Objective To evaluate the diagnostic value of enlargement of the anterior space of the right branch of portal vein in early cirrhosis.Methods 37 cases with early cirrhosis and 42 cases with type B hepatitis proved by pathology as well as 40 health cases underwent MDCT examinations.The width of the anterior space of right branch of portal vein(RBPV)and the diameters of RBPV in each group were measured on CT imaging,then the results were analysed comparatively.Results The width of anterior space of RBPV and the diameters of RBPV were(5.5±2.4)mm and(11.84±1.91)mm in health group,(13.6±5.8)mm and(12.36±61.97)mm in early cirrhosis group,(5.7±3.1)mm and(12.13±1.89)mm in type B hepatitis group,respectively.There were significant differences in the width of the anterior space of RBPV between three groups(P＜0.0001).However,there were not significant differences in the width of RBPV among these three groups(P＞0.05).While,there were significant differences of the width of the anterior space of RBPV and the diameters of the RBPV between normal control and type B hepatitis(P＞0.05).Conclusion CT sign of the anterior space of RBPV increased is of important value in diagnosis of early cirrhosis.

According to the recommendations of the International Medical lnformatics Association(IMIA)on education in biomedical and health informatics(first revision)published by IMIA in 2010 and combining the actuality of the basic clinical information skills and medical informatics education of Chinese medical students,the related contents of medical informatics have been integrated into the teaching course based on current medical information retrieval curriculum and medical informatics curriculum for non-medical informatics students of medical colleges and universities was constructed,so that they can meet the requirements of the basic information skills under information-based medical and healthy practice.

Objective To explore the mechanism of nanometer calcium combined with tonifying kidney herbs on osteoporosis by studying the expression of CaBP-D9K mRNA and protein in the intestinal mucosa of osteoporosis rats due to kidney deficiency. Method The rat model of osteoporosis due to kidney deficiency was established by ovariectomy. Applying the nanometer calcium and nanometer calcium combined with tonifying kidney herbs (big, middle and small dosage) to the rats for 12 weeks, choosing Gushukang, E2 and Gai’erqiD600 as the control drugs. The expression of the CaBP-D9K mRNA and protein in the intestinal mucosa of the osteoporosis rats due to kidney deficiency was determined by the method of RT-PCR and western blotting. Result There was CaBP-D9K and protein expression in the normal rats intestinal mucosa tissue, which plays an important role in the process of the intestinal calcium absorption. The episode of the rat osteoporosis due to kidney deficiency is related to the change of the CaBP-D9K and protein expression and has influence on the intestinal calcium absorption. Conclusion The nanometer calcium combined with tonifying kidney Chinese herbs can stimulate the calcium absorption of intestine, prevent and treat the osteoporosis due to kidney deficiency through regulating the expression of CaBP-D9K mRNA and protein in the intestinal mucosa.

Objective To study the reliability and validity of clinical experts questionnaire of syndrome factors, characteristics and evolution of coronary heart disease. Methods The clinical expert questionnaire was sent to 305 clinical doctors of cardiovascular disease. The questionnaire of coronary heart disease was described through four phases data, and reliability and validity of each module and the total scale were tested. Results Cronbach's ? coefficient of the modules varies between 0.959 3～0.972 5 and higher than 0.8, the value of the scale was 0.990 3. Split-half reliability of the modules varies between 0.891 8～0.922 5, Cronbach's ? of split-half reliability of the total scale was 0.920 7. Factor analysis showed that each module was abstracted four factors and the total contribution rate was more than 50%, the result after varimax rotation was consistent with the theory. Conclusion The questionnaire can fully reflect symptoms and signs of patients with coronary heart disease, and can be used for syndrome studies of coronary heart disease.

ObjectiveTo investigate the expression of stem cell marker adenosine triphosphate (ATP)-binding cassette transporter 2 (ABCG2) in the tissue and side population (SP) of a cell line A431 of human cutaneous squamous cell carcinoma(SCC).MethodsSP cells were separated by flow cytometry from cultured A431 cells.Methyl thiazolyl tetrazolium(MTT) assay was used to evaluate the proliferative ability of,reverse transcription-PCR to determine the expression of ABCG2 in,SP and non-SP cells.Immunohistochemistry (MaxVision method) was carried out to detect the expression of ABCG2 protein in tissue specimens from 10 patients with SCC.ResultsSP cells existed in cultured A431 cells,and accounted for about 1.1％ of A431 cells.The SP cells had a stronger growth and colony-forming ability than non-SP cells did.The number of cell clones formed by SP cells and non-SP cells was 114.8 ± 4.95 and 44.5 ± 3.67,respectively,per well in a 24-well plate( t =27.92,P ＜ 0.01 ).The expression level of ABCG2 mRNA was significantly higher in SP cells than in non-SP cells(t =5.22,P＜ 0.01).There existed a small number of ABCG2 positive cells in SCC tissue,and ABCG2 was mainly expressed in the cytoplasm and membrane of tumor cells.ConclusionsSP cells exist in A431 cells,which have characteristics of stem cells and highly express ABCG2.ABCG2 may be a potential stem cell surface marker of SCC.

Objective To investigate the effect of Aurora-A kinase inhibitors VX-680 on the proliferation of thyroid carcinoma cell and to study their molecular mechanism. Methods The undifferentiated thyroid carcinoma cells were divided into groups of VX-680 with different drug concentration. Drug effect was observed by MTT method to measure the changes of cell proliferation. The gene expression of Aurora-A , Bcl-2 and Bax were tested by immunofluorescence assay. Results MTT results showed cell proliferation had been suppressed obviously with the of concentration increase of drug and the duration of administration (P < 0.05). The immunofluorescence results of 48 h showed Aurora-A and Bcl-2 protein expression of 400 nmoL were lower than those of other groups (P < 0.05), and Bax protein expression was similar to that of other groups(P > 0.05). Conclusions Aurora-A and Bcl-2 protein expression of thyroid cancer are inhibited when VX-680 being used , at the same time , Bcl-2/Bax scale value loses balance to accelerate forming Bax/Bax homodimer. Thus cancer cell proliferation is blocked and cell apoptosis is induced. So VX-680 is a good target inhibitor for anaplasia thyroid cancer.

This study was aimed to observe four kinds of kidney-tonification medicine, which were Epimedium, pso-ralen, Ligustrum lucidum, Polygonum with the active ingredient of icariin, psoralen, oleanolic acid, stilbene glucoside and their orthogonal compatibility. There were two kinds of non-kidney tonification medicine, which were Chuanx-iong and astragalus with the active ingredient of TMP and astragaloside. The observation was made on the regulatory role of rat bone marrow stem cells (BMSCs). A total of 65 SD rats were randomly divided into the normal control group, positive transformed control group, kidney-tonification compatibility group (including Group 1, Group 2, Group 3, Group 4, Group 5, Group 6, Group 7, Group 8, and Group 9), non-kidney tonification medicine control group (in-cluding TMP group and astragaloside group). Intragastric administration of medication was given to the kidney-tonifi-cation compatibility group and the non-kidney tonification medicine control group, once a day for 3 consecutive days. Intragastric administration of equal amount of normal saline was given to the normal control group and the posi-tive transformed control group. On the third day of intragastric administration, rats in each group were sacrificed. Serum containing medication was used in the culture of BMSCs for 6, 12, or 18 days. ELISA method was used to quantitatively detect the expression activity and content of BMP2 on the 6th, 12th, or 18th day, in order to evaluate the degree of bone cell differentiation degree. Real-time quantitative PCR method was used for detection of expression of Bmp2, Smad1, Smad4 mRNA in serum containing medication in the culture of BMSCs on the 18th day. The results showed that the kidney-tonification compatibility can improve the expression activity and content of BMP2 culture in vitro, with the peak on the 12th day. The kidney-tonification compatibility groups can upregulate expressions of Bmp2, Smad1, Smad4 mRNA. It was concluded that the active ingredient compatibility of kidney-tonification medicine can promote BMSCs. Its mechanism may be related to the upregulation of expression of Bmp2, Smad1, Smad4 mRNA, and the activity and content of Bmp2.

Objective To investigate the expression of CD200 in peripheral lymphocytes and hair follicles from patients with alopecia areata (AA).Methods Flow cytometry was used to detect the expression of CD200 in peripheral lymphocytes from 43 patients with AA and 43 healthy controls.Immunohistochemistry was carried out to quantify the expression of CD200 and cytokeratin 15(CK15,a marker for basal cells of the outer root sheath) in resected scalp specimens from 8 patients with AA and 8 healthy controls.Differences in the expression of CD200 and CK15 between the patients and controls were assessed by independent-samples t test and rank sum test.Data were processed by the software SPSS17.0.Results The percentage of CD200-expressing cells in peripheral blood lymphocytes and T lymphocytes was significantly lower in the patients with AA than in the healthy controls (5.73％ ± 3.46％ vs.12.01％ ± 4.90％,8.85％ ± 4.80％ vs.12.31％ ± 3.12％,t =6.865,3.964,respectively,both P ＜ 0.05).However,no significant difference was observed in the percentage of CD200-expressing cells in peripheral blood B lymphocytes between the patients and controls (74.68％ ± 8.12％ vs.75.75％ ± 9.45％,t =0.570,P ＞ 0.05).Further more,the patients showed a lower expression of CD200 (P ＜ 0.05),but a similar expression of CK15 (P ＞ 0.05) in hair follicles compared with the controls.Conclusion The decrease in CD200 expression in peripheral lymphocytes and hair follicles may be involved in the pathogenesis of AA.

Objective To observe the effects of nourishing-kidney herbs on rat model with glucocorticoid-induced osteoporosis.Methods 60 Wistar rats male and female half and half were randomly divided into a normal group,a model group and a nourishing-kidney group.By intramuscular injection of dexamethasone (2.5 mg/kg) twice a week to replicate osteoporosis rat model.All groups were treated for 9 weeks.Detected the BMD of femur in vitro and determined the bone metabolism marker in serum by biochemical process.Results The BMD decreased obviously (0.109± 0.007)g/cm2 and the content of TRAP in serum increased evidently (9.96± 1.15) μg/ml in the model group.In the nourishing-kidney group,the BMD was up-regulated (0.116 ± 0.007)g/cm2,and TRAP down-regulated(5.76 ± 0.85)μg/ml.Conclusion Intramuscular injection of dexamethasone can induce GIO rat model,and nourishing-kidney herbs have the effect of anti-osteoporosis.

OBJECTIVETo set up a rapid and simple method for identificating bacteria by 16S-23SrDNA intergenic spacer regions (ISRs).

METHODSPolymorphic products of PCR from ISRs were selected on agarose gel and sequenced directly using purified fragments by excising the gel without cloning. Nucleotide sequences were compared with GenBank databases and analyzed by DNAMAN program.

RESULTSThere was only a single product in streptococcus genus after PCR amplification of 16S-23SrDNA ISRs. Five streptococcal species were obtained from 7 strains of streptococcus. Two major amplicons were consistently generated for 8 strains of Haemophilus influenzae (H. influenzae). The sequence data showed that they all belonged to H. influenzae type b on GenBank databases.

CONCLUSIONPCR and direct sequencing of 16S-23SrDNA ISRs were very successful methods for bacterial species identification.

Base Sequence ; DNA, Ribosomal Spacer ; chemistry ; Haemophilus influenzae ; genetics ; isolation & purification ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; methods ; RNA, Ribosomal, 16S ; genetics ; RNA, Ribosomal, 23S ; genetics ; Streptococcus ; genetics ; isolation & purification