (x2 =4.92,P ＜ 0.05).The patients'quality of life after surgery in two groups was increased than before surgery,and the observation group increased more significantly than the control group(P ＜ 0.05).Conclusion Laparoscopic surgery can significantly improve the quality of life in patients with middle and low rectal cancer.

Objective To explore the influence of diabetes on the protective effect of ischemic preconditioning(IPC) on ischemic-reperfused(IR) myocardium of rat.Methods Sixty male SD rats(30 with diabetes and 30 without diabetes) were divided into six groups(10 each): non-diabetic rats control group(group A),non-diabetic rats IR group(group B),non-diabetic rats IPC group(group C),diabetic rats control group(group D),diabetic rats IR group(group E) and diabetic rats IPC group(group F).The isolated heart perfusion Langendorff models were established after rats were sacrificed.Isolated hearts of the two control groups were undergone a 90min perfusion without any other intervention;those of the two IR groups were undergone a 30min equilibration period,a 30min ischemia and a 30min reperfusion;those of the two IPC groups were undergone a 10min equilibration,then elicited by two cycles of 5min ischemia interspersed with 5min reperfusion prior to 30min ischemia and a 30min reperfusion.The recovery rates of the left ventricular function,such as cardiac output(CO) and left ventricular developed pressure(LVDP),and the maximum upstroke and decay velocities of left ventricular pressure(?dp/dtmax) were recorded.The activity of creatine kinase(CK) in coronary outflow,contents of malonyldialdehyde(MDA) and superoxide dismutase(SOD) in myocardium were detected,and myocardial water ratio were assessed.Results In non-diabetic rats IPC group,the activity of CK,content of MDA and water ratio of myocardium declined significantly,while the content of SOD increased and the recovery rates of CO,LVDP and ?dp/dtmax rose significantly compared with that in non-diabetic rats IR group(P0.05).Conclusion Diabetes may inhibit the protective effect of ischemic preconditioning on ischemic reperfused rat heart.

Objective: To examine the correlation of symptomatic feature and exploratory eye movements in schizophrenia. Methods: The eye movements of 60 patients with schizophrenia and 30 healthy control subjects were measured by the eye-mark recorder (model IV, Japan) and symptom profiles of patients were assessed using the Positive and Negative Syndrome Scale (PANSS) . Pearson correlation analysis was performed to examine the relationship between five-factor scores of PANSS, positive symptoms, negative symptoms and exploratory eye movements respectively. Results: The differences of all indices of eye movements between schizophrenic groups and normal control group had statistical significance ( P

The flushing pump which is applied to clean operative wound has no temperature controlling function up to now, and doctors have to prepare the flushing fluid that has previously been warmed. The flushing pump system with medical constant temperature designed in our laboratory can absorb flushing fluid at the room temperature, and then eject flushing fluid with the temperature in accordance with the requirements of operations at a controlled constant flow rate. The system combines flow rate control with temperature control functions. The flushing pump system includes flushing part, temperature controlling part, key inputting part, liquid crystal displaying part and exceptional situation monitoring part. The present paper introduces the design method and principle of each part of the system at first, and then gives the debug method of all the system parameters. Finally the paper discusses the performance of the system according to the result of the experiment.
Equipment Design ; Equipment and Supplies ; Temperature

Objective:To investigate the relationship between the pathogeny and expression of CD28 B7 costimulatory molecules on peripheral blood cells in myasthenia gravis.Methods:Expression of CD28?CTLA4 and their ligants B7 1?B7 2 on CD4 + and CD8 + T lymphocytes in 18 patients with MG and 16 healthy controls were examined by flow cytometry.Results:In MG group,the expression of CD28?CTLA4?B7 1?B7 2 molecules were increased.The increased CD28 + cells were mainly of CD4 +T lymphocytes subset.The increased CTLA4 + cells were mainly of CD8 + T lymphocyte subset;There was no significant difference in the expressions of B7.1 and B7.2 molecules on CD4 + or CD8 + T lymphocytes between two groups.Conclusion:The pathogeny of myasthenia gravia is associated with the increased expression of CD28 B7 costimulatory molecules.Examining of CD28 B7 costimulatory molecules on peripheral blood cells may reflect the immune state of MG patients,and is helpful to the diagnosis and treatment.

Background and purpose:The signal transducers factor and activator in transcription 3(STAT3) is a recently studied gene from a variety of solid tumors such as breast, stomach, and ovarian cancer, in which the increase of abnormal expression and activity are accompanied.The aim of this study was to investigate the effects of eukaryotic vectors that express short hairpin RNA(shRNA) in signal transducers and activators of STAT3 on chemosensitivity of human ovarian cancer SKOV3 cells.Methods:Vectors containing shRNA targeting STAT3(pSTAT3-siRNA) were constructed and transfected into SKOV3 cells.These vectors were then divided into 3 groups:SKOV3, SKOV3NS, and SKOV3siRNA.The mRNA and protein expressions of STAT3 were determined by RT-PCR and Western blot, respectively.The growth inhibition and apoptosis rates of the different group cells under chemotherapeutic agents such as cisplatin(20 ?mol/L) were measured by MTT assay and flow cytometry(FCM).Results:MTT assay growth inhibition rates in the tumor cells of the SKOV3 group, SKOV3NS group and SKOV3siRNA group had inhibition rates of 0.46?0.13, 0.44?0.11 and 0.71?0.12.Compared with the SKOV3, SKOV3NS and SKOV3siRNA group, there was a marked increase of SKOV3siRNA group in inhibition rate of cells.The differences were also statistically significant(P0.05).The SKOV3 group, SKOV3NS group and SKOV3siRNA group's cell apoptosis rates were 18?4, 18?3 and 35?4, respectively.However, the SKOV3 group, SKOV3NS group, and SKOV3siRNA group cell apoptosis were significantly increased and the differences were statistically significant(P0.05).The results for the SKOV3 group, SKOV3NS group and SKOV3siRNA in cell STAT3 mRNA were 0.50?0.08, 0.48?0.07 and 0.31?0.09.With the SKOV3 group, SKOV3NS group, SKOV3siRNA group of cells in STAT3 mRNA, its expression in the lungs were significantly lower and the differences were statistically significant(P0.05).SKOV3 group, SKOV3NS group and SKOV3siRNA group of cells checked the results of STAT3 protein were 0.54?0.09, 0.56?0.08 and 0.32?0.09, respectively.The SKOV3 group, SKOV3NS group, and SKOV3siRNA group in STAT3 protein expression was significantly lower and the differences were statistically significant(P0.05).Conclusion:The STAT3 specific shRNA expression vector could effectively suppress the expression level of STAT3 gene in SKOV3 cells as well as enhance their sensitivity to cisplatin.

Objective To determine whether it is necessary to execute pre-labor pubic shaving.Methods Women(336 cases)who received pubic shaving through vaginal delivery from 2003 to 2006 were set as the control group.Women(103 cases)who were waiting for delivery currently in our hospital were set as the test group.The test group only received perineal cleaning without pubic shaving.If episiotomy was needed during the operation,the perineal hair Was cut by scissors.The perineum infectious rate and postpartal infection Was compared in the two groups.We interviewed the patients attitude for pubic shaving and counted the numing time used for pubic shaving.Results The perineal infection in the test group was 5.8%,which was no statistically different from that of the control group(2.4%),U=0.33,P＞0.05.Totally 95% women in the test group support this method demonstrated humanization.In the telephone folow-up visit for patients in the control group they comphined itch and discomfort during the regeneration of pubes.The average numing time for pubic shaving Was(4.6±0.6)minutes.Conclusions No pubic shaving for pre-labor skin preparation was feasible.If operation was disturbed by pubes we could use scissors to cut it short.This method could reduce the skin infection and propagation of blood-derived infectious disease.It increased the comfort of parturient,saved time and proved to be a nursing method of humanization.

Objective To compare effectiveness,performance,onset time and complications between ultrasound-guided cervical transverse process block and interscalene brachial plexus block in patients undergoing proximal humeral surgeries.Methods Sixty patients,27 males and 33 females, aged 18-70 years,scheduled for proximal humeral surgeries were randomly divided into two groups. They were given either cervical transverse process block (group T,n =30)or ultrasound-guided in-terscalene brachial plexus block (group I,n =30).All patients received a total of 8 ml of 0.5% ropiv-acaine.The performance, anesthetic onset time, the side effects and block success rate were evaluated.Results Block procedure was quicker in group T than in group I [(8.73 ±3.1 7)min vs. (14.40±8.21)min,P <0.01].The severity of diaphragmatic paralysis in group T was significantly lower than in group I (P <0.01).The ultrasound-guided cervical transverse process block was more effective than the interscalene brachial plexus block in patients undergoing proximal humeral surgeries (100% vs.80%,P <0.05).Conclusion The ultrasound-guided cervical transverse process block has a higher success rate and fewer incidence of diaphragmatic paralysis than the interscalene brachial plexus block in patients undergoing proximal humeral surgeries.

Objective To observe the effects of chronic arsenic exposure on mRNA expression of estrogen receptor-binding fragment-associated gene 9 (Ebag9) and estrogen-responsive finger protein (efp) in uterus and ovary of female rats.Methods Fifty female Wistar rats were randomly divided into five groups according to arsenic (As2O3) concentrations given through drinking-water:0.00 (control),0.05,0.10,0.20,0.40 mg/L arsenic exposure groups and real-time RCR (RT-PCR) was used to detect the mRNA expression of Ebag9 and efp in uterus and ovary tissue at the 31 weeks of experiment.Results The mRNA expression levels of Ebag9 and efp of the 0.00,0.05,0.10,0.20,0.40 mg/L arsenic exposure groups were respectively as follows:0.761 ± 0.178,0.521 ± 0.130,0.544 ± 0.035,0.525 ± 0.198,0.498 ± 0.240 and 0.795 ± 0.171,0.874 ± 0.077,0.797 ± 0.066,0.796 ± 0.040,0.832 ± 0.096.Compared with control group,a decreased tendency was observed in Ebag9 mRNA level(with P value 0.055 in 0.40 mg/L arsenic exposure group) and increased tendency in efp mRNA level in experimental groups (all P ＞ 0.05).The mRNA expression levels of Ebag9 and efp in ovary of the five groups were by turns:0.702 ± 0.484,0.719 ± 0.336,0.693 ± 0.095,0.706 ± 0.055,0.728 ± 0.073 and 0.924 ± 0.061,1.009 ± 0.034,0.930 ± 0.085,0.929 ± 0.068,1.012 ± 0.101.Compared with control group,the expression level of Ebag9 mRNA showed a increased tendency in 0.05,0.20,0.40 mg/L arsenic exposure groups(all P ＞ 0.05).The efp mRNA level increased in experimental groups,with significant difference in 0.05,0.40 mg/L groups (all P ＜ 0.05).Conclusions The expression of efp mRNA has changed in ovary of female rats exposed to chronic arsenic.Arsenic may act as an environmental endocrine disruptor to exert its effect.