Objective To evaluate the effect of trimetazidine in ischemic cardiomyopathy patients with impaired systolic function. Methods There were 90 cases in total, and were divided into two groups randomly: 42patients took conventional therapy,and 48 patients took trimetazidine additionally. Plasma levels of brain natriuretic peptide (BNP), left ventricular ejection fraction(LVEF) and left ventricular end-diastolic dimension(LVEDD) were measured before and after trimetazidine therapy at 6 months, 12 months. Results Compared to conventional therapy group, LVEF, LVEDD and BNP were significantly improved in the trimetazidine group (P ＜ 0.01 ). Conclusions Trimetazidine additional to conventional therapy might improve heart function and reverse ventricles reconstitution in ischemic cardiomyopathy patients with impaired systolic function.

OBJECTIVE:To study the protective effect of ethyl acetate extract from Oenanthe stolonifera against myocardial ischemia and ischemia reperfusion injury in rats.METHODS:Acute myocardial ischemia and ischemical reperfusion injury models were prepared by suture-occluded method.5 min before the onset of ischemia,the rats were injected with ethyl acetate extract from O.stolonifera,then the dynamic ECG change of rats was observed,and the arrhythmia caused by myocardial ischemia and the myocardial infraction size,the arrhythmia induced by myocardial ischemia-reperfusion,the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in plasma were determined.RESULTS:Ethyl acetate extract from O.stolonifera significantly inhibited myocardial ischemia-induced arrhythmia and decreased the myocardial infarction size,significantly inhibited arrhythmia induced by myocardial ischemia-reperfusion,enhanced SOD activity and decreased the content of MDA in plasma.CONCLUSION:The protective effect of ethyl acetate extract from O.stolonifera against myocardial ischemia and ischemical reperfusion injury in rats is possibly associated to its anti-peroxidation effect.

Objective To study the effect of angiotensin II in its different concentrations and different action time on VEC apoptosis and the expression of Fas, Bcl-2 in vascular endothelial cells (VEC) and to study the influences of enoxaparin on the above roles. Methods Flow cytometer was used to measure the apoptosis ratio and the expression of Fas, Bcl-2 which were induced by angiotensin II in its different concentrations and different action time, and influenced by enoxaparin. Results Apoptosis ratio(1.86?0.18,30.62?4.20;1.67?0.15,30.08?4.41)and the expression of Fas(1.13?0.16,14.85?1.47;1.13?0.16,15.67?1.97), Bcl-2 (2.38?0.43,9.62?1.81;2.38?0.43,12.33?1.73)were induced and increased by angiotensin II with the increase of concentrations and action time. Enoxaparin had an effect on apoptosis ratio and the expression of Fas, Bcl-2, which were induced by angiotensin II(30.62?4.20,18.31?2.64;14.85?1.47,7.28?1.36;9.62?1.81,16.05?1.79). Conclusions Apoptosis ratio and the expression of Fas, Bcl-2 may be induced and increased by angiotensin II with the increase of the concentrations and the action time. Enoxaparin may have the reduce effect on apoptosis ratio and the expression of Fas and Bcl-2 in vascular endothelial cells.

In order to improve nursing informationization,and to promote the development of nursing informatics, our nursing department set up a new position called informatics nurse and a series of working systems and man-agement methods were developed. After implementation,the informatics nurse had achieved various goals,including:playing a role in developing 60 nursing information system programs,improving 11 medical and nursing process modification,collecting 399 items of nursing information system problems with 338 items solved,completing 22 nurs-ing information system using instructions,and organizing training meetings and lectures in the hospital for 12 times. The informatics nurse has been appreciated by medical staff and IT engineers who was believed to have promoted the development of nursing informatics in our hospital.

A major task in clinical trials and researches is subject recruitment, however there probably exist ethical issues in the process of recruitment, such as the selection of subjects, recruitment methods, document de-scription,reward and compensation. This paper is to discuss the relevant ethical elements through cases analysis. It put forward that only obeying ethical codes, implementing informed consent, and playing the function of ethics com-mittee, the rights and interests of subjects can be protected.

Objective To explore the effect of Yi Xuesheng Jiaonang combined with Iron Sucrose Injection on anemia in pregnancy on serum transferrin receptor and pregnancy outcome.Methods 78 patients with anemia in pregnancy form December 2014 to December 2015 of the third people’s Hospital of Cixi were collected and randomly divided into control group and treatment group,each had 39 cases.Control group was given 200 mg Iron Sucrose Injection with 100 mL 0.9%sodium chloride injection intravenous infusion,2 times a week; experiment group were treated on the base of control group with 4 pills of Yi Xuesheng Jiaonang,3 times daily.The course was 28d,and two groups all with a course treatment.After the end of treatment,clinical curative effect,serum transferrin receptor level,liver and renal function and the incidence of adverse pregnancy outcome were observed and compared. Results Serum transferrin receptor levels were increased in two groups after treatment (P<0.05),compared with control group,serum transferrin receptor level of treatment group was even higher (P<0.05), alanine aminotransferase (ALT),aspartate aminotransferase (AST),serum creatinine (Cr) and urea nitrogen ( BUN) and other indicators of liver and kidney function of experiment group had no statistical significance.Adverse pregnancy outcome rate betwen two groups was not statistically significant.Conclusion Yi Xuesheng Jiaonang combined with Sucrose Iron Injection therapy for patients with anemia of pregnancy can significantly increased serum transferrin receptor levels,has higher safety and low adverse pregnancy outcomes rate.

Objective To observe the effects of metformin on the serum levels of adiponectin,interleukin-18(IL-18) and C-reactive protein(C-RP) in overweight/obese type 2 diabetes. Methods The changes of serum levels of HOMA2-IR,adiponectin,IL-18,C-RP and lipid profiles were observed before and after the treatment of metformin. Results After the eight weeks of treatment,the levels of adiponectin and IL-18 were not significantly changed,while C-RP(0.49±0.23 vs 0.89±0.22ng/dl),total cholesterol(TC)(4.58±0.83 vs 6.15±2.23mmol/L),LDL-C(2.65±0.78 vs 3.66±0.84mmol/L) and HOMA2-IR(1.1±0.7 vs 2.3±1.7) were decreased (all P<0.05),HDL-C(1.23±0.30 vs 1.02±0.24mmol/L) was increased (P<0.05). Conclusions Metformin may reduce the serum C-RP level and modulate the disorder of blood lipids,but may have no effect on adiponectin and IL-18

The properties of polyethyleneimine-cholesterol cationic lipopolymer (PEI-Chol) as gene carries and its gene transfer efficiency in vitro with lipid microbubbles were presented in this paper. PEI-Chol lipopolymer was synthesized by linking cholesterol chloroformate to the amino groups of branched poly(ethyleneimine) (PEI) of 1 800. The structure and molecular weight of PEI-Chol were confirmed by IR, 1H NMR and MADI-TOF-MS (matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry), respectively. The average molecular weight of PEI-Chol was approximately 2 000. The gene delivery system of bubble/PEI-Chol/DNA was constructed by mixed PEI-Chol/pDNA (N/P 10:1) complexes with lipid microbubbles (2-8 microm) which were prepared by DPPC, DSPE-PEG2000 and perfluoropropane with the reverse phase evaporation technique. pEGFP-Cl (enhanced green fluorescent protein) was used as report gene to investigate the DNA condensing ability of PEI-Chol lipopolymer by agarose gel electrophoresis. And their cytotoxicity and in vitro transfer efficiency of different complexes were compared with each other in A549 and MCF-7. The results indicated PEI-Chol lipopolymer can condense plasmid DNA when N/P ratio upto 4, PEI-Chol complexes and bubble/PEI-Chol/DNA complexes were nontoxic to A549 and MCF-7 when formulated at the N/P ratio of 10/1 as determined by MTT assay. This bubble/PEI-Chol/DNA delivery system provided good transfer efficiency with other desirable characteristics such as against-precipitation of plasma proteins. In conclusion, bubble/PEI-Chol/DNA complex is a novel non-viral gene delivery system.

Objective To analyze the genetic mutation patterns and the constituent ratio of α-thalassemia in Luzhou area and to investigate the clinical application value of the gene diagnosis .Methods The PCR method combined with DNA chip hybridization technique was adopted to conduct the gene detection and analysis on 116 cases of suspectedα-thalassemia .Results Among 116 sus-picious cases of α-thalassemia ,39 cases were found with genotypes of α-thalassemia ,the detection rate was 33 .62% .7 kinds of mu-tation genotypes were detected ,in which the deletion type of - -αSEA/ααaccounted for 41 .03% and the deletion type of -α3 .7/ααaccounted for 25 .64% .Conclusion The main gene mutation of α-thalassemia in Luzhou area is the deletion type of - -αSEA/αα. The gene diagnosis is an important criterion for definitely diagnosing α-thalassemia .

To explore the velocity difference between specifications and categories of liquid with the same pressure infusion bag in order to widen the specification range of the liquid for pressure infusion. Pressure infu-sion experiments were performed with three specifications of normal saline of 100, 250 and 500 ml to compare the veloci-ties of different specifications of liquid, and with 500 ml normal saline, (5%, 10% and 50%) glucose injection, 5% glu-cose and sodium chloride injection, 5% sodium bicarbonate injection, 10% fructose injection, 706 plasma substitute, 20%mannitol injection and etc to make clear the velocities of different categories of liquid. With the same pressure, there were no significant differences between the velocities of three specifications of liquid, and between those of cate-gories of liquid with the same specification and concentration; the difference was significant between the same category of liquid with different concentrations, and the velocity showed a negative correlation with the concentration. The pressure infusion bag is compatible with 100, 250 and 500 ml liquid, and the velocity may be constant in case some specification of liquid is replaced by another one. The velocity has to be regulated in case the concentration or category of the liquid changes, when the pressure infusion is performed.