Objective:To investigate the effects of HSP90 inhibitors Geldanamycin(GA) on invasion and motility potential in human breast cancer cell.Methods: The effects of HSP90 inhibitor GA on the adhesion?invasion and metastasis of human breast cancer line MDA-MB-231 and MDA-MB-435s in vitro were explored by MTT colorimetric and transwell chamber.Results: Treatd with GA,the adhesion? invasion and motility abilities of the MDA-MB-231 and MDA-MB-435s cells were decreased significantly as compared with the control guoup,(P

Objective:To investigate the expression and effect on invasion and metastasis of HSP90 in human breast cancer cell line ZR-75-30 in vitro and the mechanism of anti-invasion.Methods:The protein expression level of HSP90 in ZR-75-30 before and after treatment with HSP90 inhibitor Geldanamycin was detected by Western-blotting;The effects of GA on the proliferation,invasion and motility of human breast cancer cell line ZR-75-30 in vitro were explored respectively by MTT colorimetric assay and transwell chamber.Results:After treatment with GA the protein expression level of HSP90 in ZR-75-30 was decreased,and the proliferation of ZR-75-30 cells was obviously inhibited in a dose and time-dependent manner.Compared with the control guoup,the adhesion,invasion and motility abilities of the ZR-75-30 cells were decreased significantly(P

OBJECTIVE:To investigate the protective effects of adjunctive general anesthesia of dexmedetomidine on cardiac and cerebral ischemia-perfusion injury. METHODS:50 patients,ASA physical status Ⅰ-Ⅱ,undergoing selective tracheal intuba-tion,were randomly divided into dexmedetomidine group (group D) and control group (group C) with 25 cases in each group. The patients in group D received a loading dose of 1 μg/kg of dexmedetomidine by infusion pump before anaesthesia,10 min lat-er by a maintenance dose of 0.5 μg/(kg·h)till the end of surgery;whereas patients in group C received normal saline and same induction. HR,SBP and DBP of 2 groups were monitored at the beginning of operation,10 min after incision,30 min after inci-sion,at the end of operation. Blood samples of vena jugularis interna were taken to determine the plasma levels of cardiac tropo-ninⅠ(cTnⅠ) ,adrenaline and norepinephrine before anesthesia induction (T0),at the end of surgery (T1),6 h after surgery (T2),12 h after surgery(T3),24 h after surgery(T4),48 h after surgery(T5). RESULTS:There was no statistical significance in the levels of HR,SBP and DBP in group D between during operation and at the beginning of operation(P>0.05);those in-dex were significantly lower than those of group C at corresponding time points,with statistical significance(P<0.05). The lev-els of cTnⅠ in group D at T2-T5 were significantly lower than in group C,with statistical significance(P<0.05). The levels of adrenaline and norepinephrine at T1-T5 were significantly lower than group C,with statistical significance (P<0.05). CONCLU-SIONS:Adjunctive general anesthesia of dexmedetomidline can improve cardiac and cerebral ischemia-perfusion injury to certain extent.

Objective To investigate the correlation between the HSP90 expression and invasion and metastasis potential of human breast cancer cell line. Methods The mRNA expression level of HSP90 was detected by RT-PCR. The protein expression level of HSP90 was measured by Western blotting. The effects of HSP90 inhibitor Geldanamycin (GA) on the invasion and motility abilities of human breast cancer line MDA-MB-231 and MDA-MB-435s in vitro were explored by transwell chamber. Results The mRNA expression level of HSP90? in MDA-MB-435s cell was higher than that of MDA-MB-231 cell (P0.05) between the two cell lines. The protein expression level of HSP90 in MDA-MB-435s cell was higher than that of MDA-MB-231 cell (P0.05). After treated with GA, the invasive and metastatic potential in human breast cancer cell MDA-MB-435s and MDA-MB-231 was suppressed significantly (P

Objective:To evaluate the shaping effects of multi-taper ProTaper combined with nickel-titanium(Ni-Ti) K file in simulated S-shaped canals. Methods:Thirty simulated S-shaped resinous canals were randomly divided into three groups and prepared with hand ProTaper, hand Ni-Ti K file, hand ProTaper combined with Ni-Ti K file respectively. The shaping effects were assessed by the amount of material removed from the inner and outer wall of canal as well as the width of canal after instrumentation. Results: The shaping files of ProTaper removed the least material in amount of materials from the same apical dimension. The finishing files of ProTaper removed the most amount of material at the inner side of curvature and outer side of apex. ProTaper combined with Ni-Ti K file removed the least material at the above parts and the widths of canals prepared were gradually decreased from the orifice to the apex. Conclusion:ProTaper combined with Ni-Ti K file exhibited good shaping effects in simulated S-shaped canals.

Background The enhance of differentiation of orbital preadipocyte is a main factor for pathogenesis of thyroid associated ophthalmopathy(TAO).Experiment has proved that orbital preadipocyte can differentiate into mature adipocyte expressing resistin,and resistin is a adipocyte factor with biological activity.Objective The present study is to investigate the effects of recombinant human resistin on differentiation of orbital preadipocytes derived from TAO patients orbital tissues.Methods Orbital adipose tissue was obtained from 10 eyes with TAO during the orbital decompression surgery.Orbital adipose tissue of the normal group was obtained from 5 removed eyes during the surgery due to different reasons.Preadipocytes were isolated and cultured using expand culture method.The 2-5 generations of preadipocytes were identified by oil red O staining under the light microscope.0,10,25,50,100ng/mL of recombinant human resistin were added into medium in the first day after differentiation of cells.The expression of PPARγ mRNA in differentiated adipocytes were detected by reverse trancripaton PCR(RT PCR).Results The differentiation of preadipocyte presented the similar procedure and outcome between the TAO group and normal group.Differentiated adipocyte was stained into the jacinth by oil red O.The expression of PPARγ mRNA in differentiated cells was gradually declined with the increase of concentration of recombinant human resistin (F=241.341,P＜0.01).A dose dependent inhibitory effect was detected in the expression of PPARγ mRNA.The weakest expression of PPARγ mRNA in differentiated cells was 100ng/mL resistin group.Conclusion The recombinant human resistin has an inhibitory effect on the differentiation of orbital preadipocytes by reducing the expression of adipose related genes.Resistin is probably a new research approach to treatment of TAO.

Total hip replacement (THR) is replacing the prosthesis stem similar to human bone that takes advantage of the material with both good mechanical properties and biocompatibility to the damaged articular surface. Thus it can not only alleviate or even eliminate the pain but also effectively maintain the joint stability and freedom and restore its normal performance. Finite element analysis was used in this study to establish a 3D model of artificial hip stem, and explore its fatigue properties of different materials to ensure the safety and reliability. The calculating obtained two results of different metal hip prosthesis, including lifetime and deformation. The minimum service life of titanium prosthesis reaches 568 million times, which satisfies ISO standards, while the stainless steel does not suit to be a prosthesis material.
Arthroplasty, Replacement, Hip ; Finite Element Analysis ; Hip Joint ; Hip Prosthesis ; Humans ; Materials Testing ; Models, Anatomic ; Prosthesis Failure ; Reproducibility of Results ; Stainless Steel ; Titanium

0.05 ). The assessments of the 4 examiners were uniform(?=0.57~0.65).Conclusion:Radiograph can provide essential evidence for the clinical diagnosis of the vertical root fracture in mandibular first molar.

OBJECTIVE:To study the percutaneous permeability of methoxypsoralen liposomal gel METHODS:The preparations were applied on the rat skin in vitro and in vivo with common gel and Mingbailing tincture as control,and the drug concentrations in the receiver,skin,blood and other organs were determined by RP-HPLC RESULTS:In vitro experiment,liposomal gel showed the lowest percutaneous permeation rate and the highest retention in skin In vivo studies illuminated that the skin depositions of drug encapsulated in liposome was(4 37?0 91) and (3 36?0 58) times those of the common gel and tincture respectively CONCLUSION:Methoxypsoralen liposomal gel showed an evident targeting effect on skin

A large-area graphene platform electrode (GPE) was fabricated by transferring the chemical vapor deposition (CVD) graphene grown on Cu foils to polyethylene terephthalate (PET) substrates with the aid of polymethyl-methacrylate (PMMA).Then cobalt hexacyanoferrate (CoHCF) was electrodeposited on the GPE by cyclic voltammetry to obtain a CoHCF/GPE.The morphology and electrochemical properties of this electrode were studied by scanning electron microscope (SEM), electrochemical impedance spectroscopy (EIS) and the electrochemical measurements.Results showed that the electrode had favorable sensing effect toward H2O2.On the basis of this, a new nonenzyme H2O2 sensor was constructed.Under the optimal experimental conditions, the proposed sensor had a quick response to the addition of H2O2 (about 2 s) with a wide linear rang (5 × 10-3-1.2 mmol/L) and a low detection limit (7.1 nmol/L).This sensor was easy to fabricate and showed excellent stability and anti-interference ability.