Objective To investigate the surface sterilization and modification of medical poly(tetrafluoroethylene)(PTFE) by remote argon plasma.Methods Carrier quantitative bactericidal test was used to examine the germicidal effect(GE) of the E.coli on the surface of medical PTFE film and surface structure,performances of sterilized PTFE were characterized by the water contact angle,mass loss and X-ray photoelectron spectroscopy(XPS).Results Both remote and conventional argon plasmas could sterilize effectively(GE≥3.769) in the conditions of plasma RF power 100 W,exposure time 120 s and argon flux 20 cm3/min.Remote argon plasma sterilization made the PTFE surface have higher hydrophilicity(?=58.5?) than the conventional argon plasma sterilization(?=70.5?) and did not cause remarkable degradation and damages in terms of the mass loss(mass loss was 11.8%).Remote argon plasma sterilization could enhance interaction reactions with argon radicals relative to those with electron and argon ions,thereby contributing more effectively to defluorination(F/C=2.24) from the PTFE than the conventional argon plasma sterilization(F/C=2.49) and more effectively to the formation of oxygen functionalities on the PTFE surface.Conclusion Remote argon plasma can inactivate E.coli effectively and at the same time bring better surface modification of PTFE.

Objective To observe the clinical curative effect of Tanreqing injection of antibiotics in the treatment of pulmonary infection in the elderly.Methods 120 cases of elderly patients with pulmonary infection from June 2015 to June 2016 in our hospital were selected, randomly divided into observation group and control group,60 cases in each group, the observation group treated with Tanreqing injection and oxygen, anti-inflammatory, expectorant therapy,the control group treated only with oxygen, anti-inflammatory, expectorant therapy, the clinical symptoms and therapeutic effects were compared between the two groups.Results After seven days of treatment, PaO2 , SaO2 and pH of the observation group were higher than those of the control group (P<0.05), the levels of PaCO2, CRP, IL-6, IL-13, LTB4 and WBC were lower than those in the control group (P<0.05); After 14 days of treatment, CRP, IL-6, IL-13 and LTB4 in the observation group were lower than those in the control group (P<0.05); The total effective rate was 75.00%in the observation group and 58.33%in the control group after 14 days of treatment.There was significant difference between the two groups (P<0.05).Conclusion Tanreqing injection combined with western medicine antibiotic treatment of elderly patients with pulmonary infection was significant,can significantly improve the clinical symptoms of patients and improve clinical efficacy .

Objective To explore the coping styles and family environment in patients with depression.Methods 90 patients with depression were evaluated with CSQ and FES-CV.Results There were significant differences in the family environment between depression group and normal control group(P＜0.01).The factor scores of seeking-help and solving question in depression group positively correlated with the factor scores of family intimacy and success(r=0.29,0.32,0.27,0.31,P＜0.05).The factor score of self-accusation in depression group negatively correlated with the factor score of affection expression(r=-0.27,P＜0.05).Conclusion The coping styles are associated to family environment in patients with depression.

Objective To discriminate the species and individual identification with mitochondrial DNA (mtD-NA) sequencing in forensic medicine practice. Methods The multiplex PCR of mtDNA loop - D high - variation region and cytochrome- b region were investigated. The PCR products were detected with silver- stain method,followed by analysis of the PCR products with fluorescence sequence technique. Results The presence of two bands (358bp,279bp ) indicated the samples were from human, while only one band of 358bp indicated nonhuman origin. The part of mitochondrial DNA loop - D high - variation region (15997 ~ 16236) from 131 unrelated individuals of Guangdong population were sequenced. In all of these samples there were 69 nucleotide variations and 67 haplo-types.There was 2.679 mutation sites on average per person. The polymorphism was 97.92% . Conclusion The methods described here are reliable and very useful in species and personal identification of degraded samples.

Objective: To study the effects of compound transdermal enhancers on the transdermal kinetics of Baicalin. Methods: The uniform design was used for the selection of transdermal enhancers for the baicalin from various proportion of azone, propylene glycol and oleic acid. Results: The transdermal constant of baicalin is maxim when the proportion of is 6%,10%, and 0%, prespectively. Conclusion: The effects of compound transdermal enhancers is better than that of single one.

ObjectiveTo investigate the related factors of the occurrence of nosocomial infection and nursing management in operating room for general surgery patients,and provide a scientific basis to reduce the incidence of nosocomial infection of the operating room. Methods304 patients with nosocomial infection from August 2009 to August 2010 in our hospital for surgery were set as the control group.151 cases from September 2010 to October 2011 were named as the intervention group.All cases were analyzed retrospectively.The nursing factors which might affect nosocomial infection in operation room were analyzed by univariate and multivariate logistic regression analysis. ResultsBy univariate analysis,eight operation room nursing factors related to nosocomial infection were screened out:the type of incision,operation time,the following surgery or not,into ICU time,preoperative use of antibiotics,visitors of operations or not,invasive operation.5 highly correlated factors were screened out by multi-factor logistic regression analysis:admission to the ICU time,incision type,operation time,preoperative use of antibiotics,then following surgery.The infection rate and prolapse rate of the control group were significantly higher than those of the intervention group. ConclusionsIn nursing work of the operation room,we can take a series of nursing management interventions to reduce the incidence of nosocomial infection of general surgery.

Objective To investigate the relationship between the serum levels of vascular endothelial growth factor(VEGF) and severity and prognosis by evaluating its changes in severe sepsis patients. Method Us-ing control study design, a total of 29 severe sepsis patients who admired in ICU department of Second Hospital of Tianjin Medical University from July 2006 to November 2007 were enrolled. The patients were divided into survival group (n=16) and death group (n=13) according to the clinical outcomes at 28 days after onset.A total of 31 healthy persons were enrolled into the control group. Clinical and laboratory data including blood routine test,blood-gas analysis,blood chemistry,C-reactive protein,lactic acid were collected on the first,third and 7th day after on-set,respectively. APACHE Ⅱ score were calculated. VEGF levels were determined using ELISA method. Quantita-tive data were analyzed by Ftest. Results The VEGFlevels ofthe control groupwere (78.77±8.15) pg/mL, the VEGF levels of the survival group on the first,third and 7th day were (210.47±59.40) pg/mL, (161.79 ±32.58) pg/mL and (85.33±12.13) pg/mL, respectively. The peak value of VEGF levels appeared on the first day, Then,it decreased with the progression of the disease. The VEGF levels did not differ significantly between the control group and survival group on the 7th day (P>0.05). The VEGF levels in the death group on the first, third and 7th day were (324.12±44.35) pg/mL,(185.40±30.92) pg/mLand (273.32±55.23) pg/mL, respectively. The peak value of VEGF levels in the death group also appeared on the first day, but it did not de-crease significandy on the 7 th day as compared that on theist day. The value of VEGF levels on the 7 th day were significantly higher than that of the control group (P<0.01 ). The levels of VEGF were positive correlated with APACHEⅡ score(rs = 0.510,P<0.01), both VEGF levels and APACHEⅡ score were risk factors for the clinical outcomes of these patients. Conclusions The serum levels of VEGF are elevated at early stage in severe sepsis patients. The VEGF levels, which might be a potential prognositic factor for sepsis patients are significantly correlated with APACHE Ⅱ score.

Objective The national essential medicine select system is the core of Chinese medicine policy.How to select drugs fundamental for eyes scientifically is an important ring in establishing the Chinese essential drug system.The present study attempted to compare the National Essential Medicines List and National Essential Insurance List of China with that of the WHO in order to provide reasonable evidence for the adjustment of a new National Essential Medicines List of China. MethodsThe WHO Essential Medicine List (15th edition,Version in 2007),National Essential Medicine List (Version in 2009,China),2009 National Essential Medicine List Chemical Medicine Name (Version in 2009) and National Essential Insurance List of China (Version in 2004) were reviewed.The similarity and difference in the category and number of drugs in the National Essential Medicines List and National Essential Insurance List between China and WHO were compared and analyzed.A descriptive method was adopted to analyze the sorts and numbers of eye drugs in the lists mentioned above.The analysis of drug price was based on the summary sheet from Zhejiang Province. ResultsLittle difference was found in the numbers of eye drugs between the National Essential Medicines List of China and WHO Essential Medicines List.Differences in the sorts of eye drugs were observed in the lists,especially between the National Essential Medicines List of China and WHO Essential Medicines List.Except for levofloxacin,all of the drugs in the National Essential Medicine List of China were included in the National Essential Insurance List of China.ConclusionThe selecting principle and renewing procedure of the National Essential Medicines List and National Essential Insurance List of China should be further improved in China based on the list from WHO.More attention should be paid to the standard,dosage form,affordability,maneuverability etc.during the selecting procedure of eye essential drugs.

Background The enhance of differentiation of orbital preadipocyte is a main factor for pathogenesis of thyroid associated ophthalmopathy(TAO).Experiment has proved that orbital preadipocyte can differentiate into mature adipocyte expressing resistin,and resistin is a adipocyte factor with biological activity.Objective The present study is to investigate the effects of recombinant human resistin on differentiation of orbital preadipocytes derived from TAO patients orbital tissues.Methods Orbital adipose tissue was obtained from 10 eyes with TAO during the orbital decompression surgery.Orbital adipose tissue of the normal group was obtained from 5 removed eyes during the surgery due to different reasons.Preadipocytes were isolated and cultured using expand culture method.The 2-5 generations of preadipocytes were identified by oil red O staining under the light microscope.0,10,25,50,100ng/mL of recombinant human resistin were added into medium in the first day after differentiation of cells.The expression of PPARγ mRNA in differentiated adipocytes were detected by reverse trancripaton PCR(RT PCR).Results The differentiation of preadipocyte presented the similar procedure and outcome between the TAO group and normal group.Differentiated adipocyte was stained into the jacinth by oil red O.The expression of PPARγ mRNA in differentiated cells was gradually declined with the increase of concentration of recombinant human resistin (F=241.341,P＜0.01).A dose dependent inhibitory effect was detected in the expression of PPARγ mRNA.The weakest expression of PPARγ mRNA in differentiated cells was 100ng/mL resistin group.Conclusion The recombinant human resistin has an inhibitory effect on the differentiation of orbital preadipocytes by reducing the expression of adipose related genes.Resistin is probably a new research approach to treatment of TAO.

Objective To observe clinical curative effect of acupuncture combined with western medicine in treating acquired paralytic strabismus.Methods Randomly divided 60 patients with acquired paralytic strabismus into a treatment group and a control group,with 30 cases in each group.The control group accepted compound anisodine injection for subcutaneous injection beside superficial temporal artery and oral administration of neurotrophic drugs.The treatment group accepted acupuncture treatment in addition to the treatment of the control group.Both groups were treated for 4 weeks.Before and after treatment,measured the ocular symptom scores,including eye position deviation,eyeball movement obstacle,diplopia,the first squint angle being greater than the second,compensatory head posture and evaluated the clinical curative effect.Results The clinical curative rate in the treatment group was 40.0％ and the total effective rate was 93.4％,which in the control group were 10.0％ and 66.7％ respectively.The total effective rate and curative rate in the treatment group were significantly higher than those in the control group (P＜ 0.05).After treatment,the improvement in the total ocular symptom score and diplopia,deviation of eye position,eyeball movement disorder scores were superior to those in the control group (P ＜ 0.05).Conclusion Acupuncture combined with western medicine was superior to simple western medicine in treating acquired paralytic strabismus,and could significantly improve the eye symptom.