WIF-1 is an important antagonist in the Wnt/?-catenin pathway.As a tumor suppressor, WIF-1 is down-regulated in majority cancers but when it is correlated with WIF-1 promoter hypermethylation and treated with demethylation drug, its expression can be restored.Functional loss of WIF-1 due to promoter hypermethylation plays an important role in a tumor's pathogenesis, possibly mainly through aberrant canonical Wnt/?-catenin signal activation.The correlation of the expression of WIF-1 with clinicopathological features in different cancers is not identical.Detecting WIF-1 and promoter hypermethylation may offer help for early diagnosis, therapy and predicting prognoses.

PGD_2 and its signal transduction way play a critical role in the pathogenesis of bronchial asthma, such as immune and allergic inflammatory responses.So more and more investigators pay attention to it.This paper reviews that PGD_2 and its recepors effect on the pathologic changes in asthma.

Objective To investigate the efficiency ofKangfuxing liquid, ethacridine lactate in combination with local oxygen therapy for radiation-induced skin damage.Methods A total of 96 patients affected by radiation-induced skin damage were enrolled and randomly allocated to a treatment group and a control group by random number table method, with 48 patients in each group. All patients in the control group were treated with trolamine cream, and those in the treatment group were treated withKangfuxing liquid, ethacridine lactate in combination with local oxygen therapy. Skin dermatitis was scored utilizing Radiation Therapy Oncology Group (RTOG) toxicity scale.Results The total effective rate in the treatment group was significantly higher than that in the control group (97.9%vs.79.1%;χ2=10.119,P=0.002). The distribution of patients with RTOG grades 0 to 4 showed different degrees of improvement, and the number of patients with RTOG 0-4 grades changed from 0, 3, 9, 12, 24 before the treatment to 8, 12, 22, 6, 0 after the treatment in the treatment group, 0, 2, 10, 25, 11 to 2, 5, 18, 23, 0 in the control group. The percent of patients with RTOG grade 0 in the treatment group was significantly increased than that in the control group (16.7%vs. 4.2%;χ2=4.019, P=0.045) and the percent of patients with RTOG grade 3 significantly decreased (12.5%vs.47.9%;χ2=12.649, P<0.001). The percents of patients with dispirited spirit (31.3%vs. 58.3%;χ2=6.066,P=0.014), dry and chapped skin (20.8%vs. 58.3%;χ2=12.588,P=0.000) in the treatment group were all significantly lower than those in the control group.ConclusionKangfuxing Liquid, ethacridine lactate in combination with local oxygen therapy can improve radiation-induced skin damage.

Objective To investigate the effect of electroacupuncture plus early rehabilitation training on the function recovery after surgery for cervical spinal cord injury. Methods Sixty patients with cervical spinal cord injury undergone anterior cervical decompression and internal fixation were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by electroacupuncture plus rehabilitation training, while the control group was by rehabilitation training alone. The Frankel’s grading (FG), Barthel Index (BI), and Functional Independence Measure (FIM) were adopted for evaluation before and after intervention. Results The FG was not significantly changed after intervention in both groups (P>0.05). After intervention, there was no significant difference in comparing FG between the two groups (P>0.05). The BI and FIM scores were significantly changed after intervention in both groups (P＜0.01). There were significant differences in comparing BI and FIM scores between the two groups (P＜0.05). Conclusion Electroacupuncture plus early rehabilitation training can promote the function recovery after surgery for cervical spinal cord injury, and improve the quality of life.

Circulating tumor cells (CTCs) in breast cancer patients can be detected by Cell Search system. Results from recent studies suggested that CTCs level might serve as a prognostic marker and be used in the early assessment of therapeutic response in patients with metastatic breast cancer. However the role of CTCs in early stage breast cancer is not well established. Large prospective trials are needed to further understand its biological characteristics and to confirm its role as a predictive and prognostic marker.

Objective To know the sleeping quality of clinical nurses in Urumqi, and analyze the influencing factors, put forward the corresponding countermeasures for different factors to improves the sleeping quality. Methods Randomly selected 407 nurses in clinical departments as the research objects in Urumqi top three hospitals, using the Pittsburgh sleep quality index scale (PSQI) and general situation questionnaire to investigate, and then analyzed the results of the survey. Results The incidence rate of sleeping disorders in emergency nurses was the highest (80.00%), followed by pediatric, intensive department, internal medicine, surgical department, operating room, outpatient and others was the lowest (39.02%). Gender (t=-2.015, P =0.045), age (F=8.509, P =0.000), establishment(F=3.609, P =0.013) were the influence factors of PSQI scores. Only the difference of the scores of hypnotic drugs between different ethnic groups was significant, P<0.05, while the others had no significant difference, P>0.05. Conclusions Poor sleeping quality of nurses existed in every department, its sleeping quality status, department, gender, age and establishment have certain correlation, interventions should be strengthened, to improve the overall level of sleeping.

AIM:The model of Salvia miltiorrhiza extracted by alcohol based on adaptive network-based fuzzy inference system(ANFIS)was studied in this paper.METHODS:The ANFIS model was set up by a set of experimental data which were gained on a pilot extraction condition.Then the tanshinone ⅡA content of the alcohol extract from Salvia miltiorrhiza in processing conditions could be predicted by this ANFIS model.The Radix Salvia miltiorrhiza was extracted by alcohol thermal reflux;then initially created a fuzzy inference system model based on network and trained by mixing learning arithmetic.RESULTS:The relative errors between the predicted values and the experimental values were in a allowable range,there were only 4 points greater than 10% of totally 90 calibration points.CONCLUSION:The analytical and experimental results show that the method can effectively forecast the content of the effective ingredient according to preset extraction conditions.

Objective To investigate the expression of tumor suppressor gene PTEN in breast carcinomas and its significance. Methods Immunohistochemical method was used to detect the expression of PTEN gene in 146 cases of breast carcinoma and 10 specimens of normal breast tissue closely adjacent to carcinoma. Results It was showed that PTEN gene was expressed in all 10 specimens of breast tissue closely adjacent to carcinoma. Expression of PTEN was localized in cytoplasm and nuclei of epithelial cells of lobular acini and epithelial cells of ducts. The rate of PTEN expression was 57.5% (84/146) in breast carcinoma. Expression of PTEN was related to tumor size, pathological stage, and the expression of ER and PR of breast cancer. The 2-year disease free survival of PTEN high expression breast cancer patients was superior to those with low expression (P

Background The impact of statins on inflammation are independent of cholesterol-lowering effect.Recent studies showed that Toll-like receptor 4(TLR4),a mediator of innate immune responses,is involved in the initiation and progression of atherosclerosis.Objective To investigate the effects of atorvastatin on LPS-induced TLR4 expression and downstream signals and to explore the molecular mechanisms of anti-inflammation by statins.Methods Human umbilical vein endothelial cells(HUVEC)were pretreated with atorvastatin(1 or 10 ?mol/L)or NF-?B inhibitor CAPE for 30 min,then incubated by purified LPS for 24 hours.TLR4,ICAM-1 and E-selectin mRNA were measured by RT-PCR;the percentage of TLR4 positive cells were detected by flow cytometry.The activation of NF-?B(p65)were detected by Western blot.Results Atorvastatin(1-10 ?mol/L)prevented LPS-induced increases in TLR4,ICAM-1 and E-selectin expression [TLR4 mRNA(1.24?0.21)vs LPS(1.82?0.27),P

The ethical education of laboratory medicine,as the indispensable part of modern medicine practice,hasn't been adequately emphasized,so that non-ethical things often ouur.This artide discusses the importance of ethical education on the field of laboratry medicine.