Through collecting and sorting of works, literature and medical cases regarding professor ZHANG Tangfa, it is found that his acupuncture academic ideology and clinical treatment of syndrome differentiation can be summarized as: tracing the source and paying attention to basic theory, especially the meridian theory and conception vessel and governor vessel; focusing on acupuncture manipulation and emphasizing acupuncture basic skills; highly valuing treating spirit, acquiring and maintaining needling sensation; underlining "three differentiations" that is consisted of syndrome differentiation, disease differentiation and meridian differentiation to guide the clinical prescriptions of acupoints; exploring and ingenious use of scalp acupuncture; being concerned on research of difficult and complicated diseases; advocating comparative studies to optimize the clinical treatment plan; proposing the combination of Chinese and western medicine, including diagnosis, treatment and basic theory, to improve the clinical therapeutic effects of acupuncture.
Acupuncture ; education ; history ; Acupuncture Points ; Acupuncture Therapy ; history ; methods ; History, 20th Century ; History, 21st Century ; Humans ; Meridians

Objective To observe the clinical efficacy of electroacupuncture in treating functional constipation. Method Sixty patients with functional constipation were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by electroacupuncture, while the control group was by sham-electroacupuncture. The Clinic Constipation Score (CCS), therapeutic efficacy index, plasma NOS, and plasma 5-HT were observed before and after intervention.Result The CCS was changed significantly after intervention in both groups (P<0.05). After intervention, there was a significant difference in comparing the CCS between the two groups (P<0.05). The total effective rate was 93.3% in the treatment group versus 66.7% in the control group, and the difference was statistically significant (P<0.05). The NOS and 5-HT contents were changed significantly after intervention in both groups (P<0.05). After intervention, there were significant differences in comparing the NOS and 5-HT contents between the two groups (P<0.05).Conclusion Electroacupuncture is an effective approach in treating functional constipation, and it can improve NOS and 5-HT contents in gastrointestinal tract.

The event free survival and overall survival rate of acute lymphoblastic leukemia (ALL)have been improved significantly based on the risk stratify diagnosis and treatment.But there are still some patients suffering therapeutic failure and relapse.With the great development of genome sequencing technology,more and more genetic aberrations behind the refractory and relapse ALL have been identified.Ph-like ALL is charactered with gene expression profile similar to that of BCR-ABL1 positive ALL,involving abnormal activation of cytokine receptor and tyrosine kinase.It has been proved that treatment combined with Tyrosine kinase inhibitors (TKIs) can significantly improve the poor prognosis.Ph-like ALL is one of hot topics during the 56th American Society of Hematology (ASH) annual meeting in 2014.Progression in molecular genetics for Ph-like ALL will be introduced together with the author' s research experience.

ObjectiveTo observe the clinical efficacies of electroacupuncture and Prucalopride succinate tablets in treating functional constipation.MethodForty patients were randomized into two groups, 20 cases in each group. The treatment group was intervened by electroacupuncture, and the control group was byoral administration of Prucalopride succinate tablets. The major symptoms scores, stool shape score, serum substance P (SP), and somotostatin (SS) were observed.ResultAfter treatment, there were no significant inter-group differences in comparing the changes of defecation frequency and difficulty scores (P>0.05), while there were significant inter-group differences in comparing the changes of incomplete bowel evacuation sensation, abdominal pain, and stool shape scores (P<0.05); the serum SP and SS levels were significantly changed after treatment in both groups (P<0.05); the total effective rate was 90.0% in the treatment group versus 85.0% in the control group, and the difference was statistically insignificant (P>0.05).ConclusionElectroacupuncture and Prucalopride succinate tablets can produce equivalent efficacies in treating functional constipation, and have their own advantages in improving the relevant symptoms. Therefore, treatment should be chosen according to the disease condition.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Constipation ; physiopathology ; therapy ; Defecation ; Humans ; Male

Objective To evaluate the sickness impact and the quality of life in patients who received 131 I treatment for Grave's disease with one year of follow-up.Methods 376 patients with Grave's disease(GD) who voluntarily received 131I treatment were recruited.The follow-up archives were established.The Sickness Impact and the Quality of life in patient' s with GD were measured using the Sickness Impact Profile (SIP),Self-Rating Anxiety Scale (SAS),Self-Rating Depression Scale(SAS) and Social Disability Screening Schedule (SDSS) and Quality of life scale(QLS,SF-36) before and after treatment with 131I for 6 months and 12 months.Results 57 out of 376 cases were lost.319 cases finished follow-up studies.There was significant difference of SAS,SDS,SDSS,SIP and SF-36 and their agent score among three groups:before and 6months and 12months after 131I treatment in the 319 patients(F=8.561-1080.317,P＜0.001).After treatment with 131I,SAS,SDS,SDSS and SIP score were lower(P＜0.05),SF-36 total and agent score were higher(P＜0.05).There was no significant difference between the score of SAS,SDS,SDSS,SIP total score and it' s agent score of SD-Ⅱ,SR,W,SF-36 agent score of RP,BP,VT,SF at the end of 12 months compared to the score at the end of 6 months(P＞0.05).But there was significant difference between the score of SIP agent score of SD-Ⅰ,HM,RP,SF-36 total score and it' s agent score of PF,GH,RE,M H at the end of 12 months compared to the score at the end of 6 months (P＜0.05).At the end of 6 months and 12 months after treatment the subjects were divided five groups according to different clinical outcome.Not only at the end of 6 months,but also at the end of 12 months,there was significant difference of SAS,SDS,SDSS,SIP total score,and it' s agent score of SD-Ⅰ,SD-Ⅱ,SR,W,RP,SF-36 total score,and it's agent score PF,RP,BP,GH,VT,SF,RE,MH among the five groups(F6 =6.870-143.790,F12 =13.956-837.184,P＜0.001).There was no significant difference of HM among five groups (F6 =1.733,P6 =0.142; F12 =2.015,P12 =0.092).The score of SF-36 and its agent score PF,RP,VT,SF,RE,MH in three subgroup (healthy control,the patient group at end of 6 months and 12 months with normal thyroid function) was significant different,respectively(F=8.320-82.791,P＜0.001).There was no significant different for agent score of BP and GH(F=2.990,2.652,P=0.051,0.072).Conclusion Quality of life of patients with GD is decrease.131I treatment can improve it,but socialpsycho function can not be improved satisfactorily.It is necessary for GD patients to pay attention to the quality of life and provide effective mental intervention to improve the recovery completely.

The integrator complex is multifunctional and contains at least 12 evolutionarily conserved subunits in hu-mans.It interacts with the C-terminal tail of the largest subunit of RNA-polymeraseⅡ ( RNAPⅡ) to promote 3′-end pro-cessing of small nuclear RNA (snRNA) U1/U2.It also interacts with RNAPⅡ, NELF and Spt5 to regulate NELF-mediated RNAPⅡpause/release and processivity at coding genes .Recently, the integrator complex is also reported to be involved in DNA damage response , dynein recruitment to the nuclear envelope , integrity of Cajal bodies , adipose differentiation , hem-atopoiesis , ciliogenesis , tumorigenesis and generation of viral microRNAs .This review discusses related research progress in the integrator complex .

The efficacy of hematological tumor drugs has shown inter-patient variability. Pharmacogenomics focuses on gene polymorphisms of drug metabolizing enzymes, drug transporters and therapeutic targets and its impact on pharmacokinetics (PK)/pharmacodynamics (PD). Initial dose and adverse reaction can be predicted based on inherited gene polymorphisms, and therapeutic drug monitoring (TDM) also helps to adjust drug dosage in the course of treatment. Thus can achieve rational drug use and personalized medicine, and improve the efficacy and reduce the incidence of adverse drug reactions. This article will introduce the relevant research progress in recent years, and the concept of personalized medication fingerprints is proposed.

The detection of JAK2 V617F and CALR mutations,which was first reported in 2005 and 2013,respectively,had greatly improved the diagnosis and treatment of myeloproliferative neoplasms (MPN).Most MPN patients are inclined to thrombophilia,the carrier frequency of JAK2 V617F mutation was also high in patients of various thrombotic events,and the relationship between the two conditions needs further study.The observation of JAK2 V617F mutation in control group and general population also enlightened and changed the understanding about the development of chronic cancer.Research progress in this area will be introduced together with the related reports in the 56th American Society of Hematology annual meeting in 2014.

Objectives To detemline whether apoptotic cell death is involved in rat cardiac allograft rejection and investigate the relevance of apoptosis with acute rejection and its implication.Methods Groups of Wistar rats underwent heterotopic heart transplantation from allogeneic SD or syngeneic Wistar rats.The cardiac grafts were harvested at 1,3,5,or 7 days after transplantation and underwent the detection of apoptotic cell death using in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling(TUNEL).Histopathological rejeclion grade and apoptotic index(AI)were analyzed.Results The incidence of apoptotic cells was increased steadily over time in allografts,in contrast to syngeneic grafts.The apoptotic cells in allografts were mainly cardiac myocytes and few infiltrating lymphocytes.The AI of rejection grade 1,2,3 and 4 was significantly higher than that of rejection grade 0(P<0.01).Conclusions TUNEL can display apoptosis of single cell in situ.Apoptosis is an important mechanism of tissue injury in acute cardiac allograft rejection in rats.Myocyte apoptosis can be used as a valuable index to estimate the injury of grafts and monitor acute rejection.