Objective To evaluate the safety of cardiac rehabilitation(CR)for the elderly patients with chronic heart failure(CHF). Methods All 72 patients with CHF over the age of 60 were enrolled and randomly divided into two groups: the experimental groups(n=35)and control groups(n=37). Patients in both groups were treated strictly according to the treatment guideline. On the basis of drug treatment,patients in experimental group were given a comprehensive CR program. The occurrence of all-cause death,due to deterioration of heart failure readmission and serious adverse events were compared after 12 months. ResultsCompared with the control group,the incidence of all-cause and the cases for the deterioration of heart failure readmission decreased in the experimental group after 12 months(P < 0.05). There was no statistically significant difference in the occurrence of serious adverse events between the two groups(P > 0.05). Conclusion For the elderly CHF patients,cardiac rehabilitation can effectively reduce all-cause death and deterioration of heart failure readmission in patients,but the serious adverse events had no obvious change. It is safe and effective for the elderly patients with chronic heart failure.

Objective In order to discuss the contribution and significance of lowering increased serum uric acid levels in cardio-vascular disease prevention and control .Methods All 100 hyperuricemia in senile patients with hypertension and diabetes mellitus were received the standard drug treatments for bloodpressure ,blood sugar management .According to the patients′will divided them into uric acid intervention group(study group) and control group .The study group were given low purine diet and benzbromarone tablet (50 mg/day ,course of 1 year) ,then compare the changes between the 2 group and within each group after 3months ,6months and 1 years in the metabolism indexes before and after intervention (serum uric acid ,glycosylated hemoglobin ,fasting blood glu-cose ,2-hours postprandial glucose ,and the changes of dynamic blood pressure ) ,and follow-up the occurrence of cases in primary end point events (all-cause death ,total cardiovascular death) .Results (1)In study group ,3months ,6months and 1 years after in-tervention ,the serum uric acid lever was significantly lower than that before intervention and the control group ,P<0 .01 .(2)The study group patients′dynamic blood pressure was significantly lower than that before intervention and the control group after 6 ,12 months ,meanwhile the study group patients′success rate of dynamic blood pressure level is higher than themselves before the in-tervention after 6 ,12 months ,P<0 .05 .(3)The 2 groups of patients′glycosylated hemoglobin ,fasting plasma glucose ,2-hours post-prandial glucose ,control rate of blood glucose at each testing point before and after the intervention had no difference .(4)The oc-currence of cases between the 2 groups for all-cause death and total cardiovascular death had no difference .Conclusion (1)Reduc-ing the mortality and disability rate of hyperuricemia in elderly patients with hypertension and diabetes mellitus ,the most critical measures is still a reasonable standard blood pressure and blood sugar management .Based on this therapy ,effectively reducing the increased serum level of uric acid can improve the control rate of blood pressure .

Objective:To analyze the changes of serum lipidomics in patients with sepsis and healthy controls, search for the differences of lipid metabolites, and reveal the changes of lipidomics in the process of sepsis.Methods:A prospective observational study was conducted. From September 2019 to April 2020, morning blood samples of upper extremity superficial veins were collected from 30 patients with definite sepsis diagnosed in intensive care unit (ICU) of Shanxi Bethune Hospital and 30 age-matched healthy subjects during the same period. Serum lipid metabolites were analyzed by ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS), and the quality control samples were analyzed by base peak spectroscopy (BPC) and verified experimental repetition. Student t-test and fold change (FC) were used for screening significant differences in lipid metabolites and determining their expression changes. Principal component analysis (PCA) and orthogonal projectionto latent structure discriminant analysis (OPLS-DA) were used to determine the entire allocation of experimental groups apiece, access the quality of being near to the true value of model, and screen the differential lipid metabolites with variable importance of projection (VIP). Finally, Metabo Analyst platform database was used to analyze lipid molecular metabolic pathways. Results:BPC results showed that the experimental repeatability was good and the experimental data was reliable. The main parameter model interpretation rate of PCA model R 2X = 0.511, indicating that the model was reliable. The main parameter model interpretation rate of OPLS-DA model R 2Y = 0.954, Q 2 = 0.913, indicating that the model was stable and reliable. With FC > 2.0 or FC < 0.5, P < 0.05, a total of 72 differential lipid metabolites were obtained based on VIP > 1. Based on Metabo Analyst 5.0, 24 distinguishable lipid metabolites were identified including 8 phosphatidylethanolamine (PE), 7 lysophosphatidylcholine (LPC), 6 phosphatidylcholine (PC), 2 lysophosphatidylethanolamine (LPE) and 1 phosphatidylserine (PS). Compared with healthy volunteers, the lipid molecules expression proved down-regulated in most sepsis patients, including PC, LPC, LPE, and some PE, while some PE and PS were up-regulated, which was mainly related to the PE (18∶0p/20∶4), PC (16∶0/16∶0) and LPC (18∶1) metabolic pathways in glycerophospholipids. Conclusions:There are significant differences in lipid metabolites between the sera of sepsis patients and healthy volunteers. PE (18∶0p/20∶4), PC (16∶0/16∶0) and LPC (18∶1) may be new targets for sepsis prediction and intervention.

Objective To investigate the intervention effect and mechanism of Suanzaoren decoction(SZRD)on insomnia and depression model mice based on pharmacodynamics and network pharmacology.Methods According to the insomnia disease model,the mice were randomly divided into control,insomnia model,SZRD low-dose,high-dose and diazepam groups;according to the depression disease model,the mice were randomly divided into control,depression model,SZRD low-dose,high-dose and Xiaoyao pill groups.The contents of 5-hydroxytryptamine(5-HT),norepinephrine(NE),and dopamine(DA)in the brains of mice were measured on days 7 and 28,and behavioral evaluations were performed in each group of mice.With the help of network pharmacology method to predict the key targets and related pathways of Suanzaoren decoction in the treatment of insomnia and depression,and to explore its mechanism.Results Behavioral tests and neurotransmitter content determination showed that SZRD could prolong sleep time(P<0.05),improve insomnia behavioral performance in model mice(P<0.05),reduce NE and DA contents in brain tissue of insomnia mice,increase 5-HT content(P<0.05),and was more significant in the low-dose group(P<0.05);SZRD could improve depression behavioral performance,increase sugar water preference,and reduce immobility time in model mice(P<0.05,P<0.01),and increase NE,DA,and 5-HT contents in brain tissue of depression mice(P<0.05),and was more significant in the high-dose group(P<0.05).18 core targets of SZRD in the treatment of insomnia and 34 core targets in the treatment of depression were predicted with the help of network pharmacology methods,and gene ontology(GO)functional analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that SZRD mainly involved biological processes such as dopamine neurotransmitter receptor activity,synaptic transmission and cellular response to reactive oxygen species,and acted on pathways such as the cGMP-PKG signaling pathway and IL-17 signaling pathway to exert therapeutic effects.Conclusion The changes of NE,DA and 5-HT contents in insomnia and depression model mice are not consistent,and the intervention effect of Suanzaoren decoction is also different.Low and high doses of Suanzaoren decoction can exert the best therapeutic effect on insomnia and depression model mice,respectively,and its promoting sleep and antidepressant effects may be related to the cGMP-PKG signaling pathway and IL-17 signaling pathway.

Objective: To investigate the immunity to mumps after administrating measles-mumps-rubella vaccine (MMR) among children aged 2-7 years old in Jiangsu province in 2015. Methods: A total of 4 190 healthy children aged 2-7 years old, living in local places for at least 3 months, and having been vaccinated at least 1 dose MMR were recruited to the study from Wujin district of Changzhou city, Gaogang district of Taizhou city and Ganyu district of Lianyungang city by using stratified cluster random sampling method between September and November, 2015. Those who did not accept MMR vaccination, who refused venous blood collection, who had affected mumps according to the memory of parents or teachers and who were diagnosed serious disease by clinical doctors were excluded from study. The self-designed questionnaire was used to collect the general information of the subjects and their MMR immunization history; and 0.5-2.0 ml of venous blood was collected from each subject. ELISA was used to detect the mumps antibody level in the serum of patients. Positive was defined as the antibody level ≥108 mU/ml, and negative as <108 mU/ml. χ² test was used to compare the difference in positive rates among subjects; and analysis of variance was used to compare the GMC changes in different time points after MMR vaccination. Results: Among 4 190 children, 2 280 were males (54.42%) and 1 910 were females(45.58%), and the positive rate of IgG antibody was 81.38% (3 344). There were 3 156 (95.18%) children vaccinated with one dose MMR, 187 (4.80%) children with two dose MMR, and 1 (0.02%) child with three dose MMR. The difference in positive rate of IgG antibody among different aged subjects showed statistical significance (χ²=58.61, P<0.001), the highest positive rate was in group of subjects aged 4-5 years old, at 89.43% (406/454), while the lowest positive rate was found among subjects aged 6-7 years old, at 75.63% (1 648/2 179). The positive rate after one dose of MMR vaccination was 79.14% (3 156/3 988), significantly less than it after two doses (93.03%, 187/201) (χ²=22.93, P<0.001). The GMC level at years<1, 1-<2, 2-<3, 3-<4, ≥4 following one dose MMR in the 3 988 children was 152.47, 227.78, 167.08, 126.91, 79.43 mU/ml, whose difference was statistically significant (F=51.29, P<0.001). Conclusion The sero-prevalence of IgG antibody in the children aged 2-7 years old in Jiangsu province was high. The positive rate among who received two doses MMR was significantly higher than it among who received just one dose, and the GMC level waned with times.