Objective To evaluate the differences between fresh and cryopreserved human umbilical cord blood as well as the stem cell homing.Methods Fresh or cryopreserved human umbilical cord blood cells were injected into lethally irradiated BALB/C nu+ mice.The mice survival rates,the successful transplantation evidence and the migration phenomenon were observed.Results The survival rates of the fresh group and the cryopreserved group were much higher than the control group,but there was no difference between the former two groups.Evidence of human hematopoiesis were observed by means of PCR or FACS.However,the pattern of migration was different berween fresh and cryopreserved cord blood stem cells. Conclusion Human cord blood contains high hematopoietic potential stem cells to cross xenogenetic barriers and successfully engraft in mice.Cryopreservation can keep the hematopoietic potential of cord blood,but may influence the adhesion molecular of the stem cells and thus influence their homing behavior.

Fluid-attenuated inversion recovery vascular hyperintensity (FVH) is a common magnetic resonance imaging findings in acute ischemic stroke due to severe stenosis or occlusion of large cerebral arteries.This article reviews the applications and related research of FVH in patients with acute ischemic stroke.

Objective To investigate the therapeutic effect of autologous stem cell transplantation research for patients with cerebral hemorrhage sequelae under the stereotactic.Methods One hundred patients with cerebral hemorrhage from Jan.2011 to Sep.2013 in our hospital were selected and randomly divided into the experimental group (n =50) and the control group (n =50).The patients of experimental group were given autologous stem cell transplantation under the stereotactic in 6 months after cerebral hemorrhage,while the patients in control group were just given traditional treatment.At 6,7 and 12 months after cerebral hemorrhage,rate with neural function defect scale and functional independence measure(FIM) scores of the two groups were compared.Results FIM scores in the experimental group was 102.08 ± 8.28,significant higher than that in control group(95.28±8.75,P＜0.05).Functional independence measure scores in the experimental group at 7 months after cerebral hemorrhage was 13.12±4.00,significant lower than that in control group(20.40±4.33,P ＜0.05).While,there was no statistical difference at 6 months and 12 months after cerebral hemorrhage between the two groups(P＞0.05).Conclusion The therapeutic of autologous stem cell transplantation on patients with cerebral hemorrhage sequelae under the stereotactic is benefit at short term,but the long term therapy effective still needs further study.

During the foreign aid mission against Ebola,the 302 Military Hospital of PLA strengthened emergency science research management according to the features of this task and the reality,and toke effective measures in research direction selection,human resources concordance,scientific potential transformation and other aspects,which contributed to the successful completion of this mission.They gathered experiences in emergency science research management and gave recommendations for similar tasks in the future.

Objective: The compare the safety and efficacy between cryoablation (CRYO) and radiofrequency catheter ablation (RFCA) for treating the patients with atrio-ventricular nodal reentrant tachycardia (AVNRT) by meta-analysis.
Methods: We systemically searched the Medline, Cochrane library and Embase database to fulifll our pre-deifned criteria until the publication of May 2014.
Results: There were 5 randomized controlled trials (RCTs) and 14 retrospective trials enrolled in our study with 2900 patients. The patients were allocated into 2 groups:CRYO group, n=1384 and RFCA group, n=1516. The overall pool-analysis demonstrated that compared with RFCA group, CRYO group had the lower risk of permanent atrio-ventricular nodal block (OR:0.27, 95%CI 0.11 to 0.62, P<0.01) and shorter X-ray exposure time (WMD:-3.36, 95%CI-5.58 to-1.15, P<0.01);while CRYO group had the lower immediate procedural success rate (OR:0.63, 95%CI 0.42 to 0.96, P<0.05), longer procedural time (WMD:10.97, 95%CI 3.35 to 18.58, P<0.01), and higher long-term arrhythmia recurrence rate (OR:2.89, 95%CI 2.05 to 4.06, P<0.01).
Conclusion: Although CRYO could decrease the risk of permanent atrio-ventricular nodal block, while its effectiveness was lower than RFCA for AVNRT treatment in relevant patients.

BACKGROUND:Autologous island flap transplantation is the main therapy for burn scars. Floating and necrosis of transplanted flaps mainly impact the therapeutic effects. Dexamethasone has immunoregulatory function during trauma and stress process. OBJECTIVE:To investigate the effect of dexamethasone on immune balance of Th1/Th2 and prognosis in patients with burn scars undergoing autologous island flap transplantation. METHODS:Sixty-eight patients with burn scars undergoing transplantation of pedicled island flap were selected and randomly divided into control group and experimental group. Patients in the two groups were treated with negative pressure drainage under skin flap, infection prevention, improvement of microcirculation. After that, dexamethasone sodium phosphate (0.2 mg/kg) was immediately given in the experimental group, and the dose gradualy decreased after 3 days until drug withdrawal at 7 days. Levels of Th1, Th2, Th1/Th2 and interferon-γ, interleukin-4 were compared between the two groups before and 1 week after transplantation. In addition, incidence rates of skin flap necrosis, hemodynamic disorder, and effusion were compared between the two groups. RESULTS AND CONCLUSION:The levels of Th1, Th2, Th1/Th2, interferon-γ and interleukin-4 had no obvious differences between the two groups before flap transplantation. At 1 week after transplantation, the levels of Th1 and Th1/Th2 were higher, while the levels of Th2, interferon-γ and interleukin-4 were lower in the experimental group than the control group. Moreover, the incidence of complications was also lower in the experimental group than the control group. In conclude, dexamethasone can decrease the incidence of complications after island flap transplantation, which is maybe the result of adjusting immune balance of Th1/Th2.

Objective To know about the influence of quality control based on Gantt chart on work quality and stress,so as to explore effective method of management suitable for them.Methods 52 nurses from the eastern zone of a three A-level hospital in Jiujiang City were randomly selected as the experimental group,they were implemented with Gantt chart management.Another 52 nurses from the western zone of the same hospital were selected as the control group,they were executed traditional experience-based management.The scores of quality of care and nurses stress were compared between the two groups.Results For the score of the quality of care,the experimental group was higher;,for the score of the nurses stress,the experimental group was lower in the overall sides,and the differences were significant except those of the working environment and equipment,which showed no significant difference between two groups.Conclusions Gantt management method can significantly improve the quality of care,moreover,effectively alleviate the nurses'stress.

Objective To investigate cost and influencing factors of the economic burden of chronic respiratory disease in hospitalize elderly patients. Methods A convenience sample of 284 elderly patients with chronic respiratory disease, investigated with Geriatric Depression Scale (GDS-15) and EuroQol-5 Dimensions (EQ-5D). Results The hospitalization expenses was (8 810.80 ± 4 290.90) yuan,especially medical costs was the highest(54.18%).Correlation analysis and the outcome of multiple lineal stepwise regression reveals that durations of hospitalization (r=0.822), value of GDS-15 (r=0.558), value of EQ-5D (r=0.486), medical insurance types (r=0.290), nutrition medicine (r=0.226), prognosis of disease (r=0.188), measures of PTA (r=0.158), chronic complications (r=0.143) and reimbursement ratio (r=-0.211) were related with the direct economic burden (P<0.05). Conclusion The durations of hospitalization,degree of depression,medical insurance types,complications,nutrition medicine,level of healthy,and the antibacterial effect are the main influence factors of the direct economic burden.Among them,the durations of hospitalization is more severe.

BACKGROUND:Jumonji domain-containing histone demethylase (JMJD) can promote osteoblast differentiation, and estrogen-related receptor alpha (ERRα) can promote osteoblast differentiation and increase bone formation. However, little is reported on the association between postmenopausal osteoporosis andJMJD and ERRα. OBJECTIVE: To study the changes in the JMJD2 family expression in patients with postmenopausal osteoporosis. METHODS: Postmenopausal patients with osteoarthritis of the hip scheduled for total hip arthroplasty, aged 50-70 years, were enroled, including 10 postmenopausal osteoporosis patients (experimental group) and 10 patients with no postmenopausal osteoporosis (control group). During the arthroplasty, the cancelous bone specimens from the femoral head were colected. Then, immunohistochemistry and western blot assay were used to detect expression of histone demethylase (JMJD2A, JMJD2B), histone methylation (H3K9me3, H3K36me3) and ERRα. RESULTS AND CONCLUSION:In the experimental group, the expressions of JMJD2A, JMJD2B and ERRαwere from weakly positive to positive; these expressions were significantly lower in the experimental group than the control group (P < 0.05). The expressions of H3K9me3 and H3K36me3 were significantly higher in the experimental group than the control group (P < 0.05). These findings indicate that the expression of JMJD2A and JMJD2B is consistent with the expression of ERRα in the patients with postmenopausal osteoporosis, and JMJD is likely to serve as an antagonistic enzyme of osteoporosis.

Objective:To establish a GC method for the determination of phenol and L-menthol in glycerin Zhiyang lotions. Meth-ods:A Zebron ZB-WAX(0. 32 mm × 30. 0 m,0. 50 μm) capillary column was used with an FID detector. The column temperature was 60℃, maintained for 1 min, and then raised to 160℃ at the rate of 8℃·min-1 , and maintained 10 minutes. The inlet tempera-ture was 180℃, the detector temperature was 300℃, and the carrier gas was nitrogen. Results:The linear range of phenol and L-men-thol was 0. 5-10. 0 mg·ml-1(r=0. 999 9) and 0. 25-5. 0 mg·ml-1(r=0. 999 9), respectively. The average recovery of phenol and L-menthol was 99. 01%(RSD=0. 90%,n=9)and 99. 70%(RSD=0. 98%,n=9), respectively. Conclusion: The method is sim-ple, accurate and reliable, and can be used to determine the concentration of phenol and L-menthol in glycerin Zhiyang lotions.