OBJECTIVE:To study the effects of Yangrong runchangshu mixture on tyrosine kinase receptor protein CD117 (c-Kit),substance P(SP)and myenteric nerve plexus vasoactive intestinal peptide(VIP)in the colon interstitial cell of Cajal of rat with slow transit constipation(STC). METHODS:Morphine hydrochloride(2.5 mg/kg)was given sc once daily for 45 consecu-tive days to establish STC rat models. 120 rats were randomized into a normal control (isometric normal saline) group,a model (isometric normal saline)group,Maren pill(positive control,14.7 g/kg)group and Yangrong runchangshu mixture high-dose and low-dose [15 and 7.5 g(medicinal materials)/kg] groups. After successful establishment of the models,drugs were given ig once dai-ly for 10 consecutive days. Immunohistochemical method was employed for staining. The changes in the structure and c-Kit,SP and VIP expressions in rats’colonic tissues were observed under the light microscope. RESULTS:Compared to the normal control group,those in the model group demonstrated weaker expressions of c-Kit and SP and less positive cells in colon,stronger expres-sion of VIP and more positive cells,with statistical significances (P<0.01);cellular morphology became abnormal obviously. Compared to the model group,those in Maren pill group and Yangrong runchangshu mixture high-dose and low-dose groups showed stronger expressions of c-Kit,SP and more positive cells in colon,weaker expressions of VIP and less positive cells with statistical significances (P<0.05);cellular morphology was improved to some extent. CONCLUSIONS:Yangrong runchangshu mixture can improve the contents of SP and VIP and the expression of c-Kit in rats with STC to some extent.

To investigate the effect of treasure pill pretreatment on hypoxic brain injury Fas and Fas L protein expression.Methods:Newborn 7d Wistar rats 200,male or female,were randomly divided into 5 groups(n=40):normal control group, model group 1h hypoxia(Ⅰm-12h,Ⅰm-24h,Ⅰm-48h,Ⅰm-72h),model group 4h hypoxia(Ⅳm-12h,Ⅳm-24h,Ⅳm-48h,Ⅳm-72h), hypoxic 1h treasure pill treatment group(ⅠT-14h,ⅠT-24h,ⅠT-48h,ⅠT-72h),hypoxic 4h treasure pill treatment group(ⅣT-14h,ⅣT-24 h ,ⅣT-48 h ,ⅣT-72 h ).The newborn Wistar rats were placed in hypoxia chamber of 8%O2+92%N2 ( V/V) for establishment the model of neonatal rats with hypoxia.Before modeling continuous 7 d once-daily oral administration of saline and treasure pill , during hypoxia also dose ,after hypoxia 12 h,24 h,48 h,72 h animals were sacrificed blood and brain tissues tested.By ELISA to detect the protein levels of Fas and Fas L in serum and brain tissue;by immunohistochemistry method to detect the expression of Fas and Fas L in rat brain tissue.Results:Compared with normal control group ,other four groups the protein content and protein expression of Fas and Fas L are in the 12h time points after hypoxic significantly increased ,and 24 h time point that pealed at statistically significant ( P<0.01 ) ,model group was significantly higher than the treatment group at each time point ;model groups in 48 h,72 h time point the protein expression and protein content of Fas and Fas L was less than 12 h time point but still significantly higher than the control group ( P<0.01 );treasure pill groups in 48 h,72 h time point the protein expression and protein content of Fas and Fas L was less than 12 h time point ,and slightly higher than the normal control group ( P<0.05 ).Conclusion: Treasures pill can cut hypoxic brain damage in rats,the positive expression of Fas and Fas L protein content and protein ,and thus the hypoxic brain injury has a protective effect.

[Objective]To study the changes of biomechanical efficiency of absorbable balloon vertebraplasty associated with pedicle screw system for treatment of thoracolumbar fracture.[Method]Six fresh adult thoracolumbar specimens were harvested and 10 segmental specimens(T_(11)~L_1、 L_(2~4)、 L_(1~3)) were processed.After burst fracture created by free-drop test,the specimens were braced and reduced by APF pedicle screw,then absorbable balloon were placed through the traumatic vertebral pedicle and were infused with autosolidification calcium phosphate cement to process vertebroplasty.The stiffness in middle vertebrae were measured under flexion,extension,lateral flexion and torsion stress before fracture and after absorbable balloon vertebroplasty respectively.[Result]The crushing strength in traumatic vertebrae after vertebroplasty with absorbable saeculi increased showed 8% under flexion,15% under extension and 9.8% under lateral flexion,but with significant increasing under extension(P0.05).[Conclusion](1)absorbable balloon vertebraplasty associated with pedicle screw system conduces to the reconstruction of traumatic vertebrae,and making postoperative spinal biomechanical characteristics approach the level before fracture;(2)Vertebroplasty with absorbable sacculus for treatment of thoracolumbar burst fracture based on the premise that avoid the potential danger induced by leakage of bone cement,and can reestablish intact corporeal structure of traumatic vertebrae and restore their height essentially,so that spinal physiologic curve can be gained to restore and maintain,tardive lumbodorsal pain and spinal cord injury and nerve injury caused by secondary kyphosis can be prevented,this can fundamentally solve complications left over by thoracolumbar burst fracture treated using pedicle screw system simply.The new method prorides a rational method for clinical treatment of thoracolumbar burst fractures.

Objective To evaluate the diagnosis and treatment of pseudoaneurysm .Methods The clinical data of 17 cases of pseudoaneurysm from Jan .2007 to Dec .2011 were reviewed retrospectively ,10 cases were caused by iatrogenic ,6 cases were drug caused by injection of poison ,and 1 case was was caused by trauma .2 cases of brachial artery pseudoaneurysm ,and 15 cases of arte-ria femoralis pseudoaneurysm .1 case was treated by simple compressive therapy ,the other 16 underwent surgical treatment and the pseudoaneurysm was removed ,in which 3 cases were treated by artery patch repair ,7 were treated by bypass with prosthetic grafts , 2 were treated by bypass with saphenous vein ,4 were treated by bypass with superficial femoral vein branch .Results There was no death except 1 case died because her family gave up ,All patients with signs and symptoms were significantly alleviated ,and there was no severe ischemia limb leading to amputation in postoperative follow-up .Conclusion The revascularization of the artery in pseudoaneurysm patients can make a significant improvement in the long-term quality of life .

This study aimed at exploring the effects of Griffipavixanthone,a natural small molecule extracted from Garcinia esculenta,on the autophagy and the mechanism behind it.Cervical cancer HeLa cells were selected as the model in vitro.Confocal microscopy,flow cytometry and immunoblotting were performed to know the impacts of Griffipavixanthone on signaling pathway over autophagy flux and calcium level;while nude mouse model was established to explore its anti-cancer action in vivo.It was found that Griffipavixanthone significantly induced GFP-LC3 puncta formation and the accumulation of LC3-Ⅱ/LC3-Ⅰ,and increased the protein expression of SQSTM1 in HeLa cells in a time and concentration dependent manner.MTT assay results showed that Griffipavixanthone inhibited the growth of HeLa cells in a dosage dependent manner,and eliminated the tolerance of tumor cells to nutrient starvation,which fastened the death of cells.Griffipavixanthone also down-regulated the concentration of calcium in the cytoplasm.Furthermore,the in vivo study indicated that intratumoral injection of Griffipavixanthone presented the anti-cancer effect to a certain extent.In conclusion,it was suggested that Griffipavixanthone inhibit autophagy with the potential to be developed into a clinical anti-cancer agent.Griffipavixanthone sensitized HeLa cells to nutrient stress-induced cell death through inhibiting the autophagy flux and the level of calcium in the cytoplasm.

Object To study the effect of plant growth regulator on medicinal constituents of Dendrobrium nobile Lindl Methods The root of D nobile was treated by immersing in solutions of plant growth regulator 6 BA and GA 3 Results and Conclusion Both plant growth regulator at certain concentration, resulted in an increase of total soluble sugar content, but without effect on the content of dendrobine GA 3 can significantly elevate the content of total alkaloid in D nobile

Objective To investigate the effects and mechanism of retinoie acid on B16 marina mel-anoma cells and human melanocytes in vitro. Methods B16F10 murine melanoma cells and human mela-noeytes were cultured in culture medium which contains different concentration of components, including retinoic acid. Using reverse transcription-polymerase chain reaction (RT-PCR) mRNA expression of the tyrosinase was detected. Tyrosinase activity, melanin content and cell proliferation rate were also deter-mined. Results Retinoieacid exhibited an inhibitory effect on the expression of tyrosinase mRNA. As the concentration of retinoic acid was 100 μmol/L, treating for 72 h, the expression of tyrosinase mRNA de-creased 30.13 %, retinoic acid exhibited an inhibitory effect on tyrosinase activity and melanin production at high concentration (>500 μmol/L), and it could promote the cell proliferation. Retinoic acid and hy-droqninone could be cooperative at high concentration (1 000 μmol/L), and enhanced the down regulation of tyrosinase activity and melanin content. Retinoic acid could also mitigate the inhibitory effect of hydro-quinone on cell proliferation, so as to protect the cells from injury. Hydroquinone had no effect on tyrosi-nase gene expression at mRNA level. Conclusion Retinoic acid inhibits the synthesis of melanin by the genetic regulation at mRNA level.

Objective To establish a method of nucleic acid extraction and enrichment based on magnetic nanoparticle as medium for elevating the analytical sensitivity of domestic HBV real-time PCR kit and detection of the trace amount HBV DNA. Methods After receiving antiviral treatment, the serum samples of 50 hepatitis B patients with HBV DNA concentration ≤1×104 IU/ml were collected. The WHO HBV DNA calibrator was used as the standard material. Nanometer magnetic beads were used to adsorb and enrich the HBV nucleic acid and increase the concentration of the extracted HBV nucleic acid template. Compared with Roche HBV DNA detection reagent and four domestic reagent with conventional nucleic acid extraction and detection method, the improvement effect of this method on domestic nucleic acid detection reagent was evaluated. Results After application of nanometer magnetic extraction method to domestic regent, the analytical sensitivities of the domestic reagent reached 10 and 50 IU/ml, respectively,which was about the same detection level to 12 IU/ml of the imported Roche reagent. The positive rates of the detection of serum trace amount HBV DNA of hepatitis B patients with four kinds of domestic extraction reagent were 64% ( 32 ), 56% ( 28 ), 62% ( 31 ) and 58% (29), respectively. There were significant statistical differences between Roche reagent and four domestic extraction reagent kits(x2 = 7. 895, 12. 698,9. 013 and 11. 416 ,P ＜0. 05 ). With nanometer magnetic extraction method combined with domestic reagent kits, the detection rates were 88% (44), 88% (44), 88% (44) and 86% (43) ,respectively. There was no significant difference compared with the imported Roche reagent (x2 = 0. 000, 0. 000, 0. 000 and 0. 088,P ＞0. 05). Moreover, when the HBV nucleic acid concentration was 101-103 IU/ml, the logarithm value of viral nucleic acid concentration was in reverse correlation to Ct value, but the correlation decreased in the concentration range of 103-106 IU/ml. Conclusions The nucleic acid extraction method based on magnetic nanoparticle as medium can significantly improve the analytical sensitivity of domestic HBV DNA detection reagent, which can be used to monitor the trace amounts HBV DNA in the sera of the hepatitis B patients.

Through the addition of discussion course associated with judicial expertise during the pre medical education, integration of true and typical forensic pathological cases into basic medical theory and experimental education, further addition of optional course of forensic medicine,and guiding the medical students applying the scientifically training projects about forensic pathology, students may improve their learning interesting and clinical thought, and are made early warning and increase the abilities of preventing and dealing with the suddenly medical tangles in the future, at the same time, the medical teachers also increase their professional levels and teaching qualities.These benefit the growing up of high quality medical doctors, decrease and even prevent the happening of medical tangles.

Objective To evaluate a new gene therapy for the treatment of experimental occlusive arterial disease Methods Magnetic nanospheres were produced, VEGF gene was cloned for subsequent construction of eukaryotic expression plasmid The magnetic gelatin microspheres used in targeted gene therapy were prepared by emulsion crosslinking method The microspheres were injected intrafemorally in rabbits through contralateral femoral artery, and the ischemic limb was placed in a magnetic field Angiography was performed on day 10 and day 30 respectively The capillary density and the capillary to muscle fiber ratio were determined histochemically Results Compared to the controls there was significant collateral artery development in VEGF transfected group The capillary density and the capillary to muscle fiber ratio were significantly higher for the VEGF transfected group than for the control group. The capillary density of control was (125?23)/mm 2, and in VEGF group was (298?27)/mm 2, P