Objective To explore the clinical features,related diseases,,etiology of inhalation pneumonia and premonition of lung infection in patients age 80 and over.Methods The 104 patients over 80 years old with pulmonary infection were analyzed retrospectively,statistical analysis of the main clinical manifestations,associated diseases and pathogens.Results Aspiration pneumonia (AP)in 77 cases (74%),Chronic respiratory disease accounted for only 26%,mostly associated with serious cardiovascular and cerebrovascular diseases,cognitive disorder,Chronic gastrointestinal diseases(Gastritis,reflux esophagitis,gastrointestinal dysfunction,constipation),long-term bed,Can not take care of themselves.Sputum bacterial cultures isolated from 66 (63.5%)strains,Gram positive cocci were 18 strains (27.3%),Gram negative bacilli were 38 strains (57.6%),Fungal strains were 15 strains (22.7%).Conclusion In the elderly over the age of 80,pulmonary infection mostly occured by Aspiration pneumonia.We should strengthen the awareness of Aspiration pneumonia,especially accompanied by cough,chronic gastric disease.Take reasonable application of antibiotics according to the pathogen test results.

[Objective] To observe the effect of acupoint sticking at“Zusanli”(ST36) with magnolia powder on patients with acute exacerbations of chronic obstructive pulmonary disease and gastrointestinal dysfunction. [Method] A total of 78 patients with acute exacerbations of chronic obstructive pulmonary disease(AECOPD) and gastrointestinal dysfunction were randomly divided into two groups: treatment group(n=40) and control group(n=38). Al patients received conventional therapy for AECOPD and tablet mosapride 5mg tid for 3 days. Patients in treatment group also received acupoint sticking at“Zu-sanli”(ST36) with magnolia powder q8h for 3 days. [Result]The total effective rate was 75.0%for treatment group,and 44.7%for control group. The total effective rate of treatment group was higher than that of control group, and no marked side effects were found. [Conclusion] Acupoint sticking at“Zusan-li”(ST36) with magnolia powder combined with mosapride can relieve gastrointestinal dysfunction of patients with AECOPD.

To verify the role of introvascular ultrasound(IVUS) in diagnosis of atherosclerosis. MethodsINUS wasused to measure the position, quality and the thickness of the plaque in the elastic artery and biopsy materials. After using HE andHart stains, a micrometer was used to measuse the thickness of plaques and arterious walls. And electron microscopy was used to ob serve the pathological changs of the plaque. ResultsBy histological observation compared with IVUS, the conformable rate of plaque anmounted to 94%. The plaque could be seen in 12 sites of control group. Among 33 sites, 32 plaques were found pathologic exmination while IVUS showed 30 plaques in the experimental group. The two methods had a gcod correlation on the thick measurement of plaque and the media( r = 0.835, P＜0.05 and r = 0.814, P＜ 0.05). But they had a poor correlation on the measurement of the intirna( r = 0.055). ConclusionsBy pathological examination and the quantitative analysis, IVUS can obtain the common purpose in diagnosing atherosclerosis and measuring the media thickness. But it can not be used in measurement of the intima.

Objective To probe the safety of performing transradial artery percutaneous coronary intervention (PCI) for Allen test negative patients.Methods One hundred and six patients performed transradial artery PCI were enrolled in the study.The patients were divided into negative group (57 cases) and positive group (49 cases) according to Allen test results.Patients were performed ulnar artery angiography,deep palmar arch angiography and superficial palmar arch angiography.Ulnar artery diameter,deep palmar arch conditions,superficial palmar arch conditions,the frames counting through angiography on the side of ulnar vessel system,and hand ischemia after PCI (follow up for 3 months) was observed.Results There was no significant difference between negative group and positive group in parameters of ulnar artery diameter [(2.02 ±0.18) mm vs.(2.07 ±0.17) mm] and deep palmar arch and superficial palmar arch [85.96％ (49/57) vs.87.76％ (43/49),75.44％ (43/57) vs.81.63％ (40/49)] (P ＞0.05).The frames counting had no significant difference between negative group and positive group [(218.6 ± 63.6) frames vs.(180.8 ± 44.1) frames],but the ratio of the frames counting ≥380 frames had significant difference between negative group and positive group [14.04％(8/57) vs.2.04％(1/49)] (P ＜ 0.05).None of patients had been found to suffer from hand ischemia after PCI.Conclusion Transradial artery PCI is suitable for Allen test negative patients.

OBJECTIVE:To establish capillary GC method for the determination of residual organic solvents in artesunate,such as methanol and ethyl acetate.METHODS:Capillary GC was adopted and the temperature of flame ionization detector was 250℃.RESULTS:The linear range were 0.03～0.605 mg?mL-1 for methanol(r=0.999 8) and 0.05～1.002 mg?mL-1 for ethyl acetate(r=0.999 7).The average recovery rates were 98.7% for methanol(RSD=3.0%) and 99.1% for ethyl acetate(RSD=2.1%).Only the ethyl acetate was detected in samples.CONCLUSION:Established method is simple and accurate for the content determination of residual organic solvents in artesunate.

The purpose of this study was to observe whether HBV can infect HSC in vitro. LX-2 cells, the human activated HSC cell line, were incubated with human serum containing HBVDNA at final concen-trations from 0.01 to 10 copies/cell and harvested after 24,48 and 72 hours. Hepatitis B surface antigen (HBsAg) and hepatitis B core antigen(HBcAg) were detected by lmmunohistochemistry dyed by DAB. By Immunohistochemistry, no positive particles in LX-2 cells, a few of dark brown particles (HBsAg and HB-cAg) were found in cytoplasm and nucleoli of HepG2.2. 15 cells,a lot of clark brown particles were found in liver biopsies of patients with chronic hepatitis B. In vitro, there was no evidence that HBV can infect and express antigens in LX-2 cells.

Objective To assess myocardial contractility in essential hypertention(EH) of different remoldings by means of circumferential midwall fractional shortening (mFs), relation between mFs and circumferential end-systolic stress(mFs-cEss). Methods The eject fraction(EF), circumferential end-systolic stress(cEss), fractional shortening(Fs), mFs was calculated in 115 EH patients and 29 normal controls. Relations in mFs-cEss, Fs-cEss between the subjects were analysed with linear correlation and linear regression. Results In using EF,Fs,Fs-cEss to assess chamber function of the patients with hypertension of four left ventricular remoldings, it shows apart from eccentric hypertrophy, the rest three remoldings were overestimative. MFs assessing myocardial contractility in four remoldings was lower than that of the controls, especially obvious in concentric hypertrophy patients(P

To review the research of block copolymer micelles as delivery system for poorly soluble(antineoplastic) carrier over the last 10 years,including the composition,preparation methods and factors which influence the loading efficiency,physicochemical properties,and targeting characteristics of block copolymer micelles.Block copolymer micelles are self-assembled structures formed from amphiphilic block copolymers by dispersing in aqueous media.The hydrophilic blocks of the copolymer form the outer shell of the micelle,while the hydrophobic blocks form the inner core,and the proper coreshell micellar architecture was constituted.Block copolymer micelles have a whole set of unique properties,such as small sizes,narrow particle size distribution,high drug loading capacities,and available disposition characteristics in the body.Block copolymer micelles have been found as promising drug carriers due to making poorly soluble antineoplastic lysis,toxicities and side effects decrease,bioavailability increase,and targeting the drugs to specific sites in a passive way or attaching ligands in an active way,which can be specifically(recognized) by receptors onto the surface of copolymers.

Objective To explore the short term effects of five second-generation antipsychotics on the serum pro?lactin levels of first-episode schizophrenia patients. Methods Two hundred fifty first-episode schizophrenia patients were randomly divided into five groups and were then treated with risperidone, olanzapine, paliperidone, quetiapine or ziprasidone, respectively. The serum prolactin were tested at baseline, and every week following initiation of treatment. The positive and negative symptom scale (PANSS) and the treatment emergent symptom scale (TESS) were used to evalu?ate the effect and side effect of treatment. Results Repeated measure ANOVA for serum prolactin showed that the main effects of time, the main effect of group, and the interactive effect of time and group were significant (all P<0.01). At the first week, the serum prolactin level of risperidone group was higher than all the other four groups (P<0.05). At the sec?ond, third, fourth and fifth week, the serum prolactin level of risperidone group and olanzapine group was higher than the other three groups (P<0.05). At the end of the sixth week, the serum prolactin level of risperidone group, olanzapine group and paliperidone group was higher than the quetiapine and ziprasidone groups (P<0.05). But serum prolactin level of risperidone group and olanzapine group was higher than that of paliperidone group (P<0.05). The changes of PANSS before and after treatment were significantly different among groups (P<0.05). However, the incidence of the side effects was not significantly different among groups (P>0.05). Conclusion The level of serum prolactin gradually increases in schizophrenia patients receiving treatment of antipsychotics. The short term effects of different second generation antipsy?chotics on serum prolactin vary differently. Risperidol and olanzapine result in the elevation of serum prolactin level in the early period of treatment.

Objective To study the therapeutic effect of Fumaderm in Freund' complete adjuvant-induced arthritis (AIA) in Spraque-Dawley rats. Methods Adjuvant-induced arthritis (AIA) was established by intradermal injection of 0.1 mL of Freund' complete adjuvant (CFA) in the palmar surface of the right hindpaw and Fumaderm was delivered by oral gavage for 28 days. After CFA injection, the edema of the hindpaw was determined every two days. On 28 days after CFA injection, the lymphocyte subsets of peripheral blood and the cytokines were determined by flow cytometry, meanwhile the histopathological examination of ankle-joints of the animals was performed. Results Fumaderm had a significant therapeutic effect on AIA. The hindpaw swelling was reduced significantly in a dose-dependent manner. The ratio of peripheral blood T lymphocytes was improved obviously. Multiparameter cytokine analysis from peripheral blood CD4+ T cells showed a decrease of proinflammatory cytokines and an increase of anti-inflammatory cytokines in Fumaderm treated animals. A strongly reduced inflammatory response in the joint synovium was observed. Conclusion Fumaderm has potential anti-inflammatory effects on AIA rats. Further investigation is needed to elucidate the molecular mechanism involved in the clinical effect observed in the AIA model.