Objective To investigate expressions of vascular endothelial growth factor (VEGF) and nm23 proteins and microvessel density in HCC,simultaneously,their relation with metastasis was also studied.Methods The expression of VEGF and nm23 and CD34 protein in 56 HCCs were detected by immunohistochemical technique.Results Of 56 HCCs, the intensity of VEGF expression in HCCs with metastasis was significantly higher than that in HCCs without metastasis (P

Objective To evaluate the relationship between relative perfusion rate assessed with power Doppler imaging (PDI) and microvessel density (MVD), as well as expression of vascular endothelial growth factor (VEGF) in bladder carcinoma. Methods The transrectal ultrasound (TRUS) blood flow signal of bladder carcinoma was preoperatively detected with color Doppler imaging in 45 patients, and the relative perfusion rates were obtained for analysis. MVD and the expression of VEGF of excised tumor were assessed immunohistochemically. Results There were correlation between relative perfusion rate and MVD, the expression of VEGF. MVD and the expression of VEGF were related to pathologic grade and the invasiveness of tumor tissues. The expression of VEGF in bladder carcinoma was positively correlated to the tumor interstitial vascular density. Conclusion Combination of PDI and immunohistochemical parameters is useful for evaluating the angiogensis of bladder carcinoma from a different point of view.

Objective To investigate the clinical characteristics and treatment strategies of primary lactase deficiency in infants.Methods The clinical data of 26 infants with primary lactase deficiency in Shenzhen Chidlren's hospital from October 2009 to February 2011 were analyzed retrospectively and the diagnoses were confirmed by dietary therapy.Results The patients were consisted of 19 males and 7 females,whose ages of onset ranged from 1 to 2 months,persisting 1 to 4 months.Breast feeding,mixed feeding and formula feeding were given in 10 cases,8 cases and 8 cases,respectively.The main clinical manifestations were persistent or chronic diarrhea,with watery and or foamy stools,4 to 8 times per day.Nineteen patients had received antibiotics but without improvement.Lactose free formula and oral probiotics improved the symptoms within 1 week in all 26 cases,but recurrence of symptoms followed reintroduction of dairy foods.Conclusion The clinical manifestations of primary lactase deficiency in infants are persistent or chronic diarrhea.Lactose free formula can be used to confirm the diagnosis and to improve the symptoms.

The present investigation was performed in fifteen adult rats using four fluorescent tracers, including 4'-6-diamidino-2-phenylindol. 2 HC1 (DAPI), nuclear yellow (NY), propidium iodide (PI), and fast blue (FB), which were divided into four sets: PI-DAPI, PI-NY, DAPI-NY, and FB-NY. In each set two fluorescent tracers were used separately to label gastric corpus and pylorus antrum. In the medulla oblongata, the labelled cells in the dorsal motor nucleus of vagus (DmnX) were observed in every animal, but those in the nucleus ambiguus (nA) were only seen. in 11 cases and in the nucleus tractus solitarius (nTs) in 5 cases. In addition, labelled cells were also found in laminae V of the posterior grey columns of the thoracic (T6-9) spinal cords, spinal ganglia and sympathetic trunks. The results of counting and analysing the labelled cells of DmnX of both sides of 75 sections in four cases are as follows: 1233 cells on the left, including 1143 labelled cells of gastric corpus (92.7%), 63 labelled cells of pylorus antrum (5.1%), 27 double labelled cells (2.2%); 1007 cells on the right, including 930 labelled cells of gastric corpus (92.3%), 41 labelled cells of pylorus antrum (4.1%), and 36 double labelled cells (3.7%). The results indicate that PI is not fitted for 'long distance labelling in peripheral nervous system; the density of vagus innervation of gastric corpus is larger than that of pylorus antrum; a part of the nerve endings of vagus postganglionic neurons are distributed both in gastric corpus and in pylorus antrum; a part of postganglionic neurons of sympathetic fibres are located at the sympathetic trunk.

The personnel training mode of school-enterprise cooperation, interaction of study and work is the inevitable trend of senior vocational education reform and development at present. The Changsha Social College cooperates closely with rehabilitation agencies and implements the personnel training mode of the “love to help people with disabilities, interaction of study and work, integration of courses and certificate” for training professionals of rehabilitation therapy, has gained some experience and achievements.

OBJECTIVE:To establish a HS-GC method for determining methanol and ethanol in human plasma METHOD_S:An external standard method was used The stationary phase was a glass column packed with GDX-102 The carrier gas was nitrogen The column temperature was 140℃ and both the injector temperature and the detector temperature were 200℃ RE_SULTS:A good linearity was obtained in the range of 0 2～7 5g/L of methanol and ethanol The within-day RSD was less than 4% and the between-day RSD less than 5 5% The recoveries were (100 9?3 8)% for methanol,(101 5?3 4)% for et_hanol,respectively The limits of detection for both methanol and ethanol were less than 0 01g/L CONCLUSION:This method is simple,rapid and precise

Objective To analyze the influence factors on quality of life(QOL) in patients with ovarian cancer in order to provide the basis for improving QOL of patients with ovarian cancer .Methods The quality of life of 73 patients with ovarian cancer was investigated by QLICP‐OV questionnaire .Descriptive statistics ,one‐way analysis of variance ,t‐test and multiple linear regres‐sion analysis were used to explore impact factors of quality of life .Results The total QOL score of 73 patients with ovarian cancer was 64 .85 ± 9 .37 .Single factor analysis indicated that nation ,marital status ,education ,occupation and medical treatments were sig‐nificantly related to some domains scores of the QLICP‐OV(P<0 .05) .The multivariate linear regression analysis indicated that the influence factor on quality of life in patients with ovarian cancer for the domain of physical function was education with regression coefficient being -9 .786;Influence factor for the domain of psychological function was education with regression coefficient being 6 .559;the influencing factors for the specific module were marital status ,medical treatment ,and age with regression coefficients be‐ing -9 .174 ,-7 .142 and 0 .245 ,respectively .Conclusion The quality of life in patients with ovarian cancer was low and it was af‐fected by a lot of factors .

Based on the conservation of nucleotides at splice sites, the characteristics of base composition and base correlation in the adjacent segment sequences, the distance between alternative donor or acceptor splice sites and the content of GC and TC near splice sites, the donor and acceptor splice sites for alternative and constitutive introns are predicted by use of the method of diversity measure combined with quadratic discriminant analysis. For alternative splice sites the total prediction accuracies are 87.9% and 89.9% for donors and acceptors respectively (with the chosen threshold -2). For constitutive splice sites the total accuracy are 92.8% and 94.3% for donors and acceptors respectively (with the chosen threshold -1).

Objective To assess the short-time economics of various glutamine dipeptide-enriched parenteral nutrition (PN) for patients undergoing elective surgery for gastrointestinal tumors, with an attempt to provide evidence for decision makers on clinical nutrition support.Methods A prospective cohort study was designed.From payer/disburser''s perspective, a cost-effectiveness decision-tree model was developed to assess the clinical outcomes and short-time economic effects of glutamine dipeptide-enriched PN that used in different time points (early postoperative or perioperative).Cost-effectiveness analysis, cost-utility analysis, and incremental cost-effectiveness analysis were adopted in the decision-tree model.One-way sensitivity analysis was performed to determine the robustness of the results.Results Totally 107 patients were included.There was no significant difference between the perioperative alanine(Ala) glutamine(Gln) nutrition support (group A) and early postoperative Ala-Gln nutrition support (group B) in the ratio of 5％ weight declines on the 8th day after surgery and infection-related postoperative complications (72.1％ vs.78.1％, χ2=0.509, P=0.498 and 2.32％ vs.4.69％, χ2=0.060, P=0.806).The levels of prealbumin (PA) and albumin(Alb) and the level of total lymphocyte count(TLC) also the time of recovering gastrointestinal function, length of stay nutritional discharge index(LOSNDI), and direct costs were significantly different [PA:(208.19±56.92)mg/L vs.(187.97±62.05)mg/L, t=2.283,P=0.039;Alb:(33.82±3.91)×109 vs.(31.96±4.57)×109, t=2.184, P=0.036;TLC:(1.19±0.55)×109 vs.(0.89±0.66)×109, t=2.461, P=0.015;the time of recovering gastrointestinal function(3.06±0.28)d vs.(3.39 ± 0.34)d, t=-3.675, P=0.000;LOSNDI:(16.84±2.92)d vs.(18.52 ±3.47)d, t=-2.613, P=0.011;direct costs:￥(17 029.05±317.28) vs.￥(15 610.64±292.56), t=23.764, P=0.000].When LOSNDI and quality-adjusted life years (QALYs) were estimated as indicators of effectiveness, the incremental cost-effectiveness ratios and incremental cost-utility ratios of group A were ￥844.3 and ￥70 920.5, respectively.Net monetary benefit of group B was more than that of group A.One-way sensitivity analysis showed that parameters had no significant effect on the model.Conclusion When using local per capita gross domestic product as threshold, early postoperative Ala-Gln PN was more economical than perioperative Ala-Gln PN strategy evaluation.