Objective: To observe the effect of verapamil and 6 chemotherapeutic agents vincristine,adriamycin,bleomycin,cyclophosphamide,5-Fu,cisplatin on the P-glucoprotein(P-gp) of the KBV200 cell line.Methods: Flow cytometer(FCM) assay was used to investigate the expression of P-gp on the KBV200 cell line in the different conditions.Results: Verapamil act with vincristine,adriamycin,pingyangmycin and cyclophosphamide separately decreased expression of P-gp on KBV200 cell line by 80.17%,89.69%,19.19% and 53.48% Among all the combinations,the synergistic effects of varapamil act with vincristine and adriamycine show a better effect.Conclusion: Verapamil act with vincristine,adriamycin,pingyangmycin and cyclophosphamide separately can decrease expression of P-gp on KBV200 cell line significantly,and can reverse the drug resistance of KBV200 cell line.

Objective: To make a study of KBV200 cell line on its multidrug resistance and provide experimental data for clinical individual chemotherapy.Methods:MTT reduction assay was used to investgate the inhibition of 8 common chemotherapetic agents on KBV200 cell line.Result:Vincristine、Adriamycin、Bleomycin、Cyclophosphamide and Arabinosyl Cytosine have poorer inhibition than Harrihgtonine、Cicplatin and 5-Fu on KBV200 cell line.Conclusion:KBV200 cell line is multidrug resistance to Vincristine、Adriamycin、Bleomycin、Cyclophosphamide and Arabinosyl Cytosine.

Objective To observe the inhibitory effect of 5 chemotherapeutic agents including vincristine,adriamycin,pingyangmycin,cisplatin,cyclophosphamide on the KBV200 cell line and to study the reverse effect on the multidrug resistance of Matrine.Methods MTT assay was used to investigate the inhibition ability of 5 drugs on the cell line and the reverse effect on the multidrug resistance of Matrine.Results Vincristine,pingyangmycin,cyclophosphamide had less inhibitory effects than adriamycin,cisplatin on the KBV200 cell line.Matrine had the ability to reverse the multidrug resistance of the cell line to vincristine,adriamycin.Conclusion KBV200 cell line is multidrug-resistant to vincristine,pingyangmycin,cyclophosphamide.Matrine can enhance the sensitivity of KBV200 to the vincristine,adriamycin.

OBJECTIVE:To study the pharmacokinetic profiles and bioequivalence of test ambroxol hydrochloride vs.refe-rence preparation.METHODS:18 healthy volunteers were administered with a single oral dose of test(ambroxol hydrochloride orally disintegrating tablets)and reference ambroxol hydrochloride(60 mg)by randomized crossover way.The plasma concentrations of ambroxol hydrochloride were determined by LC-MS.The pharmacokinetic parameters and the bioavailability were computed using DAS 1.0 program.RESULTS:The main pharmacokinetic parameters of test and reference ambroxol hydrochloride were as follows:Cmax:(84.7?29.3)and(81.2?28.2)ng?mL-1;tmax:(1.7?0.5)and(1.8?0.4)h;t1/2:(7.9?0.9)and(8.3?1.0)h;AUC0～24:(638.7?180.8)and(591.4?148.3)ng?h?mL-1;AUC0～∞:(717.5?211.1)and(671.1?173.2)ng?h?mL-1,respectively.The relative bioavailability of test ambroxol hydrochloride orally disintegratiog tablets was(108.2?16.5)%.CONCLUSION:The test and reference preparations of ambroxol hydrochloride were bioequivalent.

WIF-1 is an important antagonist in the Wnt/?-catenin pathway.As a tumor suppressor, WIF-1 is down-regulated in majority cancers but when it is correlated with WIF-1 promoter hypermethylation and treated with demethylation drug, its expression can be restored.Functional loss of WIF-1 due to promoter hypermethylation plays an important role in a tumor's pathogenesis, possibly mainly through aberrant canonical Wnt/?-catenin signal activation.The correlation of the expression of WIF-1 with clinicopathological features in different cancers is not identical.Detecting WIF-1 and promoter hypermethylation may offer help for early diagnosis, therapy and predicting prognoses.

Objective:To explore the efficacy of nimodipine combined with ganglioside in the treatment of cerebral hemorrhage and its effect on the expression of serum resistin and oxidized low-density lipoprotein (ox-LDL).Methods:According to the random number table method, 100 patients with cerebral hemorrhage admitted in Central Hospital of Zibo from February 2018 to February 2020 were divided into the single treatment group and the combined treatment group, with 50 cases in each group. In addition to conventional treatment, the single treatment group was additionally treated with nimodipine, and the combined group was additionally treated with nimodipine combined with ganglioside. After 2 weeks of intervention, the efficacy of the two groups was evaluated, and the National Institutes of Health Stroke Scale (NIHSS) scores, Ability of Daily Living (ADL) scores, serum inflammatory factors, resistin and ox-LDL levels were evaluated and recorded between the two groups before and after treatment.Results:The total effective rate of treatment in the combined treatment group was higher than that in the single treatment group: 90.0%(45/50) vs. 74.0%(37/50), and the difference was statistically difference ( P<0.05). After 2 weeks′ treatment, the scores of NIHSS was decreased and the scores of ADL was increased in two groups, and the scores of NIHSS in combined treatment group was lower than that in the single treatment group: (9.41 ± 1.27) scores vs. (12.10 ± 1.65) scores; the scores of ADL in the combined treatment group was higher than that in the single treatment group: (67.82 ± 9.04) scores vs. (59.10 ± 8.75) scores, and the differences were statistically differences ( P<0.05). After 2 weeks′ treatment, the levels of serum high-sensitivity C-reactive protein (hs-CRP), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), resistin and ox-LDL in the two groups were decreased, and the levels of above index in the combined treatment group were lower than those in the single treatment group: (4.81 ± 1.03) mg/L vs. (6.10 ± 1.73) mg/L, (40.96 ± 9.21) μg/L vs. (56.74 ± 8.93) μg/L, (33.20 ± 7.96) μg/L vs. (44.76 ± 8.43) μg/L, (0.29 ± 0.09) μg/L vs. (0.45 ± 0.13) μg/L, (336.25 ± 69.74) mg/L vs. (372.18 ± 68.52) mg/L, and the differences were statistically differences ( P<0.05). There was no significant difference in adverse reactions between the two groups ( P<0.05). Conclusions:Nimodipine combined with ganglioside has a clear efficacy in the treatment of cerebral hemorrhage. It can effectively inhibit inflammation indicators, reduce serum resistin and ox-LDL levels, promote the recovery of nerve defects, and improve their living ability.

Objective To investigate the effect of electroacupuncture plus early rehabilitation training on the function recovery after surgery for cervical spinal cord injury. Methods Sixty patients with cervical spinal cord injury undergone anterior cervical decompression and internal fixation were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by electroacupuncture plus rehabilitation training, while the control group was by rehabilitation training alone. The Frankel’s grading (FG), Barthel Index (BI), and Functional Independence Measure (FIM) were adopted for evaluation before and after intervention. Results The FG was not significantly changed after intervention in both groups (P>0.05). After intervention, there was no significant difference in comparing FG between the two groups (P>0.05). The BI and FIM scores were significantly changed after intervention in both groups (P＜0.01). There were significant differences in comparing BI and FIM scores between the two groups (P＜0.05). Conclusion Electroacupuncture plus early rehabilitation training can promote the function recovery after surgery for cervical spinal cord injury, and improve the quality of life.

AIM:To understand the differences between the extraction parameters of different parts from Acanthopanax. METHODS: The orthogonal design method was used in experiment arrangement; the influence caused by the different factors on the extraction of total flavone from the root and the stem of Acanthopanax senticosus Harms was obtained. RESULTS: The optimum extracting technical parameters for the root were as follows: 20 times of 50% ethanol to Acanthopanax senticosus Harms, refluxing for 3 h at 60 ?C . The optimum extracting technical parameters for the stem were as follows: 20 times of 50% ethanol to Acanthopanax senticosus Harms, refluxing for 4 h at 70 ?C. CONCLUSION: The optimum extracting technical parameters for the root were different from the stem, which is related to difference in the biological structures between the root and the stem of Acanthopanax senticosus Harms.

Objective To explore the effect of follow-up by telephone calls on the medication compliance of patients with cancer pain.Methods Two hundred and forty hospitalized cancer patients suffering from moderate to severe cancer pain were included in the study. All the patients were provided with education on pain treatment before discharge.Then they were randomly assigned to receive telephone follow-up or routine nursing after discharge.The medication compliance were compared 2 months after discharge.Result The medication compliance in the observation group was significantly better than that in the control group.Conclusion Telephone follow-ups can improve medication compliance in patients with cancer pain.

Resveratrol has several neuroprotective effects in cerebral ischemia, including reductions of oxidative stress, inflammatory responses and apoptosis. Recent studies have shown that these neuroprotective effects of resveratrol may be involved in activation of silent information regulator 1.