Objective To estimate quantitatively the relationship between female lung cancer and cooking oil fume (COF)?Methods Meta-analysis was employed to estimate the relationship between COF and non-smoking female lung cancer based on8relevant epidemiological articles publisked at home and abroad?Fixed Effect Model Analysis was used to calculate the incorporated odds ratio(OR c )based on homogeneity test.Results The OR c for female lung cancer associated with COF was2.94(95%CI =2.43～3.56)with statistical significance(? 2 =120.64,P

Objective To study the effect of Gadd genes expression in surveillance of DNA damage. Methods The human liver tumor cells were treated with alkylating agent(nitrogen mustard) and total RNA was extracted, the expression levels of Gadd genes were determined by RT-PCR. Results Treated with 1 ng/?l nitrogen mustard for 3 h, 24 h, 48 h respectively and with 1 ng/?l, 5 ng/?l nitrogen mustard for 24 h respectively, the expression levels of Gadd genes increased at first (P0.05). Conclusion Gadd genes expression can be used as the indexes in the surveillance of DNA damage induced by nitrogen mustard.

Objective To investigate the effects of different hemostatic methods in laparoscopic ovarian cystectomy on ovary functions . Methods A total of 90 cases of laparoscopic ovarian cyst resection from June 2010 to June 2013 were selected .They were divided into bipolar electrocoagulation group , monopolar electrocoagulation group , and suture group according to the doctor ’ s favor.The FSH, LH, and E2 before and after surgery were compared among the three groups . Results All patients were followed up for more than 1 year.The rate of patients with menstrual disorder in monopolar electrocoagulation group [33.3%(10/30)] was significantly higher than that in bipolar coagulation group [10.0%(3/30),χ2 =4.812,P=0.028] and suture group [6.7%(2/30),χ2 =6.667,P=0.010].There was no significant difference between bipolar premature coagulation group and suture group (χ2 =0.000, P=1.000).There was no significant difference in FSH ,LH,and E2 among the three groups before and 1 month after surgery (P>0.05).There was no significant difference in FSH ,LH, E2 between bipolar premature coagulation group and suture group 6 months after surgery (P>0.05).In the monopolar electrocoagulation group , the FSH [(10.43 ±2.04) U/L] and LH [(18.58 ± 3.82) U/L] levels were significantly higher than those in the bipolar electrocoagulation group [(8.12 ±1.82) U/L and (13.31 ± 2.53) U/L, P<0.05], and in the suture group [(7.08 ±1.68) U/L and (12.61 ±2.68) U/L, P<0.05].The E2 level in the monopolar electrocoagulation group [(252.5 ±26.9) pmol/L] was significantly lower than that in the bipolar electrocoagulation group [(321.3 ±28.2) pmol/L, P<0.05] and in the suture group [(313.7 ±31.4) pmol/L, P<0.05]. Conclusion In laparoscopic ovarian cystectomy , bipolar coagulation hemostasis is safer than monopolar electrocoagulation hemostasis , being worthy of clinical application .

Objective To analyze the distribution and content characteristics of highly cited pa pers in Chinese nursing journals,in order to give a reference for nursing researchers to design their studies and to improve more high quality nursing papers.Methods Nursing papers were searched from database of China Academic Journal Network.Papers cited 100 times or more were collected for statistical analysis.Results There were 266 nursing papers cited 100 times or more.They were found in 9 nursing journals,of which Chinese Nursing Journal was 69.1％.The paper most highly cited was 1 298 times.The contents of highly cited papers covered basic nursing (infusion nursing and air way nursing),health education,nursing management (safety management,nursing laws,and nursing profession protection),humanistic care,psychological nursing care,and pressure of nurses.Conclusions In order to produce high quality of nursing paper,nursing researchers should improve their academic sensitivity,pay more attention to the scientific fronts,emphasize basic nursing research and study persistent.

Objective To evaluate reliability and validity the Chinese-version of chronic pain acceptance questionnaire-8(CPAQ-8).Methods The reliability and validity of CPAQ-8 was tested in 223 old patients with chronic pain.Result The Chinese-version of CPAQ-8 was (23.79±-5.23),AE was (12.84±4.96),PW was(10.97 ±2.90).Cronbach' s of CPAQ-8 was 0.853,and Guttmann Split-Half Coefficient was 0.843.Two factors were extracted by factor analysis through exploratory factor analysis,whose cumulative contribution was 58.217％.While confirmatory factor analysis showed probability level of the modified model was P=0.322,with indexes as follows: x2/df =1.124,GFI =0.979,AGFI =0,956,TLI =0.069,RMSEA =0.024,NFI =0.960,TLI =0.992,CFI =0.995.Conclusion Reliability and validity of CPAQ-8 were both well.

Objective To investigate the correlations of ATP-binding cassette transporter A1 (ABCA1) protein expression in renal interstitium with foam cells formation,tubulointerstitial lesion and serum cholesterol in glomerulonephropathy.Methods Five patients with Alport syndrome,28 patients with membranous proliferative glomerular nephritis (MPGN),35 patients with focal segmental glomerulosclerosis (FSGS),36 patients with idiopathic membranous nephropathy (IMN) and 34 patients with IgA nephropathy (IgAN) were enrolled in the study.Expression of ABCA1 protein was detected by immunohistochemical method.The relative area of foam cells in renal interstitium was semi-quantified and the correlations of ABCA1 protein expression in renal interstitium (except for renal tubules) with the relative area of foam cells,integration of tubulointerstitial lesion (TIL) and serum cholesterol were examined using t test and linear correlation analysis.Results In renal interstitium,monocyte-macrophages,foam cells and renal tubular epithelial cells expressed ABCA1 protein.ABCA1 protein expression in renal interstitium with foam cells infiltration was significantly higher as compared to that without foam cells infiltration (P＜ 0.05),but there was no correlation between ABCA1 expression and foam cells area (P＞0.05).In MPGN,FSGS,IMN patients,there was a positive correlation between ABCA1 protein expression in renal interstitium and integration of TIL (P＜0.05).There was a positive correlation between serum cholesterol level and ABCA1 protein expression in renal interstitium (P＜0.05).Conclusions In glomerulonephropathy,cholesterol may promote the ABCA1 expression in renal interstitium.ABCA1 may have double-effects on transportation of cholesterol.ABCA1 may take part in the process of tubulointerstitial lesion.

AIM: To study the regulatory effect of curcumin on expression of heme oxygenase-1 (HO-1) in the lung of rat treated with LPS. METHODS: Eighteen rats were divided into three groups injected with different agents via lingua vein: control group (animals received equivalent saline) , LPS group (animals received a bolus dose of LPS 5 mg?0.5 mL-1?kg-1) and LPS+ curcumin group (animals received AP- 1 inhibitor curcumin 20 mg?0.5 mL-1?kg-120 min before the injection of LPS 5 mg ?0.5 mL-1?kg-1) . The expression of HO-1 mRNA and protein in the lung were examined 7 h after LPS administration by reverse transcribed polymerase chain reaction (RT- PCR) and Western blotting, respectively. Carboxyhemoglobin (HbCO) formation within pulmonary tissue was measured to represent CO content. RESULTS: The results showed that HO- 1 mRNA and protein expression as well as CO content in the lung of rats in LPS group were significantly higher than those in control group (P

AIM: To study the role of carbon monoxide (CO) in the mechanism of cholecystokinin-octapeptide (CCK-8) for attenuation of acute lung injury (ALI) induced by lipopolysaccharide (LPS). METHODS: Fifty-six adult male rats were randomly divided into seven groups: control group, LPS group, LPS+ZnPP (a specific inhibitor of HO-1) group, LPS+Hemin (Hm, CO donor) group, CCK-8+LPS group, CCK-8+LPS+ZnPP group and CCK-8 group (n=8 for each). Bronchoalveolar lavage was performed 2 h, 6 h and 12 h respectively after treatments. The numbers of polymorphonuclear leukocytes (PMN) in bronchoalveolar lavage fluid (BALF) was detected. The mortality of rats and the structure of lung tissues were observed. MDA and CO contents in lung tissues were also measured. RESULTS: The mortalities of rats were both zero 2 h and 6 h after agent administration. The mortality of rats was higher than control group 12 h after LPS administration. The mortality of rats in LPS+Hm and CCK-8+LPS group were lower than that in LPS group, and its in LPS+ZnPP and CCK-8+LPS+ZnPP group were lower than that in LPS and CCK-8+LPS group, respectively. Lung injury was observed in LPS group. At the same time the number of PMN, MDA and CO content were higher than those in control group. The degree of lung injury, PMN numbers and MDA content were lower, while CO content in LPS+Hm and CCK-8+LPS group were higher than those in LPS group. However, the degree of lung injury, PMN number and MDA content were higher, CO content were lower in LPS+ZnPP and CCK-8+LPS+ZnPP group than those in LPS and CCK-8+LPS group, respectively. CONCLUSION: CCK-8 attenuates the LPS-induced acute lunginjury by means of anti-oxidation and inhibition of PMN aggregation, which are both mediated by CO.

Objective To investigate the effects and mechanism of zinc on airway inflammation in asthmatic rats.Methods Animal model of asthma was established by OVA challenging,zinc-deficient and zinc-supplementary diet were given accordingly.Thirty-two SD rats were divided into four groups according to weight: zinc-deficient diet with OVA challenge group(group A),zinc-supplementary diet with OVA challenge group(group B),zinc-normal diet with OVA challenge group(group C) and zinc-normal diet with saline challenge group(group D).Twenty-four hours later asthma was induced,right side lungs were harvested for HE staining to observe histomorphological changes and the respective numbers of eosinophirs,neutrophils and macrophage in the bronchial mucus were counted.The contents of interferon-?(IFN-?),interleukin-4(IL-4),superoxide dismutase(SOD),malondialdehyde(MDA) and glutathione peroxidase(GPX) in lung homogenate were detected.Results Compared with group C,the number of eosinophils,neutrophils and macrophage,and the contents of SOD increased in group A,but significantly decreased in group B(P

Purpose To observe the pathologic types of glomerular diseases which have high renal interstitial foam cells infiltration and to evaluate the relationship between infiltration of foam cells (FC) in renal interstitial tissue and pathologic parameters.Methods A total of 2 862 patients who had received renal biopsy were enrolled in this study. Patients with Alport syndrome (AS,n=5), membranous proliferative glomerular nephritis (MPGN,n=28), focal segmental glomerulosclerosis (FSGS,n=144), idiopathic membranous nephropathy (IMN,n=132), IgA nephropathy (IgAN,n=893) were divided into two groups:FC+ group with foam cells and FC- group without foam cells.Results Infiltration of foam cells in renal interstitial tissue was commonly seen in AS.The frequency of interstitial foam cells was 46.43% in MPGN, 20.14% in FSGS, 13.64% in IMN, and 6.27% in IgAN. It was found that the segmental glomerular sclerosis and interstitial fibrosis were more severe in FC+ group than that in FC- group.Conclusions The renal interstitial foam cells are most common in patients with AS, but also seen in patients with MPGN, FSGS, IMN and IgAN. There might be a relationship between glomerular sclerosis, interstitial fibrosis and infiltration of foam cells. The present of foam cells in the renal interstitial tissue may contribute to the progression of renal diseases.