1.Effects of bone marrow mononuclear cell implantation induced by 5-azacytidine on cardiac function
Hongxin XU ; Gengshan LI ; Hong JIANG
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(02):-
Objective: Recent studies have shown that cell implantation can replace infarcted myocardium to improve cardiac performance. The present study was designed to investigate the effects of autologous bone marrow mononuclear cell (BM-MNC) implantation into myocardium bordering the infarction with or without induced by 5-azacytidine on cardiac function after acute myocardial infarction (AMI) in rabbits. Methods: AMI was replicated by ligating the left anterior descending coronary artery. Rabbits were randomly divided into three groups: (1) BM -MNC induced by 5-azacytidine implantation (n=7), (2) BM-MNC implantation alo ne (n=7), and (3) AMI control (n=7). In addition, sham-operated (n=5) rabbit s were randomly selected to serve as non-infarction control. Animals for cell im p lantation were received intramyocardial injection of autologous BM-MNC in myoca rdium bordering the infarction, and echocardiography and hemodynamic studies wer e performed to evaluate cardiac function following 28 days of implantation. Results: Compared with the sham-operated group, the left ventricle (LV) end diastolic pressure (LVEDP) was significantly increased (P0.05). Conclusion: BM-MNC induced by 5-azacytidine implantation into myocardium bordering the infarction can significantly improve impaired cardiac function associated with LV remodeling after AMI, however such improvement is not further promoted compared with that in BM-MNC implantation group alone.
2.Protective effects of trimetazidine on bone marrow mesenchymal stem cells viability in an ex vivo model of hypoxia and in vivo model of locally myocardial ischemia.
Hongxin, XU ; Gangyan, ZHU ; Yihao, TIAN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2012;32(1):36-41
Bone marrow mesenchymal stem cells (MSCs) have shown potential for cardiac repair following myocardial injury, but this approach is limited by their poor viability after transplantation. The present study was to investigate whether trimetazidine (TMZ) could improve survival of MSCs in an ex vitro model of hypoxia, as well as survival, differentiation, and subsequent activities of transplanted MSCs in rat hearts with acute myocardial infarction (AMI). MSCs at passage 3 were examined for their viability and apoptosis under a transmission electron microscope, and by using flow cytometry following culture in serum-free medium and exposure to hypoxia (5% CO(2), 95% N(2)) for 12 h with or without TMZ. Thirty Wistar rats were divided into 3 groups (n=10 each group), including group I (AMI control), group II (MSCs transplantation alone), and group III (TMZ+MSCs). Rat MSCs (4×10(7)) were injected into peri-infarct myocardium (MSCs group and TMZ+MSCs group) 30 min after coronary artery ligation. The rats in TMZ+MSCs group were additionally fed on TMZ (2.08 mg·kg(-1)·day(-1)) from day 3 before AMI to day 28 after AMI. Cardiac structure and function were assessed by echocardiography at 28th day after transplantation. Blood samples were collected before the start of TMZ therapy (baseline), and 24 and 48 h after AMI, and inflammatory cytokines (CRP, TNF-α) were measured. Then the survival and differentiation of transplanted cells in vivo were detected by immunofluorescent staining. The cellular apoptosis in the peri-infarct region was detected by using TUNEL assay. Furthermore, apoptosis-related proteins (Bcl-2, Bax) within the post-infarcted myocardium were detected by using Western blotting. In hypoxic culture, the TMZ-treated MSCs displayed a two-fold decrease in apoptosis under serum-free medium and hypoxia environment. In vivo, cardiac infarct size was significantly reduced, and cardiac function significantly improved in MSCs and TMZ+MSCs groups as compared with those in the AMI control group. Combined treatment of TMZ with MSCs implantation demonstrated further decreased MSCs apoptosis, further increased MSCs viability, further decreased infarct size, and further improved cardiac function as compared with MSCs alone. The baseline levels of inflammatory cytokines (CRP, TNF-α) had no significant difference among the groups. In contrast, all parameters at 24 h were lower in TMZ+MSCs group than those in MSCs group. Furthermore, Western blotting indicated that the expression of anti-apoptotic protein Bcl-2 was up-regulated, while the pro-apoptotic protein Bax was down-regulated in the TMZ+MSCs group, compared with that in the MSCs group. It is suggested that implantation of MSCs combined with TMZ treatment is superior to MSCs monotherapy for MSCs viability and cardiac function recovery.
3.Analysis of clinical and pathological features of familial gastric cancer
Hongxin NIU ; Zhongfa XU ; Huiyong SHI ; Lili WANG
Chinese Journal of Current Advances in General Surgery 2009;0(11):-
Objective:To explore the clinical and pathological features of familial gastric cancer, and to get the early discovery and early treatment of it.Methods:Two kindreds of familial gastric cancer were followed up and their clinical and pathological features were analyzed.Results:Six patients with gastric cancer were found in the 2 kindreds.Autosomal dominant inheritance was observed in these cases.clinical and pathological features of familial gastric cancer were showed according to the document analysis:early onset;poor prognosis;patients suffer simultaneous or metachronous carcinoma;CDH1 germline mutation carriers had higher penetrance;pathologically, tumors are mostly diffuse infiltrative type with lower differentiated degree and earlier metastasis to lymphnodes;in one kindred,the sites of the lesions were relatively consistent.Conclusion:Familial gastric cancer has particular clinical and pathological features:early onset;poor prognosis;patients suffer simultaneous or metachronous carcinoma;CDH1 germline mutation carriers have higher penetrance; pathologically,tumors are mostly diffuse infiltrative type with lower differentiated degree and earlier metastasis to lymphnodes.
4.Study on Nuclear Factor-?B Activation in Skin Lesions of Systemic Lupus Eryt hematosus
Chunhong FANG ; Hong LIANG ; Jianjun LI ; Hongxin XU ; Layuan XIONG
Chinese Journal of Dermatology 1994;0(06):-
Objective To examine whether activation of nuclear factor-?B (NF-?B) exists in skin lesions of patients with systemic lupus erythematosus (SLE ) and its association with disease activity. Methods The skin lesions were inves tigated histopathologically in patients with SLE, and NF-?B activation was ass essed by immunohistochemical analysis semi quantitatively. Results Expression o f NF-?B was found on skin lesions in 14 of 15 patients with SLE, including 8 s trong positive (), 3 moderate positive (), and 3 mild positive (+). Brown-coloured particles were mainly distributed in keratinocytes, especially in prick le cells and granular layer cells, as well as in mononuclear cells of dermis. Th ere was no correlation between NF-?B activation and disease activity. However, NF-?B was not detected in skin lesions of all patients with non-SLE and heal thy controls. Conclusions NF-?B activation may be associated with the developm ent of skin lesions in patients with SLE,and not with disease activity.
5.The follow-up study of image guided 125I seed permanent brachytherapy for the treatment of unresectable pancreatic cancer
Hongxin NIU ; Xikun ZHANG ; Zhongfa XU ; Zhao ZHOU
Chinese Journal of General Surgery 2015;30(6):432-435
Objective To explore the clinical efficacy of image-guided 125I radioactive seed interstitial implantation therapy for unresectable pancreatic cancer.Methods 25 patients with unresectable pancreatic cancer evaluated by retrospective follow-up were enrolled in this study,13 patients received radioactive seeds implantation while 12 patients were given non-surgical treatment.We observe and compare the clinical benefits,objective curative effect,complications,adverse reaction,survival between the two groups of patients.Results Compared with the non-surgical treatment group,the clinical benefit rate in the radiotherapy seed implantation group was 92% (12/13) while that of the non-surgical group was 42% (5/12),the difference was of statistically significance.The numbers of cases evaluated as effective were 6 (46%) and 4 (33 %) respectively,the difference was not statistical significant (x2 =0.427,P > 0.05);The radioactive seed implantation group had no serious postoperative complications;3 cases who received subsequent chemotherapy in radioactive seed implantation group(23%,3/13) and 3 cases in non-surgical treatment group(25%,3/12)suffered from serious adverse reactions,the difference was of no statistical significance(x2 =0.013,P >0.05);Comparing the survival rate between the two groups,x2 =0.001,P =0.969,the difference was of no statistical significance.Conclusions The therapy of 125I radioactive seed implantation for unresectable pancreatic cancer significantly relieves cancer caused pain and improves quality of life.
6.Vascular imaging research of patients with cervical vertigo
Hongxin ZHANG ; Jun LIU ; Liang XU ; Caixian HAO
Journal of Practical Radiology 2016;32(3):419-422
Objective To analyze the imaging characteristics and to evaluate the application value of color doppler ultrasonography (CDUS)combined with CT angiography (CTA)and contrast-enhanced magnetic resonance angiography (CE-MRA)in patients with cervical vertigo.Methods 62 patients diagnosed with cervical vertigo clinically were enrolled.Neck CDUS and neck CTA were per-formed on 39 patients.Neck CDUS and neck CE-MRA were performed on 23 patients.Neck CDUS and CTA/MRA were performed on 30 normal volunteers,which were chosen as control group.Neck CDUS and neck CTA were performed on 18 normal volunteers. Neck CDUS and neck CE-MRA were performed on 12 normal volunteers.Hemodynamics and morphology were evaluated and com-pared between the two groups.Results In aspect of morphology:The incidence of vertebral artery (VA)stenosis (46.77%)and VA variation (29.03%)in cervical vertigo group were higher than VA stenosis (23.33%)and VA variation (6.67%)in control group with significant difference (all P <0.05).The incidence of tortuous VA showed no statistics difference between cervical vertigo group (1 1.29%)and control group (13.33%)(P >0.05).In aspect of hemodynamics:The decline incidence of peak systolic velocity de-tected by CDUS in cervical vertigo group (66.13%)was higher than that in control group (10.00%)with statistically significant difference (P <0.05).The peak systolic velocity decline incidence of VA stenosis (86.21% )and VA variation (72.22%)were high-er than that of VA tortuous (28.57%)and VA normal (12.50%)patients in cervical vertigo group.Conclusion The application of CDUS combined with CTA or CE-MRA could provide valuable diagnostic and therapeutic information for cervical vertigo in the as-pects of change in vascular morphology and cerebral hemodynamics,which could further provide objective basis for clinical diagnosis and treatment.
7.Clinical effect of Jingyuankang capsule on the level of peripheral leukocytes in patients with HIV/AIDS: a review of randomized control study on 116 cases
Shiqing JIANG ; Hongxin SUN ; Yingmin XU ; Junwen PEI ; Hongling WANG ; Jianguang ZHU
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
Objective: To observe the therapeutic effect of Jingyuankang capsule on peripheral leukopenia in patients with HIV/AIDS.Methods: The methods of randomized,double blind,double analogue clinical trials were used for two groups: 58 cases treated with Jingyuankang capsule,Likejun tablet analogue and HAART therapy;58 cases treated with Likejun tablets,Jingyuankang capsule analogue and HAART therapy.Both groups had systematic administration of 6 weeks.The peripheral blood cells were examined to observe the therapeutic effect of Jingyuankang capsule on peripheral leukopenia.Results: Jingyuankang capsule had a good therapeutic effect in treating peripheral leukopenia.It signifi cantly increased the numbers of leukocytes and granulocytes.As the time of administration,it could promote the growth of leukocyte more obviously.It was clinical safe and effective.No toxic and side effect was found in the process of treatment and later follow-up visit.Conclusion: Jingyuankang capsule can effectively treat peripheral leukopenia in patients with HIV/AIDS.
8.The effect of Rehmannia glutinosa oligosaccharides on the damage induced by glutamate in hippocampal neurons
Jialin SHI ; Jing YANG ; Xinli XU ; Hongxin WANG ; Ying JIN ; Chunna LIU
Chinese Pharmacological Bulletin 1987;0(03):-
Aim To investigate the effects of Rehmannia glutinosa oligosaccharides(ROS)on the damage induced by glutamate in hippocampal neurons.Methods The neurons isolated from hippocampus in new born SD rats were cultured for 7~9 days,which were specifically stained with NSE and then randomly divided into four groups:(Ⅰ)Normal cultures(control);(Ⅱ)ROS control cultures;(Ⅲ)Glutamate-exposed control cultures;(Ⅳ)Glutamate-exposed cultures pretreated with ROS.The neurons morphology was observed under inverted microscope;cell viability was assayed by MTT staining;LDH release was detected with chromatometry and flow cytometric analysis for identification and quantification of cell apoptosis.Results Compared with normal group,after exposure of glutamate for 24 h,the viability of neurons was decreased,LDH release and cell apoptosis were increased(P
9.Comparison of different conditions inducing embryonic stem cells in vitro to differentiate into cardiomyocytes
Haiyan QIAN ; Gengshan LI ; Hongxin XU ; Jingjun L ; Wen JIANG ; Jing WANG
Chinese Journal of Pathophysiology 2000;0(12):-
AIM: To evaluate the different conditions inducing mouse embryonic stem cells (ESC) in vitro to differentiate into cardiomyocytes. METHODS: BRL conditioned medium was used to promote the growth of ESC and maintain them in an undifferentiated state. During the inducing process, retinoic acid (RA), DMSO, activin-A and TGF-? 1 were used as inducing reagents, and made up six kinds of differentiating medium. Then a three-step method inducing ESC cultured in hanging drops, in suspension and in plating was used to induce the differentiation of ESC. RESULTS: ESC were induced in vitro to differentiate into cardiomyocytes. Of all groups, the highest differentiating rate was observed in the group induced by activin-A (20 ?g/L) and TGF-? 1 (2 ?g/L). CONCLUSION: The inducing conditions including activin-A (20 ?g/L) and TGF-? 1 (2 ?g/L) is very valuable in inducing ESC differentiation into cardiomyocytes. [
10.Effect of valsartan on atherosclerosis in the cholesterol-fed rabbits
Hongxin XU ; Jianjun LI ; Gengshan LI ; Jing WANG ; Xiaoyan LI ; Xuejun JIANG
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To investigate whether valsartan inhibits the development of atherosclerosis in cholesterol-fed rabbits and its possible mechanism. METHODS: Male rabbits were fed either: (1) normal rabbit chow for 16 weeks; (2) 1.5% cholesterol diet for 16 weeks; or (3) 1.5%cholesterol diet for 16 weeks supplemented by valsartan(3 mg?kg -1?d -1) for the last 4 weeks. After 16 weeks, the arteries were harvested for histomorphometry and immunohistochemistry. RESULTS: Rabbits fed with cholesterol-rich diet showed higher serum lipids levels(P