Objective To understand the current situation of smoking and the influencing factors among female students of a medical college in Chongqing .Methods By applying the stratified cluster random sampling method ,992 female students were sam-pled from 5 faculties 3 grades in this college for conducting the smoking questionnaire investigation .Results The smoking rate a-mong female students was 3 .51% and the attempting smoking rate was 9 .18% .Among them ,the higher smoking rate mainly fo-cused on the students from urban area ,which was 9 .30% .The female students without opposite attitude to college student′s smok-ing had the smoking rate of 12 .11% and the female students with peripheral friends smoking had the smoking rate of 28 .22% .The influencing factors of smoking among female students included the place where the students came from ,student′s attitudes to smok-ing ,the smoking status of their peripheral friends ,etc .Conclusion The smoking rate among female students in the medical college is basically consistent to that of the national level of female smoking ,but the total cigarette contact rate is higher .It is necessary to strengthen the frequent smoking control education and intervention on the female students in the medical colleges for effectively re-ducing the smoking rate and decreasing the smoking harm to the female health .

Objective To understand the current situation of smoking among students of a medical college in Chongqing and its influencing factors .Methods By applying the stratified cluster random sampling method ,1 437 students were sampled from 5 facul-ties 3 grades in this college for conducting the smoking questionnaire investigation .Results The smoking rate was 12 .69% ,which in males and females were 32 .59% and 3 .51% ,respectively .Among them ,the nursing faculty and the grade 1 had the lowest smok-ing rates ,which were 4 .02% and 8 .84% respectively ;the smoking rate in the students from different origins had no statistical difference(P>0 .05) .The influencing factors of smoking included male ,faculty ,fresh students ,parents with smoking habit ,drink-ing and the knowledge acquistion frequency of smoking harmful to health ,etc .Conclusion It is imperative to strengthen the fre-quent smoking control education and intervention among the students in the medical colleges ,especially to males and the fresh students ,for effectively reduce the smoking rate .

Objective To understand the smoking intention in future 3-5 years among the students of a medical college in Chongqing .Methods According to the stratified cluster random sampling ,1 457 students from 3 grades in 5 faculties were sampled and performed the questionnaire investigation on the smoking behavior and smoking intention .Results The smoking rate was 12 .69% and the attempting smoking rate was 14 .95% .Among them ,the smoking rate and the attempting smoking rate in males were 32 .59% and 27 .46% ,and which in females were 3 .51% and 9 .18% ,respectively .The proportions of the students with hesi-tation and affirmation for answering the smoking intention in future 3-5 years and the current smoking students were 49 .44% and 23 .89% respectively ;which were 34 .82% and 14 .29% in males and 10 .52% and 1 .65% in females respectively ;which were 10 .24% and 3 .01% in grade 1 ,24 .73% and 7 .44% in grade 2 and 20 .30% and 6 .70% in grade 3 .Conclusion Partial medical students still insist on smoking in future ,and especially the students with the hesitating attitude of smoking in the future occupy a higher proportion .So it is necessary to strengthen the smoking control work in medical college and the antismoking attitude educa-tion .

Objective To gain purified platelets for further research of the immune function of platelet.Methods Platelets were purified by positive(MS) and negative(LD) magnetic cell sorting(MACS) separation.The percentage of activated platelets was detected by flow cytometry and nucleated cell clearance was evaluated by white cell count.Results The percentage of activated platelets before separation was(2.39?1.10)%,and increased to(2.56?1.08)% and(16.76?4.04)%,respectively,after MS and LD MACS.The clearances of nucleated cells after MACS MS and LD were(98.44?0.24)% and(98.47?0.18)%,respectively.The recovery rate of purified platelet after MS and LD was(76.50?1.49)% and(79.20?2.61)%.Conclusion MACS LD method was more suitable for the platelet purification.

Objective To investigate the effects of rosiglitazone on proangiogenesis function of human umbilical vein endothelial cells ( HUVEC) in high glucose environment. Methods HUVECs were cultured in high glucose environment and stimulated by rosiglitazone. MTT, cell scratch test and Transwell assay were used to detect HUVEC proliferation and migration. The concentration of VEGF, SDF-1 was also detected in the supernatant. Results Rosiglitazone could effectively promote HUVEC proliferation and migration. The concentration of VEGF and SDF-1 in rosiglitazone stimulated supernatant was higher than that in high glucose group. The inhibition of AKT signal could block the promotion of rosiglitazone on the HUVEC proliferation, migration and secretion. Conclusion Rosiglitazone could significantly promote HUVEC secretion, proliferation and migration in high glucose environment. AKT signal played an important role in this process.

Objective:To study the specific photochemical effects of a newly designed target photosensitizer. Methods Based on the technique of antisense nucleic acid and the principle of photochemical reaction effects,a specific sensitizer,TFO P has been designed and synthesized.When in coordination with long wave ultraviolet ray(UVA) ,this decorated complex (TFO P) was added into the blood cell suspension to inactivate the contaminating virus( duck hepatitis virus B,DHBV).The efficacy of specific binding to DHBV DNA and viral inactivation by TFO P was detected by gel shift blot assay and infection of primary culture of duck hepatocyte.Results The designed TFO P could specifically bind to different DHBV DNA line sample and present different linking level.With a TFO P concentration of 0.1 nmol/ml and UVA intensity of 1800 ?W/cm 2,the DHBV in blood cell suspension could be reduced by 1.90～5.40 logs.Conclcusion The photochemical effects of TFO P could significant inactivate DHBV in blood.

Objective:To investigate the soldiers' depression after sea field training and influence factors.Methods:1107 navy men were evaluated by Zung's Self-Rating Depression Scale (SDS) and Eysenck personality questionnaire (EPQ) after field training.Results: The rate of depression in the study group was 25.04%, N score of the soldiers after sea field training was significantly higher than the norm of Chinese army men. Logistic regression analysis revealed that the factors of nervousness, dyscoimesis, medical history during sea field training, restless, introversion, the level of education, living environment before joining in the army, hunger and so on had influenced the level of depression.Conclusion:The total incidence of depression in soldiers after sea field training is higher than the norm of army men. It is associated with stress, personality characteristics, training factors, general malaise, psychological pressure and the level of education.

Objective Study on fetomaternal immuno state and RHD type of a pregnant woman of weak D phenotype.MethodsThrough polymerase chain reaction (PCR)、direct genomic DNA sequencing and flow cytometry.ResultsIn both sequence specific promer (SSP) PCR and the sequencing PCR tests, the sample was detected negative in exons 3 6 of the RHD gene, whereas all other exons (exons 1 2,7 10) were tested positive. And the sequence of detected exons (exons 1 2,7 10) are the same with normal RHD in GenBank (accession no. AJ299020 1 and AJ299026 9). Serologically and genetically, the sample can be designated as D category VI type Ⅲ. Through a duce tube PCR method, the RhD zygosity of this individual was typed CD VI e/cde。In flow cytometry, a few fetal erythrocytes were detected in peripheral blood of the mother. However there were no anti D detected in sera and hemolytic disease of the newborn(HDN) observed at all.ConclusionSevere cases of HDN have occurred in D positive babies born to partial D mother with anti D, although HDN don't take place in this case. We may still consider D VI phenotype individuals as D positive donors and D negative receiptions in our transfusion practice and in clinical anti D allo immune prophylaxis and monitoring.

Objective　To explore the effect of photon on blood biologic components in blood photochemical treatment. Methods　After the blood sample was adjusted to an appropriate density, it was treated with 0.1 nmol/ml 8-MOP (8-methoxypsoralen), 0.1 nmol/ml TFO (triple helix-forming oligonuletide) and UVA (ultraviolet A radiation) at the intensity of 1 800 μW/cm2 for 3～20 min. The changes of biologic activities of major components in blood were measured with automatic blood gas analyzer, platelet aggregation analyzer, blood coagulation analyzer, micropipette aspiration system and assay of poly-lysine adsorption. Results　The oxygen content in blood was increased gradually. The resilience of erythrocyte was enhanced ，but its adhesiveness was decreased. The parameters related to blood coagulation had some changes but all remained within the normal ranges. Conclusion　Under the definite condition of blood virus being inactivated effectively, the nonspecific effect of photosensitive response may improve blood oxygen content, enhance the transfiguring ability of erythrocyte and decrease the blood viscosity, but having no obvious change on blood coagulation.

BACKGROUND:At present,new drug and dosage form to treat diabetes mellitus has emerged continually,but it has toxic reaction,side effects,and contraindication or unindication.Especially that it cannot prevent the natural proceeding of diabetes mellitus from aggravation.Based on theory and experience of traditional Chinese medicine,and combining modern methods of pharmacology and pharmaceutical chemistry,it has become one of the hotspots in researches on diabetes mellitus at present to investigate and empolder preparation of pure traditional Chinese medicine that has good effect and little toxicity.OBJECTIVE:To investigate the pharmacological effect of Chinese compound of Qiangyi Jiangtang capsules on diabetic rat models induced by high-fat forage and streptozotocin.DESIGN:A comparative observation of animal trial.SETTING:Staff Room of Pharmacology,Yunnan College of Traditional Chinese Medicine.MATERIALS:Totally 100 2-month-old Wistar rats of common degree,weighing(180±25)g were used.There were 50 males and 50 females,14 rats were randomly selected as the blank control group,and the other 84 rats were used in model establishment.The treatments of the animals in the experiment were accorded with the ethical standard.METHODS:The experiment was carried out in the Staff Room of Pharmacology,Yunnan College of Traditional Chinese Medicine from March to April in 2004.Diabetic models were induced by high-fat forage and streptozotocin.The successful model rats (n=54) were randomly divided into the model group,positive control group and the high,middle and low dosage of Qiangyi Jiangtang capsules groups.The dosages in the low,middle and high dosages treated groups were 0.4,1.2 and 2.0 g/kg respectively. The rats in the blank control group and model group were given isovolume of saline,and those in the positive control group were administrated intragastrically with metformin (0.5 g/kg).After administration,blood glucose,glycosylated hemoglobin and serum fructosamine were determined at 1 month,and the amounts of water drinking and food intake were determined at the 2nd and 4th weeks.MAIN OUTCOME MEASURES:Results of blood glucose,glycosylated hemoglobin and serum fructosamine;amounts of water drinking and food intake.RESULTS:Totally 100 Wistar rats were used,32 of them were excluded due to failure in the model establishment,and the other 68 were involved in the final analysis of results.The blood glucose,glycosylated hemoglobin and serum fructosamine in the high,middle and low dosages of Qiangyi Jiangtang capsules groups were all significantly lower than those in the model group(P＜0.01).The amounts of water drinking at the 2nd and 4th weeks in the high,middle and low dosages of Qiangyi Jiangtang capsules groups were significantly lower than those in the model group (P＜0.01),and the amounts of food intake were also lower than those in the model group (P＜0.05 or 0.01).CONCLUSION: Qiangyi Jiangtang capsules could remarkably decrease the levels of blood glucose, glycosylated hemoglobin and serum fructosamine,and the amounts of water drinking and food intake of rat models of diabetes mellitus.It was proved that traditional Chinese medicine for diabetes mellitus is worthy of further development and research.