Objective To observe the therapeutic efficacy of combination of smecta and P.O.Bifidobigen in treatment of infantile diarrhea .Methods 100 cases with infantile diarrhea were collected randomly. 6 2cases were treated with combination of smecta and P.O.Bifi dobige n,while 62 cases were treated with s mecta alone .Results 62 cases(62%)showed an obvious efficacy ;30 cases(30%)did effective;8cases(8%)showed no effective .The total effective rate was 92.4%.For infectious and symptomatic diarrhea,there was not difference of effective rates between the two therapies.For digestive dysfunction and chronic diarrhea accomplished with malnutrition,just the combination of the two drugs showed efficacy .Conclusions For infantile diarrhea,the combination of smecta and P.O. Bifidobigen can result in efficacy in addition to controlling its causes.

Objective To explore the effect of antibiotics to neonatal intestinal microflora.Methods the neonatus who had infectious pneumonia were selected as case-groups,and divided into three sub-groups according to the antibiotics the patient used randomly.Tak- ing the period when all indexes were normal as pneumonia being cured,and curing rate and time-effect were compared among groups.To explore the effect of antibiotics to neonatal,the main microflora which can reflect the all intestinal microflora were quantitatively analysed when antibiotics had been used three and seven days respectively.Results The results showed that:①there is no significant difference on curing rate and time-effect among the three groups;②antibiotics showed effect to neonatal intestinal microflora,and the longer the antibi- otics was used,the more significant the effect was;③the group with penicillin and cynnematin simultaneously showed the most effect to in- testinal microflora,and the group with cynnematin only showed moderate effects on intestinal microflora,and the group with penicillin showed little effect to intestinal microflora.

Objective To explore the blood sugar controlling for elderly patients with fracture and diabetes mellitus. Methods Twenty patients with fracture complicated with diabetes were managed with mental education and diet controlling. Meanwhile nursing measures were done to prevent infections, bedsores and deep vein thrombosis (DVT). Results The fasting blood sugar levels of the 20 elderly patients were well controlled below 7.8 mmol / L. There were no related complications. Conclusion Such nursing measures as psychological counseling and diet control are helpful for a steady maintenance of blood sugar for feasibility of surgical operation, to ensure successful manipulation of the surgical operation. Prevention of infection, bedsore and DVT is critical for ensuring the success of operations.

Objective To discuss the major cardiovascular disease(CVD) risk factors and volume load of women.Methods A cross-sectional study was conducted on adult women who came to our hospital for health examination.The clinical data included age, past medical history, smoking history, drinking history, body measurements (height, body weight, blood pressure, etc.), and blood serum creatinine, hemoglobin, blood glucose, blood lipid and other biochemical indicators were detected.Using the human body composition analyzer, the extracellular fluid (ECW), intracellular fluid (ICW), total water(TBW), and ECW/TBW ratio (E/T) were used to evaluate the capacity of the cells.Total body water(TBWwatson) was Calculated according to Watson formula.The clustering of four major CVD risk factors (defined as two or more of the following: hypertension, diabetes, dyslipidemia and overweight) and their association with volume load were investigated.Volume load was evaluated by bioelectrical impedance analysis.Hypovolaemia was defined as extracellular water/total body water (ECW/TBW) at and under the 10 percentile for the normal population.Logistic regression model was use to explore the relationship between capacity status and risk factors of CVD.Results A total of 2 433 women (mean age (37.4±7.3) years old) were enrolled in the study, 14.7％ of them had clustering of CVD risk factors.Hypovolaemia in clustering group was statistically higher than either in the single or in the none risk factor group, which was 18.2％, 11.9％, 6.7％, respectively (x2 =45.4, P＜0.001).After adjusted for potential confounders (including age, hemoglobin, serum uric acid, estimating glomerular filtration rate (eGFR), hypovolaemia was associated with clustering of CVD risk factors, with an OR of 2.76 (95％ CI 1.93-3.95).Conclusion Hypovolaemia is associated with clustering of major CVD risk factors in women, which further confirms the importance of modifying lifestyle to reduce the burden of CVD.

Objective To investigate the influence of high-fat diet on blood pressure and metabolism in Sprague-Dawley(SD) rats and its mechanism.Methods Forty male SD rats were randomly divided into 4 groups which were fed with a diet containing 53% calorie as fat(HF) or a normal diet(ND) for 5 or 10 weeks.Systolic blood pressure(SBP),body weight,abdominal adipose tissue,blood lipids,fasting insulin(FINS),and fasting plasma glucose(FPG) were measured after 5 and 10 weeks respectively.Results SBP of HF groups were higher than that of ND groups [HF5 vs.ND5,(105.506?4.634)mmHg vs.(100.060?4.773)mmHg,P

Objective To evaluate the reliability and sensitivity of detection of thyroid carcinoma lymph node micrometastasis with MUC1.Methods In 488 cases of thyroid disease,1% methylene blue 1.0-2.0mL was injected into the thyroid nodule or surrounding thyroid tissue 24hours before operation.The dye-stained lymph nodes were detected at operation and RT-PCR method was used to determine MCUI in the clinically blue-stained lymph nodes.Results The detection rate of the blue-stained lymph nodes in thyroid carcinoma was 93%,and in benign lesions was 0%.In the research group,80 cases were found to have blue-stained lymph nodes,the detection rate was 95% and was higher than the pathologr diagnostic rate which was 86%(P

Objective To evaluate the risk factors for intracranial hemorrhage in premature infants. Methods Cochrane Library, PubMed, ScienceDirect, Chinese Academic Literature Main Database, Chinese Science and Technology Periodi-cal Database, Wanfang Periodicals and Dissertation Database were searched for articles published from January 2000 to December 2012 on the risk factors of intracranial hemorrhage in premature infants, with retrospective retrieval and manual retrieval as supplement. RavMan5.2 provided by Cochrane was used for meta-analysis. Fixed-or random-effects models were selected according to the results of heterogeneity test. Results Nine studies were conifrmed to be eligible. Odds ratio (OR) and 95%conifdence interval (CI) of the risk factors were as follows:gestation age≤32 weeks (OR=3.29, 95%CI=2.76-3.91), birth weight≤1 500g (OR=2.68, 95% CI=2.24-3.20), maternal complications (OR=1.59, 95% CI=1.23-2.06), intrauterine distress or birth asphyxia (OR=2.42, 95% CI=2.06-2.84), mechanical ventilation (OR=3.23, 95% CI=2.55-4.09), metabolic acidosis (OR=2.88, 95%CI=2.04-4.05), use of high concentration of oxygen (OR=2.98, 95%CI=1.63-5.44), prenatal use of dexametha-sone (OR=0.69, 95%CI=0.55-0.86), respiratory distress syndrome (OR=1.57, 95%CI=1.04-2.39). Those differences were all statistically signiifcant. There was no difference in caesarean section (OR=0.99, 95%CI=0.84-1.17), multiparity (OR=1.05, 95%CI=0.79-1.40) and gender (OR=1.25, 95%CI=0.97-1.59). Conclusions The risk factors for intracranial hemorrhage in premature infants are gestation age≤32 weeks, birth weight≤1 500 g, maternal complications, intrauterine distress or birth asphyxia, mechanical ventilation, metabolic acidosis, use of high concentration of oxygen, respiratory distress syndrome, but prenatal use of dexamethasone can reduce the incidence of intracranial hemorrhage in premature infants.

Objective:This study aimed to investigate the relationship between estrogen receptor (ER) and Bcl-2 in cervical can-cer and precancerous lesions. Methods:ER and Bcl-2 were evaluated by immunohistochemistry in 20 non-tumor tissues, 30 low-grade cervical intraepithelial neoplasias, 70 high-grade cervical intraepithelial neoplasias, and 80 cervical cancers. Results:The positive rate of ER was 75%in non-tumor tissues;this rate decreased with disease severity (χ2=24.266, P<0.01). The overexpression of ER protein was remarkably lower in cervical intraepithelial neoplasias (CIN) Ⅱ-Ⅲ and carcinomas than in non-tumorous cervices. Differences were not significant between CINⅠand non-tumorous cervices (χ2=0.751, P=0.386). Furthermore, ER expression was not correlated with histological type and tumor grade. The positive rate of Bcl-2 was 75.38%in squamous cell carcinoma. This rate increased with dis-ease severity (χ2=27.715, P<0.01). The Bcl-2 protein overexpression was remarkably higher in CINⅠ, CINⅡ-Ⅲ, and carcinomas than in non-tumorous cervices. Bcl-2 expression was also not correlated with histological type (P=0.500);by comparison, Bcl-2 expression was correlated with tumor pathological grade (χ2=4.862, P=0.027). The co-expression of ER and Bcl-2 was 38.57%and 15.38%in CINⅡ-Ⅲand squamous cell carcinoma, respectively;the differences between these two values were significant (χ2=9.108, P=0.003). A cor-relation between ER and Bcl-2 was observed in CINⅡ-Ⅲ(r=0.506, P<0.01);no correlation was observed between other groups. Con-clusion: ER expression was inversely correlated with Bcl-2 in cervical cancer progression. The co-expression of these markers may have an important function in the course of high-grade cervical intraepithelial neoplasia.

Objective To study the correlation of p53,ki-67 and bcl-2 expression in breast cancer with the clinical response to neoadjuvant chemotherapy,and search for biological markers to guide therapy and predict prognosis.Methods The expression of the p53,ki67 and bcl-2 was examined by immunohistochemical(assay) in samples of breast cancer tissue which taken from 118 cases of operable breast cancer patients.The relationship between p53,ki-67 and bcl-2 and the effect of neoadjuvant chemotherapy was analyzed by(chi-square) test.Results The overall response rate to neoadjuvant chemotherapy was 68.6%.Patients with expression of ki-67 were more likely to respond to treatment.The effect of neoadjuvant chemotherapy had a significantly negative correlation with p53 expression.There was no significant difference between the response to neoadjuvant chemotherapy and bcl-2 expression.Expression of p53 protein was correlated with low(expression) of bet-2.Expression of p53 and ki-67 protein was significantly related.Conclusions The(results) indicated that p53 and Ki-67 expressions are strong prognostic molecular markers that can be a guide for neoadjuvant chemotherapy and prediction of prognosis for patients with breast cancer.

ObjectiveTo investigate the application effect of Delphi method to establish the preoperative visit mode. MethodsTaking the Delphi method to establish the standardized procedures of preoperative visit.The average length of stay,hospital costs and satisfaction degree of patients before and after application of standardized procedures of preoperative visit were compared. ResultsThe study showed that after taking the Delphi method to establish the preoperative visit mode,the patients' hospital stay for the same type of surgery,treatment costs and satisfaction degree of patients improved significantly than those before.The differences before and after treatment were significant. ConclusionsUsing Delphi method to establish a standardized procedures of preoperative visit has the advantage of strong target,strong representation,high degree of authority.It takes advantage of the excellent medical care experience and provides uniform and stable preoperative preparation work.It lays a foundation for patients to get better medical care and is worthy of clinical application.