1.Drug-resistance of Klebsiella pneumoniae Causing Nosocomial Infections
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To investigate drug-resistance status of Klebsiella pneumoniae(KPN) in nosocomial infections and provide the reference for rational application of antibiotics.METHODS According to Nationwide Rules of Clincal Laboratory Manipulation,the strains were identified and drug-sensitivity test was performed by K-B methods.The extended-spectrum-?-lactamases(ESBLs) producing strains were detected by double-disc synergy test and disc confirmed test.RESULTS Totally 356 strains of KPN were mainly isolated from respiratory department,gerontology department,neurosurgery department and chest surgery department.The specimens were mostly sputum,pharynx swab,urine and secretion.The detection rate of ESBLs-producing KPN was 36.0%.The drug-resistance rate to common antibiotics in ESBLs producers was generally higher than that in ESBLs nonproducers.No KPN was resistant to imipenem and meropenem.CONCLUSIONS Drug-resistance status of K.pneumoniae in nosocomial infections is very serious.We should strengthen monitoring and controlling of it.
2.Distribution and Drug-resistance of Pseudomonas aeruginosa in Nosocomial Infections
Liansheng HUANG ; Hongyu DENG ; Hongkun TAO
Chinese Journal of Nosocomiology 2006;0(07):-
OBJECTIVE To investigate the distribution and drug-resistance of Pseudomonas aeruginosa(PAE) in nosocomial infections to provide the scientific evidence for the clinical use of the antibiotics.METHODS PAE was cultured and identified according to the National Rules of Procedures Clinical Laboratory.Drug resistance was analyzed by K-B methods.RESULTS A total of 318 strains of PAE were mainly isolated from intensive care unit(ICU),departments of cerebral surgery and chest surgery.Drug-resistance of PAE to 14 kinds of antibiotics showed that SXT(91.2%),cefotaxime(89.9%),aztreonam(70.1%),gentamicin and ciprofloxacin(69.8%),respectively.And the resistance to piperacillin,levofloxacin and cefepime was 50.0%.CONCLUSIONS Drug-resistance status of P.aeruginosa is very serious.Hospital should strengthen monitoring and controlling of it.
3.Expression and clinical significance of EGFR and Her-2 in gastric cancer
Hongyu ZHANG ; Hongtao CHEN ; Wanfang DENG
Cancer Research and Clinic 2010;22(11):730-732,735
Objective To analyze the relationship between the survival and expressions of EGFR and Her-2, and explore the prognostic significance of EGFR and Her-2 in gastric cancer patient by detecting the expressions of EGFR and Her-2 in gastric cancer specimens. Methods EGFR and Her-2 were detected by immunohistochemistry. The relation between their expressions with their clinical characteristics and prognosis were analyzed. Results The over-expression rates of EGFR and Her-2 are 27.4 % and 32.1% respectively in 84 cases. The over-expression rates of EGFR and Her-2 in patients with Ⅲ + Ⅳ stages were significantly higher than those with Ⅰ + Ⅱ stages(respectively, P =0.014 and P =0.005). The positive rate in patients with T3+T4 stages were significantly higher than those with T1+T2 stages (P=0.033); and the positive rate in patients with positive lymph node was higher significantly than that with negative (P =0.033). The OS and PFS in over-expression patients were significantly longer than low-expression. Conclusion The overexpression of EGFR and Her-2 could be used to evaluate the degree of differentiation and prognosis of patients with gastric cancer.
4.A comparative study of cone-beam computed tomography and periapical radiography in the diagnosis of external apical root resorption
Yanling DONG ; Jun CHEN ; Feng DENG ; Leilei ZHENG ; Hongyu REN
Chongqing Medicine 2014;(19):2404-2406
Objective To compare the accuracy of cone-beam computed tomography (CBCT ) and periapical radiography in the diagnosis of simulated external apical root resorption (EARR) .Methods The study sample comprised 160 single-rooted premolars for simulating 4 degrees of EARR :no(intact teeth) ,mild(cavity of 1 .0 mm in diameter and depth in root surface) ,moderate(1 .0 mm root shortening) ,and severe(3 .0 mm root shortening) .Two sets of radiographic images were acquired with CBCT and periapi-cal radiography .The severity for all resorption lesions were evaluated blindly by one calibrated examiner .The percentages of correct classification of each degree of EARR were compared between the two imaging techniques using McNemar test .Results With CBCT method ,the percentages of correct classification of no ,mild ,moderate ,severe and all EARR were 95 .0% ,97 .5% ,42 .5% , 87 .5% and 80 .6% ,respectively ;with periapical radiography method ,the percentages were 85 .0% ,42 .5% ,70 .0% ,92 .5% and 72 .5% ,respectively .Significant differences were found between the two imaging techniques for evaluating mild ,moderate ,and all-EARR(P<0 .05) .Conclusion CBCT imaging is more reliable than periapical radiography for detecting EARR ,whereas it is not sensitive to moderate root shortening .In orthodontic practices ,CBCT could be applied to the early diagnosis of EARR ,in order to help make the decision on continuation and modification of orthodontic treatment .
5.Effects of antisense oligonucleotide on endogenous human bFGF, bFGF mRNA and FGFR1 in tumor cell SWO-38
Junjian XIANG ; Yanfang QIN ; Ning DENG ; Hong WANG ; Hongyu YANG
Chinese Journal of Immunology 1985;0(05):-
Objective:To clarify the contribution of endogenous bFGF, bFGF mRNA and FGFR1 to the abnormal growth and phenotypic transformation of neoplastic tumors cells.Methods:The antisense oligonucleotide primers was used to evaluate the influence of endogenous bFGF on growth of human glioma malignant cell lines SWO-38 in vitro. MTT was used to examine the variety of cells growth treated with bFGF antisense oligonucleotide primers. The methods of ELISA, in situ hybridization, immuno-hischemistry and image analysis were used to detect the expression level of bFGF, bFGF mRNA and FGFR1. The colony formation of cells in soft agar was used to assess the cloning efficiency of the cells after exposed to bFGF antisense oligo-nucleotide primers.Results:The cells multiplication, expression of bFGF mRNA and FGFR1 was inhibited by bFGF antisense oligonucleotide primers,and the cells multiplication was dose-dependent. Treated with antisense oligo-nucleotide primers, the expression of FGFR1 and secretion of bFGF were distinctly reduced, and the inhibition efficiency of cells multiplication of WSO-38 was 48% and the inhibition efficiency of colonies of SWO-38 in soft agar was 35%. The inhibition of cells multiplication can be reversed completely by external bFGF, and the reverse efficiency was 8%.Conclusion:The synthesis of bFGF mRNA and expression of bFGF can be specifically inhibited by antisense oligonucleotide, but the inhibition can be cleared up with the addition of external bFGF. The study suggested that the bFGFantisense oligonucleotide could have good effect in inhibiting of tumor under special condition.
6.Involvement of bFGF in the lung response to silica in a mouse model
Junjian XIANG ; Jiayin SUN ; Ning DENG ; Hongyu YANG
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To investigate the relationship between basic fibroblast growth factor (bFGF) and the development of silicosis in mice. METHODS: MTT test was utilized to examine the effects of bFGF-neutralizing antibody and the bronchoalveolar lavage fluid (BALF) of the mice exposed to silica on lung fibroblast cell growth. RESULTS: BALF from mice treated intrabronchially with silica promoted the growth of lung fibroblasts and anti-bFGF antibody inhibited the effect of BALF dramatically. CONCLUSION: These results indicates that bFGF secretion increases in lung in a mice silicosis model and participates in the development of silicosis.
8.Exploration on postgraduate education and talent training for Master of Applied Statistics in medical colleges
Hongyu ZHOU ; Bin PENG ; Xiaoni ZHONG ; Dan DENG
Chinese Journal of Medical Education Research 2021;20(4):413-417
In medical field, the demand of high-level applied statistical talents is becoming more and more urgent, and the number of medical colleges to train graduate students of Master of Applied Statistics (MAS) is increasing gradually. Taking Chongqing Medical University as an example, this article introduces the objective, orientation and training mode of MAS education, analyzes the main characteristics of MAS professional education and talent training, discusses the problems mainly on training system and teaching base, combined with the interview results of MAS graduate students studying in CMU, and puts forward target suggestions including expanding the enrollment scale, optimizing the "two-tutorial system", advancing the training system and developing new teaching bases, to provide reference and experience for the teaching and training of MAS graduate students in medical colleges.
9.Analysis on bacterial culture of bronchoalveolar lavage fluid from 1 693 children with refractory pneumonia
Baoxing HUANG ; Jikui DENG ; Hongmei WANG ; Yan ZHANG ; Ruizhen ZHAO ; Hongyu CHEN ; Heping WANG ; Dongli MA
Chinese Journal of Infection Control 2015;(6):379-382
Objective To evaluate pathogens and antimicrobial resistance of pathogens causing refractory pneumonia in children.Methods Children with refractory pneumonia who admitted to a hospital between May 2008 and December 2014 were performed bronchoscopy,and bronchoalveolar lavage fluid (BALF)were performed bacterial culture and antimicrobial resistance testing.Results 1 693 patients were recruited in the study,273 bacterial isolates were isolated from BALF speci-mens of 226 children,gram-positive bacteria accounted for 38.10% (104/273 ),the main gram-positive bacteria were Streptococcus pneumoniae (n=71)and Staphylococcus aureus (n=23);gram-negative bacteria accounted for 58.24%(159/273),including 44 isolates of Haemophilus parainfluenzae ,28 Klebsiella pneumoniae ,19 Escherichia coli ,and 17 Pseud-omonas aeruginosa ;10 isolates of fungi were also detected,8 of which were Candida albicans .The sensitivity of Streptococ-cus pneumoniae to quinolones,ceftriaxone and cefotaxime were high.Methicillin-resistant Staphylococcus aureus (MRSA) positive rate was 26.32%.ESBLs-producing rate of Haemophilus parainfluenzae and Klebsiella pneumoniae was 32.72% and 62.96% respectively.Conclusion The major pathogens causing refractory pneumonia were Streptococcus pneumoniae and Haemophilus parainfluenzae ,empirical treatment should be conducted accordingly,antimicrobial resist-ance should be considered if therapeutic effect is poor,and targeted therapy should be performed according to cultured re-sults and antimicrobial susceptibility testing result.
10.The effect of NK-1 tachykinin receptor antagonist on hypoxia induced hepatic function injury and hepatocellular apoptosis in rats.
Journal of Biomedical Engineering 2011;28(5):992-996
To investigate the effect and mechanism of NK-1 Tachykinin receptor (NK-IR) antagonist on hypoxia induced hepatic injury, we established the hypoxic rat model. 30 male SD rats (weighing 240-300g) were randomly divided into 3 groups, control group, and experimental groups including the hypoxia group and the NK-1R antagonist group. The rats of experimental groups underwent hypoxia, among them the NK-1R antagonist group were those with interference of NK-1R antagonist by intraperitoneal injection. Hepatic injury was evaluated by pathological staining, hepatic function detection and hepatocellular apoptosis determination. Results showed hypoxia-induced hepatic injury in rats was established successfully. Edema,ballooning degeneration and spotty necrosis were found in livers in the experimental groups, among which the pathological injury in the hypoxia group was worse than that in the NK-1R antagonist group. Moreover,GGT and the rate of hepatocellular apoptosis in the NK-1R antagonist group were obviously lower than that in the hypoxia group (P<0.05). But no significant difference were found in ALT,AST and ALP between groups (P>0.05). These data indicate that Substance P possibly participate in the process of hypoxia-induced hepatic injury, and NK-1R antagonist could reduce hypoxia-induced hepatic injury.
Animals
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Apoptosis
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drug effects
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Hepatocytes
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pathology
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Hypoxia
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pathology
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physiopathology
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Liver
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physiopathology
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Liver Function Tests
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Male
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Neurokinin-1 Receptor Antagonists
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Random Allocation
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Rats
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Rats, Sprague-Dawley