Objective To explore the coping styles and family environment in patients with depression.Methods 90 patients with depression were evaluated with CSQ and FES-CV.Results There were significant differences in the family environment between depression group and normal control group(P＜0.01).The factor scores of seeking-help and solving question in depression group positively correlated with the factor scores of family intimacy and success(r=0.29,0.32,0.27,0.31,P＜0.05).The factor score of self-accusation in depression group negatively correlated with the factor score of affection expression(r=-0.27,P＜0.05).Conclusion The coping styles are associated to family environment in patients with depression.

Objective To investigate the surface sterilization and modification of medical poly(tetrafluoroethylene)(PTFE) by remote argon plasma.Methods Carrier quantitative bactericidal test was used to examine the germicidal effect(GE) of the E.coli on the surface of medical PTFE film and surface structure,performances of sterilized PTFE were characterized by the water contact angle,mass loss and X-ray photoelectron spectroscopy(XPS).Results Both remote and conventional argon plasmas could sterilize effectively(GE≥3.769) in the conditions of plasma RF power 100 W,exposure time 120 s and argon flux 20 cm3/min.Remote argon plasma sterilization made the PTFE surface have higher hydrophilicity(?=58.5?) than the conventional argon plasma sterilization(?=70.5?) and did not cause remarkable degradation and damages in terms of the mass loss(mass loss was 11.8%).Remote argon plasma sterilization could enhance interaction reactions with argon radicals relative to those with electron and argon ions,thereby contributing more effectively to defluorination(F/C=2.24) from the PTFE than the conventional argon plasma sterilization(F/C=2.49) and more effectively to the formation of oxygen functionalities on the PTFE surface.Conclusion Remote argon plasma can inactivate E.coli effectively and at the same time bring better surface modification of PTFE.

Objective To observe the clinical curative effect of Tanreqing injection of antibiotics in the treatment of pulmonary infection in the elderly.Methods 120 cases of elderly patients with pulmonary infection from June 2015 to June 2016 in our hospital were selected, randomly divided into observation group and control group,60 cases in each group, the observation group treated with Tanreqing injection and oxygen, anti-inflammatory, expectorant therapy,the control group treated only with oxygen, anti-inflammatory, expectorant therapy, the clinical symptoms and therapeutic effects were compared between the two groups.Results After seven days of treatment, PaO2 , SaO2 and pH of the observation group were higher than those of the control group (P<0.05), the levels of PaCO2, CRP, IL-6, IL-13, LTB4 and WBC were lower than those in the control group (P<0.05); After 14 days of treatment, CRP, IL-6, IL-13 and LTB4 in the observation group were lower than those in the control group (P<0.05); The total effective rate was 75.00%in the observation group and 58.33%in the control group after 14 days of treatment.There was significant difference between the two groups (P<0.05).Conclusion Tanreqing injection combined with western medicine antibiotic treatment of elderly patients with pulmonary infection was significant,can significantly improve the clinical symptoms of patients and improve clinical efficacy .

Objective To discriminate the species and individual identification with mitochondrial DNA (mtD-NA) sequencing in forensic medicine practice. Methods The multiplex PCR of mtDNA loop - D high - variation region and cytochrome- b region were investigated. The PCR products were detected with silver- stain method,followed by analysis of the PCR products with fluorescence sequence technique. Results The presence of two bands (358bp,279bp ) indicated the samples were from human, while only one band of 358bp indicated nonhuman origin. The part of mitochondrial DNA loop - D high - variation region (15997 ~ 16236) from 131 unrelated individuals of Guangdong population were sequenced. In all of these samples there were 69 nucleotide variations and 67 haplo-types.There was 2.679 mutation sites on average per person. The polymorphism was 97.92% . Conclusion The methods described here are reliable and very useful in species and personal identification of degraded samples.

objective To detect MRSE and resistance in staphylococcus epidermidis isolates. Methods MRSE isolates were detected by cefoxitin disk test and susceptibilities were tested by agar dilution method. Results 79.7% of 128 isolates were MRSE strains with resistance to most antimicrobial agents, but those isolates were still susceptible to vancomycin and teicoplanin. Resistant rate in MRSE was higher than that in MSSE to most antimicrobial agents except penicillins, glycopeptides and macrolides. Conclusion MRSE with multi-resistance ale highly prevalent in staphylococcus epidermidis isolates and no isolate is resistant to glycopeptides.

Objective:To study intervenient action of Qixue Bingzhi Prescription on the patient of cervical atherosclerosis(AS). Methods:63 cases of cervical AS were randomly divided into a treatment group(n=33) and a control group(n=30), with 25 cases of same age ranges used as healthy group. The control group was treated with basic treatment of western medicine and the treatment group with the basic treatment plus Qixue Bingzhi Prescription for one month. Changes of blood lipid metabolism, interleukin levels, supersensitive C-reactive protein(Hs-CRP) content and the leukocyte adherence expression rate were observed. Results: The total cholesterol (TC), triglyceride(TG), AS index (TC/HDL-C), interleukin-6 (IL-6), interleukin-8 (IL-8) and Hs-CRP levels and lymphocytes and the neutrophilic granulocyte adhesion molecule expression rate (ICD11b and zCD11b) decreased significantly(P 0. 05), Conclusion: Qixue Bingzhi Prescription can effectively and safely intervene in blood lipid metabolism and inflammatory reaction in the patient of cervical AS, and it has prospect of development in preventing formation of AS and stabilizing plaque.

Objective:To study the diagnostic value about enhanced CT and gastroscopy for esophageal varices.Methods: Ninety eight patients with esophageal varices were selected as objects. All the patients underwent enhanced CT and gastroscopy, took detailed records of the test results, and analyzed CT scan at the portal vein and spleen vein values.Results: The results of enhanced CT detection and gastroscopy diagnosis were similar in liver cirrhosis combined with esophageal varices, liver cirrhosis combined with esophageal and gastric fundus varices, and liver cirrhosis complicated with gastric fundus varices. The diagnosis of enhanced CT is significant difference (F=33.06,F=20.22;P<0.05) in detection of portal vein value and spleen vein value for mild, moderate, and severe patients.Conclusion: The enhanced CT and gastroscopy for detecting of esophageal and gastric fundus has satisfactory value, and enhanced diagnostic CT for detecting severities of the esophageal and gastric fundus varices also has a important significance.

Objective The national essential medicine select system is the core of Chinese medicine policy.How to select drugs fundamental for eyes scientifically is an important ring in establishing the Chinese essential drug system.The present study attempted to compare the National Essential Medicines List and National Essential Insurance List of China with that of the WHO in order to provide reasonable evidence for the adjustment of a new National Essential Medicines List of China. MethodsThe WHO Essential Medicine List (15th edition,Version in 2007),National Essential Medicine List (Version in 2009,China),2009 National Essential Medicine List Chemical Medicine Name (Version in 2009) and National Essential Insurance List of China (Version in 2004) were reviewed.The similarity and difference in the category and number of drugs in the National Essential Medicines List and National Essential Insurance List between China and WHO were compared and analyzed.A descriptive method was adopted to analyze the sorts and numbers of eye drugs in the lists mentioned above.The analysis of drug price was based on the summary sheet from Zhejiang Province. ResultsLittle difference was found in the numbers of eye drugs between the National Essential Medicines List of China and WHO Essential Medicines List.Differences in the sorts of eye drugs were observed in the lists,especially between the National Essential Medicines List of China and WHO Essential Medicines List.Except for levofloxacin,all of the drugs in the National Essential Medicine List of China were included in the National Essential Insurance List of China.ConclusionThe selecting principle and renewing procedure of the National Essential Medicines List and National Essential Insurance List of China should be further improved in China based on the list from WHO.More attention should be paid to the standard,dosage form,affordability,maneuverability etc.during the selecting procedure of eye essential drugs.

Background The enhance of differentiation of orbital preadipocyte is a main factor for pathogenesis of thyroid associated ophthalmopathy(TAO).Experiment has proved that orbital preadipocyte can differentiate into mature adipocyte expressing resistin,and resistin is a adipocyte factor with biological activity.Objective The present study is to investigate the effects of recombinant human resistin on differentiation of orbital preadipocytes derived from TAO patients orbital tissues.Methods Orbital adipose tissue was obtained from 10 eyes with TAO during the orbital decompression surgery.Orbital adipose tissue of the normal group was obtained from 5 removed eyes during the surgery due to different reasons.Preadipocytes were isolated and cultured using expand culture method.The 2-5 generations of preadipocytes were identified by oil red O staining under the light microscope.0,10,25,50,100ng/mL of recombinant human resistin were added into medium in the first day after differentiation of cells.The expression of PPARγ mRNA in differentiated adipocytes were detected by reverse trancripaton PCR(RT PCR).Results The differentiation of preadipocyte presented the similar procedure and outcome between the TAO group and normal group.Differentiated adipocyte was stained into the jacinth by oil red O.The expression of PPARγ mRNA in differentiated cells was gradually declined with the increase of concentration of recombinant human resistin (F=241.341,P＜0.01).A dose dependent inhibitory effect was detected in the expression of PPARγ mRNA.The weakest expression of PPARγ mRNA in differentiated cells was 100ng/mL resistin group.Conclusion The recombinant human resistin has an inhibitory effect on the differentiation of orbital preadipocytes by reducing the expression of adipose related genes.Resistin is probably a new research approach to treatment of TAO.

Objective To explore the anxious condition and the influencing factors of chronic hepatitis B patients, and to take appropriate care measures. Methods Self-designed questionnaire for general information of patients, a comprehensive Zung self-rating depression scale(SDS)and Zung self-rating anxiety scale(SAS)were used to investigate 100 patients with chronic hepatitis B patients(the study group), and the results were compared with 80 normal persons(the control group). Results The SDS and SAS score of the study group was significantly higher than the control group. The negative emotion factors of the study group included social context, family, education, self- respect. Conclusions Patients with chronic hepatitis B has obvious negative mood, and are influenced by many kinds of factors. Strengthening the comprehensive psychological intervention can improve the negative emotional state, and promote rehabilitation of patients.