Objective:To investigate the effect of salvia miltiorrhiza-derived Sal-miR-58 on the radiosensitivity of glioma U251 cells and its possible mechanism. Methods:Glioma U251 cells were treated with different concentrations of miR-Ctl or Sal-miR-58 mimic, and subsequently treated with radiation to establish the radiotherapy model in vitro. The effect of Sal-miR-58 upon U251 cell viability was assessed by MTT assay. The effect of Sal-miR-58 on apoptosis of glioma U251 cells was evaluated by Hoechst33342/ propidium iodide (PI) staining. The changes of reactive oxygen species (ROS) content in U251 cells were detected by 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe. The effect of Sal-miR-58 combined irradiation on the radiosensitivity of U251 cells was detected by clone formation assay. The expression levels of HCLS1-related protein X-1 (HAX1), apoptosis marker proteins B cell lymphoma 2 (Bcl-2), cleaved-cysteine-containing aspartate-specific protease (Cleaved-Caspase) 9 and Cleaved-Caspase 3 were detected by Western blot. Multi-group comparison was conducted by one-way ANOVA. Two-group comparison was performed by independent sample t-test. Results:Sal-miR-58 could exacerbate the inhibition of U251 cell proliferation after irradiation ( P<0.05). Sal-miR-58 could promote the apoptosis of U251 cells and increase the production of ROS in U251 cells after radiation ( P<0.05). Clone formation assay showed that Sal-miR-58 increased the radiosensitivity of U251 cells, with a radiosensitization ratio of 1.43. Western blot showed that Sal-miR-58 inhibited the expression of HAX1 in U251 cells. Sal-miR-58 could inhibit the expression of Bcl-2 and increase the activation of Caspase 3 and Caspase 9 by reducing the expression of HAX1. Conclusions:Sal-miR-58 enhances the radiosensitivity of U251 cells. The possible mechanism is that Sal-miR-58 inhibits the expression of HAX1 induced by radiation and accelerates the apoptosis process of tumor cells.

Objective: To investigate the interest and awareness of traditional Chinese medicine undergraduates on the course of Chinese medical literature, and to provide suggestions for further enhancement of undergraduates' cognition and in-depth study of this subject. Methods: Taking Shandong University of Traditional Chinese Medicine as an example, 726 undergraduates were sampled and randomly sampled by stratified sampling, and the interest and awareness of Chinese medical literature were suveyed and summarized, and the factors that led to this situation were analyzed. Results: A total of 119 (16.4%) knew and took the course, 78 (10.7%) said they often read Chinese medical literature on their own initiative, 455 (62.7%) said that the factors affecting their access to Chinese medical literature were reading difficulties. 410 (56.5%) students suggested that the course of Chinese medical literature should be open as an optional course. Conclusions The undergraduates have high interest and low awareness of the Chinese medical literature. It is suggested that the school should carry out curriculum reform and organize relevant publicity activities in order to increase the awareness of the undergraduates on the Chinese medical literature.