Methods Acute attack of asthma patients with 84 patients, divided into virtual Asthma Group, the other card-based group, another non-choice of 63 patients with asthma, as a control group. Detection of all selected in the peripheral blood TXA2; TPA, PAI; blood coagulation. Results ①asthma group and control group case-control study results: OR=2.950, 95% CI (1.504, 5.785) exposure risk of the disease as non-exposed group of 2.95 times. ②Asthma group and the control group between the various indicators of hemorheology, in addition to TPA (P＞0.05) no significant difference. Ms indicators are significant differences (P＜0.05), suggested that asthma in the blood change detection indicators relative to the control group have changed significantly. Conclusion ①"Stasis" and asthma-related, "Stasis" Asthma is a major cause of morbidity. ②From stasis treatment of asthma, Huoxue Huayu Prescription provide the basis for the treatment of asthma.

Objective To systematically analyze and assess the risk of venous thromboembolism in the patients with rheuma‐toid arthritis(RA) .Methods The related literatures on the venous thromboembolism occurrence in the patients with RA published at home and abroad were performed the electronic retrieval .The obtained data were analyzed by adopting the RevMan5 .1 software . The data unable to merge were analyzed by adopting the descriptive analysis method .Results A total of 10 related papers were in‐cluded .The 3 indicators of venous thromboembolism occurrence rate ,deep vein thrombosis occurrence rate and pulmonary thrombo‐embolism occurrence rate were performed the meta analysis .The RR and 95% CI of the occurrence rates of venous thromboembo‐lism ,deep vein thrombosis and pulmonary thromboembolism were 2 .00(1 .71 ,2 .33) ,2 .31(1 .84 ,2 .90) ,2 .25(2 .23 ,2 .28) respec‐tively .Conclusion The risk degree of venous thromboembolism occurrence in the RA patients is higher than that in the non‐RA pa‐tients .

Objective To investigate the effects of atorvastatin and trimetazidine treatment of coronary heart disease (CHD) effect and influence on blood lipid levels.Methods Selected 103 CHD patients treated in our hospital from March 2013 to January 2016, were randomly divided into observation group (n=57) and control group (n=46), the observation group was given atorvastatin combined with trimetazidinetreatment, control group was given trimetazidine treatment, compared the two groupstherapeutic, blood lipid levels etc.Results The curative effect of observation group was significantly better than that of the control group ( P <0.05 ) , markedly effective was 54.39%, effective was 38.60%; The observation group after treatment blood lipid levels were lower than before treatment ( P <0.05 ); The control group after treatmenttotal cholesterol ( TC ) and low density lipoprotein cholesterol (LDL-C) compared with before treatment decreased (P<0.05), and three glycerol (TG) and high density lipoprotein cholesterol ( HDL-C) difference had no statistical significant compared with before treatment;The observation group after treatment TC, TG, HDL-C and LDL-C were lower than the control group (P<0.05);Observation group after treatment the duration of exercise, induced angina pectoris attack time and ST segment down 1 mm time were (624.59 ±90.15)s, (470.29 ±44.20)s and (455.04 ±82.05)s, significantly longer than that in control group (P<0.05);Observation group after treatment superoxide dismutase (SOD) was (87.82 ±8.84)U/mL, significantly higher than that of the control group (P<0.05), and malondialdehyde (MDA)was (4.02 ±0.70)nmol/mL, was significantly lower than that of the control group (P<0.05).Conclusion Atorvastatin combined with trimetazidine treatment CHD curative effect is better, can reduce the level of blood lipid and oxidative stress, is worthy of clinical use.

Objective To assess the effectiveness of the combined treatment model for Wilms'tumor and to improve treatment results.Methods Fifty-five patients diagnosed with Wilms' tumor between July 1981 to June 2010 were analyzed retrospectively.Eighteen patients were diagnosed by preoperative ultrasound-guided fine needle biopsy,and 53 patients were confirmed by postoperative pathology results.Seven cases were in clinical stage Ⅰ,19 cases in clinical stage Ⅱ,21 cases in stage Ⅲ,six cases in stage Ⅳ and two cases in stage Ⅴ.Thirty-five cases had histopathological subtype,30 cases had the favorable type,and five cases had the unfavorable type.Among the 55 patients,kidney tumor resection was performed on 48 cases,wide edge partial nephrectomy was performed on two cases,tumor enucleation was performed on one bilateral renal tumor case,kidney tumor resection with pulmonary metastasectomy was performed on two cases,and two cases had no surgical procedures.Eighteen cases received preoperative chemotherapy,40 cases received postoperative chemotherapy,and 12 cases received postoperative radiotherapy.Patients were grouped according to age,stage,histological type,treatment model,treatment course and whether or not they had radiotherapy.The Kaplan-Meier method was used in the evaluation and comparison of over survival (OS),disease free survival (DFS) and relapse free survival (RFS) of the different groups to reveal the relationship between different grouping factors with the prognosis of Wilms' tumor. ResultsThe median of follow-up was 34 mon ( ranging from 3 to 355 mon).The 3-year OS,5-year OS and 2-year DFS were 77.6％,69.0％ and 52.4％,respectively.The differences of OS in different stages ( P =0.006 ),DFS between pure operation group and combined therapy group ( P =0.004 ) and RFS between radiotherapy group and no radiotherapy group ( P =0.03 ) were significant,P ＜ 0.05.ConclusionsThe normative multi-disciplinary treatment model for patients with Wilms' tumor can achieve good results and is well tolerated.

Objective To explore the ultrasonographic characteristics of internal jugular vein(IJV) valve insufficiency and reflux.Methods 40 healthy volunteers' bilateral IJVs were study subjects.The inner diameter,valve morphological feature,reflux distance and hemodynamic parameters in IJV were detected by color Doppler ultrasound (CDU),the contrast agents transvalvular regurgitation in IJV was monitored by contrast-enhanced ultrasound (CEUS) (with low mechanical index,MI 0.10),then analyse the results of two methods.Results Of 80 investigated IJVs,1 vein was excluded due to absence of its left valve,so 79 veins were evaluated in the study.The IJV's inner diameter on the right side was larger than on the left side,but there was no significant difference (P ＞0.05).The detection rate of IJV valve insufficiency was 31.6％ (25/79),which was higher on the right than that on the left,but without significant difference (P ＞ 0.05).The detection rate of IJV valve insufficiency was significantly higher in the group of greater than or equal to 50 years old than that in less than 50 years old(P ＜0.05).The reflux distance in IJV displayed by CDU was associated with IJV valve's function.Compaired with sufficient valves,the reflux duration time in insufficient valves during quiet respiration showed no significant difference (P ＞0.05).During Valsalvalike manoeuvres and quiet respiration,the reflux duration time in sufficient valves showed no significant difference (P ＞ 0.05).The reflux duration time in insufficient valves during Valsalva-like manoeuvres showed significantly higher than that during quiet respiration (P ＜0.01).Compaired with sufficient valves,the peak flow velocity of reflux flow in insufficient valves was significantly prolonged during quiet respiration and Valsalva-like manoeuvres (P ＜0.05,P ＜0.01,respectively).Conclusions The incidence rate of IJV valve insufficiency and reflux is high in healthy individuals.During quiet respiration and/or Valsalva-like manoeuvres,detecting the reflux distance,reflux duration time and peak flow velocity of reflux flow by CDU are valuable in evaluating IJV valve insufficiency and the degree of IJV reflux.

Aim To observe the analgesic effect of oxymatrine(OMT)and its mechanism.Methods A peripheral mononeuropathy was produced in adult mice by placing loosely constrictive ligatures around the common sciatic nerve.The antinociceptive effects of the OMT were assessed in mechanical allodynia and cold allodynia tests.The CAMKII inhibitor KN-93 and AIP were adopted to investigate the influence of OMT on the analgesic effect and analyze its analgesic mecha-nisms.Western blot was used to evaluate the expres-sions of tCaMKII and pCaMKII protein.Results The intraperitoneal administration of OMT (1 60,80 mg· kg -1 )increased the paw withdrawal threshold in the
mechanical allodynia test (P <0.05 ),OMT (1 60, 80,40 mg·kg -1 ,ip)remarkably decreased the paw lifts in the cold allodynia test (P <0.05).Ith KN-93 (1 .25 μg/site),AIP (0.02 μg/site)significantly en-hanced the analgesic effect of OMT (35 mg·kg -1 ) (P <0.01 ).Protein expression of pCaMKII was de-creased by OMT(1 60 mg·kg -1 ).Conclusion OMT has significant protective effects on chronic constriction injury(CCI)in mice,and the effective mechanism of OMT inhibits the expression of CaMKII receptor.

Objective To evaluate the efficacy of left ventricular ejection time (LVET) in guiding the volume management during liver transplantation.Methods Sixty American Society of Anesthesiologists physical status Ⅱ or Ⅲ patients,aged 32-64 yr,weighing 54-93 kg,of Child-Pugh grade A or B liver function,scheduled for elective the first liver transplantation with general anesthesia,were divided into either control group (group C) or transesophageal echocardiography (TEE) monitoring group (group TEE),with 30 patients in each group.In group C,the fluctuating range of mean arterial pressure,heart rate and central venous pressure was maintained less than 20％ of the baseline value,and the urine volume was maintained ＞1 ml · kg-1 · h-1.LVET was maintained between 0.35-0.40 s in group TEE.The consumption of intraoperative vasoactive agents (dopamine,norepinephrine,epinephrine),volume of fluid infused,volume of blood transfused,blood loss and urine volume were recorded.The occurrence of adverse events was observed during the perioperative period,and postoperative extubation time and intensive care unit residence time were also recorded.Results Compared with group C,the consumption of intraoperative dopamine and norepinephrine was significantly decreased,the urine volume was increased,the incidence of myocardial ischemia,pulmonary edema and renal failure in the perioperative period was decreased,and the postoperative extubation time and intensive care unit residence time were shortened in group TEE (P＜0.05).There was no significant difference between the two groups in the volume of crystalloid,colloid,red blood cells and plasma infused or blood loss (P＞0.05).Conclusion LVET produces good efficacy in guiding the volume management during liver transplantation.

Objective To evaluate different detection methods in the diagnosis of severe fever with thrombocytopenia syndrome (SFTS), and find the most quick and accurate one for the identification of new bunyavirus infection. Methods Real-time PCR and ELISA-IgM were used to detect serum samples of 158 patients with acute phase of SFTS, which were collected from the special monitoring system of SFTS in Henan Province in 2014. IgM and IgG antibodies were detected by ELISA in 109 acute and convalescent paired serum specimens. The differences of the positive rates were compared between the three methods, and the influence of the collected interval time on the detection results was analyzed. Results For 158 acute phase serum samples of SFTS patients, the positive rate detected by real-time PCR (76.58%) was higher than that of ELISA-IgM (47.47%), and the difference was statistically significant (χ2=34.13, P < 0.05). For 109 cases with acute and convalescent paired serum samples, there was no significant difference in the positive rates between ELISA-IgG ( 75.23%) and real-time PCR (72.48%) detections (χ2=0.18, P>0.05). In both the acute phase and convalescent phase, the positive rate of IgM was higher than that of IgG, and the difference was statistically significant (χ2=41.68 and 6.25, P<0.05). With the extension of collected interral time, the positive rates of IgM and IgG antibodies were both increased ( Z=6.42 and 10.08, P < 0.05). Conclusion Real-time PCR is the most sensitive method for the early diagnosis of the SFTS. ELISA-IgG is suitable for the detection of SFTS at recovery period. ELISA-IgM can be used as an assistant method to guide clinical diagnosis.

AIM: To test the effect of ERK1/2 on ischemic preconditioning (IPC) in diabetic rat hearts. METHODS: The diabetic rat model was made with alloxan. After eight weeks, 24 rats were divided into 4 groups: non-diabetic IPC rats (group A); non-diabetic non-IPC rats (group B); diabetic IPC rats (group C); diabetic non-IPC rats (group D). ECGⅡ lead, left ventricular development pressure (LVDP), and first derivative of LVDP ~(?dp/dt_~max ) were recorded. Myocardial phosphorylation of extracellular signal regulated kinases1/2 (ERK1/2) was detected by Western-blotting. RESULTS: (1) The ventricular arrythmia score was significantly lower in group A than that in group C (P