Objective:To investigate the expression and significance of ABCG2(the G2 member of ATP-binding cassette family)in U251and its stem cell.Methods:The U251 were cultured in serum-free medium with EGF,bFGF,LIF and B27.The tumor spheres formed and cultured continuously in the serum-free medium through the monoclonal formation experiment.The immunofluorescence staining was employed to identify the U251 stem cell.The expression of ABCG2 was detected by confocal microscopy and Western blot.Results:The stem cells from malignant glioma cell line U251 have been successfully isolated.The cells expressed CD 133.The ABCG2 protein positive stain was located in the cell membrane in both U251 cells and its stem cell with confocal microscopy method.Western blotting showABCG2 was expressed in U251 cells and in its stem cells.The expression level in the stem cells was significantly higher than U251 cells.Conclusion:ABCG2 is expressed in U251 cells and in U251 stem cells.The expression level in the latter was higher.

In the past five thousand years,the advanced theory and effective practice of traditional Chinese medicine have played a key role in the prosperity and population growth of traditional Chinese people.This article analyzed the opportunities and challenges that traditional Chinese medicine faces in its current state of development and discussed the progressiveness of traditional Chinese medicine theory and practices.The author advocates that "the essential task of developing traditional Chinese medicine is to preserve and expand the progressiveness of traditional Chinese medicine theory and practices".In the 21 century,human health concept and medicine model have been changing.And,the fast pace development in modern technology and our country's growing emphasis in traditional Chinese medicine offer a great opportunity for traditional Chinese medicine to evolve.In such a juncture,the author believes that the key to the further development of traditional Chinese medicine rests in the correct understanding,preservation and expansion of the advanced traditional Chinese medicine theory and practices.To do so,we need to focus on "fundamental development" and "independence innovation" aiming at using traditional Chinese medicine to continuous improving our capability not only in disease prevention and treatment but also in science and technology innovation to allow traditional Chinese medicine to make more contribution in protecting human health.

To investigate the relationship between hyperglycemia and hyperlipidemia in diabetics, eighty-six patients with non-insulin dependent diabetes mellitus (NIDDM) were followed up for 6 to 12 months. The results showed that plasma TC, TG, LDL-C levels and LDL-C/HDL-C ratio were elevated in diabetic group as compared with normal control group. Plasma TC and LDL-C levels in the diabetics with mean fasting blood glucose (mFBG) level more than 150 mg/dl were significantly higher than those in diabetics with mFBG less than 150 mg/dl, and those in normal control group as well, but plasma TC, LDL-C and TG levels showed no significant difference between diabetics with mFBG less than 150 mg/ dl and normal controls.The disturbances of lipid metabolism were obviously alleviated following the improvement of diabetic control. These results suggest that better diabetic control can be beneficial to the improvement of lipid metabolic disorders and might be helpful to the prevention of angiopathy in NIDDM patients.

The relationship between plasma magnesium and plasma lipids was studied in 80 cases of Type II diabetes mellitus. It was found that plasma magnesium in diabetic patients was much lower than that in normal controls. Plasma magnesium was negatively correlated with plasma cholesterol, triglyceride, and LDL-C, and positively correlated with plasma HDL-C, HDL2, and HDL-C/LDL-C, suggesting that hypom-agnesemia was closely related with disturbance in lipid metabolism in diabetes mellitus. Hypomagnesemia seemed to worsen the disturbance of lipid metabolism, and might be one of the factors causing vascular complications in diabetes mellitus

OBJECTIVE:To compare the clinical efficacy and safety of Shexiang baoxin pills and Shensong yangsin capsules in the treatment of coronary heart disease complicated with chronic heart failure and premature ventricular contractions. METHODS:129 patients with chronic heart failure and premature ventricular contractions were randomly divided into blank group(n=43),ob-servation group(n=43)and control group(n=43). All groups received routine treatment as oxygen inhalation,diuretics. Control group additionally received Shensong yangsin capsules 4 pills for 3 times per day. Observation group additionally received Shexiang baoxin pills 2 pills for 3 times per day. Treatment course of 2 groups lasted for 3 months. Clinical efficacies of 2 groups were ob-served,and heart function,cardiovascular autonomic nervous function and vascular endothelial function were observed before and after treatment. RESULTS:Total effective rate of observation group was significantly higher than that of control group(P<0.05). SDNN,SDANN,RMSSD,QTd was no statistical significance between control group and observation group(P>0.05). LVEF, NO of observation group were significantly higher than that of control group(P<0.05),IVST,LVDd,LVPWT,ET of observation group were significantly lower than that of control group(P<0.05). But control group and observation group were better than the blank group,and no obvious adverse reaction. CONCLUSIONS:Shexiang baoxin pills is more effective than Shensong yangsin cap-sules in the treatment of coronary heart disease complicated with chronic heart failure and premature ventricular contractions.

OBJECTIVE:To observe the clinical efficacy and safety of Mailuoning injection in the treatment of acute bronchial asthma. METHODS:92 patients with acute bronchial asthma were randomly divided into control group and observation group. Pa-tients in control groups were received routine treatment,including oxygen inhalation,glucocorticoid,β2 agonists,aminophylline and anti-infection,etc. Patients in observation group were added Mailuoning injection 20 ml adding into 5% glucose injection or 0.9% sodium chloride injection 500 ml by intravenous infusion,once a day,based on the treatment of control group. The course was 14 d. The clinical data was observed,including clinical efficacy,hemorheology indicators,pulmonary function,6-keto-PGF1αlevel and incidence of adverse reactions before and after treatment. RESULTS:The total effective rate in observation group was sig-nificantly higher than control group,with significant difference(P<0.05). After treatment,the hemorheology indicators,lung func-tion indicators and 6-keto-PGF1α level in 2 groups were significantly better than before,and observation group was better than con-trol group,with significant difference(P<0.05). There were no obvious adverse reactions. CONCLUSIONS:Routine treatment combined with Mailuoning injection can effectively improve the efficacy of acute bronchial asthma with good safety.

Objective To study the regulating effect of complementing calcium and invigorating kidney method on the bone morphogenetic protein-4(BMP-4) and signal transduction mechanism.Methods Female Wistar rats were randomly divided into seven groups:normal group,model group,low-,middle-,high-dose yster calcium compound Chinese medicine for reinforcing kidney group,Gushukang granule group,oyster shell calcium tablets group.The model of postmenopausal osteoporosis was established through ovariectomy in rats.The osteoporosis rats were treated with nanometer calcium and invigorating kidney Chinese medicine Gushukang granule,and oyster shell carbonate chewable tablets were used as positive-control groups,the normal rats as standard control,and the model rats as model control.After 12 weeks of the treatment,the indexes were tested.The bone mineral density(BMD) of femoral bone in vitro was detected by dual-energy X-ray densitometer and the expression of BMP-4 and Smad 5,6 mRNA were detected by reverse transcription-polymerase chain reation(RT-PCR).Results Compared with the model rats,BMD of femoral bone in Chinese medicine groups(high dose,middle dose,low dose) increased obviously(P

By incorporation of tritiated thymidine or 5-bromodeoxyuridine and using them as the markers,we found that the basal cells,Clara cells and type II alveolar epithelial cells in the pulmonary tissues may in some way behave like stem cells and can eventually differentiate into respiratory tract epithelium.The slow progress of researches on pulmonary stem cells up to date has its root in the complex structure of pulmonary tissues and respiratory tracts,which is demonstrated by the facts that the respiratory system is composed of at least 40 types of cells,plus that the growth,renewal,and regeneration rate or capacity of respiratory epithelium are extremely limited.Mean while,there have been no specific stem cell markers for pulmonary and lung cancer,so researchers rely on stem cell markers borrowed from other systems to search for stem cells in pulmonary system.At present,the studies of lung stem cell markers were focused on Sca-1,ABCG2 /Bcrp1,cell retaining marker,surface marker,cell keratin and CCSP.Recently the lung stem cells have been shown to be the targets of transformation during lung carcinogenesis,which has provide more thoughts and direction for the occurrence and treatment of lung cancer.

AIM:To study the effects of isoproterenol (Iso) on cardiac fibrosis and related cytokines in rats. METHODS:20 SD rats were divided into control and Iso group in random, with 10 rats each group. After injected Iso, cardiac histology was observed and cardiac function, collagen volume fraction (CVF), content of collagen protein and the concentrations of Angiotensin Ⅱ (Angll) and nitrogen monoxide (NO) were measured in the two groups. RESULTS:Compared with control group, microscopic examination showed that fibrosis increased in Iso group. Iso did decrease cardiac function and NO levels and increase CVF, content of collagen protein and AngII levels. Left ventricular pressure maximal rate of rise (+ dp/dt_(max)) and left ventricular systolic pressure (LVSP) were not significantly different from that of control group. CONCLUSIONS:Iso can induce myocardial fibrosis and change levels of related cytokines in rats.

Five hundred and ninety-one papers retrieved from CAJD on performance assessment of electronic re-sources in domestic libraries from 2004 to 2013 were retrospectively analyzed by biliometrics, which showed that these papers focused on the description of projects,methods of assessment,and establishment of assessment indexes. The following problems were pointed out, such as weakness in research strength, tendency to stress theories, inopera-tive methods used in studies, and single-valued achievements, with suggestions put forward for their solution such as intensifying cooperation and focusing on practice in future studies.