BACKGROUND: Cryoimmunology has been paid more attention in recent years, but the effect of cryoablation on cellular function has not been clearly understood, especially for the cellular apoptosis and dendritic cells.OBJECTIVE: To evaluate whether NCI-H446 lung cancer cells could activate bone marrow-derived dendritic cells (DCs) which stimulate lymphocytes to be cytotoxic lymphocytes (CTLs) to kill cancer cells?DESIGN, TIME AND SETTING: Cellular immunology experiment was performed at the Hematological Laboratory, Beijing Naval General Hospital, and Genetics Center of Military Medical Science Academy of the PLA between August 2002 and April 2003.MATERIALS: NCI-H446 cells were small lung cancer cells purchased from Shanghai Institute of Sciences.METHODS: Cultured lung cancer cells were cryo-treated and added to cultured DCs in the presence of naive lung cancer cells. Morphological changes of cancer cells were observed; mixed lymphocyte reaction (MLR) and antitumor activity of cytotoxic T lymphocyte (CTLs) were also evaluated.MAIN OUTCOME MEASURES: Morphology of DCs and cell surface immunophenotype changes; MLR of cultured cells and cell apoptosis.RESULTS: ① of 51 patients,18 were followed-up for 6-12 months,2 for 13-18 months,and 8 for 19-24 months.② A total of 49 (96.1%) out of 51 patients preserved the eyeballs and the visual acuity improved to different degrees in 48 (94.1%) patients.③ After surgery,fungal infection recurred in 6 eyes (11.6%),4 of which were controlled by antifungal medication and 2 was enucleated because of uncontrolled endophthalmitis.Graft rejection was found in 18 (35.3%) eyes, 13 of which recovered transparent by medication and 5 received secondary PKP.Graft ulceration was present in 4 (7.84%) eyes, 3 of which were cured and the remaining one was re-grafted because of severe endothelial cell loss. Secondary glaucoma appeared in 7(13.7%) eyes,and the intraocular pressure was controlled medically and surgically.Complicated cataract occurred in 6 (11.8%) eyes,3 of which underwent cataract extraction. Most complications were successfully controlled.In the final follow-up period,45 (88.2%) grafts were transparent.CONCLUSION: Cryo-treated lung cancer cells exhibited cell lysis and membrane collapse, which activate bone marrow-derived DCs to kill cancer cells specifically.

Objective To compare the clinical efficacy of laparoscopic and open method in the treatment of gastric cancer radical surgery.Methods 1 1 0 patients who underwent radical surgery for gastric cancer were selected. According to its different operation method,they were divided into observation group and control group,55 cases in each group.The observation group received laparoscopic surgery,and the control group received open operation treat-ment.The general surgery situation,the C -reactive protein (CRP)levels at preoperation and postoperative 1 ,7,1 0d were compared in the two groups.Results The surgical incision length,intraoperative blood loss,postoperative bowel movements and postoperative hospital stay in the observation group were (24.6 ±3.7)cm,(99.9 ±31 .8)mL,(5.8 ± 2.7)d,(1 2.8 ±7.7)d respectively,which were significantly lower than (5.6 ±1 .4)cm,(61 .3 ±1 3.6)mL,(4.5 ± 0.7)d,(9.2 ±3.9)d in the control group(t =31 .472,5.289,3.1 94,2.398,all P <0.05),the number of the lymph node in the two groups had no significant difference(P >0.05).CRP level at 1 d preoperation,1 d postoperation,7d postoperation,1 0d postoperation of the control group were (1 8.21 ±3.67)mg/L,(42.96 ±7.28)mg/L,(22.88 ± 4.35)mg/L,(15.89 ±4.34)mg/L respectively,which of the observation group were (17.82 ±3.57)mg/L,(29.97 ± 4.58)mg/L,(1 6.01 ±3.1 6)mg/L,(8.65 ±1 .77)mg/L respectively,it had no significant difference between the two groups at preoperative 1 d (P >0.05 ),postoperative 1 d both elevated obviously,postoperative 7 d,1 0 d both significantly dropped than postoperative 1 d,and the differences between the two groups were significant (t =7.497, 6.521 ,8.339,all P <0.05).The incidence rate of complications was 5.45%(3 /55)in the observation group,which of the control group was 1 8.1 8%(1 0 /55),the difference between the two groups was statistically significant(χ2 =6.371 ,P <0.05).Conclusion Compared with the traditional laparotomy,laparoscopic gastric cancer radical has mild trauma,rapid recovery,the effect is good,quite cure degree,which is worthy of clinical popularization and application.

Some of the clinical manifestation of primary immunodeficiency disease in children is similar with that of hematological system diseases in children.It would result into the change of one or multiple lines of blood cells, which would lead to infection or malignant tumors.To strengthen the awareness of the hematologic manifestations of immunodeficiency diseases will be beneficial for doctors in the diagnosis of diseases,judgment of complication and prog-nosis.

This article based on the experience of LIN Lan’s clinic and scientifi c research,analysis the etiological factor and pathogenesis of diabetes mellitus(DM)complicating coronary heart disease(CHD),presumes that defi ciency of both qi and yin,blood stasis and sputum are mainly pathogenesis of DM complicating CHD.Professer LIN proposes the TCM treatment based on syndrome differentiation,which obtains obviously therapeutic effect.

As the economic reformation goes on, the traditional culture and ethics anr chanllenged. Should we carry on the spirit of Betheune? The hospital, cultural development focuses on how to carry forward the spirit of Betheune. Acording to his experience, the author explained his view and put several points that we should notice in new situation. 1. Emancipate the mind and change the view; 2. Advocate the unselfish contribution combined with the policy of contribution considering oneself; 3. Requine the level of conbined with regulatiton.

Objective:To compare the effect of curing polymyalgia rheumatica either by using both Biqi capsule and prednisone or by using prednisone alone. Methods: Thirty-nine patients of polymyalgia rheumatica were randomly divided into two groups, 18 patients in the treatment group treated with Biqi capsule and small dosage of prednisone; 21 patients in the control group treated with small dosage of prednisone. The therapeutic course was 12 weeks. The patients' symptoms of muscle ache, inflexibility in the morning and T-cell subsets determination (CD4, CD8)were observed and scores of clinical practice was made. Results: The respective total effective rate was 94.44% for the treatment group and 66.67% for the control group. There was distinctive difference in the scores for the treatment group and the control group in relieving the patients of the symptoms of polymyalgia rheumatica. The treatment group would help the patients of polymyalgia rheumatica to improve their immunity ability. Conclusion: The combination of Biqi capsule and prednisone can help to improve the effect of curing polymyalgia rheumatica.

Perioperative blood transfusion therapy for coagulation factor deficiency in children is very important to save patient's life or conducive to the postoperative recovery.With the advances in transfusion medicine,the cooperation through pediatric hematologists and surgeons in the perioperative period,more and more operation of children accompany coagulation factor deficiency can be safety finished.Different types of coagulation factor deficiency in children are treated by high-purity clotting factor,in order to correct abnormal coagulation factors levels.Clinical data showed that coagulation factor deficiency in children after proper treatment can be safely performed surgical procedure.

Objective To discuss the effective method for decreasing operative complications in acetabular fractures. Methods A retrospective analysis was done on 70 patients with acetabular fractures treated with open reduction and internal fixation from June 1998 to June 2003 so as, in combination of follow up data, to investigate cause, mechanism, and effective preventive and therapeutic methods. Results Open reduction and internal fixation were effective methods for acetabular fractures but had too many complications especially in traumatic arthritis and femoral head avascular necrosis, to which we must pay much more attention. Conclusions Operative injury lessening and anatomical reduction are keys to decrease of operative complications. Anterior screw fixation through inguinal approach may be an effective method of decreasing operative injury and complications.

Objective To assess the influence of different methods of blood sampling on the results detected by instant glucose monitor.Methods We searched the following electronic databases CNKI and VIP(from 1995 to 2007).Five quasi-RCTs involving 305 participants were included,RevMan 4.2.10 was used for statistical analysis.Results There was significance in results between instant glucose monitor and auto-analyzer by finger massage and extrusion(WMD=-1.32,95%CI:-2.05 to-0.59,WMD-2.11,95%CI:-2.95 to-1.27,respectively),but no difference by natural outflow(WMD=-0.17,95%CI:-0.53 to 0.19).Conclusion There are influence on the blood glucose results by different methods of blood sampling,the natural blood outflow of fingers is the better way.

Objective:To evaluate the curative effect of mirror therapy combined with rehabilitation training in the treatment of stroke hemiplegic upper limb motor dysfunction. Methods:To choose 48 patients, were randomly divided into control group, 24 cases in each group to observe, are basic rehabilitation training, observation group joint mirror therapy, using FMA, ARAT, BMI, respectively, evaluation of patients with general motor function, upper limb motor function, daily life ability, numerical measurement of radial carpal extensor emg. Results:After the treatment, the control group, the observation group the FMA level, ARAT level, MBI level, EMG level was higher than that before treatment, the observation group than that in the control group, the observation group FMA, ARAT, MBI, the difference of EMG signals is higher than that of control group, with significant difference(t=14.14,t=4.55,t=5.84, t=9.14;P<0.05).Conclusion: mirror therapy combined with rehabilitation training is really helpful to upper extremity motor functional recovery of stroke patients with hemiplegia, is of positive significance to improve the living ability of the patients.