1.Expression of cellular FLICE-inhibitory protein in peripheral blood and lesion of patients with lichen planus
Chinese Journal of Microbiology and Immunology 2009;29(3):221-223
Objective To investigate the expression and distribution of cellular FLICE( Fas-assoelated death domain-like interleukin-I β-converting enzyme-like)-inhibitory protein (c-FLIP) in peripheral blood and lesions of lichen planus patients. Methods Peripheral blood and skin samples were obtained from 30 patients with lichen planns and 20 normal controls. Flow cytometry was used to detect intracellular c-Fl,lP in peripheral T and B lymphocytes, and immunohistochemistry was used to examine the expression of c-FLIP in lesionai tissue. Results Based on the pesitivity rate of c-FLIP, there was a significant increase in T iymphocytes in lichen planus compared with normal controls(6.32%±1. 17% vs 2.28%±0.54%, P < 0.05 ), while no significant difference was found in B lymphocytes between two groups (0.78% ±0. 16% vs 0.69% ± 0. 18%, P >0. 05 ). The expression intensity of c-FLIP in keratinocytes was also higher in lichen planusthanthatinnormalcontrols ( 89.73%± 5.24% vs 121.58% ±7.93% ,P < 0. 01 ). Conclusion c-FLIP is highly expressed in lesions and peripheral T lymphocytes of patients with lichen planus, which suggests the possible involvement of c-FLIP in the proliferation of T lymphocytes in lichen planns.
2.Percutaneous vertebroplasty, percutaneous kyphoplasty and expansive pedicle screw fixation for repairing primary osteoporotic thoracolumbar fractures
Ling WANG ; Hongxia ZHAO ; Qiang HUA
Chinese Journal of Tissue Engineering Research 2017;21(3):350-355
BACKGROUND:Percutaneous vertebroplasty, percutaneous kyphoplasty and expandable pedicle screw fixation can treat primary osteoporotic thoracolumbar fractures. The three methods have their own advantages and disadvantages. OBJECTIVE:To investigate the methods and clinical effects of primary osteoporotic thoracolumbar fractures. METHODS:Clinical data of 61 patients with primary osteoporotic thoracolumbar fractures were col ected and retrospectively analyzed. Perioperative preparation must be done. Al patients were treated by percutaneous vertebroplasty, percutaneous kyphoplasty and expansive pedicle screw fixation. We recorded Visual Analogue Scale (VAS) and the Oswestry Disability Index (ODI) before treatment, 3 months after treatment, as wel as sagittal index (SI) and Cobb angle of vertebral fracture before treatment, 3 days and 3 months after treatment. RESULTS AND CONCLUSION:(1) Al cases were fol owed up for 12-18 months. (2) There was no significant difference in VAS scores, ODI, SI and Cobb angle of vertebral fracture among the three groups of patients preoperatively. (3) At 3 months after treatment, there were significant differences in VAS scores and ODI in the three groups as compared with that preoperation (P<0.05). However, no significant difference in VAS and ODI was determined among intergroup comparison (P>0.05). (4) SI and Cobb angle of vertebral fracture were significantly increased;the difference was statistical y significant (P<0.05). The efficacy was similar between the percutaneous kyphoplasty and expansive pedicle screw fixation groups (P>0.05), and was better than the percutaneous vertebroplasty group (P<0.05). (5) Three kinds of treatment can effectively restore the vertebral height and intensity, relieve pain and stabilize the spine, and no significant vertebral compression was found in the short term. However, restoration of postoperative vertebral height was better in percutaneous kyphoplasty and expansive pedicle screw fixation groups than in the percutaneous vertebroplasty group. In view of their respective indications, advantages and disadvantages, the key point of raising therapeutic effect was to choose appropriate surgical procedures.
3.THE SCREENING OF THE CARBOXYPEPTIDASE PRODUCING STRAIN AND ITS PROPERTIES OF ENZYME PRODUCING
Hongxia FENG ; Zhaoxin LU ; Hua YOU ;
Microbiology 1992;0(01):-
One carboxypeptidase producing strain was obtained from 28 proteinase producing strains, by analyzing the products of peptides hydrolyzed by the enzyme of the strains According to the characteristics of the morpha and the colonies, the screened strain belongs to aspergillus genera The activity of its carboxypeptidase reached maximum at the 84th hour after fermentation
4.STUDY ON THE OPTIMUM FERMENTATION CONDITION FOR PRODUCTION OF PROTOPECTINASE BY ASPERGILLUS SP.
Hua YOU ; Zhaoxin LU ; Hongxia FENG ;
Microbiology 1992;0(01):-
Effects of carbon resource, nitrogen resource, metal irons and surface detergents on the production of pro topectinase by strain Aspergillus sp XZ 131 were studied The results showed that pectin substances were essential for the strain to produce protopectinase The enzyme activity reached to 300 U/mL, when(NH 4) 2SO 4 and(NH 4) 2HPO 4 were used as nitrogen resource Ca 2+ and Tween 20 were able to enhance the production of the enzyme The optimum composition of the medium was citrus peel powder 1g,(NH 4) 2SO 4 2g,CaCl 2 0 015g,Tween 20 0 2mL,KH 2PO 4 3 8g,K 2HPO 4?3H 2O 0 2g,H 2O 100mL,pH 6 5。
5.Random and control study comparing gemcitabine administered in fixed dose rate with a more standard infusion combined with oxaliplatin regimens in advanced biliary tract cancer patients
Yiyuan WAN ; Hongxia HUI ; Xiaowei WANG ; Hua LIU
Chinese Journal of Clinical Oncology 2014;(17):1098-1102
Objective:To investigate and compare the effects of oxaliplatin combined with gemcitabine administered in a fixed dose rate and that administered in a more standard infusion in advanced biliary tract cancer patients on chemotherapeutic efficacy, toxicities, and survival time. Methods:A total of 93 cancer patients were recruited from February 1, 2010 to December 12, 2012 in the First Hospital of Huai'an City Affiliated Nanjing Medical College. Those recruited were either newly diagnosed unresectable advanced biliary tract cancer patients by percutaneous liver biopsy or relapse or metastatic biliary tract cancer patients after operation. The patients were randomly divided into two groups. The first group was the study group in which the patients received chemotherapy with gemcitabine in a fixed dose rate of 10 mg/m2 per minute combined with oxaliplatin regimens. The other group was the control group in which the patients received chemotherapy with gemcitabine in a more standardized infusion within 30 min combined with oxaliplatin regimens. Each patient received four cycles, with at least two cycles of chemotherapy with GEMOX regimens every 21 d, with follow-up until death. The chemotherapeutic efficacy was evaluated. Toxicities were documented after each cycle. Results:The clinical characteristics of the two groups were well balanced before chemotherapy (P>0.05). The response rate (RR) and clinical benefit response of the study group were higher than those of the control group (P<0.05). The overall survival (OS) and time to progress (TTP) of the study group were longer than those of the control group (P<0.05). With respect to adverse events, the major side effect was hematological toxicity. The rate of gradeⅢ/Ⅳleucocytopenia and thrombocytopenia in the study group was remarkably higher than that in the control group (P<0.05). However, the rate of non-hematological toxicity was similar (P>0.05). Conclusion:Gemcitabine in a fixed dose rate combined with oxaliplatin regimens is a feasible and effective scheme in treating advanced biliary tract cancer patients. RR is higher and OS and TTP are longer under this scheme. Non-hematological toxicities are also well tolerated. However, hematological toxicity is distinguished. These results guide us to be prudent in utilizing this regimen. The investigation of the value of gemcitabine in a fixed dose rate combined with oxaliplatin in treating advanced biliary tract cancer patients is worth pursuing in future clinical trials.
6.Clinical analysis of 163 cases of pulmonary thromboembolism
Hua ZHANG ; Yiqiang CHEN ; Hongxia WEN ; Xiaoying ZOU ; Chen WANG
Chinese Journal of Practical Internal Medicine 2001;0(02):-
Objective To explore the role and significance of standardized scheme for diagnosis and treatment of pulmonary thromboembolism(PTE).Methods The clinical data of 163 consecutive PTE patients who were treated in our hospital from Jan.1972 to Dec.2006 were retrospectively reviewed.The patients were divided into group A and group B based on the time of application of standardized treatment and diagnosis for PTE.The clinical data of the two groups were analyzed and compared.Results The main risk factors included deep vein thrombus,operation,injury,fracture and tumors,etc.Dyspnea was the most important clinical symptoms.The incidences of dyspnea in group A and B were 92.5% and 84.6%,respectively.The diagnostic case and the ratio of final diagnosis in group B was increased compared to those in group A on an annual basis.The median time for diagnosis was shortened(P
7.The Effects of Ouabain on the Inner Ear Glial Cells in Mouse
Zhijian ZHANG ; Hongxia GUAN ; Kun YANG ; Bokui XIAO ; Hua LIAO ; Yang JIANG ; Tao ZHOU ; Qingquan HUA
Journal of Audiology and Speech Pathology 2017;25(5):502-505
To study the effects of ouabain on the inner ear glial cells, and to lay the foundation for the study of stem cell transplantation in the treatment of sensorineural hearing loss.Methods Sixty adult female SPF grade CBA / J mice were randomly divided into experimental group and control group, with 30 mice in each group.Animals in the experimental group received 3mM ouabain via the round window membrane, while mice in control group received normal saline.The mice were sacrificed at 7 days, 14 days and 30 days after the administration,respectively.Immunofluorescence histochemical staining was used to detect the inner ear glial cells in spiral ganglion.Results Some inner ear glial cells survived in the spiral ganglion of the experimental group, while with decreased numbers and disorganized structure compared to those of in the control group.Comparing to those of in the control group, the number and density of inner ear glial cells in the experimental group were significantly decreased from 7 days afterouabain administration,further decreased at 14 days and reduced to the lowest at 30 days after ouabain administration, the differences between the 2 groups were statistically significant (P < 0.05).Among the experimental group, the number of inner ear glial cells at 30 days was significantly decreased when compared to those of at 7 days and 14 days, respectively.Conclusion Application of ouabain to mouse inner ear via the round window membrane leads to an acute and progressive direct damage to the inner ear glial cells in the spiral ganglion.
8.Insulin and gliclazide therapies improve liver lipid deposition in type 2 di-abetic rats
Ding YUAN ; Hua LIANG ; Hongxia LIU ; Jing XU ; Fen XU ; Jinhua YAN ; Jianping WENG
Chinese Journal of Pathophysiology 2014;(6):1070-1075
AIM:To investigate the effect of insulin and gliclazide therapies on the liver fat accumulation in type 2 diabetic rats .METHODS:A high-fat diet plus low-dose streptozotocin was implemented to establish a type 2 dia-betic rat model, and the rats were randomly divided into diabetes mellitus (DM) group, diabetic rats treated with insulin ( INS) group, diabetic rats treated with gliclazide per os ( PO) group, and normal control ( NC) group.The diabetic rats in INS group and PO group were given insulin and gliclazide for 3 weeks, respectively.The changes of the liver fatty were evaluated with oil red O staining .Fasting plasma adiponectin concentration was measured by ELISA .The expression of adi-ponectin receptor 1 ( AdipoR1 ) was detected by real-time PCR.The protein levels of AMP-activated protein kinase (AMPK), phosphorylated AMPK on threonine 172 ( Thr172p-AMPK), sterol regulatory element-binding protein 1c (SREBP-1c), phosphorylated SREBP-1c on serine 372 (Ser372p-SREBP-1c), acetyl-CoA carboxylase (ACC), phospho-rylated ACC on serine79 (Ser79p-ACC) and immunoglobulin-binding protein (BiP) in the liver homogenate were deter-mined by Western blotting .RESULTS:Compared with the normal rats , in DM group, the presence of cytoplasmic lipid deposits was confirmed by oil red O staining .In INS group, these changes were significantly lower than those in DM group . Similar results were obtained in PO group .Insulin therapy significantly increased the plasma concentration of diponectin and liver tissue levels of AdipoR1 compared with DM group.At the same time, these 2 indicators returned to normal levels after gliclazide therapy .Thr172p-AMPK/AMPK, Ser372p-SREBP-1c/SREBP-1c and Ser79p-ACC/ACC expression ratios were significantly reduced in DM group compared with control values .The expression of BiP was increased on the contrary . After insulin therapy, Thr172p-AMPK/AMPK and Ser372p-SREBP-1c/SREBP-1c were significantly increased, and Ser79p-ACC/ACC and BiP returned to the normal levels .After gliclazide treatment, Thr172p-AMPK/AMPK and Ser372p-SREBP-1c/SREBP-1c returned to the normal levels , the expression ratio of Ser79p-ACC/ACC had no significant improve-ment compared with DM group , and the expression of BiP significantly declined .CONCLUSION: Both the insulin and gliclazide therapies reduce the lipid deposition in the liver of rats with type 2 diabetes by activating AMPK , but the extent and mechanism are not the same.In insulin therapy, AMPK restrains the expression of SREBP-1c directly, increases the phosphorylation of SREBP-1c, and affects SREBP-1c by inhibiting the endoplasmic reticulum stress .Gliclazide treatment, which has no effect on the lipid oxidation , reduces lipid deposition in the liver only through the phosphorylation of SREBP-1c and the suppression of the endoplasmic reticulum stress .
9.The TCM syndrome objectivity research on polycystic ovary syndrome of spleen-kidney yang deficiency type
Maohua LAI ; Hongxia MA ; Hua LIU ; Xinghua SONG ; Tao DING ; Yanhua ZHENG
International Journal of Traditional Chinese Medicine 2012;34(7):591-593
Objective To explore the essence of polycystic ovary syndrome of spleen-kidney yang deficiency,and to investigate the objective standardization of TCM syndrome diagnosis of the disease.Methods Among the 200 PCOS patients,the Chinese medicine syndrome pattern was differentiated as spleen-kidney yangdeficiency type (n=105 )and Non-spleen-kidney yang deficiency type (n=95).To measure and compare their levels ofBMI、WHR、sexual hormones、FINS、2 hINS,and HOMR-IR.Results The levels of BMI (24.4±2.8)、WHR (0.88±0.07)、LH/FSH (2.02±1.06)、E2 (55.84±23.87) pg/ml、HOMR-IR (2.95±1.52)、FINS (15.18±7.64) IU/ml、2hINS ( 59.53 ±40.61 ) IU/ml in the group of spleen-kidney yang deficiency type were obviously higher than those[ (22.2±2.2)、(0.80±0.06)、(3.50± 1.80)、(45.82±21.31) pg/ml、( 1.90± 1.50)、(9.93±6.59) IU/ml、(50.56±41.34) IU/ml respectively] in the group of Non-spleen-kidney yang deficiency type (P<0.05=,but T and FSH level showed no significant difference between two groups [FSH the group of spleen-kidney yang deficiency type=(6.46±0.95) IU/L、FSH the group of No-spleen-kidney yang deficiency type=(6.40±1.16) IU/L; T the group of spleen-kidney yang deficiency type=(0.81±0.15) μg/L、T the group of No-spleen-kidney yang deficiency type=(0.72±0.13) μg/L,P>0.05].Conclusion BMI、WHR、LH/FSH、E2、FINS、2HINS,and HOMR-IR could be used as objective index in identifying the difference in between spleen-kidney yang deficiency type and Non-spleen-kidney yang deficiency type.
10.Simultaneous Determination of Three Sulfonamides Residues in Vegetable by High Performance Liquid Chromatographic Method with Fluorimetric Detection
Xuede LI ; Qiming XIAN ; Hongling LIU ; Rimao HUA ; Shanshan XU ; Haiyan CHEN ; Hongxia YU
Chinese Journal of Analytical Chemistry 2010;38(3):429-433
A high performance liquid chromatographic method with fluorimetric detector was developed for the simultaneous determination of three sulfonamides in vegetable samples. Vegetable samples were extracted with methanol for three times, and then the combined extracts were evaporated to dryness under reduced pressure at 45 ℃. The residue was dissolved in 0.1 mol/L HCl and the analytes were derivatized with fluorescamine. The chromatographic separation was performed on an ODS column with a gradient elution program using mobile phases based on mixtures of acetonitrile and 0.5% acetic acid aqueous solution. The derivatized compounds were detected with fluorimetric detector. The limit of detection was 1.02-1.29 μg/L and the limit of quantification was 3.4-4.3 μg/kg(fresh weight, F.W.) for three sulfonamides in vegetable. The average recoveries were higher than 87%, inter and intra RSDs were lower than 10% for all samples spiked with 0.2-1.0 μg/g of sulfonamides. The proposed method has been applied to the analysis of vegetables sold in Hefei markets. The result indicated that 3 SAs were found at different degree in the practical vegetable samples with the total concentrations between 0.0726-0.3709 μg/g(F.W.).