Objective To study the expression of cIAP and Caspase‐7 in endometrial adenocarcinoma tissue and its relation with prognosis .Methods Fifty‐five cases tissues of endometrial adenocarcinoma patients were detected by immunohistochemistry assay from the First Affiliated Hospital of Henan University of Science and Technology from 2009 to 2010 .Histological classifica‐tion ,pathological stage ,lymph node metastasis ,age and other parameters were collected ,the survival and overall survival of the tumor were followed up ,immunohistochemistry was used to observe the expression of cIAP and Caspase‐7 in paraffin sections of pathological specimens ,and the relationship between them and the parameters were analyzed .Results The expression level of cIAP and Caspase‐7 was related to the histological classification ,pathological stage and lymph node metastasis of endometrial carcinoma (P<0 .05) ,but it was not related with the age ,muscular layer infiltration(P>0 .05) .There was significant difference between the positive expression and the negative expression of cIAP and Caspase‐7(P<0 .05) .Conclusion The expression level of cIAP and Caspase‐7 in patients with endometrial carcinoma is correlated with tumor grade and overall survival ,or can be used as a prognostic indicator for endometrial cancer .

Objective To explore the relationship between angiotensin I-converting enzyme(ACE)gene I/D and angiotensin Ⅱ type 1 receptor(AT1R)gene A1166C polymorphisms and cereral infarction(CI).Methods ACE and AT1R genotypes were investigated with the method of PCR-RLFP in 88 patients with CI and compared with 90 age-matched population controls.Results AC genotypic frequency(31.8%)and C allele frequency(15.9%)of AT1R gene in CI group were significently higher than those in control group(11.1%,5.6%)(all P0.05).Conclusions The polymorphism of AT1R A1166C is related to the incidence of CI.There are synergistic effects of ACE DD genotype and AT1R gene A1166C polymorphisms on the risk of CI.

Objective To investigate the relationship between polymorphisms of angiotensinogen(AGT) gene T704C and cerebrovascular disease(CVD).Methods AGT T704C genotype and allele were examined by PCR-RLFP in 82 patients with cerebral infarction(CI group),70 patients with intracerebral hemorrhage(ICH group) and 89 age-matched normal controls(NC group).The AGT T704C genotype and allele frequencies among the 3 groups were compared and analyzed.The effectes of AGT T704C genotype and the risk factors of stroke to induce CI snd ICH were analyzed by Logistic regression. Results The frequencies of AGT 704CC genotype and C allelic in CI group(63.4%,79.9%) were significantly higher than those in NC group(34.8%,61.2%)(allP

Objective To disccuss the antioxidative effects of sodium ozagrel(OZG) in myocardial ischemia in rats and illuminate its effect of decreasing oxidative damage.Methods Forty healthy Wistar rats were randomly devided into five groups:control,model,pretreatment,treatment and therapeutic alliance groups.OZG was used for 2 weeks in pretreatment group,then isoprenaline hydrochloride (ISO) was applied to make myocardial ischemia model.5 min after the establishment of model,OZG and OZG combined with glyceryl trinitrate(GT) were used in treatment group and therapeutic alliance group,respectively,ECG was recorded at the same time.1 h later,the activities of serum myocardial creatases(AST,LDH,CK),malonaldehyde(MDA) level and superoxide dismutase(SOD) activity were detected.Results ECG:When the model was successfully set up,ST segment in every group all raised≥0.1 mV,the minimum value of raise was in pretreatment group.When the model was succeeded 5 min later,ST segment in model group slightly decreased but still ≥0.1 mV and it was significant in pretreatment group.In treatment and therapeutic alliance groups,compared with model succeeding'ST segment,ST segment decreased significantly at 5 min later(P

This article selected several typical clinical cases, analyzed many factors affect the doctor-patient relationship of ethical issues and new technology of differentiating the ethical dilemma for clinical application, and put forward some suggestions for the reference of medical staff , hospital ethics committees and health policy makers.

With the constant deepening of the study in the genetic factors of eardio-cerebrovascular diseases, the relation between the renin-angiotensin system (RAS) gene polymorphisms and hypertension is increasingly receiving attention. As an important component of RAS, renin has received much concern in the genetic research of cardio-cerebrovascular diseases and its gene polymorphisrns have become the candidate genes of hypertension, coronary heart disease and stroke, etc.

Objective Curculiginis extract has good scavenging effects on oxygen free radicals, and there is no curculiginis extract for AD research at home.The aim of this study was to explore the influence of Curculigoside on the cognitive learning and memory functions of senile dementia model rats induced by D-galactose combined with β-protein amyloid β25-35 (Aa25 35) and the expression level of hippocampus B-cell lymphoma-2 (Bcl-2), Bax, estrogen receptor (ER) protein and mRNA.Methods Forty SD rats weredivided into the control group (equal saline was intraperitoneal injected for six weeks consecutively, and equal saline was injected to the bilateral hippocampus in the seventh week), the model group (galactose was intraperitoneal injected for six weeks consecutively, and 4 mmol/L Aβ25-35 was injected to the bilateral hippocampus in the seventh week), the low dosage group and the high dosage group randomly (the stomach irrigation of low dosage 24mg/kg or the high dosage 72mg/kg curculigoside was applied every day, for 8 weeks), 10 rats in each group.After the treatment , the Morris water maze was conducted to test the space learning and memory functions of rats;the flow cytometry method was conducted to analyze the neuronal apoptosis conditions;the western blot was conducted to detect Bcl-2, Bax and ER protein level;the Realtime PCR was conducted to detect Bcl-2, Bax and ER mRNA level.Results Morris water maze test results: Compared with the model group, the escape latency (EL) of the rats of the model group, the low dosage group and the high dosage group on the 2nd, the 3rd, the 4th and the 5th day significantly extends (P<0.01), and the high dosage group significantly shortens (P<0.05) than the low dosage group.Space exploration test results: Compared with the proportion of the distance swum within the quadrant by the rats in the model group in the total distance [(30.14±5.10)%], that of the low dosage group, the high dosage group and the control group [(41.98±4.78)%, (46.17± 9.31)% and (50.13±6.81)%] significantly increases (P<0.01);in addition, the high dosage group is higher than the low dosage group (P<0.05).Compared with the apoptosis rate of rates in the model group[(7.11±1.78)%], that of the high dosage group, the low dosage group and the control group[(5.53±1.45)%], (4.11±1.03)% and (3.08±1.33)% significantly decreases (P<0.05).Compared with the control group, the Bcl-2 and Bax protein expression of the model group significantly increases, and the cell Bcl-1 and Bax of cells significantly decreases (P<0.05).Compared with the model group, the Bcl-2 and ER protein expression as well as the Bcl-2/Bax of the high dosage group and the low dosage group significantly increase, and the Bax protein expression significantly decreases (P<0.05).Compared with the mRNA expression (1.00 and 1.00) of Bcl-2 and ER in the control group, the mRNA expression (1.31 and 1.98) of the Bcl-2 and Bax in the model group significantly increases (P<0.05);compared with the mRNA expression (1.13 and 1.08) of Bcl-2 and ER in the model group, the Bcl-2 (1.95 and 2.01) and mRNA expression (1.81 and 1.96) of ER of the high dosage group and the low dosage group significantly increase (P<0.01).Conclusion Curculigoside plays an improving role in space learning and memory functions of senile dementia model rats, with the mechanism may be related to the protection to the hippocampus neurons by restraining Bax expression and neuronal apoptosis through the up-regulation of hippocampus ER and Bcl-2 expressions.

Background:miR-181b is related to the progression of many tumors,however,its expression in colitis-associated colon cancer is not clear. Aims:To investigate miR-181b expression and its potential target genes in the process of colitis-associated colon carcinogenesis. Methods: Colitis-associated colon cancer model was established by combined administration of azoxymethane(AOM)and dextran sulfate sodium(DSS)in mice. The expression of miR-181b in colon tissue at different time points was measured by real time fluorescent quantitative PCR(qPCR). Target genes of miR-181b were screened preliminarily by genome microarray combined with miRNA target gene prediction softwares TargetScan, PicTar and miRDB. Expressions of target genes in colitis-associated colon cancer mice were detected by qPCR. Results:The expression of miR-181b was gradually elevated during the development of colitis-associated colon cancer. However, AOM or DSS alone did not change expression of miR-181b. Genome microarray combined with miRNA target gene prediction softwares showed that Ipmk,E2f5,Klf6,Prkcd,Bai3,Hic2 might be the target genes of miR-181b. qPCR showed that expressions of Ipmk,Prkcd,Bai3,Hic2 were significantly decreased in colitis-associated colon cancer than in control group. Conclusions:Expression of miR-181b in colon is upregulated with the development of colitis-associated colon cancer,but is irrelevant with simple chronic inflammation.

Objective To investigate the relationship between renin (REN) gene G10631A, angiotensinogen (AGT) gene T704C mononucleotide polymorphisms and cerebral infarction and to investigate the mechanisms and characteristics of cerebral infarction from molecular level. Methods REN gene G1063A and AGT gene T704C polymorphisms in 82 patients with cerebral infarction and 89 controls were detected with polymerase chain reactionrestriction fragment length polymorphism. The differences of the genotypes and allele frequencies were compared between the patient group and the control group. Results The frequency of REN 10631AA genotype (31. 7% vs. 10. 1%,χ2 =12. 816, P = 0. 002) and the frequency of A genotype (49. 4% vs. 30. 3% χ2 = 12. 969, P =0. 000), as well as the frequency of AGT 704 CC genotype (63. 4% vs. 34. 8% χ2 = 15. 029, P = 0. 001) and the frequency of A genotype (79. 9% vs. 61. 2% χ2 = 14. 173, P = 0. 000) in the cerebral infarction group were all significantly higher than those in the control group; the frequency of haplotype 704C 10631A was also significantly higher than that in the control group (P=0. 000). Conclusions REN 10631AA genetype and A allele as well as AGT 704 CC genetype and C allele may be the susceptible factors of cerebral infarction. Haplotype 704C-10631 A may be a genetic risk factor for the occurrence of cerebral infarction.

Objective:To investigate the efficacy and safety of laparoscopic versus open surgery for early cervical cancer and analyze the factors that influence laparoscopic surgery for early cervical cancer. Methods:The clinical data of 411 patients with International Federation of Gynecology and Obstetrics (FIGO)stage IA1-IIA2 cervical cancer who received treatment in Liuzhou Worker's Hospital, China between January 1, 2000 and August 31, 2016 were retrospectively analyzed. The clinical data were divided into laparoscopic surgery ( n = 320) and open surgery ( n = 91) groups according to surgical approaches. The operation time, intraoperative injury, intraoperative blood loss, time to first postoperative anal exhaust, postoperative complications, time to extubation, and postoperative recurrence rate were compared between laparoscopic surgery and open surgery groups. Multivariable COX regression analysis was performed to analyze the recurrence of cervical cancer after laparoscopic surgery. Results:The operation time in the laparoscopic surgery group was significantly shorter than that in the open surgery [(223.4 ± 53.2) min vs. (251.0 ± 50.0) min, t = 20.200, P < 0.05]. The intraoperative blood loss, time to extubation and the time to first postoperative anal exhaust in the laparoscopic surgery group were less or shorter than those in the open surgery group (all P < 0.05). Under the premise that there were no significant differences in vascular and lymphatic space invasion, tumor size, parametrial invasion, depth of muscle invasion, lymph node metastasis, and vaginal margin between the laparoscopic surgery and open surgery groups, the 1-year and 3-year recurrence rate of cervical cancer was 9.4% (30/320) and 15.9% (51/320), respectively and the 1-year and 3-year tumor-free survival rate was 90.6% and 84.1%, respectively in the laparoscopic surgery group; the 1-year and 3-year recurrence rate of cervical cancer was 4.4% (4/91) and 9.9% (9/91), respectively and the 1-year and 3-year tumor-free survival rate was 95.6% and 89.6%, respectively in the open surgery group. There were no significant differences in 1-year and 3-year recurrence rate of cervical cancer and 1-year and 3-year tumor-free survival rate between laparoscopic surgery and open surgery groups (all P > 0.05). During the 5-year follow-up, there were no significant differences in the recurrence rate of cervical cancer (18.7% vs. 12.2%) and the tumor-free survival rate (81.9% vs. 86.8%) between the laparoscopic surgery and open surgery groups (both P > 0.05). Univariate and multivariate analysis showed that positive lymph node metastasis, tumor size ≥ 2 cm, FIGO stage > IB1 and non-squamous cell carcinoma pathological type are the risk factors for the recurrence of cervical cancer. Conclusion:Laparoscopic surgery for early cervical cancer can reduce the intraoperative and postoperative complications. Positive lymph node metastasis, tumor size ≥ 2 cm, FIGO stage > IB1, and non-squamous cell carcinoma pathological type are the risk factors of recurrence of cervical cancer after laparoscopic surgery.