Objective To establish a method of HPLC for determination of salidroside in Jintianquban Tablets. Methods Waters C18 column was used. The methanol-water was used as the mobile phase. The flow rate was 0.8 mL/min. The detection wavelength was set at 276 nm. Results For salidroside, the linear range was 0.3 456~3.456 ?g, and the average recovery rate was 100.3% with RSD=1.71%. Conclusion The method is simple, reliable, accurate and can be applied to the quality control of the preparation.

OBJECTIVE: To investigate the effect of aerobic exercise on immune factor IL-4, TNF-alpha in patients with allergic rhinitis, which provide a theoretical basis for the treatment of allergic rhinitis. METHOD: Thirty-two allergic rhinitis patients were selected and divided into a control group (14 people) and the experimental group (18 people). During the treatment, both groups receive conventional symptomatic treatment. Meanwhile, aerobic exercise therapy is applied to the experimental group for 40 - 60 minutes a day, 3 - 4 days a week. Serum IL-4, TNF-alpha and allergic rhinitis symptoms graded scoring were tested before and after 6 month therapy. RESULT: After six months of exercise intervention, the 11-4, and TNF-alpha levels of experimental group were significantly lower than the level of control group. The symptoms score is also significantly decreased in experimental group. CONCLUSION Aerobic exercise had good therapeutic effect in patients with allergic rhinitis. The mechanism may be that aerobic exercise can reduce the level of IL-4 and TNF-alpha, which can relieve infiltration and activation of inflammatory cells.
Adolescent ; Adult ; Exercise ; Exercise Therapy ; Female ; Humans ; Interleukin-4 ; blood ; Male ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; blood ; therapy ; Tumor Necrosis Factor-alpha ; blood ; Young Adult

Objective To investigate the clinical significance of high mobility group box-1 protein (HMGB1) in chronic,severe hepatitis B patients and liver cirrhosis.Methods The contents of HMGB1 was measured by enzyme-linked immunoassay (ELISA) in the patients (include 40 patients with chronic hepatitis B and 18 severe hepatitis B patients and 18 liver cirrhosis patients) and 20 health controls.The levels of relative biochemical indicators,prothrombin time activity percentage (PTA) and the hepatic fibrosis were determined by biochemical methods.Results The contents of HMGB1 was significantly increased in severe hepatitis patients in comparison with that in chronic hepatitis B patients(P ＜0.01).The contents of HMGB1 in the chronic hepatitis were significantly increased in health controls(P ＜0.01).The contents of HMGB1 had significant difference among the liver cirrhosis patients,the severe hepatitis patients and the chronic hepatitis(P ＜0.01).There was positive correlation among the contents of HMGB1 and total bilirubin (TBIL),the proportionality of aspartate aminotransferase and alanine aminotransferase (AST/ALT),hyaluronic acid (HA) and procollagen Ⅲ N-terminal peptide (P Ⅲ NP) (r = 0.865,0.646,0.783,0.662,P ＜ 0.01).There was negative correlation among the contents of HMGB1 and prothrombin time activity percentage (PTA) and albumin (ALB) (r =-0.915,-0.852,P ＜0.01).Conclusion The contents of serum HMGB1 were closely associated with disease severity in chronic hepatitis B patients.HMGB1 was an index to auxiliary diagnosis hepatic fibrosis.

Objective To analyze the coverage of iodized salt and the focus population iodine nutrition after implementing new standard of iodized salt in Hainan Province.Methods Eighteen cities (counties,districts) were divided into eastern coastal,western coastal and inland areas according to coastline and terrain of Hainan Province in 2013.One township (town,street) was sampled from each city (county,district) in the east,the west,the south,the north and the center of these areas.Four villages (committees) and 15 households in each village (committee),and 40 children aged 8-10 in one primary school,20 pregnant women and lactating women were sampled from each township (town,street).Iodized salt samples collected from household were monitored by the method of direct titration;random urine samples of children aged 8-10,pregnant woman and lactating woman were tested by arsenic cerium catalytic spectrophotometry.Results Totally 6 310 edible salt samples were tested,and the median of salt iodine was 25.9 mg/kg.The coverage of iodized salt was 97.92％ (6 179/6 310).Residents consumption rate of qualified iodized salt reached 95.47％ (6 024/6 310),and the rate of non-iodized salt was 2.08％ (131/6 310).About 4 444 urine samples of children aged 8-10 were examined,and the median of urinary iodine was 165.4 μg/L,in which suitable urinary iodine (100-199 μg/L) accounted 49.3％ (2 194/4 444);about 1 286 urine samples of lactating woman were examined,the median of urinary iodine was 122.3 μg/L,≥ 100 μg/L was 60.6％ (779/1 286);about 813 urine samples of pregnant woman were examined,the median of urinary iodine was 126.6 μg/L,59.6％ (485/ 813) of this group were urinary iodine deficiency (＜ 150 μg/L).There were significant differences between the three groups in the east,the middle and the west areas of Hainan Province (children:176.7,147.7,158.0 μg/L,lactating women:148.7,108.7,106.7 μg/L,pregnant women:146.7,108.0,147.1 μg/L,H =34.70,26.76,35.95,all P ＜ 0.01).Conclusion The results have showed that pregnant woman are iodine deficiency,they should be given more iodine.

Objective Curculiginis extract has good scavenging effects on oxygen free radicals, and there is no curculiginis extract for AD research at home.The aim of this study was to explore the influence of Curculigoside on the cognitive learning and memory functions of senile dementia model rats induced by D-galactose combined with β-protein amyloid β25-35 (Aa25 35) and the expression level of hippocampus B-cell lymphoma-2 (Bcl-2), Bax, estrogen receptor (ER) protein and mRNA.Methods Forty SD rats weredivided into the control group (equal saline was intraperitoneal injected for six weeks consecutively, and equal saline was injected to the bilateral hippocampus in the seventh week), the model group (galactose was intraperitoneal injected for six weeks consecutively, and 4 mmol/L Aβ25-35 was injected to the bilateral hippocampus in the seventh week), the low dosage group and the high dosage group randomly (the stomach irrigation of low dosage 24mg/kg or the high dosage 72mg/kg curculigoside was applied every day, for 8 weeks), 10 rats in each group.After the treatment , the Morris water maze was conducted to test the space learning and memory functions of rats;the flow cytometry method was conducted to analyze the neuronal apoptosis conditions;the western blot was conducted to detect Bcl-2, Bax and ER protein level;the Realtime PCR was conducted to detect Bcl-2, Bax and ER mRNA level.Results Morris water maze test results: Compared with the model group, the escape latency (EL) of the rats of the model group, the low dosage group and the high dosage group on the 2nd, the 3rd, the 4th and the 5th day significantly extends (P<0.01), and the high dosage group significantly shortens (P<0.05) than the low dosage group.Space exploration test results: Compared with the proportion of the distance swum within the quadrant by the rats in the model group in the total distance [(30.14±5.10)%], that of the low dosage group, the high dosage group and the control group [(41.98±4.78)%, (46.17± 9.31)% and (50.13±6.81)%] significantly increases (P<0.01);in addition, the high dosage group is higher than the low dosage group (P<0.05).Compared with the apoptosis rate of rates in the model group[(7.11±1.78)%], that of the high dosage group, the low dosage group and the control group[(5.53±1.45)%], (4.11±1.03)% and (3.08±1.33)% significantly decreases (P<0.05).Compared with the control group, the Bcl-2 and Bax protein expression of the model group significantly increases, and the cell Bcl-1 and Bax of cells significantly decreases (P<0.05).Compared with the model group, the Bcl-2 and ER protein expression as well as the Bcl-2/Bax of the high dosage group and the low dosage group significantly increase, and the Bax protein expression significantly decreases (P<0.05).Compared with the mRNA expression (1.00 and 1.00) of Bcl-2 and ER in the control group, the mRNA expression (1.31 and 1.98) of the Bcl-2 and Bax in the model group significantly increases (P<0.05);compared with the mRNA expression (1.13 and 1.08) of Bcl-2 and ER in the model group, the Bcl-2 (1.95 and 2.01) and mRNA expression (1.81 and 1.96) of ER of the high dosage group and the low dosage group significantly increase (P<0.01).Conclusion Curculigoside plays an improving role in space learning and memory functions of senile dementia model rats, with the mechanism may be related to the protection to the hippocampus neurons by restraining Bax expression and neuronal apoptosis through the up-regulation of hippocampus ER and Bcl-2 expressions.

OBJECTIVE To investigate the drug susceptibility of clinical isolates in local region for using antibiotic reasonably. METHODS Totally 3 170 strains of clinical isolates were identified by API and Microscan and tested for drug resistance against antimicrobial agents by K-B method. WHONET5.4 was applied for analysis. RESULTS The commonly encountered bacteria were Pseudomonas aeruginosa (24.3%), Acinetobacter baumannii (10.9%), Klebsiella pneumoniae (10.4%), Escherichia coli (8.9%), and Staphylococcus aureus (SA,8.0%). In Gram-negative isolates, the resistance rate to meropenem was 19.7%, and to piperacillin-tazobactam was 26.5%. The incidences of E.coli and K. pneumoniae isolates producing extended spectrum beta-lactamases (ESBLs) were 49.1% and 33.5%, respectively. In Gram-positive isolates, the susceptibility rate to vancomycin and teicoplanin both was 100.0%. The oxacillin resistant rates of SA and coagulase negative Staphylococcus (CNS) were 54.2.0% and 82.3%. CONCLUSIONS The production ratio of ESBLs and oxacillin resistance of bacteria in local region are high. It is important to promote the rational use of antimicrobial agents and take effective contaminant methods to reduce resistant rates of bacteria and dissemination of multi-resistant bacteria.

Objective To establish a mouse model of H.pylori infection, and to evaluate the chronic pathological changes in the gastric mucosa associated with H.pylori infection.Methods 34 male 5~6-week old SPF C57BL/6 mice were used in this study.The mice were intragastrically administrated with a suspension of H.pylori SS1 strain.Two weeks after infection, rapid urease test and PCR were performed to confirm the H.pylori infection.Successfully infected mice were randomly divided into 3 groups including the control group, 6-week and 12-week infected groups.Samples of gastric mucosa were taken for pathological analysis using HE and borax methylene blue staining.The contents of myeloperoxidase (MPO), superoxide dismutase (SOD), malondialdehyde (MDA) and catalase (CAT) in the gastric tissues were detected by biochemistry, and the expression levels of COX-2, iNOS, TNF-α and IL-1β were examined by RT-qPCR.Results Compared with the control group, H.pylori colonization was observed in the gastric mucosa of the 6-week and 12-week infected mice, with chronic inflammatory cell infiltration, glandular atrophy and intestinal metaplasia to varying extents.The contents of CAT and SOD were significantly decreased, while the levels of MPO and malonaldehyde MDA, and the expression levels of COX-2,iNOS,TNF-α and IL-1β were significantly increased (P<0.05 or P<0.01).Conclusions Intragastric administration with H.pylori in C57BL/6 mice can be successfully used to generate the bacterial colonization, leading to chronic inflammatory cell infiltration, enhanced oxidative stress, and up-regulated expression of proinflammatory genes in the gastric glandular tissues at 6 and 12 weeks after inoculation.However, the inflammatory changes are more extensive in the mice at 12 weeks after infection, with glandular atrophy and intestinal metaplasia.

Objective To investigate the effect of Tongxinluo on carotid artery stenosis,and the levels of high sensitive C reactive protein(hs-CRP) and D-Dimer in patients with cerebral infarction.Methods 168 patients with cerebral infarction treated in this hospital from June 2014 to June 2015 were randomly divided into experimental group and control group,with 84 cases in each group.The patients in both group received routine treatment.In addition to that,patients in the experimental group also used Tongxinluo capsule for 6 months as a course of treatment.After a course of treatment,Doppler ultrasound diagnosis instrument was used to detect carotid arterial intima-media thickness,plaque area and carotid artery stenosis rate.Hs-CRP and D-Dimer levels were also detected.Results After treatment,the carotid arterial intima-media thickness,plaque area and carotid vascular stenosis rate in two group were significantly decreased(P<0.05),but the three indicators in experimental group were decreased more significantly(P<0.05).After a course of treatment,the levels of D-Dimer and hs-CRP in peripheral blood of the two groups were significantly decreased(P<0.05),but the content of D-Dimer and hs-CRP was much lower in the experimental group(P<0.05).Conclusion Tongxinluo treatment could effectively reduce the level of hs-CRP and D-Dimer in patients with cerebral infarction,and alleviate the carotid artery stenosis,which might be worthy of clinical application.

In order to develop a combined live vaccine that will be used to prevent against porcine parvovirus (PPV) and Pseudorabies virus (PRV) infection, the VP2 gene of PPV was inserted into the transfer vector plasmid pG to produce the recombinant plasmid pGVP2. The plasmid pGVP2 and the genome of PRV HB98 attenuated vaccine were transfected by using lipofectamine into swine testis cells for the homologous recombination. The recombinant virus rPRV-VP2 was purified by selection of green fluorescence plaques for five cycles. 6-week-old female Kunming mice were immunized intramuscularly with attenuated PRV parent HB98 strain, commercial inactivated vaccine against PPV, recombinant virus, DMEM culture solution. The injections were repeated with an equivalent dose after 2 weeks in all of the groups, and then challenged with the virulent PRV NY strain at 7 weeks after the first immunization. The recombinant virus rPRV-VP2 was successfully generated, and the recombinant virus could effectively elicite anti-PPV and PRV antibody and significant cellular immune response as indicated by anti-PPV ELISA and HI, PRV-neutralizing assay and flow cytometry. The challenge assay indicated that recombinant virus could protect the mice against the virulent PRV challenge. These results demonstrated that the recombinant virus can be a candidate recombinant vaccine strain for the prevention of PRV and PPV.
Animals ; Antibodies, Viral ; immunology ; Antigens, Viral ; administration & dosage ; genetics ; immunology ; Capsid Proteins ; administration & dosage ; genetics ; immunology ; Female ; Gene Expression ; Genetic Vectors ; genetics ; metabolism ; Herpesvirus 1, Suid ; genetics ; metabolism ; Mice ; Parvovirus, Porcine ; genetics ; immunology ; Swine ; Swine Diseases ; immunology ; prevention & control ; virology ; Viral Vaccines ; administration & dosage ; genetics ; immunology

Objective　To evaluate the therapeutic effect of rapid angulation rotation traction for lumbar intervertebral disc protrusion. Methods　A total of 88 patients (66 male and 22 female;age: 18-65), with a history of 2 days to 10 years were analyzed. Among them 20 cases were central protrusion 68 cases were lateral protrusion. Traction range: 60-65 mm, angle: 20°-25°, rotation degree: 20°-25°, traction time: 3 s-1 min. The process of treatment was computer designed. The traction was then 1 to 3 times, with a interval of 4-7 days between two treatments. Results　The effective rate was 96% for the case with history <1 month 96% and 85% for cases with history >1 month (P<0.01). The effective rate was 62% for central protrusion, and 87% for lateral protrusion respectively. χ2 test showed the difference was not significant. Conclusion　The rapid angulation rotation is an effective treatment for lumbar inter-vertebral disc protrusion and this non operative method should be used as early as possible.