1.Lib2.0-based subject service platform construction in Library of Second Military Medical University
Chinese Journal of Medical Library and Information Science 2014;(5):12-15
After the connotation and application of Lib2.0 technology were described, how to construct the subject service platform in Library of Second Military Medical University using Lib2.0 technology was elaborated and the service-oriented subject service platform construction plan was proposed .
2.Effects of isoproterenol on myocardial fibrosis and related cytokines in rats
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(10):-
AIM:To study the effects of isoproterenol (Iso) on cardiac fibrosis and related cytokines in rats. METHODS:20 SD rats were divided into control and Iso group in random, with 10 rats each group. After injected Iso, cardiac histology was observed and cardiac function, collagen volume fraction (CVF), content of collagen protein and the concentrations of Angiotensin Ⅱ (Angll) and nitrogen monoxide (NO) were measured in the two groups. RESULTS:Compared with control group, microscopic examination showed that fibrosis increased in Iso group. Iso did decrease cardiac function and NO levels and increase CVF, content of collagen protein and AngII levels. Left ventricular pressure maximal rate of rise (+ dp/dt_(max)) and left ventricular systolic pressure (LVSP) were not significantly different from that of control group. CONCLUSIONS:Iso can induce myocardial fibrosis and change levels of related cytokines in rats.
3.Erythrocyte membrane Na-pumping K~+-pNPPase activity and red cell deformability in diabetes mellitus
Chinese Journal of Pathophysiology 1989;0(06):-
The activity of erythrocyte membrane Na-pumping K~+-activated pera-nitro-phenylphosphatase (K~+-pNPPase) and red cell deformability (RCD) were stndied in 50adult patients with non-insulin-dependent diabetes mellitus (NIDDM). RCD was expressedas red blood cell filtration index (RBC-IF). In comparison with the healthy subject, theactivity of erythrocyte membrane Na-pumping K~+-pNPPase was significantly decreased,and RBC-IF was significantly increased. The above-mentioned changes were more severein NIDDM with retinopathy than those without. There was a very close negative corre-lation between Na-pumping K~+-pNPPase and RBC-IF. These results indicated that thedecrease of erythrocyte membrane Na-pumping K~+-pNPPase activity might be an importantfactor fot the decrease of RCD.
4.Determination of Estrogens in Water by Solid Phase Extraction-High Performance Liquid Chromatography
Qian YU ; Hongxin WANG ; Ke AN
Journal of Environment and Health 1992;0(05):-
0.998. The detection limits were in the range of 16-40 ng/L(S/N=3). The quantitative detection limits were in the range of 45-105 ng/L(S/N=10) with recovery percentages ranging from 87.6% to 106% and the relative standard deviations was lower than 3%. Conclusion The method was proved to be satisfactory in precision,accuracy and sensitivity and applicable to the daily test of estrogens in water.
5.Ca~(2+)/calmodulin-dependent kinaseⅡare involved in tumor necrosis factor α-induced cardiomyocyte hypertrophy in rats
Guijun WANG ; Yusheng YAO ; Hongxin WANG
Chinese Pharmacological Bulletin 2010;26(3):387-391
Aim To investigate whether Ca~(2+)/calmodulin-dependent kinase Ⅱ(CaMKⅡ)contribute to tumor necrosis factor α(TNF-α)-induced cardiomyocyte hypertrophy.Methods The protein content was assayed with Lowry's method.The cardiomyocytes volumes were measured by computer photograph analysis system.The protein synthesis was assayed with[~3H]-lencine incorporation method.[Ca~(2+)]_i transient was measured by Till image system by cell-loading Fura-2/AM.The expression of CaMKⅡδ_B was determined by Western blot.Results ① TNF-α significantly induced the increase of protein content, [~3H]-leucine incorporation and cell size;These responses were significantly suppressed by KN93, a selective CaMKⅡ inhibitor.② TNF-α increased the amplitude of the spontaneous Ca~(2+) transients in cultured ventricular myocytes from the neonatal rat;CaMKⅡ inhibitor KN93 can suppress the elevation induced by TNF-α.③ TNF-α significantly increased the expression of CaMKⅡδ_B.Concluslon CaMKⅡ signal pathway are involved in TNF-α-induced cardiomyocyte hypertrophy in rats.
6.Ca~(2+)/calmodulin-dependent kinaseⅡare involved in tumor necrosis factor ?-induced cardiomyocyte hypertrophy in rats
Guijun WANG ; Yusheng YAO ; Hongxin WANG
Chinese Pharmacological Bulletin 1987;0(03):-
Aim To investigate whether Ca2+/calmodulin-dependent kinase Ⅱ(CaMKⅡ)contribute to tumor necrosis factor ?(TNF-?)-induced cardiomyocyte hypertrophy.Methods The protein content was assayed with Lowry's method.The cardiomyocytes volumes were measured by computer photograph analysis system.The protein synthesis was assayed with[3H]-lencine incorporation method.[Ca2+]i transient was measured by Till image system by cell-loading Fura-2/AM.The expression of CaMKⅡ?B was determined by Western blot.Results ① TNF-? significantly induced the increase of protein content,[3H]-leucine incorporation and cell size;These responses were significantly suppressed by KN93,a selective CaMKⅡ inhibitor.② TNF-? increased the amplitude of the spontaneous Ca2+ transients in cultured ventricular myocytes from the neonatal rat;CaMKⅡ inhibitor KN93 can suppress the elevation induced by TNF-?.③ TNF-? significantly increased the expression of CaMKⅡ?B.Concluslon CaMKⅡ signal pathway are involved in TNF-?-induced cardiomyocyte hypertrophy in rats.
7.Analysis of clinical research literature about treated plague by using traditional Chinese medicine
Le WANG ; Huamin ZHANG ; Hongxin CAO
International Journal of Traditional Chinese Medicine 2011;33(6):521-523
Objective To analyze the problems of treating plague by TCM and find out the characteristics of clinical research of contemporary plague by the analysis of clinical research literature on the treatment of plague by TCM. Methods Differential treatment methods of one kind of plague in the literature as the main source of the data and the information that the pathogenesis-symptom-location of disease-nature of disease-prescriptions-drugs were taken to build the data base of the syndrome differentiation and treatment of certain kind of plague. Results Analyzed with literatures,the characteristics of plague clinical research showed: Different pathogenesis characteristics and evolution of plague were caused by diff① erent evils. ②Contemporary plague was named by western disease name. A new model which disease differentiation combined with syndrome differentiation has been formed. ③The diversification of administration promoted the treatment of emergent communicable disease with Chinese medicine. Conclusion The plague research lacked such research literature which could both reflect individualized diagnosis and embody the clinical rules. Traditional Chinese medicine theory could be researched and enriched by finding out the pathogenesis characteristics and evolution rule of the new plague and thus guide the clinical practice. It was effective to control and treat mergent communicable disease with TCM.
8.The analysis of the telephone counseling contents of 5202 person-times in psychological hot line
Dengqin WANG ; Jingxuan ZHANG ; Hongxin WU
Chinese Journal of Behavioral Medicine and Brain Science 2001;10(1):58-60,62
Objective The purpose of the study is to analyse the counseling contents in psychological hot line during four years and the characteristics in different groups of the people. Methods Inputting all the completed recorders in hot line from January, 1995 to December, 1999, and using the Statistic Analysis System to analyse the data. Results The main age groups of the counseling people are 20~years and 30~years (77.39%), and 87.43 percent of the people has good education. The main counseling contents are related to mental disorders (23.61%), emotional difficulty (19.74%), love (17.07%), social intercourse (9.69%), marriage (9.15%), consulting for other persons (7.67%), sexual problems counseling (6.36%), and others. Conclusions The main groups of people in hot line are young and good educated people. The counseling contents have touched upon many areas, but mental disorder is the most numerous problems. The main problems in different groups of people are marked difference.
9.Effects of pioglitazone on cardiac fibroblast proliferation induced with high glucose and high insulin
Keyan LIANG ; Hongxin WANG ; Pin BAO
Chinese Pharmacological Bulletin 2003;0(10):-
Aim To study effects of pioglitazone on cardiac fibroblast proliferation induced with high glucose and high insulin.Methods The neonatal rat cardiac fibroblasts were cultured and treated with different factors.The cellular number was determined by crystal violet uptake;the DNA synthesis was assayed with thymidine intake method;the percentage of S+G_2+M in the cell cycle was determined by flow cytometry.Results Pioglitazone inhibited the cell number、DNA synthesis and the percentage of S+G_2+M in the cell cycle of cultured cardiac fibroblasts induced with high glucose and high insulin.Conclusion Pioglitazone inhibits cardiac fibroblast proliferation induced with high glucose and high insulin and the effective mechanism of pioglitazone on cardiac fibroblast proliferation needs further study.
10.Study on mechanisms of ?-opioid receptor agonist affects neonatal rat myocardial hypertrophy induced by isoproterenol
Lei CUI ; Hongxin WANG ; Min ZHAO
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(09):-
AIM:To study the mechanisms of effects of the ?-opioid receptor(?-OR) on neonatal rat hypertrophic myocardial cells induced by isoproterenol(ISO).METHODS:Myocardial cells of neonatal rats were cultured in vitro.The total protein content was detected by the Lowry's assay.The volume of myocardial cell was measured by computer photogragh analysis system and the protein synthesis was assayed with[3H] leucine intake method.The degree of extracellular signal-regulated kinase(ERK) phosphorylation was determined by western-blotting.RESULTS:U0126(an inhibitor of ERK) decreased the total protein content,the cell size,the[3H]leucine incorporation and the ERK phosphorylation degree in hypertrophic myocardial cells induced by ISO.1 ?mol/L U50,488H(a ?-opioid receptor antagonist) could decrease the total protein content,the cell size,the[3H]leucine incorporation and the degree of ERK phosphorylation.But these effects could be diminished when incubated with U0126.CONCLUSION:?-opioid can abolish the hypertrophic response induced by ISO of cardiac myocytes,which was associate with ERK pathway.The inhibitor of ERK significantly inhibits the ISO-induced hypertrophied effect.